CDC7

Summary

Gene Symbol: CDC7
Description: serine/threonine protein kinase CDC7
Alias: LSD6, SAS1, serine/threonine protein kinase CDC7
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kitada K, Johnston L, Sugino T, Sugino A. Temperature-sensitive cdc7 mutations of Saccharomyces cerevisiae are suppressed by the DBF4 gene, which is required for the G1/S cell cycle transition. Genetics. 1992;131:21-9 pubmed
    ..on a multicopy plasmid, a gene from a Saccharomyces cerevisiae genomic library suppresses the temperature-sensitive cdc7-1 mutation...
  2. Devault A, Gueydon E, Schwob E. Interplay between S-cyclin-dependent kinase and Dbf4-dependent kinase in controlling DNA replication through phosphorylation of yeast Mcm4 N-terminal domain. Mol Biol Cell. 2008;19:2267-77 pubmed publisher
    ..We propose that the N-terminal extension of eukaryotic Mcm4 integrates regulatory inputs from S-CDK and DDK, which may play an important role for the proper assembly or stabilization of replisome-progression complexes. ..
  3. Harkins V, Gabrielse C, Haste L, Weinreich M. Budding yeast Dbf4 sequences required for Cdc7 kinase activation and identification of a functional relationship between the Dbf4 and Rev1 BRCT domains. Genetics. 2009;183:1269-82 pubmed publisher
    b>Cdc7-Dbf4 is a two-subunit kinase required for initiating DNA replication. The Dbf4 regulatory subunit is required for Cdc7 kinase activity...
  4. Gabrielse C, Miller C, McConnell K, DeWard A, Fox C, Weinreich M. A Dbf4p BRCA1 C-terminal-like domain required for the response to replication fork arrest in budding yeast. Genetics. 2006;173:541-55 pubmed
    ..Rad53p likely directly phosphorylated Dbf4p in response to RF arrest and Dbf4p was required for Rad53p abundance. Rad53p and Dbf4p therefore cooperated to coordinate a robust cellular response to RF arrest. ..
  5. Wan L, Niu H, Futcher B, Zhang C, Shokat K, Boulton S, et al. Cdc28-Clb5 (CDK-S) and Cdc7-Dbf4 (DDK) collaborate to initiate meiotic recombination in yeast. Genes Dev. 2008;22:386-97 pubmed publisher
    S-phase cyclin-dependent kinase Cdc28-Clb5 (CDK-S) and Dbf4-dependent kinase Cdc7-Dbf4 (DDK) are highly conserved kinases well known for their roles in the initiation of DNA replication...
  6. On K, Beuron F, Frith D, Snijders A, Morris E, Diffley J. Prereplicative complexes assembled in vitro support origin-dependent and independent DNA replication. EMBO J. 2014;33:605-20 pubmed publisher
    ..These experiments indicate that Mcm2-7 double hexamers can be precursors of replication and provide insight into the nature of eukaryotic DNA replication origins. ..
  7. Sheu Y, Stillman B. Cdc7-Dbf4 phosphorylates MCM proteins via a docking site-mediated mechanism to promote S phase progression. Mol Cell. 2006;24:101-13 pubmed
    ..We report that Cdc7-Dbf4 protein kinase (DDK) promotes assembly of a stable Cdc45-MCM complex exclusively on chromatin in S phase...
  8. Dohrmann P, Oshiro G, Tecklenburg M, Sclafani R. RAD53 regulates DBF4 independently of checkpoint function in Saccharomyces cerevisiae. Genetics. 1999;151:965-77 pubmed
    ..A genetic screen for mutations that are lethal in combination with cdc7-1 led to the isolation of seven lsd (lethal with seven defect) complementation groups...
