CHC1

Summary

Gene Symbol: CHC1
Description: clathrin heavy chain
Alias: SWA5, clathrin heavy chain
Species: Saccharomyces cerevisiae S288c
Products:     CHC1

Top Publications

  1. Fernandez G, Payne G. Laa1p, a conserved AP-1 accessory protein important for AP-1 localization in yeast. Mol Biol Cell. 2006;17:3304-17 pubmed
    ..Deletion of LAA1, when combined with a conditional mutation in clathrin heavy chain or deletion of GGA genes, accentuated growth defects and increased disruption of clathrin-dependent alpha-..
  2. Newpher T, Lemmon S. Clathrin is important for normal actin dynamics and progression of Sla2p-containing patches during endocytosis in yeast. Traffic. 2006;7:574-88 pubmed
    ..These data demonstrate an important role for clathrin in organization and progression of early endocytic patches to the late stages of endocytosis. ..
  3. Di Pietro S, Cascio D, Feliciano D, Bowie J, Payne G. Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. EMBO J. 2010;29:1033-44 pubmed publisher
    ..These results define a novel mechanism for negative regulation of clathrin binding by an adaptor and suggest a role for SAM domains in clathrin-mediated endocytosis. ..
  4. Wendland B, McCaffery J, Xiao Q, Emr S. A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15. J Cell Biol. 1996;135:1485-500 pubmed
    ..This new screening strategy demonstrates a role for She4p and Pan1p in endocytosis, and provides a new general method for the identification of additional endocytosis mutants. ..
  5. Stepp J, Pellicena Palle A, Hamilton S, Kirchhausen T, Lemmon S. A late Golgi sorting function for Saccharomyces cerevisiae Apm1p, but not for Apm2p, a second yeast clathrin AP medium chain-related protein. Mol Biol Cell. 1995;6:41-58 pubmed
    ..In yeast containing a normal clathrin heavy chain gene (CHC1), disruptions of the APM genes, singly or in combination, had no detectable phenotypic ..
  6. Mayor T, Graumann J, Bryan J, MacCoss M, Deshaies R. Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway. Mol Cell Proteomics. 2007;6:1885-95 pubmed
    ..This approach illustrates the feasibility of systems-level quantitative analysis to map enzyme-substrate networks in the UPS. ..
  7. Copic A, Starr T, Schekman R. Ent3p and Ent5p exhibit cargo-specific functions in trafficking proteins between the trans-Golgi network and the endosomes in yeast. Mol Biol Cell. 2007;18:1803-15 pubmed
    ..We conclude that the clathrin adaptors AP-1, Ent3p, Ent5p, and the Ggas cooperate in different ways to sort proteins between the TGN and the endosomes. ..
  8. Rad M, Phan H, Kirchrath L, Tan P, Kirchhausen T, Hollenberg C, et al. Saccharomyces cerevisiae Apl2p, a homologue of the mammalian clathrin AP beta subunit, plays a role in clathrin-dependent Golgi functions. J Cell Sci. 1995;108 ( Pt 4):1605-15 pubmed
    ..have been engineered in cells expressing wild-type (CHC1) or temperature sensitive (chc1-ts) alleles of the clathrin heavy chain gene...
  9. Peng J, Schwartz D, Elias J, Thoreen C, Cheng D, Marsischky G, et al. A proteomics approach to understanding protein ubiquitination. Nat Biotechnol. 2003;21:921-6 pubmed
    ..The methodology described here provides a general tool for the large-scale analysis and characterization of protein ubiquitination. ..

More Information

Publications64

  1. Aguilar R, Watson H, Wendland B. The yeast Epsin Ent1 is recruited to membranes through multiple independent interactions. J Biol Chem. 2003;278:10737-43 pubmed
    ..These events may in turn favor the occurrence of other interactions, for instance EH-NPF recognition, thus stabilizing networks of low affinity binding partners at endocytic sites. ..
  2. Valdivia R, Baggott D, Chuang J, Schekman R. The yeast clathrin adaptor protein complex 1 is required for the efficient retention of a subset of late Golgi membrane proteins. Dev Cell. 2002;2:283-94 pubmed
    ..Similarly, in AP-1 deficient cells, the resident TGN/early endosome syntaxin, Tlg1p, is missorted. We propose that clathrin and AP-1 act to recycle Chs3p and Tlg1p from the early endosome to the TGN. ..
