CFT1

Summary

Gene Symbol: CFT1
Description: Cft1p
Alias: YHH1, Cft1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Ghazy M, He X, Singh B, Hampsey M, Moore C. The essential N terminus of the Pta1 scaffold protein is required for snoRNA transcription termination and Ssu72 function but is dispensable for pre-mRNA 3'-end processing. Mol Cell Biol. 2009;29:2296-307 pubmed publisher
    ..These findings suggest that the amino terminus of Pta1 has an inhibitory effect and that this effect can be neutralized through the interaction with Ssu72. ..
  2. Zhao J, Kessler M, Helmling S, O Connor J, Moore C. Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol. 1999;19:7733-40 pubmed
    ..The three largest subunits, Cft1/Yhh1, Cft2/Ydh1, and Brr5/Ysh1, are homologs of the three largest subunits of mammalian cleavage-polyadenylation ..
  3. Kim M, Krogan N, Vasiljeva L, Rando O, Nedea E, Greenblatt J, et al. The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II. Nature. 2004;432:517-22 pubmed
    ..These findings support a model in which poly(A) site cleavage and subsequent degradation of the 3'-downstream RNA by Rat1 trigger transcription termination. ..
  4. Ohnacker M, Barabino S, Preker P, Keller W. The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex. EMBO J. 2000;19:37-47 pubmed
    ..These results show that Pfs2p plays an essential role in 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex. ..
  5. Kyburz A, Sadowski M, Dichtl B, Keller W. The role of the yeast cleavage and polyadenylation factor subunit Ydh1p/Cft2p in pre-mRNA 3'-end formation. Nucleic Acids Res. 2003;31:3936-45 pubmed
  6. Nedea E, He X, Kim M, Pootoolal J, Zhong G, Canadien V, et al. Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem. 2003;278:33000-10 pubmed
    ..Ref2 and Pta1 similarly affect at least one snoRNA transcript. ..
  7. Gross S, Moore C. Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I. Proc Natl Acad Sci U S A. 2001;98:6080-5 pubmed
    ..Analysis of the individual protein-protein interactions supports an architectural model for CF I in which Pcf11 simultaneously interacts with Rna14, Rna15, and Clp1, whereas Rna14 bridges Rna15 and Hrp1. ..
  8. He X, Moore C. Regulation of yeast mRNA 3' end processing by phosphorylation. Mol Cell. 2005;19:619-29 pubmed
    ..These results support a model in which poly(A) synthesis is controlled by cycles of phosphorylation and dephosphorylation that require the action of Glc7. ..
  9. Dichtl B, Blank D, Sadowski M, Hubner W, Weiser S, Keller W. Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination. EMBO J. 2002;21:4125-35 pubmed
    ..We show that Yhh1p/Cft1p, the yeast homologue of the mammalian AAUAAA interacting protein CPSF 160, is an RNA-binding protein and provide ..