  9. Miller C, Gabrielse C, Chen Y, Weinreich M. Cdc7p-Dbf4p regulates mitotic exit by inhibiting Polo kinase. PLoS Genet. 2009;5:e1000498 pubmed publisher
    ..Therefore, Cdc7p-Dbf4p prevents inappropriate exit from mitosis by inhibiting Polo kinase and functions in the spindle position checkpoint. ..

More Information

Publications75

  1. Hiraga S, Alvino G, Chang F, Lian H, Sridhar A, Kubota T, et al. Rif1 controls DNA replication by directing Protein Phosphatase 1 to reverse Cdc7-mediated phosphorylation of the MCM complex. Genes Dev. 2014;28:372-83 pubmed publisher
    ..eukaryotic DNA replication requires phosphorylation of the MCM complex by Dbf4-dependent kinase (DDK), composed of Cdc7 kinase and its activator, Dbf4...
  2. Varrin A, Prasad A, Scholz R, Ramer M, Duncker B. A mutation in Dbf4 motif M impairs interactions with DNA replication factors and confers increased resistance to genotoxic agents. Mol Cell Biol. 2005;25:7494-504 pubmed
    Dbf4/Cdc7 is required for DNA replication in Saccharomyces cerevisiae and appears to be a target in the S-phase checkpoint...
  3. Valentin G, Schwob E, Della Seta F. Dual role of the Cdc7-regulatory protein Dbf4 during yeast meiosis. J Biol Chem. 2006;281:2828-34 pubmed
    The Dbf4-dependent Cdc7 kinase (DDK) is essential for chromosome duplication in all eukaryotes, but was proposed to be dispensable for yeast pre-meiotic DNA replication...
  4. Dohrmann P, Sclafani R. Novel role for checkpoint Rad53 protein kinase in the initiation of chromosomal DNA replication in Saccharomyces cerevisiae. Genetics. 2006;174:87-99 pubmed
    ..Rad53 kinase is part of a signal transduction pathway involved in the DNA damage and replication checkpoints, while Cdc7-Dbf4 kinase (DDK) is important for the initiation of DNA replication...
  5. Francis L, Randell J, Takara T, Uchima L, Bell S. Incorporation into the prereplicative complex activates the Mcm2-7 helicase for Cdc7-Dbf4 phosphorylation. Genes Dev. 2009;23:643-54 pubmed publisher
    The essential S-phase kinase Cdc7-Dbf4 acts at eukaryotic origins of replication to trigger a cascade of protein associations that activate the Mcm2-7 replicative helicase...
  6. Donaldson A, Fangman W, Brewer B. Cdc7 is required throughout the yeast S phase to activate replication origins. Genes Dev. 1998;12:491-501 pubmed
    The long-standing conclusion that the Cdc7 kinase of Saccharomyces cerevisiae is required only to trigger S phase has been challenged by recent data that suggests it acts directly on individual replication origins...
  7. Hardy C, Dryga O, Seematter S, Pahl P, Sclafani R. mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p. Proc Natl Acad Sci U S A. 1997;94:3151-5 pubmed
    ..Moreover, Mcm5p/Cdc46p may have both positive and negative effects on the ability of cell to initiate replication. ..
  8. Jones D, Prasad A, Chan P, Duncker B. The Dbf4 motif C zinc finger promotes DNA replication and mediates resistance to genotoxic stress. Cell Cycle. 2010;9:2018-26 pubmed
    The Dbf4/Cdc7 kinase (DDK) plays an essential role in stimulating DNA replication by phosphorylating subunits of the Mcm2-7 helicase complex at origins...
  9. Jackson A, Pahl P, Harrison K, Rosamond J, Sclafani R. Cell cycle regulation of the yeast Cdc7 protein kinase by association with the Dbf4 protein. Mol Cell Biol. 1993;13:2899-908 pubmed
    Yeast Cdc7 protein kinase and Dbf4 protein are both required for the initiation of DNA replication at the G1/S phase boundary of the mitotic cell cycle...