  3. Costaguta G, Stefan C, Bensen E, Emr S, Payne G. Yeast Gga coat proteins function with clathrin in Golgi to endosome transport. Mol Biol Cell. 2001;12:1885-96 pubmed
    ..growth and alpha-factor maturation defects in cells carrying a temperature-sensitive allele of the clathrin heavy chain gene...
  4. Phan H, Finlay J, Chu D, Tan P, Kirchhausen T, Payne G. The Saccharomyces cerevisiae APS1 gene encodes a homolog of the small subunit of the mammalian clathrin AP-1 complex: evidence for functional interaction with clathrin at the Golgi complex. EMBO J. 1994;13:1706-17 pubmed
    ..growth and alpha-factor pheromone maturation defect of cells carrying a temperature-sensitive allele of clathrin heavy chain (Chc) (chc1-ts)...
  5. Wendland B, Emr S. Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis. J Cell Biol. 1998;141:71-84 pubmed
    ..We suggest that Pan1p (and by extension eps15) serves as a multivalent adaptor around which dynamic interactions between structural and regulatory components of the endocytic pathway converge. ..
  6. Wendland B, Steece K, Emr S. Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis. EMBO J. 1999;18:4383-93 pubmed
    ..Based on these and other data, we propose that the yeast epsin-like proteins are essential components of an endocytic complex that may act at multiple stages in the endocytic pathway. ..
  7. Chu D, Pishvaee B, Payne G. The light chain subunit is required for clathrin function in Saccharomyces cerevisiae. J Biol Chem. 1996;271:33123-30 pubmed
    ..examine Clc function, clc1Delta strains were generated that carry a multicopy plasmid containing the clathrin heavy chain gene (CHC1), resulting in levels of Chc 5-10-fold elevated over wild-type levels...
  8. Gall W, Geething N, Hua Z, Ingram M, Liu K, Chen S, et al. Drs2p-dependent formation of exocytic clathrin-coated vesicles in vivo. Curr Biol. 2002;12:1623-7 pubmed
    ..An arf1 synthetic lethal screen identified DRS2/SWA3 along with a clathrin heavy-chain conditional allele (chc1-5/swa5-1) and SWA2, encoding the yeast auxilin-like protein involved in uncoating CCVs...
  9. Yeung B, Payne G. Clathrin interactions with C-terminal regions of the yeast AP-1 beta and gamma subunits are important for AP-1 association with clathrin coats. Traffic. 2001;2:565-76 pubmed
    ..The combination of subunit mutations accentuated growth and alpha-factor pheromone maturation defects in chc1-ts cells, though not to the extent caused by complete loss of AP-1 activity...
  10. Pishvaee B, Costaguta G, Yeung B, Ryazantsev S, Greener T, Greene L, et al. A yeast DNA J protein required for uncoating of clathrin-coated vesicles in vivo. Nat Cell Biol. 2000;2:958-63 pubmed
    ..Our results demonstrate an in vivo uncoating function of a J domain co-chaperone and establish the physiological significance of uncoating in transport mediated by clathrin-coated vesicles...
  11. Bensen E, Costaguta G, Payne G. Synthetic genetic interactions with temperature-sensitive clathrin in Saccharomyces cerevisiae. Roles for synaptojanin-like Inp53p and dynamin-related Vps1p in clathrin-dependent protein sorting at the trans-Golgi network. Genetics. 2000;154:83-97 pubmed
    ..mutations that display synthetic growth defects when combined with a temperature-sensitive allele of the clathrin heavy chain gene (chc1-521) in Saccharomyces cerevisiae...
  12. Yeung B, Phan H, Payne G. Adaptor complex-independent clathrin function in yeast. Mol Biol Cell. 1999;10:3643-59 pubmed
    ..Individual or combined disruption of AP-1 subunit genes in cells expressing a temperature-sensitive clathrin heavy chain results in accentuated growth and alpha-factor pheromone maturation defects, providing further evidence ..