More Information

Publications22

  1. Zhang D, Mosley A, Ramisetty S, Rodríguez Molina J, Washburn M, Ansari A. Ssu72 phosphatase-dependent erasure of phospho-Ser7 marks on the RNA polymerase II C-terminal domain is essential for viability and transcription termination. J Biol Chem. 2012;287:8541-51 pubmed publisher
    ..An inability to remove these marks prevents Pol II from terminating efficiently and will likely impede subsequent assembly into the pre-initiation complex. ..
  2. Dichtl B, Blank D, Ohnacker M, Friedlein A, Roeder D, Langen H, et al. A role for SSU72 in balancing RNA polymerase II transcription elongation and termination. Mol Cell. 2002;10:1139-50 pubmed
    ..The sum of our analyses suggests a negative influence of Ssu72p on RNAP II during transcription that affects the commitment to either elongation or termination. ..
  3. Schreieck A, Easter A, Etzold S, Wiederhold K, Lidschreiber M, Cramer P, et al. RNA polymerase II termination involves C-terminal-domain tyrosine dephosphorylation by CPF subunit Glc7. Nat Struct Mol Biol. 2014;21:175-179 pubmed publisher
    ..These results show that transcription termination involves Tyr1 dephosphorylation of the CTD and indicate that pre-mRNA processing by CPF and transcription termination are coupled via Glc7-dependent Pol II-Tyr1 dephosphorylation. ..
  4. Dheur S, Nykamp K, Viphakone N, Swanson M, Minvielle Sebastia L. Yeast mRNA Poly(A) tail length control can be reconstituted in vitro in the absence of Pab1p-dependent Poly(A) nuclease activity. J Biol Chem. 2005;280:24532-8 pubmed
    ..Based on these findings, we propose that Nab2p is necessary and sufficient to regulate poly(A) tail length during de novo synthesis in yeast. ..
  5. Vo L, Minet M, Schmitter J, Lacroute F, Wyers F. Mpe1, a zinc knuckle protein, is an essential component of yeast cleavage and polyadenylation factor required for the cleavage and polyadenylation of mRNA. Mol Cell Biol. 2001;21:8346-56 pubmed
    ..These results show that Mpe1p plays a crucial role in 3' end formation probably by promoting the specific link between the CFI/CPF complex and pre-mRNA. ..
  6. Nordick K, Hoffman M, BETZ J, Jaehning J. Direct interactions between the Paf1 complex and a cleavage and polyadenylation factor are revealed by dissociation of Paf1 from RNA polymerase II. Eukaryot Cell. 2008;7:1158-67 pubmed publisher
    ..We found that the cleavage and polyadenylation factor Cft1 requires the Pol II-associated form of the Paf1 complex for full levels of interaction with the serine 5-..
  7. Ghazy M, Gordon J, Lee S, Singh B, Bohm A, Hampsey M, et al. The interaction of Pcf11 and Clp1 is needed for mRNA 3'-end formation and is modulated by amino acids in the ATP-binding site. Nucleic Acids Res. 2012;40:1214-25 pubmed publisher
    ..These results define Clp1 as a bridge between CF IA and CPF and indicate that the Clp1-Pcf11 interaction is modulated by amino acids in the conserved ATP-binding site of Clp1. ..
  8. Zhao J, Kessler M, Moore C. Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA. J Biol Chem. 1997;272:10831-8 pubmed
    ..The 150-kDa protein is recognized by antibodies against Cft1, the yeast homologue of the 160-kDa subunit of the mammalian cleavage/polyadenylation specificity factor (CPSF)...
  9. Ezeokonkwo C, Ghazy M, Zhelkovsky A, Yeh P, Moore C. Novel interactions at the essential N-terminus of poly(A) polymerase that could regulate poly(A) addition in Saccharomyces cerevisiae. FEBS Lett. 2012;586:1173-8 pubmed publisher
    ..We have now identified proteins that interact specifically with this region. Cft1 and Pta1 are subunits of the cleavage/polyadenylation factor, in which Pap1 resides, and Nab6 and Sub1 are nucleic-..
  10. Lee S, Moore C. Efficient mRNA polyadenylation requires a ubiquitin-like domain, a zinc knuckle, and a RING finger domain, all contained in the Mpe1 protein. Mol Cell Biol. 2014;34:3955-67 pubmed publisher
    ..Furthermore, an inhibitor of ubiquitin-mediated interactions blocks cleavage, demonstrating for the first time a direct role for ubiquitination in mRNA 3'-end processing. ..
  11. Preker P, Ohnacker M, Minvielle Sebastia L, Keller W. A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor. EMBO J. 1997;16:4727-37 pubmed
    ..PF I also appears to be functionally related to CPSF, as it polyadenylates a substrate RNA more efficiently than Pap1p alone. Possibly, the observed interaction of the complex with RNA tethers Pap1p to its substrate. ..
  12. Ezeokonkwo C, Zhelkovsky A, Lee R, Bohm A, Moore C. A flexible linker region in Fip1 is needed for efficient mRNA polyadenylation. RNA. 2011;17:652-64 pubmed publisher
    ..Our results indicate that the Fip1 linker, through its flexibility and protein/protein interactions, allows Pap1 to reach the 3' end of the cleaved RNA and efficiently initiate poly(A) addition. ..
  13. Roguev A, Schaft D, Shevchenko A, Pijnappel W, Wilm M, Aasland R, et al. The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4. EMBO J. 2001;20:7137-48 pubmed
    ..We propose that eukaryotic Set1Cs are H3 lysine 4 methyltransferases and are related to trxG action through association with Ash2 homologues. ..