  10. Bruck I, Kaplan D. Dbf4-Cdc7 phosphorylation of Mcm2 is required for cell growth. J Biol Chem. 2009;284:28823-31 pubmed publisher
    The Dbf4-Cdc7 kinase (DDK) is required for the activation of the origins of replication, and DDK phosphorylates Mcm2 in vitro. We find that budding yeast Cdc7 alone exists in solution as a weakly active multimer...
  11. Ohtoshi A, Miyake T, Arai K, Masai H. Analyses of Saccharomyces cerevisiae Cdc7 kinase point mutants: dominant-negative inhibition of DNA replication on overexpression of kinase-negative Cdc7 proteins. Mol Gen Genet. 1997;254:562-70 pubmed
    Saccharomyces cerevisiae Cdc7 kinase is required for initiation of S phase, and its kinase activity, which is positively regulated by Dbf4 protein, reaches maximum at the G1/S boundary...
  12. Weinreich M, Stillman B. Cdc7p-Dbf4p kinase binds to chromatin during S phase and is regulated by both the APC and the RAD53 checkpoint pathway. EMBO J. 1999;18:5334-46 pubmed
    ..Cdc7p may also play a role in adapting from the checkpoint response since deletion of CDC7 results in HU hypersensitivity...
  13. Kitada K, Johnson A, Johnston L, Sugino A. A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5. Mol Cell Biol. 1993;13:4445-57 pubmed
    ..required for the initiation of chromosomal DNA replication in Saccharomyces cerevisiae and that interacts with the CDC7 protein kinase...
  14. Sheu Y, Stillman B. The Dbf4-Cdc7 kinase promotes S phase by alleviating an inhibitory activity in Mcm4. Nature. 2010;463:113-7 pubmed publisher
    ..cycle-regulated protein kinase systems, the S-phase-specific cyclin-dependent protein kinases (S-CDKs) and the Dbf4-Cdc7 kinase (DDK, Dbf4-dependent protein kinase) are essential activators for initiation of DNA replication...
  15. Pessoa Brandao L, Sclafani R. CDC7/DBF4 functions in the translesion synthesis branch of the RAD6 epistasis group in Saccharomyces cerevisiae. Genetics. 2004;167:1597-610 pubmed
    b>CDC7 and DBF4 encode the essential Cdc7-Dbf4 protein kinase required for DNA replication in eukaryotes from yeast to human...
  16. Randell J, Fan A, Chan C, Francis L, Heller R, Galani K, et al. Mec1 is one of multiple kinases that prime the Mcm2-7 helicase for phosphorylation by Cdc7. Mol Cell. 2010;40:353-63 pubmed publisher
    Activation of the eukaryotic replicative DNA helicase, the Mcm2-7 complex, requires phosphorylation by Cdc7/Dbf4 (Dbf4-dependent kinase or DDK), which, in turn, depends on prior phosphorylation of Mcm2-7 by an unknown kinase (or kinases)...
  17. Hoang M, Leon R, Pessoa Brandao L, Hunt S, Raghuraman M, Fangman W, et al. Structural changes in Mcm5 protein bypass Cdc7-Dbf4 function and reduce replication origin efficiency in Saccharomyces cerevisiae. Mol Cell Biol. 2007;27:7594-602 pubmed
    ..in G(1) phase, and yet only a subset of complexes is activated during S phase by DDK (for Dbf4-dependent kinase) (Cdc7-Dbf4)...
  18. Chen Y, Weinreich M. Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction. J Biol Chem. 2010;285:41244-54 pubmed publisher
    b>Cdc7-Dbf4 is a conserved, two-subunit kinase required for initiating eukaryotic DNA replication...
  19. Ogi H, Wang C, Nakai W, Kawasaki Y, Masumoto H. The role of the Saccharomyces cerevisiae Cdc7-Dbf4 complex in the replication checkpoint. Gene. 2008;414:32-40 pubmed publisher
    The Cdc7-Dbf4 complex is a conserved serine/threonine protein kinase essential for the initiation of eukaryotic DNA replication...
