Gene Symbol: CDC6
Description: AAA family ATPase CDC6
Alias: AAA family ATPase CDC6
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Bowers J, Randell J, Chen S, Bell S. ATP hydrolysis by ORC catalyzes reiterative Mcm2-7 assembly at a defined origin of replication. Mol Cell. 2004;16:967-78 pubmed
  2. Chen Z, Speck C, Wendel P, Tang C, Stillman B, Li H. The architecture of the DNA replication origin recognition complex in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2008;105:10326-31 pubmed publisher
    ..complex bind to origins of DNA replication and recruit prereplicative complex (pre-RC) proteins, one of which is Cdc6. To further understand the function of ORC we recently determined by single-particle reconstruction of electron ..
  3. Seki T, Diffley J. Stepwise assembly of initiation proteins at budding yeast replication origins in vitro. Proc Natl Acad Sci U S A. 2000;97:14115-20 pubmed
    ..Extracts from cells arrested in mitosis also can support the binding of ORC but are unable to load either Cdc6p or MCM2-7 proteins. This system should be useful for studying the mechanism and regulation of pre-RC assembly. ..
  4. Evrin C, Fernández Cid A, Zech J, Herrera M, Riera A, Clarke P, et al. In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization. Nucleic Acids Res. 2013;41:3162-72 pubmed publisher
    The origin recognition complex (ORC) of Saccharomyces cerevisiae binds origin DNA and cooperates with Cdc6 and Cdt1 to load the replicative helicase MCM2-7 onto DNA...
  5. Schepers A, Diffley J. Mutational analysis of conserved sequence motifs in the budding yeast Cdc6 protein. J Mol Biol. 2001;308:597-608 pubmed
    The Cdc6 protein is required to load a complex of Mcm2-7 family members (the MCM complex) into prereplicative complexes at budding yeast origins of DNA replication...
  6. Green B, Li J. Loss of rereplication control in Saccharomyces cerevisiae results in extensive DNA damage. Mol Biol Cell. 2005;16:421-32 pubmed
    ..Hence, even subtle disruptions in the cell cycle regulation of DNA replication may predispose cells to the genomic instability associated with tumorigenesis. ..
  7. Liang C, Weinreich M, Stillman B. ORC and Cdc6p interact and determine the frequency of initiation of DNA replication in the genome. Cell. 1995;81:667-76 pubmed
    ..At the restrictive temperature, the number of active replicators was diminished further. Using a genetic screen, CDC6 was identified as a multicopy suppressor of orc5-1...
  8. Drury L, Perkins G, Diffley J. The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast. EMBO J. 1997;16:5966-76 pubmed
    The budding yeast Cdc6 protein (Cdc6p) is essential for formation of pre-replicative complexes (pre-RCs) at origins of DNA replication...
  9. Mimura S, Seki T, Tanaka S, Diffley J. Phosphorylation-dependent binding of mitotic cyclins to Cdc6 contributes to DNA replication control. Nature. 2004;431:1118-23 pubmed
    ..CDKs inhibit each pre-RC component (ORC, Cdc6, Cdt1/Mcm2-7) by different mechanisms...

More Information


  1. Zwerschke W, Rottjakob H, Küntzel H. The Saccharomyces cerevisiae CDC6 gene is transcribed at late mitosis and encodes a ATP/GTPase controlling S phase initiation. J Biol Chem. 1994;269:23351-6 pubmed
    We have compared the periodic fluctuation of mRNAs encoded by CDC6, a cell cycle gene controlling initiation of DNA replication, and CLN1, a G1 cyclin gene expressed at late G1...
  2. Speck C, Stillman B. Cdc6 ATPase activity regulates ORC x Cdc6 stability and the selection of specific DNA sequences as origins of DNA replication. J Biol Chem. 2007;282:11705-14 pubmed
    ..To initiate DNA replication ORC requires Cdc6 to target initiation to specific DNA sequences in chromosomes and with Cdt1 loads the ring-shaped mini-chromosome ..
