Gene Symbol: CFD1
Description: iron-sulfur cluster assembly protein CFD1
Alias: DRE3, iron-sulfur cluster assembly protein CFD1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Netz D, Stümpfig M, Dore C, Muhlenhoff U, Pierik A, Lill R. Tah18 transfers electrons to Dre2 in cytosolic iron-sulfur protein biogenesis. Nat Chem Biol. 2010;6:758-65 pubmed publisher
    ..The pathway is conserved in eukaryotes, as human Ndor1-Ciapin1 proteins can functionally replace yeast Tah18-Dre2. ..
  2. Roy A, Solodovnikova N, Nicholson T, Antholine W, Walden W. A novel eukaryotic factor for cytosolic Fe-S cluster assembly. EMBO J. 2003;22:4826-35 pubmed
    ..of IRP1 to c-aconitase led to the identification of a previously uncharacterized, essential gene, which we call CFD1 (cytosolic Fe-S cluster deficient). CFD1 encodes a highly conserved, putative P-loop ATPase...
  3. Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher
    ..Like the absence of the mcm(5) side chain, the lack of the s(2) group renders tRNA(mcm5s2UUC Glu) less sensitive to gamma-toxin, reinforcing the importance of the wobble nucleoside mcm(5)s(2)U for tRNA cleavage by gamma-toxin. ..
  4. Netz D, Pierik A, Stümpfig M, Muhlenhoff U, Lill R. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. Nat Chem Biol. 2007;3:278-86 pubmed
    ..So far, four highly conserved proteins (Cfd1, Nbp35, Nar1 and Cia1) have been identified as members of the cytosolic [Fe-S] protein assembly machinery, but ..
  5. Netz D, Pierik A, Stümpfig M, Bill E, Sharma A, Pallesen L, et al. A bridging [4Fe-4S] cluster and nucleotide binding are essential for function of the Cfd1-Nbp35 complex as a scaffold in iron-sulfur protein maturation. J Biol Chem. 2012;287:12365-78 pubmed publisher
    The essential P-loop NTPases Cfd1 and Nbp35 of the cytosolic iron-sulfur (Fe-S) protein assembly machinery perform a scaffold function for Fe-S cluster synthesis...
  6. Yarunin A, Panse V, Petfalski E, Dez C, Tollervey D, Hurt E. Functional link between ribosome formation and biogenesis of iron-sulfur proteins. EMBO J. 2005;24:580-8 pubmed
    In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis...
  7. Zhang Y, Lyver E, Nakamaru Ogiso E, Yoon H, Amutha B, Lee D, et al. Dre2, a conserved eukaryotic Fe/S cluster protein, functions in cytosolic Fe/S protein biogenesis. Mol Cell Biol. 2008;28:5569-82 pubmed publisher
  8. Balk J, Aguilar Netz D, Tepper K, Pierik A, Lill R. The essential WD40 protein Cia1 is involved in a late step of cytosolic and nuclear iron-sulfur protein assembly. Mol Cell Biol. 2005;25:10833-41 pubmed
    ..export machineries in mitochondria and three recently identified extramitochondrial proteins, the P-loop NTPases Cfd1 and Nbp35 and the hydrogenase-like Nar1...
  9. Camire E, Grossman J, Thole G, Fleischman N, Perlstein D. The Yeast Nbp35-Cfd1 Cytosolic Iron-Sulfur Cluster Scaffold Is an ATPase. J Biol Chem. 2015;290:23793-802 pubmed publisher
    Nbp35 and Cfd1 are prototypical members of the MRP/Nbp35 class of iron-sulfur (FeS) cluster scaffolds that function to assemble nascent FeS clusters for transfer to FeS-requiring enzymes...

More Information


  1. Hausmann A, Aguilar Netz D, Balk J, Pierik A, Muhlenhoff U, Lill R. The eukaryotic P loop NTPase Nbp35: an essential component of the cytosolic and nuclear iron-sulfur protein assembly machinery. Proc Natl Acad Sci U S A. 2005;102:3266-71 pubmed
    ..The findings provide strong evidence for the existence of a highly conserved and essential machinery dedicated to assembling cytosolic and nuclear Fe/S proteins. ..
  2. Pallesen L, Solodovnikova N, Sharma A, Walden W. Interaction with Cfd1 increases the kinetic lability of FeS on the Nbp35 scaffold. J Biol Chem. 2013;288:23358-67 pubmed publisher
    ..In yeast and animals, Cfd1 and Nbp35 are homologous P-loop NTPases that form a heterotetrameric complex essential for FeS protein maturation ..