  20. Lo H, Wan L, Rosebrock A, Futcher B, Hollingsworth N. Cdc7-Dbf4 regulates NDT80 transcription as well as reductional segregation during budding yeast meiosis. Mol Biol Cell. 2008;19:4956-67 pubmed publisher
    In budding yeast, as in other eukaryotes, the Cdc7 protein kinase is important for initiation of DNA synthesis in vegetative cells...
  21. Kihara M, Nakai W, Asano S, Suzuki A, Kitada K, Kawasaki Y, et al. Characterization of the yeast Cdc7p/Dbf4p complex purified from insect cells. Its protein kinase activity is regulated by Rad53p. J Biol Chem. 2000;275:35051-62 pubmed
    ..Mutant Cdc7p/Dbf4p complexes containing either Cdc7-1p or Dbf4-1 approximately 5p were also partially purified from insect cells and characterized in vitro...
  22. Oshiro G, Owens J, Shellman Y, Sclafani R, Li J. Cell cycle control of Cdc7p kinase activity through regulation of Dbf4p stability. Mol Cell Biol. 1999;19:4888-96 pubmed
    ..Thus, the regulation of Dbf4p levels through the control of Dbf4p degradation has an important role in the regulation of Cdc7p kinase activity during the cell cycle. ..
  23. Lei M, Kawasaki Y, Young M, Kihara M, Sugino A, Tye B. Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis. Genes Dev. 1997;11:3365-74 pubmed
    ..In this study, we identified DBF4, which encodes the regulatory subunit of a Cdk-like protein kinase Cdc7-Dbf4, in a screen for second site suppressors of mcm2-1...
  24. Hardy C, Pautz A. A novel role for Cdc5p in DNA replication. Mol Cell Biol. 1996;16:6775-82 pubmed
    ..cdc5-1 orc2-1 double mutants are synthetically lethal. Levels of Cdc5p were found to be cell cycle regulated and peaked in G2/M. These results suggest a role for Cdc5p and possibly Polo-like kinases at origin complexes. ..
  25. Katis V, Lipp J, Imre R, Bogdanova A, Okaz E, Habermann B, et al. Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis. Dev Cell. 2010;18:397-409 pubmed publisher
    ..within Rec8 as well as two different kinases, casein kinase 1delta/epsilon (CK1delta/epsilon) and Dbf4-dependent Cdc7 kinase (DDK), are required for Rec8 cleavage and meiosis I nuclear division...
  26. Heller R, Kang S, Lam W, Chen S, Chan C, Bell S. Eukaryotic origin-dependent DNA replication in vitro reveals sequential action of DDK and S-CDK kinases. Cell. 2011;146:80-91 pubmed publisher
    ..After helicase loading, we found that the Dbf4-dependent Cdc7 kinase (DDK) but not S phase cyclin-dependent kinase (S-CDK) is required for the initial origin recruitment of Sld3 ..
  27. Hanna J, Kroll E, Lundblad V, Spencer F. Saccharomyces cerevisiae CTF18 and CTF4 are required for sister chromatid cohesion. Mol Cell Biol. 2001;21:3144-58 pubmed
    ..The requirement for CTF4 and CTF18 in robust cohesion identifies novel roles for replication accessory proteins in this process. ..
  28. Bastia D, Srivastava P, Zaman S, Choudhury M, Mohanty B, Bacal J, et al. Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest. Proc Natl Acad Sci U S A. 2016;113:E3639-48 pubmed publisher
    ..We propose that phosphorylation regulates the recruitment and retention of Tof1-Csm3 by the replisome and that this complex antagonizes the Rrm3 helicase, thereby promoting PFA, by preserving the integrity of the Fob1-Ter complex. ..
