Gene Symbol: CET1
Description: polynucleotide 5'-phosphatase
Alias: CES5, polynucleotide 5'-phosphatase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Cho E, Rodriguez C, Takagi T, Buratowski S. Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain. Genes Dev. 1998;12:3482-7 pubmed
    ..In the yeast Saccharomyces cerevisiae, capping enzyme is composed of two subunits, the mRNA 5'-triphosphatase (Cet1) and the mRNA guanylyltransferase (Ceg1). Here we map interactions between Ceg1, Cet1, and the CTD...
  2. Gu M, Rajashankar K, Lima C. Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatus. Structure. 2010;18:216-27 pubmed publisher
    ..In Saccharomyces cerevisiae, the first two steps in capping are catalyzed by the RNA triphosphatase Cet1 and RNA guanylyltransferase Ceg1, which form a complex that is directly recruited to phosphorylated RNA polymerase ..
  3. Ho C, Lehman K, Shuman S. An essential surface motif (WAQKW) of yeast RNA triphosphatase mediates formation of the mRNA capping enzyme complex with RNA guanylyltransferase. Nucleic Acids Res. 1999;27:4671-8 pubmed
    ..Trypsin-sensitive sites of Ceg1p that are shielded from proteolysis when Ceg1p is bound to Cet1p are located between nucleotidyl transferase motifs V and VI. ..
  4. Hausmann S, Ho C, Schwer B, Shuman S. An essential function of Saccharomyces cerevisiae RNA triphosphatase Cet1 is to stabilize RNA guanylyltransferase Ceg1 against thermal inactivation. J Biol Chem. 2001;276:36116-24 pubmed
    Saccharomyces cerevisiae RNA triphosphatase (Cet1) and RNA guanylyltransferase (Ceg1) interact in vivo and in vitro to form a bifunctional mRNA capping enzyme complex...
  5. Lehman K, Schwer B, Ho C, Rouzankina I, Shuman S. A conserved domain of yeast RNA triphosphatase flanking the catalytic core regulates self-association and interaction with the guanylyltransferase component of the mRNA capping apparatus. J Biol Chem. 1999;274:22668-78 pubmed
    ..Sedimentation analysis indicates that purified Cet1(231-549)p is a homodimer. Cet1(231-549)p binds in vitro to the yeast RNA guanylyltransferase Ceg1p to form a 7...
  6. Takase Y, Takagi T, Komarnitsky P, Buratowski S. The essential interaction between yeast mRNA capping enzyme subunits is not required for triphosphatase function in vivo. Mol Cell Biol. 2000;20:9307-16 pubmed
    The Saccharomyces cerevisiae mRNA capping enzyme consists of two subunits: an RNA 5'-triphosphatase (Cet1) and an mRNA guanylyltransferase (Ceg1)...
  7. Tsukamoto T, Shibagaki Y, Imajoh Ohmi S, Murakoshi T, Suzuki M, Nakamura A, et al. Isolation and characterization of the yeast mRNA capping enzyme beta subunit gene encoding RNA 5'-triphosphatase, which is essential for cell viability. Biochem Biophys Res Commun. 1997;239:116-22 pubmed
    ..with the aid of partial amino acid sequences of purified yeast capping enzyme, we isolated the gene, designated CET1, encoding the S. cerevisiae capping enzyme beta subunit...
  8. Ho C, Schwer B, Shuman S. Genetic, physical, and functional interactions between the triphosphatase and guanylyltransferase components of the yeast mRNA capping apparatus. Mol Cell Biol. 1998;18:5189-98 pubmed
    We have characterized an essential Saccharomyces cerevisiae gene, CES5, that when present in high copy, suppresses the temperature-sensitive growth defect caused by the ceg1-25 mutation of the yeast mRNA guanylyltransferase (capping ..
  9. Lima C, Wang L, Shuman S. Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus. Cell. 1999;99:533-43 pubmed
    ..The structure and mechanism of fungal RNA triphosphatases are completely different from those of mammalian mRNA capping enzymes. Hence, RNA triphosphatase presents an ideal target for structure-based antifungal drug discovery. ..

More Information


  1. Lahudkar S, Durairaj G, Uprety B, Bhaumik S. A novel role for Cet1p mRNA 5'-triphosphatase in promoter proximal accumulation of RNA polymerase II in Saccharomyces cerevisiase. Genetics. 2014;196:161-76 pubmed publisher
    ..Collectively, our results demonstrate a novel role of Cet1p in regulation of promoter proximal accumulation of RNA polymerase II independently of mRNA capping activity, and hence transcription in vivo...