  13. Chen C, Graham T. An arf1Delta synthetic lethal screen identifies a new clathrin heavy chain conditional allele that perturbs vacuolar protein transport in Saccharomyces cerevisiae. Genetics. 1998;150:577-89 pubmed
    ..Here, we report that swa5-1 is a new temperature-sensitive allele of the clathrin heavy chain gene (chc1-5), which carries a frameshift mutation near the 3' end of the CHC1 open reading frame...
  14. Pishvaee B, Munn A, Payne G. A novel structural model for regulation of clathrin function. EMBO J. 1997;16:2227-39 pubmed
    ..We have used random and site-directed mutagenesis of the yeast clathrin heavy chain gene (CHC1) to characterize regions which determine Chc trimerization and binding to the clathrin light ..
  15. Friant S, Pécheur E, Eugster A, Michel F, Lefkir Y, Nourrisson D, et al. Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body. Dev Cell. 2003;5:499-511 pubmed
    ..Our results show that Ent3p is required for protein sorting into intralumenal vesicles of the MVB through PtdIns(3,5)P(2) binding via its ENTH domain. ..
  16. Sakane H, Yamamoto T, Tanaka K. The functional relationship between the Cdc50p-Drs2p putative aminophospholipid translocase and the Arf GAP Gcs1p in vesicle formation in the retrieval pathway from yeast early endosomes to the TGN. Cell Struct Funct. 2006;31:87-108 pubmed
    ..These results suggest that Cdc50p-Drs2p plays an important role in the Arf1p-mediated formation of CCVs for the retrieval pathway from early endosomes to the TGN. ..
  17. Nelson K, Holmer M, Lemmon S. SCD5, a suppressor of clathrin deficiency, encodes a novel protein with a late secretory function in yeast. Mol Biol Cell. 1996;7:245-60 pubmed
    ..An interesting characteristic of the yeast clathrin heavy chain gene (CHC1) is that in some strains a CHC1 deletion is lethal, while in others it is not...
  18. Gurunathan S, David D, Gerst J. Dynamin and clathrin are required for the biogenesis of a distinct class of secretory vesicles in yeast. EMBO J. 2002;21:602-14 pubmed
    ..Here, we have found that disruptions in the genes encoding a dynamin-related protein (VPS1) or clathrin heavy chain (CHC1) abolish HDSV production, yielding LDSVs that contain all secreted cargos...
  19. Fang N, Ng A, Measday V, Mayor T. Hul5 HECT ubiquitin ligase plays a major role in the ubiquitylation and turnover of cytosolic misfolded proteins. Nat Cell Biol. 2011;13:1344-52 pubmed publisher
    ..These findings indicate that Hul5 is involved in a cytosolic protein quality control pathway that targets misfolded proteins for degradation. ..
  20. Mullins C, Bonifacino J. Structural requirements for function of yeast GGAs in vacuolar protein sorting, alpha-factor maturation, and interactions with clathrin. Mol Cell Biol. 2001;21:7981-94 pubmed
    ..Thus, these observations demonstrate that the yeast GGAs play important roles in the CPY pathway, vacuole biogenesis, and alpha-factor maturation and identify structural determinants that are critical for these functions. ..
  21. Feliciano D, Bultema J, Ambrosio A, Di Pietro S. In vivo andin vitro studies of adaptor-clathrin interaction. J Vis Exp. 2011;: pubmed publisher
    ..of HIP1R (yeast Sla2p), all known clathrin adaptors bind to the N-terminal -propeller domain of the clathrin heavy chain. Clathrin adaptors are modular proteins consisting of folded domains connected by unstructured flexible ..
  22. Farrell K, Grossman C, Di Pietro S. New Regulators of Clathrin-Mediated Endocytosis Identified in Saccharomyces cerevisiae by Systematic Quantitative Fluorescence Microscopy. Genetics. 2015;201:1061-70 pubmed publisher
    ..Conversely, Ede1 regulates the patch lifetime of Ubx3. Ubx3 likely regulates CME via the AAA-ATPase Cdc48, a ubiquitin-editing complex. Our results uncovered new components of the CME machinery that regulate this fundamental process. ..
  23. Newpher T, Idrissi F, Geli M, Lemmon S. Novel function of clathrin light chain in promoting endocytic vesicle formation. Mol Biol Cell. 2006;17:4343-52 pubmed
    ..We propose that LC binding to Sla2p positively regulates Sla2p for efficient endocytic vesicle formation...