  29. Lyons N, Fonslow B, Diedrich J, Yates J, Morgan D. Sequential primed kinases create a damage-responsive phosphodegron on Eco1. Nat Struct Mol Biol. 2013;20:194-201 pubmed publisher
    ..We show here that Eco1 degradation requires the sequential actions of Cdk1 and two additional kinases, Cdc7-Dbf4 and the GSK-3 homolog Mck1...
  30. Yaakov G, Duch A, Garcia Rubio M, Clotet J, Jimenez J, Aguilera A, et al. The stress-activated protein kinase Hog1 mediates S phase delay in response to osmostress. Mol Biol Cell. 2009;20:3572-82 pubmed publisher
    ..The two mechanisms of Hog1 action lead to delayed firing of origins and prolonged replication, respectively. The Hog1-dependent delay of replication could be important to allow Hog1 to induce gene expression before replication. ..
  31. Zarzov P, Boucherie H, Mann C. A yeast heat shock transcription factor (Hsf1) mutant is defective in both Hsc82/Hsp82 synthesis and spindle pole body duplication. J Cell Sci. 1997;110 ( Pt 16):1879-91 pubmed
    ..These results show that Hsf1 is required for spindle pole body duplication at 37 degrees C. ..
  32. Murakami H, Keeney S. Temporospatial coordination of meiotic DNA replication and recombination via DDK recruitment to replisomes. Cell. 2014;158:861-873 pubmed publisher
    ..show that, in yeast, the replisome-associated components Tof1 and Csm3 physically associate with the Dbf4-dependent Cdc7 kinase (DDK) and recruit it to the replisome, where it phosphorylates the DSB-promoting factor Mer2 in the wake of ..
  33. Jeffery D, Kakusho N, You Z, Gharib M, Wyse B, Drury E, et al. CDC28 phosphorylates Cac1p and regulates the association of chromatin assembly factor I with chromatin. Cell Cycle. 2015;14:74-85 pubmed publisher
    ..its Cac1p subunit and the replication sliding clamp, PCNA, and that this interaction is stimulated by the kinase CDC7. Here we show that another kinase, CDC28, phosphorylates Cac1p on serines 94 and 515 in early S phase and regulates ..
  34. Pérez Arnaiz P, Kaplan D. An Mcm10 Mutant Defective in ssDNA Binding Shows Defects in DNA Replication Initiation. J Mol Biol. 2016;428:4608-4625 pubmed publisher
    ..These data suggest that DNA binding by Mcm10 is essential for the initiation of DNA replication. ..
  35. Stead B, Brandl C, Davey M. Phosphorylation of Mcm2 modulates Mcm2-7 activity and affects the cell's response to DNA damage. Nucleic Acids Res. 2011;39:6998-7008 pubmed publisher
    ..We propose that phosphorylation of Mcm2 fine-tunes the activity of Mcm2-7, which in turn modulates DNA replication in response to DNA damage. ..
  36. Buck V, White A, Rosamond J. CDC7 protein kinase activity is required for mitosis and meiosis in Saccharomyces cerevisiae. Mol Gen Genet. 1991;227:452-7 pubmed
    The product of the CDC7 gene of Saccharomyces cerevisiae has multiple cellular functions, being needed for the initiation of DNA synthesis during mitosis as well as for synaptonemal complex formation and commitment to recombination during ..
  37. Nougarede R, Della Seta F, Zarzov P, Schwob E. Hierarchy of S-phase-promoting factors: yeast Dbf4-Cdc7 kinase requires prior S-phase cyclin-dependent kinase activation. Mol Cell Biol. 2000;20:3795-806 pubmed
    ..activation by two S-T protein kinases, an S-phase cyclin-dependent kinase (S-CDK) and a homologue of yeast Dbf4-Cdc7 kinase (Dbf4p-dependent kinase [DDK])...
  38. Yoon H, Campbell J. The CDC7 protein of Saccharomyces cerevisiae is a phosphoprotein that contains protein kinase activity. Proc Natl Acad Sci U S A. 1991;88:3574-8 pubmed
    The CDC7 protein of Saccharomyces cerevisiae may be involved in the G1/S-phase transition and/or in the initiation of mitotic DNA synthesis...