  3. Sanchez M, Calzada A, Bueno A. The Cdc6 protein is ubiquitinated in vivo for proteolysis in Saccharomyces cerevisiae. J Biol Chem. 1999;274:9092-7 pubmed
    The Saccharomyces cerevisiae Cdc6 protein is necessary for the formation of pre-replicative complexes that are required for firing DNA replication at origins at the beginning of S phase...
  4. Randell J, Bowers J, Rodríguez H, Bell S. Sequential ATP hydrolysis by Cdc6 and ORC directs loading of the Mcm2-7 helicase. Mol Cell. 2006;21:29-39 pubmed called the prereplicative complex (pre-RC), and its formation requires the origin recognition complex (ORC), Cdc6, Cdt1, and ATP. ATP hydrolysis by ORC is required for multiple rounds of Mcm2-7 loading...
  5. Fernández Cid A, Riera A, Tognetti S, Herrera M, Samel S, Evrin C, et al. An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly. Mol Cell. 2013;50:577-88 pubmed publisher
    ..higher eukaryotes, the loading of the replicative helicase MCM2-7 onto DNA requires the combined activities of ORC, Cdc6, and Cdt1. These proteins load MCM2-7 in an unknown way into a double hexamer around DNA...
  6. Sun J, Evrin C, Samel S, Fernández Cid A, Riera A, Kawakami H, et al. Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA. Nat Struct Mol Biol. 2013;20:944-51 pubmed publisher
    In eukaryotes, the Cdt1-bound replicative helicase core MCM2-7 is loaded onto DNA by the ORC-Cdc6 ATPase to form a prereplicative complex (pre-RC) with an MCM2-7 double hexamer encircling DNA...
  7. Cocker J, Piatti S, Santocanale C, Nasmyth K, Diffley J. An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast. Nature. 1996;379:180-2 pubmed
    ..We show here that the Cdc6 protein (Cdc6p), which is necessary for origin firing in vivo, is essential for the establishment and maintenance ..
  8. Perkins G, Drury L, Diffley J. Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis. EMBO J. 2001;20:4836-45 pubmed
    The Cdc6 DNA replication initiation factor is targeted for ubiquitin-mediated proteolysis by the E3 ubiquitin ligase SCF(CDC4) from the end of G1phase until mitosis in the budding yeast Saccharomyces cerevisiae...
  9. Honey S, Futcher B. Roles of the CDK phosphorylation sites of yeast Cdc6 in chromatin binding and rereplication. Mol Biol Cell. 2007;18:1324-36 pubmed
    The Saccharomyces cerevisiae Cdc6 protein is crucial for DNA replication. In the absence of cyclin-dependent kinase (CDK) activity, Cdc6 binds to replication origins, and loads Mcm proteins...
  10. Donovan S, Harwood J, Drury L, Diffley J. Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast. Proc Natl Acad Sci U S A. 1997;94:5611-6 pubmed
    The Cdc6 protein is essential for the assembly of pre-replicative complexes (pre-RCs) at origins of DNA replication in the budding yeast Saccharomyces cerevisiae...
  11. Elsasser S, Chi Y, Yang P, Campbell J. Phosphorylation controls timing of Cdc6p destruction: A biochemical analysis. Mol Biol Cell. 1999;10:3263-77 pubmed
    ..The stable cdc6 mutants show no apparent replication defects in wild-type strains...
  12. Ikui A, Archambault V, Drapkin B, Campbell V, Cross F. Cyclin and cyclin-dependent kinase substrate requirements for preventing rereplication reveal the need for concomitant activation and inhibition. Genetics. 2007;175:1011-22 pubmed enforced by cyclin-Cdk-dependent phosphorylation of the prereplicative complex (pre-RC) components Mcm2-7, Cdc6, and Orc1-6. Several of these controls must be simultaneously blocked by mutation to obtain rereplication...
  13. Wilmes G, Archambault V, Austin R, Jacobson M, Bell S, Cross F. Interaction of the S-phase cyclin Clb5 with an "RXL" docking sequence in the initiator protein Orc6 provides an origin-localized replication control switch. Genes Dev. 2004;18:981-91 pubmed
    ..We propose that Clb5 binding to ORC provides an origin-localized replication control switch that specifically prevents reinitiation at replicated origins. ..
