Gene Symbol: CEF1
Description: Cef1p
Alias: NTC85, Cef1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Chen C, Tsai W, Chen H, Wang C, Cheng S. Identification and characterization of two novel components of the Prp19p-associated complex, Ntc30p and Ntc20p. J Biol Chem. 2001;276:488-94 pubmed
    ..These results indicate that Ntc30p and Ntc20p are auxiliary splicing factors the functions of which may be modulating the function of the Prp19p-associated complex. ..
  2. Ben Yehuda S, Dix I, Russell C, McGarvey M, Beggs J, Kupiec M. Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae. Genetics. 2000;156:1503-17 pubmed
    ..and biochemical analysis, we show that the SYF gene products interact with each other and with four other proteins: Isy1p, Cef1p, Prp22p, and Ntc20p. We discuss the role played by these proteins in splicing and cell cycle progression.
  3. Burns C, Ohi R, Mehta S, O TOOLE E, Winey M, Clark T, et al. Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:801-15 pubmed
    ..Among these are the conserved, Myb-related CDC5 (also known as Cef1p in S. cerevisiae) proteins...
  4. Chen C, Yu W, Tsao T, Wang L, Chen H, Lin J, et al. Functional and physical interactions between components of the Prp19p-associated complex. Nucleic Acids Res. 2002;30:1029-37 pubmed
    ..Genetic analysis suggests that Ntc31p, Ntc30p and Ntc20p may play roles in modulating the function of Ntc90p. ..
  5. Chang K, Chen H, Cheng S. Ntc90 is required for recruiting first step factor Yju2 but not for spliceosome activation. RNA. 2009;15:1729-39 pubmed publisher
    ..Ntc90 is not required for the association of Prp19, Ntc85, Ntc77, Ntc25, and Ntc20, or for their binding to the spliceosome...
  6. Liu Y, Chen H, Wu N, Cheng S. A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing. Mol Cell Biol. 2007;27:5403-13 pubmed
  7. Yeh T, Liu H, Chung C, Wu N, Liu Y, Cheng S. Splicing factor Cwc22 is required for the function of Prp2 and for the spliceosome to escape from a futile pathway. Mol Cell Biol. 2011;31:43-53 pubmed publisher
    Cwc22 was previously identified to associate with the pre-mRNA splicing factor Cef1/Ntc85, a component of the Prp19-associated complex (nineteen complex [NTC]) involved in spliceosome activation...
  8. Tsai W, Chow Y, Chen H, Huang K, Hong R, Jan S, et al. Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing. J Biol Chem. 1999;274:9455-62 pubmed
    ..By sequencing components of the affinity-purified complex, we have identified Cef1p as a component of the Prp19p-associated complex, Ntc85p...
  9. Chiu Y, Liu Y, Chiang T, Yeh T, Tseng C, Wu N, et al. Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction. Mol Cell Biol. 2009;29:5671-8 pubmed publisher
    Cwc25 has previously been identified to associate with pre-mRNA splicing factor Cef1/Ntc85, a component of the Prp19-associated complex (nineteen complex, or NTC) involved in spliceosome activation...

More Information


  1. Gahura O, Abrhámová K, Skruzny M, Valentová A, Munzarová V, Folk P, et al. Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates. J Cell Biochem. 2009;106:139-51 pubmed publisher
    ..screen, we found that prp45(1-169) genetically interacts with alleles of NTC members SYF1, CLF1/SYF3, NTC20, and CEF1, and 2nd step splicing factors SLU7, PRP17, PRP18, and PRP22...
  2. Chen H, Chang K, Su Y, Huang Y, Cheng S. Structural requirement of Ntc77 for spliceosome activation and first catalytic step. Nucleic Acids Res. 2014;42:12261-71 pubmed publisher
    ..Our data reveal functional roles of Ntc77 in both spliceosome activation and the first catalytic step, and distinct structural domains of Ntc77 required for these two steps. ..
  3. Query C, Konarska M. CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. RNA. 2012;18:1001-13 pubmed publisher
    ..We have now identified the strongest non-prp8 suppressors as alleles of the NTC (Prp19 complex) component, CEF1. These cef1 alleles generally suppress second-step defects caused by a variety of intron mutations, mutations in U6 ..
  4. Hoskins A, Friedman L, Gallagher S, Crawford D, Anderson E, Wombacher R, et al. Ordered and dynamic assembly of single spliceosomes. Science. 2011;331:1289-95 pubmed publisher
    ..This experimental strategy should prove widely useful for mechanistic analysis of other macromolecular machines in environments approaching the complexity of living cells. ..
  5. Goldfeder M, Oliveira C. Cwc24p, a novel Saccharomyces cerevisiae nuclear ring finger protein, affects pre-snoRNA U3 splicing. J Biol Chem. 2008;283:2644-53 pubmed
    ..Cwc24p, a novel yeast RING finger protein that had been previously isolated in a complex with the splicing factor Cef1p. Here we show that, consistent with the protein interaction data, Cwc24p localizes to the cell nucleus, and its ..
  6. Tsai R, Fu R, Yeh F, Tseng C, Lin Y, Huang Y, et al. Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2. Genes Dev. 2005;19:2991-3003 pubmed
    ..This is the first demonstration of physical disassembly of the spliceosome, catalyzed by a complex containing a DExD/H-box RNA helicase and two accessory factors, which might function in targeting the helicase to the correct substrate. ..
  7. Chanarat S, Seizl M, Strasser K. The Prp19 complex is a novel transcription elongation factor required for TREX occupancy at transcribed genes. Genes Dev. 2011;25:1147-58 pubmed publisher
  8. Ohi M, Vander Kooi C, Rosenberg J, Ren L, Hirsch J, Chazin W, et al. Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Mol Cell Biol. 2005;25:451-60 pubmed
    ..consistent with Prp19p tetramerization providing an interaction surface for a single copy of its binding partner, Cef1p. Electron microscopy showed that the isolated Prp19p tetramer is an elongated particle consisting of four globular ..
  9. Ohi M, Gould K. Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. RNA. 2002;8:798-815 pubmed
    Schizosaccharomyces pombe (Sp) Cdc5p and its Saccharomyces cerevisiae (Sc) ortholog, Cef1p, are essential components of the spliceosome. In S...
  10. Hoskins A, Rodgers M, Friedman L, Gelles J, Moore M. Single molecule analysis reveals reversible and irreversible steps during spliceosome activation. elife. 2016;5: pubmed publisher
    ..The data reveal the activation mechanism and show that overall splicing efficiency may be maintained through repeated rounds of disassembly and tri-snRNP reassociation. ..
  11. Bai R, Yan C, Wan R, Lei J, Shi Y. Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae. Cell. 2017;171:1589-1598.e8 pubmed publisher
    ..The 3'SS is stabilized through an interaction with the 1585-loop of Prp8. The P complex structure provides a view on splice junction formation critical for understanding the complete splicing cycle. ..