  39. Evrin C, Fernández Cid A, Riera A, Zech J, Clarke P, Herrera M, et al. The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation. Nucleic Acids Res. 2014;42:2257-69 pubmed publisher
    ..Our work identifies the OCM complex as competent for MCM2-7 dimerization, reveals MCM2-7 dimerization as a limiting step during pre-RC formation and defines critical mechanisms that explain how origins are licensed. ..
  40. Dowell S, Romanowski P, Diffley J. Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo. Science. 1994;265:1243-6 pubmed
    ..The Dbf4 protein interacts with and positively regulates the activity of the Cdc7 protein kinase, which is required for entry into S phase in the yeast mitotic cell cycle...
  41. Irlbacher H, Franke J, Manke T, Vingron M, Ehrenhofer Murray A. Control of replication initiation and heterochromatin formation in Saccharomyces cerevisiae by a regulator of meiotic gene expression. Genes Dev. 2005;19:1811-22 pubmed
    ..Full initiation activity of these origins required Sum1, and their origin activity was decreased upon removal of the Sum1-binding site. Thus, Sum1 constitutes a novel global regulator of replication initiation in yeast. ..
  42. Merchan S, Pedelini L, Hueso G, Calzada A, Serrano R, Yenush L. Genetic alterations leading to increases in internal potassium concentrations are detrimental for DNA integrity in Saccharomyces cerevisiae. Genes Cells. 2011;16:152-65 pubmed publisher
    ..Moreover, we observe that this slow growth phenotype observed in cdc7(ts) mutants with reduced Ppz activity is reverted by disrupting the TRK1 potassium transporter gene...
  43. BRANDAO L, Ferguson R, Santoro I, Jinks Robertson S, Sclafani R. The role of Dbf4-dependent protein kinase in DNA polymerase ?-dependent mutagenesis in Saccharomyces cerevisiae. Genetics. 2014;197:1111-22 pubmed publisher
    The yeast Dbf4-dependent kinase (DDK) (composed of Dbf4 and Cdc7 subunits) is an essential, conserved Ser/Thr protein kinase that regulates multiple processes in the cell, including DNA replication, recombination and induced mutagenesis...
  44. Natsume T, Müller C, Katou Y, Retkute R, Gierlinski M, Araki H, et al. Kinetochores coordinate pericentromeric cohesion and early DNA replication by Cdc7-Dbf4 kinase recruitment. Mol Cell. 2013;50:661-74 pubmed publisher
    ..Thus, we have found the central mechanism by which kinetochores orchestrate early S phase DNA replication and robust sister chromatid cohesion at microtubule attachment sites...
  45. Hollingsworth R, Sclafani R. DNA metabolism gene CDC7 from yeast encodes a serine (threonine) protein kinase. Proc Natl Acad Sci U S A. 1990;87:6272-6 pubmed
    The yeast Cdc7 protein is indispensable to initiation of nuclear DNA replication, based on the phenotype of the conditional, temperature-sensitive (ts) cdc7 mutants at the restrictive temperature...
  46. Hardy C. Characterization of an essential Orc2p-associated factor that plays a role in DNA replication. Mol Cell Biol. 1996;16:1832-41 pubmed
    ..These results suggest a role for OAFI in the initiation of DNA replication. Mutant alleles of cdc7 and cdc14 were also isolated in the orc2-1 synthetic lethal screen...
  47. Manfrini N, Gobbini E, Baldo V, Trovesi C, Lucchini G, Longhese M. G(1)/S and G(2)/M cyclin-dependent kinase activities commit cells to death in the absence of the S-phase checkpoint. Mol Cell Biol. 2012;32:4971-85 pubmed publisher
    ..Moreover, these findings suggest that the essential function of Mec1 and Rad53 is not necessarily separated from the function of these kinases in supporting DNA synthesis under stress conditions. ..
