CDC5

Summary

Gene Symbol: CDC5
Description: polo kinase CDC5
Alias: MSD2, PKX2, polo kinase CDC5
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Maekawa H, Priest C, Lechner J, Pereira G, Schiebel E. The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal. J Cell Biol. 2007;179:423-36 pubmed
    ..This allows Kin4 to phosphorylate the SPB-associated Bfa1, rendering it resistant to inactivation by Cdc5 polo kinase...
  2. Wang Y, Shirogane T, Liu D, Harper J, Elledge S. Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling. Cell. 2003;112:697-709 pubmed
    ..Thus, Amn1 is part of a daughter-specific switch that helps cells exit from mitotic exit and reset the cell cycle. ..
  3. Shirayama M, Matsui Y, Toh e A. The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase. Mol Cell Biol. 1994;14:7476-82 pubmed
    ..The genetic interaction among LTE1, TEM1, and CDC15 indicates that they cooperatively play an essential role for termination of M phase. ..
  4. Acosta I, Ontoso D, San Segundo P. The budding yeast polo-like kinase Cdc5 regulates the Ndt80 branch of the meiotic recombination checkpoint pathway. Mol Biol Cell. 2011;22:3478-90 pubmed publisher
    ..We report that overproduction of the polo-like kinase Cdc5 partially alleviates the meiotic prophase arrest of zip1, leading to the formation of inviable meiotic products...
  5. Vidanes G, Sweeney F, Galicia S, Cheung S, Doyle J, Durocher D, et al. CDC5 inhibits the hyperphosphorylation of the checkpoint kinase Rad53, leading to checkpoint adaptation. PLoS Biol. 2010;8:e1000286 pubmed publisher
    The Saccharomyces cerevisiae polo-like kinase Cdc5 promotes adaptation to the DNA damage checkpoint, in addition to its numerous roles in mitotic progression...
  6. Ro H, Song S, Lee K. Bfa1 can regulate Tem1 function independently of Bub2 in the mitotic exit network of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2002;99:5436-41 pubmed
    ..Here, we have demonstrated that introduction of bfa1 Delta suppresses the growth defects associated with the cdc5-1 mutation significantly better than that of bub2 Delta, suggesting that Bfa1 may have a previously uncharacterized ..
  7. Matos J, Blanco M, Maslen S, Skehel J, West S. Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis. Cell. 2011;147:158-72 pubmed publisher
    ..Mus81-Mms4 is hyperactivated by Cdc5-mediated phosphorylation in meiosis I, generating the crossovers necessary for chromosome segregation...
  8. Yu H, Koshland D. Chromosome morphogenesis: condensin-dependent cohesin removal during meiosis. Cell. 2005;123:397-407 pubmed
    ..Here we use budding yeast Saccharomyces cerevisiae to show that condensin and Cdc5, a Polo-like kinase, facilitate the removal of cohesin from chromosomes prior to the onset of anaphase I when ..
  9. Iacovella M, Daly C, Kelly J, Michielsen A, Clyne R. Analysis of Polo-like kinase Cdc5 in the meiosis recombination checkpoint. Cell Cycle. 2010;9:1182-93 pubmed
    ..The budding yeast Polo-like kinase, Cdc5, has been identified as a key regulator of the meiosis I chromosome segregation pattern...