  14. Archambault V, Li C, Tackett A, Wasch R, Chait B, Rout M, et al. Genetic and biochemical evaluation of the importance of Cdc6 in regulating mitotic exit. Mol Biol Cell. 2003;14:4592-604 pubmed
    We evaluated the hypothesis that the N-terminal region of the replication control protein Cdc6 acts as an inhibitor of cyclin-dependent kinase (Cdk) activity, promoting mitotic exit...
  15. Kawasaki Y, Kim H, Kojima A, Seki T, Sugino A. Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells. 2006;11:745-56 pubmed
  16. Loog M, Morgan D. Cyclin specificity in the phosphorylation of cyclin-dependent kinase substrates. Nature. 2005;434:104-8 pubmed
    ..The Clb5-specific targets include several proteins (Sld2, Cdc6, Orc6, Mcm3 and Cdh1) involved in early S-phase events...
  17. Ikui A, Rossio V, Schroeder L, Yoshida S. A yeast GSK-3 kinase Mck1 promotes Cdc6 degradation to inhibit DNA re-replication. PLoS Genet. 2012;8:e1003099 pubmed publisher
    ..Here we show that Mck1p, a yeast homologue of GSK-3 kinase, is also required for Cdc6 degradation through a distinct mechanism...
  18. Speck C, Chen Z, Li H, Stillman B. ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA. Nat Struct Mol Biol. 2005;12:965-71 pubmed
    Binding of Cdc6 to the origin recognition complex (ORC) is a key step in the assembly of a pre-replication complex (pre-RC) at origins of DNA replication. ORC recognizes specific origin DNA sequences in an ATP-dependent manner...
  19. Boronat S, Campbell J. Mitotic Cdc6 stabilizes anaphase-promoting complex substrates by a partially Cdc28-independent mechanism, and this stabilization is suppressed by deletion of Cdc55. Mol Cell Biol. 2007;27:1158-71 pubmed
    ..Abolishing Cdc6p/Cdc28p interaction does not eliminate the Cdc6-dependent delay of these anaphase events...
  20. Al Zain A, Schroeder L, Sheglov A, Ikui A. Cdc6 degradation requires phosphodegron created by GSK-3 and Cdk1 for SCFCdc4 recognition in Saccharomyces cerevisiae. Mol Biol Cell. 2015;26:2609-19 pubmed publisher
    ..Cdc6p is part of the prereplicative complex, which is essential for DNA replication. Cdc6 is phosphorylated by cyclin-Cdk1 to promote its degradation after origin firing to prevent DNA rereplication...
  21. Li J, Tetzlaff M, Elledge S. Identification of MSA1, a cell cycle-regulated, dosage suppressor of drc1/sld2 and dpb11 mutants. Cell Cycle. 2008;7:3388-98 pubmed
    ..Paradoxically, MSA1 overproduction is deleterious to cdc6-1, cdc7-1, cdc28-1N and cdc14-1 mutants indicating a complex relationship with DNA replication and cell cycle ..
  22. Drury L, Diffley J. Factors affecting the diversity of DNA replication licensing control in eukaryotes. Curr Biol. 2009;19:530-5 pubmed publisher
    ..APC/C) restrict licensing to G1 phase by directly and indirectly regulating pre-RC components, including ORC, Cdc6, Cdt1, and Mcm2-7...
  23. Lydall D, Weinert T. G2/M checkpoint genes of Saccharomyces cerevisiae: further evidence for roles in DNA replication and/or repair. Mol Gen Genet. 1997;256:638-51 pubmed
    ..proteins Rfc1p (cdc44), DNA pol alpha (cdc17) and DNA pol delta (cdc2) but has much weaker effects on cdc6, cdc9, cdc15 and CDC4 strains...