  48. Duch A, Felipe Abrio I, Barroso S, Yaakov G, Garcia Rubio M, Aguilera A, et al. Coordinated control of replication and transcription by a SAPK protects genomic integrity. Nature. 2013;493:116-9 pubmed publisher
  49. Ramer M, Suman E, Richter H, Stanger K, Spranger M, Bieberstein N, et al. Dbf4 and Cdc7 proteins promote DNA replication through interactions with distinct Mcm2-7 protein subunits. J Biol Chem. 2013;288:14926-35 pubmed publisher
    The essential cell cycle target of the Dbf4/Cdc7 kinase (DDK) is the Mcm2-7 helicase complex...
  50. Princz L, Wild P, Bittmann J, Aguado F, Blanco M, Matos J, et al. Dbf4-dependent kinase and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis. EMBO J. 2017;36:664-678 pubmed publisher
    ..Here, we show that a third cell cycle kinase, Cdc7-Dbf4 (DDK), targets Mus81-Mms4 in conjunction with Cdc5-both kinases bind to as well as phosphorylate Mus81-Mms4 in ..
  51. Mattarocci S, Shyian M, Lemmens L, Damay P, Altintas D, Shi T, et al. Rif1 controls DNA replication timing in yeast through the PP1 phosphatase Glc7. Cell Rep. 2014;7:62-9 pubmed publisher
    ..Our data thus link Rif1 to negative regulation of replication origin firing through recruitment of the Glc7 phosphatase. ..
  52. Ohtoshi A, Arai K, Masai H. Genetic interactions between CDC7 and CDC28: growth inhibition of cdc28-1N by Cdc7 point mutants. Genes Cells. 1996;1:895-904 pubmed
    b>Cdc7 kinase of Saccharomyces cerevisiae, a nuclear phosphoprotein, regulates initiation of chromosomal DNA replication...
  53. Holzen T, Sclafani R. Genetic interaction of RAD53 protein kinase with histones is important for DNA replication. Cell Cycle. 2010;9:4735-47 pubmed
    ..Deletion of the major histone H3-H4 pair suppresses rad53-rep-cdc7-1 synthetic lethality, suggesting Rad53's functions in degradation of excess cellular histone and in replication ..
  54. Aucher W, Becker E, Ma E, Miron S, Martel A, Ochsenbein F, et al. A strategy for interaction site prediction between phospho-binding modules and their partners identified from proteomic data. Mol Cell Proteomics. 2010;9:2745-59 pubmed publisher
    ..In this screen we detected 11 partners, including Cdc7 and Cdc45, essential components of the DNA replication machinery...
  55. Chen Y, Kenworthy J, Gabrielse C, Hänni C, Zegerman P, Weinreich M. DNA replication checkpoint signaling depends on a Rad53-Dbf4 N-terminal interaction in Saccharomyces cerevisiae. Genetics. 2013;194:389-401 pubmed publisher
    ..This indicated that Rad53 must stably bind to Dbf4 to regulate its activity. ..
  56. Mort Bontemps Soret M, Facca C, Faye G. Physical interaction of Cdc28 with Cdc37 in Saccharomyces cerevisiae. Mol Genet Genomics. 2002;267:447-58 pubmed
    ..In a genetic screen, four protein kinases were identified as interacting with Cdc37 - Cdc5, Cdc7, Cdc15 and Cak1. This result underlines the importance of Cdc37 for the folding of protein kinases...
  57. Sun J, Fernández Cid A, Riera A, Tognetti S, Yuan Z, Stillman B, et al. Structural and mechanistic insights into Mcm2-7 double-hexamer assembly and function. Genes Dev. 2014;28:2291-303 pubmed publisher
    ..The data suggest how the double hexamer is assembled and how helicase activity is regulated during DNA licensing, with implications for cell cycle control of DNA replication and genome stability. ..