  24. O Donnell A, Apffel A, Gardner R, Cyert M. Alpha-arrestins Aly1 and Aly2 regulate intracellular trafficking in response to nutrient signaling. Mol Biol Cell. 2010;21:3552-66 pubmed publisher
    ..This work defines a new role for arrestins in membrane trafficking and offers insight into how ?-arrestins coordinate signaling events with protein trafficking. ..
  25. Gelperin D, Weigle J, Nelson K, Roseboom P, Irie K, Matsumoto K, et al. 14-3-3 proteins: potential roles in vesicular transport and Ras signaling in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1995;92:11539-43 pubmed
    Deletion of the clathrin heavy-chain gene, CHC1, in the budding yeast Saccharomyces cerevisiae results in growth, morphological, and membrane trafficking defects, and in some strains chc1-delta is lethal...
  26. Pashkova N, Gakhar L, Winistorfer S, Sunshine A, Rich M, Dunham M, et al. The yeast Alix homolog Bro1 functions as a ubiquitin receptor for protein sorting into multivesicular endosomes. Dev Cell. 2013;25:520-33 pubmed publisher
    ..These data suggest that Bro1 and other Bro1 family members function in parallel with ESCRT-0 to recognize and sort Ub-cargoes...
  27. Gorynia S, Lorenz T, Costaguta G, Daboussi L, Cascio D, Payne G. Yeast Irc6p is a novel type of conserved clathrin coat accessory factor related to small G proteins. Mol Biol Cell. 2012;23:4416-29 pubmed publisher
    ..Together these results define Irc6p/p34 as a novel type of conserved clathrin accessory protein and founding members of a new G protein-like family. ..
  28. Ha S, Torabinejad J, DeWald D, Wenk M, Lucast L, De Camilli P, et al. The synaptojanin-like protein Inp53/Sjl3 functions with clathrin in a yeast TGN-to-endosome pathway distinct from the GGA protein-dependent pathway. Mol Biol Cell. 2003;14:1319-33 pubmed
    ..The proline-rich domain was shown to bind to two proteins, including clathrin heavy chain, Chc1p...
  29. Prosser D, Drivas T, Maldonado Baez L, Wendland B. Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin. J Cell Biol. 2011;195:657-71 pubmed publisher
    ..Thus, our results reveal the existence of a second pathway for endocytosis in yeast, which suggests similarities with the RhoA-dependent endocytic pathways of mammalian cells. ..
  30. Böttcher C, Wicky S, Schwarz H, Singer Krüger B. Sjl2p is specifically involved in early steps of endocytosis intimately linked to actin dynamics via the Ark1p/Prk1p kinases. FEBS Lett. 2006;580:633-41 pubmed
    ..Consistent with the finding that Sjl2p can bind to clathrin heavy chain in vitro, our results suggest that Sjl2p localizes to smooth endocytic vesicles that may be derived from ..
  31. Fernández Golbano I, Idrissi F, Giblin J, Grosshans B, Robles V, Grötsch H, et al. Crosstalk between PI(4,5)P₂and CK2 modulates actin polymerization during endocytic uptake. Dev Cell. 2014;30:746-58 pubmed publisher
    ..Our data demonstrate a holoenzyme-independent function of CK2 in endocytic budding and establish a robust genetic, functional, and molecular link between PI(4,5)Pâ‚‚and CK2, two masters of intracellular signaling. ..
  32. Huang K, Gullberg L, Nelson K, Stefan C, Blumer K, Lemmon S. Novel functions of clathrin light chains: clathrin heavy chain trimerization is defective in light chain-deficient yeast. J Cell Sci. 1997;110 ( Pt 7):899-910 pubmed
    ..chain-deficient cells showed phenotypes similar to those displayed by yeast that have a disruption in the clathrin heavy chain gene (CHC1)...
  33. Guiney E, Goldman A, Elias J, Cyert M. Calcineurin regulates the yeast synaptojanin Inp53/Sjl3 during membrane stress. Mol Biol Cell. 2015;26:769-85 pubmed publisher
    ..We propose that activation of Ca(2+)/calcineurin and PI(4,5)P2 signaling to regulate endocytosis is a fundamental and conserved response to excess membrane in eukaryotic cells. ..