  24. Kawakami H, Ohashi E, Kanamoto S, Tsurimoto T, Katayama T. Specific binding of eukaryotic ORC to DNA replication origins depends on highly conserved basic residues. Sci Rep. 2015;5:14929 pubmed publisher
    ..Lys-362 and Arg-367 residues of Orc1 are highly conserved among eukaryotic ORCs, but not in eubacterial and archaeal orthologs, suggesting a eukaryote-specific mechanism underlying recognition of replication origins by ORC. ..
  25. Irlbacher H, Franke J, Manke T, Vingron M, Ehrenhofer Murray A. Control of replication initiation and heterochromatin formation in Saccharomyces cerevisiae by a regulator of meiotic gene expression. Genes Dev. 2005;19:1811-22 pubmed
    ..Full initiation activity of these origins required Sum1, and their origin activity was decreased upon removal of the Sum1-binding site. Thus, Sum1 constitutes a novel global regulator of replication initiation in yeast. ..
  26. Comino A, Mancek M, Komel R. Ecm11 is located in the cell nucleus throughout mitosis. Pflugers Arch. 2001;442:R179-81 pubmed
    It was found previously that Ecm11 interacts with Cdc6 in a two-hybrid screen. To verify that these two gene products can actually encounter in the living cell, ECM11 was expressed as a fusion with GFP...
  27. Formosa T, Nittis T. Suppressors of the temperature sensitivity of DNA polymerase alpha mutations in Saccharomyces cerevisiae. Mol Gen Genet. 1998;257:461-8 pubmed
    ..partially suppressed a mutation in POL3 which encodes DNA polymerase delta, and both also affected a mutation in CDC6, which acts in initiation of DNA replication...
  28. Kono K, Al Zain A, Schroeder L, Nakanishi M, Ikui A. Plasma membrane/cell wall perturbation activates a novel cell cycle checkpoint during G1 in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2016;113:6910-5 pubmed publisher
    ..that the inhibition of DNA replication upon plasma membrane damage requires GSK3/Mck1-dependent degradation of Cdc6, a component of the prereplicative complex...
  29. Zavec P, Comino A, Watt P, Komel R. Interaction trap experiment with CDC6. Pflugers Arch. 2000;439:r094-r096 pubmed publisher
    b>CDC6 is an essential gene of yeast Saccharomyces cerevisiae. Although DNA sequence of the gene is available for a long time, biochemical function of Cdc6 protein in the cell cycle remains unclear...
  30. Feng L, Wang B, Jong A. Saccharomyces cerevisiae Cdc6 stimulates Abf1 DNA binding activity. J Biol Chem. 1998;273:1298-302 pubmed
    ..We describe in this study how yeast Cdc6 protein stimulates Abf1 protein DNA binding activities...
  31. Cross F, Schroeder L, Kruse M, Chen K. Quantitative characterization of a mitotic cyclin threshold regulating exit from mitosis. Mol Biol Cell. 2005;16:2129-38 pubmed
    ..The loss of robustness of the Clb2 overexpressing system is also predicted by the model. These results provide strong confirmation of the model's predictive ability. ..
  32. Lottersberger F, Rubert F, Baldo V, Lucchini G, Longhese M. Functions of Saccharomyces cerevisiae 14-3-3 proteins in response to DNA damage and to DNA replication stress. Genetics. 2003;165:1717-32 pubmed
    ..Finally, the bmh1-169 bmh2Delta and bmh1-170 bmh2Delta mutants show increased rates of spontaneous gross chromosomal rearrangements, indicating that Bmh proteins are required to suppress genome instability. ..
  33. Loo S, Fox C, Rine J, Kobayashi R, Stillman B, Bell S. The origin recognition complex in silencing, cell cycle progression, and DNA replication. Mol Biol Cell. 1995;6:741-56 pubmed
    ..The silencing defect caused by orc5-1 strengthened previous connections between ORC and silencing, and combined with the phenotypes caused by orc2 mutations, suggested that the complex itself functions in both processes. ..