  58. Chen X, Suhandynata R, Sandhu R, Rockmill B, Mohibullah N, Niu H, et al. Phosphorylation of the Synaptonemal Complex Protein Zip1 Regulates the Crossover/Noncrossover Decision during Yeast Meiosis. PLoS Biol. 2015;13:e1002329 pubmed publisher
    ..These results suggest that phosphorylation of Zip1 at DSBs commits those breaks to repair via the ZMM pathway and provides a mechanism by which the crossover/noncrossover decision can be dynamically regulated during yeast meiosis. ..
  59. Zhong Y, Nellimoottil T, Peace J, Knott S, Villwock S, Yee J, et al. The level of origin firing inversely affects the rate of replication fork progression. J Cell Biol. 2013;201:373-83 pubmed publisher
    DNA damage slows DNA synthesis at replication forks; however, the mechanisms remain unclear. Cdc7 kinase is required for replication origin activation, is a target of the intra-S checkpoint, and is implicated in the response to ..
  60. Shellman Y, Schauer I, Oshiro G, Dohrmann P, Sclafani R. Oligomers of the Cdc7/Dbf4 protein kinase exist in the yeast cell. Mol Gen Genet. 1998;259:429-36 pubmed
    b>Cdc7/Dbf4 protein kinase is required for the initiation of DNA replication in Saccharomyces cerevisiae...
  61. Dav A, Cooley C, Garg M, Bianchi A. Protein phosphatase 1 recruitment by Rif1 regulates DNA replication origin firing by counteracting DDK activity. Cell Rep. 2014;7:53-61 pubmed publisher
    ..These findings elucidate the mechanism of action of Rif1 in the control of DNA replication and demonstrate a role of PP1 phosphatases in the regulation of origin firing...
  62. Sclafani R, Tecklenburg M, Pierce A. The mcm5-bob1 bypass of Cdc7p/Dbf4p in DNA replication depends on both Cdk1-independent and Cdk1-dependent steps in Saccharomyces cerevisiae. Genetics. 2002;161:47-57 pubmed
    ..Thus Cdc7p and Cdk1p kinases catalyze the initiation of DNA replication at several distinct steps, of which only a subset is bypassed by the mcm5-bob1 mutation. ..
  63. Sasanuma H, Hirota K, Fukuda T, Kakusho N, Kugou K, Kawasaki Y, et al. Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination. Genes Dev. 2008;22:398-410 pubmed publisher
    ..We investigated the meiotic roles of Cdc7 kinase in the initiation of meiotic recombination, namely, DNA double-strand breaks (DSBs) mediated by Spo11 and ..
  64. Herrera M, Tognetti S, Riera A, Zech J, Clarke P, Fernández Cid A, et al. A reconstituted system reveals how activating and inhibitory interactions control DDK dependent assembly of the eukaryotic replicative helicase. Nucleic Acids Res. 2015;43:10238-50 pubmed publisher
    ..Our results indicate that helicase activation is regulated by competitive and cooperative interactions, which restrict illegitimate complex formation and direct limiting helicase-activation factors into pre-initiation complexes. ..
  65. Lydeard J, Lipkin Moore Z, Sheu Y, Stillman B, Burgers P, Haber J. Break-induced replication requires all essential DNA replication factors except those specific for pre-RC assembly. Genes Dev. 2010;24:1133-44 pubmed publisher
    ..The Cdc7 kinase, required for both initiation of DNA replication and post-replication repair (PRR), is also required for BIR...
  66. Steere N, Yamaguchi S, Andrews C, Liachko I, Nakamura T, Shima N. Functional screen of human MCM2-7 variant alleles for disease-causing potential. Mutat Res. 2009;666:74-8 pubmed publisher
    ..This screen identified a MCM5 variant allele with pathogenic potential. This allele deserves further investigations on its effect on cancer development in human populations. ..