  34. Feliciano D, Di Pietro S. SLAC, a complex between Sla1 and Las17, regulates actin polymerization during clathrin-mediated endocytosis. Mol Biol Cell. 2012;23:4256-72 pubmed publisher
    ..These results advance our understanding of the regulation of actin polymerization in endocytosis. ..
  35. Starita L, Lo R, Eng J, von Haller P, Fields S. Sites of ubiquitin attachment in Saccharomyces cerevisiae. Proteomics. 2012;12:236-40 pubmed publisher
    ..However, such peptides with GG shifts have been difficult to discover. We identify 870 unique sites of ubiquitin attachment on 438 different proteins of the yeast Saccharomyces cerevisiae. ..
  36. Duttler S, Pechmann S, Frydman J. Principles of cotranslational ubiquitination and quality control at the ribosome. Mol Cell. 2013;50:379-93 pubmed publisher
    ..We find that quality control at the ribosome is achieved through a tiered system wherein nascent polypeptides have a chance to fold before becoming accessible to ubiquitination. ..
  37. Newpher T, Smith R, Lemmon V, Lemmon S. In vivo dynamics of clathrin and its adaptor-dependent recruitment to the actin-based endocytic machinery in yeast. Dev Cell. 2005;9:87-98 pubmed
    ..In contrast, Sla2p, End3p, Pan1p, and a dynamic actin cytoskeleton are not required for clathrin assembly or exchange but are required for the mobility, maturation, and/or turnover of clathrin-containing endocytic structures. ..
  38. Kee Y, Lyon N, Huibregtse J. The Rsp5 ubiquitin ligase is coupled to and antagonized by the Ubp2 deubiquitinating enzyme. EMBO J. 2005;24:2414-24 pubmed
    ..Together, these results indicate that Rsp5 activity is modulated by being physically coupled to the Rup1/Ubp2 deubiquitinating enzyme complex, representing a novel mode of regulation for an HECT ubiquitin ligase. ..
  39. Deloche O, Schekman R. Vps10p cycles between the TGN and the late endosome via the plasma membrane in clathrin mutants. Mol Biol Cell. 2002;13:4296-307 pubmed
    ..normal trafficking of its sorting receptor, Vps10p, the steady state distribution of which is not altered in chc1 cells...
  40. Panek H, Stepp J, Engle H, Marks K, Tan P, Lemmon S, et al. Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex. EMBO J. 1997;16:4194-204 pubmed
    ..Combination of suppressor mutations with a clathrin heavy chain mutation (chc1-ts) confers no synthetic growth defects...
  41. Hung C, Duncan M. Clathrin binding by the adaptor Ent5 promotes late stages of clathrin coat maturation. Mol Biol Cell. 2016;27:1143-53 pubmed publisher
    ..These findings support a model in which Ent5 clathrin binding performs a mechanistic role in coat maturation, whereas Ent5 adaptor binding promotes cargo incorporation. ..
  42. Katta S, Chen J, Gardner J, Friederichs J, Smith S, Gogol M, et al. Sec66-Dependent Regulation of Yeast Spindle-Pole Body Duplication Through Pom152. Genetics. 2015;201:1479-95 pubmed publisher
  43. Baggett J, D Aquino K, Wendland B. The Sla2p talin domain plays a role in endocytosis in Saccharomyces cerevisiae. Genetics. 2003;165:1661-74 pubmed
    ..Together, our data support the view that Sla2p is an adaptor that links actin to clathrin and endocytosis. ..
  44. Ziv I, Matiuhin Y, Kirkpatrick D, Erpapazoglou Z, Leon S, Pantazopoulou M, et al. A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis. Mol Cell Proteomics. 2011;10:M111.009753 pubmed publisher
    ..We conclude that despite the shared use of the ubiquitin molecule, the two branches of the ubiquitin machinery--the ubiquitin-proteasome system and the ubiquitin trafficking system--were unevenly perturbed by expression of K0 ubiquitin. ..