  34. Zhai Y, Yung P, Huo L, Liang C. Cdc14p resets the competency of replication licensing by dephosphorylating multiple initiation proteins during mitotic exit in budding yeast. J Cell Sci. 2010;123:3933-43 pubmed publisher
  35. Calzada A, Sacristan M, Sanchez E, Bueno A. Cdc6 cooperates with Sic1 and Hct1 to inactivate mitotic cyclin-dependent kinases. Nature. 2001;412:355-8 pubmed
    ..Several striking similarities exist between Sic1 and Cdc6, a DNA replication factor essential for the formation of pre-replicative complexes at origins of DNA replication...
  36. Lorenz M, Heitman J. Regulators of pseudohyphal differentiation in Saccharomyces cerevisiae identified through multicopy suppressor analysis in ammonium permease mutant strains. Genetics. 1998;150:1443-57 pubmed
    ..Multicopy expression of TEC1, PHD1, PHD2 (MSS10/MSN1/FUP4), MSN5, CDC6, MSS11, MGA1, SKN7, DOT6, HMS1, HMS2, or MEP2 each restored filamentation in a Deltamep1/Deltamep1 Deltamep2/..
  37. Pappas D, Frisch R, Weinreich M. The NAD(+)-dependent Sir2p histone deacetylase is a negative regulator of chromosomal DNA replication. Genes Dev. 2004;18:769-81 pubmed
    ..the genetic requirements for pre-RC formation, we selected chromosomal suppressors of a temperature-sensitive cdc6-4 mutant defective for pre-RC assembly...
  38. Klemm R, Bell S. ATP bound to the origin recognition complex is important for preRC formation. Proc Natl Acad Sci U S A. 2001;98:8361-7 pubmed
    ..Based on these observations, we propose that Cdc6p specifically recognizes the ATP-bound state of Orc1p and that ATP hydrolysis is coupled to preRC disassembly. ..
  39. Crampton A, Chang F, Pappas D, Frisch R, Weinreich M. An ARS element inhibits DNA replication through a SIR2-dependent mechanism. Mol Cell. 2008;30:156-66 pubmed publisher
    ..These data suggest that Sir2p and I(S) elements inhibit origin activity by promoting an unfavorable chromatin structure for pre-RC assembly...
  40. Zavec P, Comino A, Watt P, Komel R. Interaction trap experiment with CDC6. Pflugers Arch. 2000;439:R94-6 pubmed
    b>CDC6 is an essential gene of yeast Saccharomyces cerevisiae. Although DNA sequence of the gene is available for a long time, biochemical function of Cdc6 protein in the cell cycle remains unclear...
  41. Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, et al. Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. Nat Struct Mol Biol. 2017;24:316-324 pubmed publisher
    To initiate DNA replication, the origin recognition complex (ORC) and Cdc6 load an Mcm2-7 double hexamer onto DNA...
  42. Zou L, Stillman B. Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science. 1998;280:593-6 pubmed
  43. Rancati G, Crispo V, Lucchini G, Piatti S. Mad3/BubR1 phosphorylation during spindle checkpoint activation depends on both Polo and Aurora kinases in budding yeast. Cell Cycle. 2005;4:972-80 pubmed
    ..Accordingly, replacing with alanines five serine residues belonging to Polo kinase-dependent putative phosphorylation sites dramatically reduces Mad3 phosphorylation, suggesting that Mad3 is likely an in vivo target of Cdc5. ..
  44. Bremmer S, Hall H, Martinez J, Eissler C, Hinrichsen T, Rossie S, et al. Cdc14 phosphatases preferentially dephosphorylate a subset of cyclin-dependent kinase (Cdk) sites containing phosphoserine. J Biol Chem. 2012;287:1662-9 pubmed publisher
    ..The intrinsic selectivity of Cdc14 may help establish the order of Cdk substrate dephosphorylation during mitotic exit and contribute to roles in other cellular processes. ..
  45. Kim D, Zhang W, Koepp D. The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase. J Biol Chem. 2012;287:44212-20 pubmed publisher
    The accurate replication of genetic information is critical to maintaining chromosomal integrity. Cdc6 functions in the assembly of pre-replicative complexes and is specifically required to load the Mcm2-7 replicative helicase complex at ..