  2. Sung M, Huh W. In vivo quantification of protein-protein interactions in Saccharomyces cerevisiae using bimolecular fluorescence complementation assay. J Microbiol Methods. 2010;83:194-201 pubmed publisher
  3. Mukai Y, Davie J, Dent S. Physical and functional interaction of the yeast corepressor Tup1 with mRNA 5'-triphosphatase. J Biol Chem. 2003;278:18895-901 pubmed
    ..Similarly, the S. cerevisiae Tup1 protein associates with the mRNA 5'-triphosphatase encoded by the CET1 gene...
  4. Krogan N, Dover J, Wood A, Schneider J, Heidt J, Boateng M, et al. The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation. Mol Cell. 2003;11:721-9 pubmed
    ..Collectively, our results suggest that the Paf1 complex is required for histone H3 methylation, therefore linking transcriptional elongation to chromatin methylation. ..
  5. Sen R, Kaja A, Ferdoush J, Lahudkar S, Barman P, Bhaumik S. An mRNA Capping Enzyme Targets FACT to the Active Gene To Enhance the Engagement of RNA Polymerase II into Transcriptional Elongation. Mol Cell Biol. 2017;37: pubmed publisher
    We have recently demonstrated that an mRNA capping enzyme, Cet1, impairs promoter-proximal accumulation/pausing of RNA polymerase II (Pol II) independently of its capping activity in Saccharomyces cerevisiae to control ..
  6. Suh M, Meyer P, Gu M, Ye P, Zhang M, Kaplan C, et al. A dual interface determines the recognition of RNA polymerase II by RNA capping enzyme. J Biol Chem. 2010;285:34027-38 pubmed publisher
    ..Our results indicate that the dual interface based on combining PCI1 and PCI2 is required for directing CE to Pol II elongation complexes. ..
  7. Yamada Okabe T, Mio T, Matsui M, Kashima Y, Arisawa M, Yamada Okabe H. Isolation and characterization of the Candida albicans gene for mRNA 5'-triphosphatase: association of mRNA 5'-triphosphatase and mRNA 5'-guanylyltransferase activities is essential for the function of mRNA 5'-capping enzyme in vivo. FEBS Lett. 1998;435:49-54 pubmed
    ..CaCET1 rescued CET1-deficient S...
  8. Cho E, Takagi T, Moore C, Buratowski S. mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain. Genes Dev. 1997;11:3319-26 pubmed
    ..Our results provide in vitro and in vivo evidence that capping enzyme is recruited to the transcription complex via phosphorylation of the RNA polymerase CTD. ..
  9. Myers L, Lacomis L, Erdjument Bromage H, Tempst P. The yeast capping enzyme represses RNA polymerase II transcription. Mol Cell. 2002;10:883-94 pubmed
    ..Efficient pre-mRNA capping relies on interactions between the capping enzyme and transcription apparatus. Repression by the capping enzyme suggests a bidirectional flow of information between capping and transcription. ..
  10. Takizawa N, Fujiwara T, Yamasaki M, Saito A, Fukao A, Nomoto A, et al. The essential role for the RNA triphosphatase Cet1p in nuclear import of the mRNA capping enzyme Cet1p-Ceg1p complex of Saccharomyces cerevisiae. PLoS ONE. 2013;8:e78000 pubmed publisher
    ..The results indicate that the Cet1p-Ceg1p interaction is important not only for the activation of Ceg1p, but also for nuclear import of the complex. ..
  11. Xu Y, Triantafyllou I, Cable M, Palermo R. High-throughput assays for yeast RNA 5' triphosphatase (Cet1p). Anal Biochem. 2008;372:89-95 pubmed
    ..The charcoal absorption method run in the 96-well plate format has general utility for any phosphatase using nucleotides, nucleic acids, or proteins as substrate. ..
  12. Lidschreiber M, Leike K, Cramer P. Cap completion and C-terminal repeat domain kinase recruitment underlie the initiation-elongation transition of RNA polymerase II. Mol Cell Biol. 2013;33:3805-16 pubmed publisher
    ..We show that the early Pol II elongation factor Spt5 contributes to stable recruitment of the mRNA capping enzymes Cet1, Ceg1, and Abd1...
  13. Vincent K, Wang Q, Jay S, Hobbs K, Rymond B. Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing. Genetics. 2003;164:895-907 pubmed
    ..Prp19, Prp22, and Syf2 splicing factors and four proteins without established contribution to splicing (Bud13, Cet1, Cwc2, and Rds3)...
  14. Muratani M, Kung C, Shokat K, Tansey W. The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing. Cell. 2005;120:887-99 pubmed
    ..We propose that Gal4 ubiquitylation and destruction are required for initiation-competent transcription complexes to transition to fully mature elongating complexes capable of appropriate mRNA processing. ..