  45. Collette J, Chi R, Boettner D, Fernandez Golbano I, Plemel R, Merz A, et al. Clathrin functions in the absence of the terminal domain binding site for adaptor-associated clathrin-box motifs. Mol Biol Cell. 2009;20:3401-13 pubmed publisher
    ..Clathrin recruitment to membranes is mediated by the clathrin heavy chain (HC) N-terminal domain (TD), which forms a seven-bladed beta-propeller...
  46. Sanchatjate S, Schekman R. Chs5/6 complex: a multiprotein complex that interacts with and conveys chitin synthase III from the trans-Golgi network to the cell surface. Mol Biol Cell. 2006;17:4157-66 pubmed
    ..Our results suggest a model of protein sorting at the TGN that involves a peripheral, possibly coat, complex that includes multiple related copies of a specificity determining subunit. ..
  47. Xiao J, Kim L, Graham T. Dissection of Swa2p/auxilin domain requirements for cochaperoning Hsp70 clathrin-uncoating activity in vivo. Mol Biol Cell. 2006;17:3281-90 pubmed
    ..These data suggest that the TPR and J-domains collaborate in a bipartite interaction with Hsp70 to regulate its activity in clathrin disassembly. ..
  48. Bilodeau P, Winistorfer S, Kearney W, Robertson A, Piper R. Vps27-Hse1 and ESCRT-I complexes cooperate to increase efficiency of sorting ubiquitinated proteins at the endosome. J Cell Biol. 2003;163:237-43 pubmed
    ..These results indicate that both Ub-sorting complexes are coupled for efficient recognition of ubiquitinated cargo. ..
  49. Chen C, Ingram M, Rosal P, Graham T. Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function. J Cell Biol. 1999;147:1223-36 pubmed
    ..The drs2 null allele is also synthetically lethal with clathrin heavy chain (chc1) temperature-sensitive alleles, but not with mutations in COPI subunits or other SEC genes tested...
  50. Nelson K, Lemmon S. Suppressors of clathrin deficiency: overexpression of ubiquitin rescues lethal strains of clathrin-deficient Saccharomyces cerevisiae. Mol Cell Biol. 1993;13:521-32 pubmed
    ..To identify the SCD1 locus and other genes that can rescue chc1 delta scd1-i cells to viability, a multicopy suppressor selection strategy was developed...
  51. Hung C, Aoh Q, Joglekar A, Payne G, Duncan M. Adaptor autoregulation promotes coordinated binding within clathrin coats. J Biol Chem. 2012;287:17398-407 pubmed publisher
    ..This autoregulation influences the temporal and/or spatial location of the Gga2-Ent5 interaction. We propose that this synergistic binding provides built-in regulation to ensure the correct assembly of clathrin coats. ..
  52. Shen D, Yuan H, Hutagalung A, Verma A, Kümmel D, Wu X, et al. The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p. J Cell Biol. 2013;202:509-26 pubmed publisher
    ..We propose that the exocyst is recruited to secretory vesicles by the combinatorial signals of Sec4-GTP and the Snc proteins. This could help to confer both specificity and directionality to vesicular traffic. ..
  53. Henry K, D hondt K, Chang J, Newpher T, Huang K, Hudson R, et al. Scd5p and clathrin function are important for cortical actin organization, endocytosis, and localization of sla2p in yeast. Mol Biol Cell. 2002;13:2607-25 pubmed
    ..Therefore, Scd5p and clathrin are important for actin organization and endocytosis, and Sla2p may provide a critical link between clathrin and the actin cytoskeleton in yeast, similar to HIP1(R) in animal cells. ..
  54. Deloche O, Yeung B, Payne G, Schekman R. Vps10p transport from the trans-Golgi network to the endosome is mediated by clathrin-coated vesicles. Mol Biol Cell. 2001;12:475-85 pubmed
    ..The Vps10p C-terminal domain appears to play a principal role in retrieval of Vps10p from the prevacuolar compartment rather than in sorting from the TGN...
  55. Boettner D, SEGARRA V, Moorthy B, de Leon N, Creagh J, Collette J, et al. Creating a chimeric clathrin heavy chain that functions independently of yeast clathrin light chain. Traffic. 2016;17:754-68 pubmed publisher
    ..in mammals, yeast clathrin function requires both the clathrin heavy (CHC) and clathrin light (CLC) chain, since Chc1 does not form stable trimers without Clc1...