  46. Kroll E, Hyland K, Hieter P, Li J. Establishing genetic interactions by a synthetic dosage lethality phenotype. Genetics. 1996;143:95-102 pubmed
    ..ORC6 overexpression caused synthetic dosage lethality in combination with cdc2-1, cdc6-1, cdc14-1, cdc16-1 and cdc46-1...
  47. Hahn S, Maurer P, Caesar S, Schlenstedt G. Classical NLS proteins from Saccharomyces cerevisiae. J Mol Biol. 2008;379:678-94 pubmed publisher
    ..We show for the first time that endogenous S. cerevisiae proteins Prp20, Cdc6, Swi5, Cdc45, and Clb2 fulfill all of these criteria identifying them as authentic yeast cNLS cargos...
  48. Laha S, Das S, Hajra S, Sau S, Sinha P. The budding yeast protein Chl1p is required to preserve genome integrity upon DNA damage in S-phase. Nucleic Acids Res. 2006;34:5880-91 pubmed
    ..Our work suggests that Chl1p is required for genome integrity when cells suffer endogenously or exogenously induced DNA damage. ..
  49. Blitzblau H, Hochwagen A. ATR/Mec1 prevents lethal meiotic recombination initiation on partially replicated chromosomes in budding yeast. elife. 2013;2:e00844 pubmed publisher
    ..DOI: ..
  50. Tanaka S, Araki H. Multiple regulatory mechanisms to inhibit untimely initiation of DNA replication are important for stable genome maintenance. PLoS Genet. 2011;7:e1002136 pubmed publisher
    ..These findings highlight the importance of having multiple inhibitory mechanisms to prevent the untimely initiation of chromosome replication to preserve stable genome maintenance over generations in eukaryotes. ..
  51. Fujita M, Hori Y, Shirahige K, Tsurimoto T, Yoshikawa H, Obuse C. Cell cycle dependent topological changes of chromosomal replication origins in Saccharomyces cerevisiae. Genes Cells. 1998;3:737-49 pubmed
    ..Functional CDC6 and MCM5 were required for maintaining the weaker inhibition state in G1-arrested cells...
  52. Rehman M, Fourel G, Mathews A, Ramdin D, Espinosa M, Gilson E, et al. Differential requirement of DNA replication factors for subtelomeric ARS consensus sequence protosilencers in Saccharomyces cerevisiae. Genetics. 2006;174:1801-10 pubmed
    ..Here we analyze the effect of mutations in DNA replication factors (mcm5-461, mcm5-1, orc2-1, orc5-1, cdc45-1, cdc6-1, and cdc7-1) on the silencing of a group of reporter constructs, which contain different combinations of "..
  53. Makrantoni V, Stark M. Efficient chromosome biorientation and the tension checkpoint in Saccharomyces cerevisiae both require Bir1. Mol Cell Biol. 2009;29:4552-62 pubmed publisher
    ..The role of Bir1 therefore may become more critical when spindle formation is delayed. ..
  54. Aparicio O, Weinstein D, Bell S. Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell. 1997;91:59-69 pubmed
    ..Our results identify protein components of the pre-RC and a novel replication complex appearing at the G1/S transition (the RC), and suggest that after initiation MCM proteins and Cdc45p move with eukaryotic replication forks. ..
  55. Weinreich M, Liang C, Chen H, Stillman B. Binding of cyclin-dependent kinases to ORC and Cdc6p regulates the chromosome replication cycle. Proc Natl Acad Sci U S A. 2001;98:11211-7 pubmed
  56. Feng L, Hu Y, Wang B, Wu L, Jong A. Loss control of Mcm5 interaction with chromatin in cdc6-1 mutated in CDC-NTP motif. DNA Cell Biol. 2000;19:447-57 pubmed
    Saccharomyces cerevisiae Cdc6 plays an essential role in establishing and maintaining the prereplicative complex (pre-RC) by interacting with the origin recognition complex (ORC) and associating with chromatin origins...
  57. Evrin C, Fernández Cid A, Riera A, Zech J, Clarke P, Herrera M, et al. The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation. Nucleic Acids Res. 2014;42:2257-69 pubmed publisher
    ..At first, ORC/Cdc6 recruits with the help of Cdt1 a single MCM2-7 hexamer to form an 'initial' ORC/Cdc6/Cdt1/MCM2-7 complex...
  58. Tanaka T, Knapp D, Nasmyth K. Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs. Cell. 1997;90:649-60 pubmed
    ..This characteristic is thought to be due to the properties of "initiation" proteins like Orc, Cdc6, and Mcms...
  59. McGrath D, Balog E, Kõivomägi M, Lucena R, Mai M, Hirschi A, et al. Cks confers specificity to phosphorylation-dependent CDK signaling pathways. Nat Struct Mol Biol. 2013;20:1407-14 pubmed publisher
    ..Together, our results portray Cks as a multifunctional phosphoadaptor that serves as a specificity factor for CDK activity. ..
  60. Elsasser S, Lou F, Wang B, Campbell J, Jong A. Interaction between yeast Cdc6 protein and B-type cyclin/Cdc28 kinases. Mol Biol Cell. 1996;7:1723-35 pubmed
    During purification of recombinant Cdc6 expressed in yeast, we found that Cdc6 interacts with the critical cell cycle, cyclin-dependent protein kinase Cdc28...
  61. Kõivomägi M, Ord M, Iofik A, Valk E, Venta R, Faustova I, et al. Multisite phosphorylation networks as signal processors for Cdk1. Nat Struct Mol Biol. 2013;20:1415-24 pubmed publisher
  62. Ofir Y, Sagee S, Guttmann Raviv N, Pnueli L, Kassir Y. The role and regulation of the preRC component Cdc6 in the initiation of premeiotic DNA replication. Mol Biol Cell. 2004;15:2230-42 pubmed
    ..In this report, we examine the role of Cdc6, a component of the prereplication complex, in the initiation of premeiotic DNA replication in budding yeast...
  63. Wang B, Feng L, Hu Y, Huang S, Reynolds C, Wu L, et al. The essential role of Saccharomyces cerevisiae CDC6 nucleotide-binding site in cell growth, DNA synthesis, and Orc1 association. J Biol Chem. 1999;274:8291-8 pubmed
    Saccharomyces cerevisiae Cdc6 is a protein required for the initiation of DNA replication. The biochemical function of the protein is unknown, but the primary sequence contains motifs characteristic of nucleotide-binding sites...
  64. Huo L, Wu R, Yu Z, Zhai Y, Yang X, Chan T, et al. The Rix1 (Ipi1p-2p-3p) complex is a critical determinant of DNA replication licensing independent of their roles in ribosome biogenesis. Cell Cycle. 2012;11:1325-39 pubmed publisher
    ..These results establish a new framework for the hierarchy of pre-RC proteins, where the Ipi1p-2p-3p complex provides a critical link between ORC-Noc3p and Cdc6p-Cdt1p-MCM in replication licensing. ..
  65. Gómez González B, Felipe Abrio I, Aguilera A. The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants. Mol Cell Biol. 2009;29:5203-13 pubmed publisher
    ..In light of these results, we propose a model in which R-loop-mediated recombination is explained by template switching. ..
  66. Calzada A, Sanchez M, Sanchez E, Bueno A. The stability of the Cdc6 protein is regulated by cyclin-dependent kinase/cyclin B complexes in Saccharomyces cerevisiae. J Biol Chem. 2000;275:9734-41 pubmed
    The Saccharomyces cerevisiae Cdc6 protein is necessary for the formation of prereplicative complexes that are a prerequisite for firing origins during DNA replication in the S phase...
  67. Jang S, Elsasser S, Campbell J, Kim J. Identification of Cdc6 protein domains involved in interaction with Mcm2 protein and Cdc4 protein in budding yeast cells. Biochem J. 2001;354:655-61 pubmed
    The Cdc6 protein (Cdc6p) has essential roles in regulating initiation of DNA replication...