More Information

Publications63

  1. Donnianni R, Ferrari M, Lazzaro F, Clerici M, Tamilselvan Nachimuthu B, Plevani P, et al. Elevated levels of the polo kinase Cdc5 override the Mec1/ATR checkpoint in budding yeast by acting at different steps of the signaling pathway. PLoS Genet. 2010;6:e1000763 pubmed publisher
    ..Here, we report that, in budding yeast, overproduction of the Cdc5 polo kinase overrides the checkpoint signaling induced by double strand DNA breaks (DSBs), preventing the ..
  2. Galgoczy D, Toczyski D. Checkpoint adaptation precedes spontaneous and damage-induced genomic instability in yeast. Mol Cell Biol. 2001;21:1710-8 pubmed
    ..Because malignant progression frequently involves loss of genes that function in DNA repair, adaptation may promote tumorigenesis by allowing genomic instability to occur in the absence of repair. ..
  3. Rudner A, Murray A. Phosphorylation by Cdc28 activates the Cdc20-dependent activity of the anaphase-promoting complex. J Cell Biol. 2000;149:1377-90 pubmed
    ..Previous reports have shown that the budding yeast Cdc5 homologue, Plk, can also phosphorylate and activate the APC in vitro...
  4. Frenz L, Lee S, Fesquet D, Johnston L. The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis. J Cell Sci. 2000;113 Pt 19:3399-408 pubmed
    ..Localisation to the bud neck depends upon the septins and also the mitotic exit network proteins Mob1, Cdc5, Cdc14 and Cdc15. The above data are consistent with Dbf2 acting downstream in a pathway controlling cytokinesis.
  5. Bartholomew C, Woo S, Chung Y, Jones C, Hardy C. Cdc5 interacts with the Wee1 kinase in budding yeast. Mol Cell Biol. 2001;21:4949-59 pubmed
    ..Overexpression of wild-type Polo-like kinase Cdc5 or a catalytically inactive form resulted in the formation of multinucleate cells in budding yeast...
  6. Kim J, Jeong J, Song K. The C-terminus of Bfa1p in budding yeast is essential to induce mitotic arrest in response to diverse checkpoint-activating signals. Genes Cells. 2004;9:399-418 pubmed
  7. Geymonat M, Spanos A, Walker P, Johnston L, Sedgwick S. In vitro regulation of budding yeast Bfa1/Bub2 GAP activity by Cdc5. J Biol Chem. 2003;278:14591-4 pubmed
    The Cdc5 protein of budding yeast is a polo-like kinase that has multiple roles in mitosis including control of the mitotic exit network (MEN)...
  8. Yoshida S, Kono K, Lowery D, Bartolini S, Yaffe M, Ohya Y, et al. Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis. Science. 2006;313:108-11 pubmed
    ..We found that budding yeast Polo-like kinase Cdc5 controls the targeting and activation of Rho1 (RhoA) at the division site via Rho1 guanine nucleotide exchange ..
  9. Visintin R, Stegmeier F, Amon A. The role of the polo kinase Cdc5 in controlling Cdc14 localization. Mol Biol Cell. 2003;14:4486-98 pubmed
    ..Furthermore, we examine the role of the protein kinase Cdc5, which is a component of both the FEAR network and the MEN, in Cdc14 release from the nucleolus...
  10. Simpson Lavy K, Brandeis M. Clb2 and the APC/C(Cdh1) regulate Swe1 stability. Cell Cycle. 2010;9:3046-53 pubmed publisher
    ..The APC/C(Cdh1) substrates Cdc5, Clb2 and Hsl1 regulate Swe1 degradation. We observed that clb2Deltacdh1Delta double mutant S...
  11. Gruneberg U, Campbell K, Simpson C, Grindlay J, Schiebel E. Nud1p links astral microtubule organization and the control of exit from mitosis. EMBO J. 2000;19:6475-88 pubmed
    ..Thus, in nud1-2 cells the failure of Tem1p to interact with Cdc15p at the SPB probably prevents mitotic exit. ..
  12. Rudner A, Hardwick K, Murray A. Cdc28 activates exit from mitosis in budding yeast. J Cell Biol. 2000;149:1361-76 pubmed
    ..The defects of CDC28-VF suggest that Cdc28 activity is required to induce the metaphase to anaphase transition and initiate the transition from anaphase to G1 in budding yeast...
  13. Leroy C, Lee S, Vaze M, Ochsenbein F, Ochsenbien F, Guerois R, et al. PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand break. Mol Cell. 2003;11:827-35 pubmed
    ..In contrast, overexpression of PTC2 rescues adaptation-defective yku80Delta and cdc5-ad mutants. These effects are not explained by alterations either in the processing of DSB ends or in DSB repair...
  14. Snead J, Sullivan M, Lowery D, Cohen M, Zhang C, Randle D, et al. A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration. Chem Biol. 2007;14:1261-72 pubmed
    ..Specific pharmacological inhibition of budding yeast Polo-like kinase, Cdc5, resulted in a misaligned preanaphase spindle and subsequently delayed anaphase nuclear migration, revealing a Cdc5 ..
  15. Song S, Lee K. A novel function of Saccharomyces cerevisiae CDC5 in cytokinesis. J Cell Biol. 2001;152:451-69 pubmed
    ..The Saccharomyces cerevisiae polo kinase Cdc5 functions in a pathway leading to the degradation of mitotic cyclin Clb2, thereby permitting mitotic exit...
  16. Shou W, Deshaies R. Multiple telophase arrest bypassed (tab) mutants alleviate the essential requirement for Cdc15 in exit from mitosis in S. cerevisiae. BMC Genet. 2002;3:4 pubmed
    ..or cdc14Delta, arguing that whereas the essential task of the first four genes is to promote exit from mitosis, CDC5 possesses additional essential function(s)...
  17. Song S, Grenfell T, Garfield S, Erikson R, Lee K. Essential function of the polo box of Cdc5 in subcellular localization and induction of cytokinetic structures. Mol Cell Biol. 2000;20:286-98 pubmed
    ..Here we show that the budding-yeast polo kinase Cdc5, when fused to green fluorescent protein and expressed under its endogenous promoter, localizes at spindle ..
  18. Asano S, Park J, Sakchaisri K, Yu L, Song S, Supavilai P, et al. Concerted mechanism of Swe1/Wee1 regulation by multiple kinases in budding yeast. EMBO J. 2005;24:2194-204 pubmed
    ..Here, we show that Hsl1 and Hsl7 are required for proper localization of Cdc5 (Polo-like kinase homolog) to the bud neck and Cdc5-dependent Swe1 phosphorylation...
  19. Rahal R, Amon A. The Polo-like kinase Cdc5 interacts with FEAR network components and Cdc14. Cell Cycle. 2008;7:3262-72 pubmed
    ..Here we show that the FEAR network component Cdc5 physically associates with two other members of the pathway, the Separase Esp1 and the Esp1-binding protein Slk19...
  20. Liang F, Jin F, Liu H, Wang Y. The molecular function of the yeast polo-like kinase Cdc5 in Cdc14 release during early anaphase. Mol Biol Cell. 2009;20:3671-9 pubmed publisher
    ..However, the function of another FEAR component, the Polo-like kinase Cdc5, remains elusive...
  21. Darieva Z, Bulmer R, Pic Taylor A, Doris K, Geymonat M, Sedgwick S, et al. Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Nature. 2006;444:494-8 pubmed
    ..Thus, polo kinase is required for the production of key mitotic regulators, in addition to previously defined roles in controlling other mitotic events. ..
  22. Park C, Song S, Lee P, Shou W, Deshaies R, Lee K. Loss of CDC5 function in Saccharomyces cerevisiae leads to defects in Swe1p regulation and Bfa1p/Bub2p-independent cytokinesis. Genetics. 2003;163:21-33 pubmed
    ..provide new insights into the function of budding yeast polo kinase Cdc5p, we generated novel temperature-sensitive cdc5 mutants by mutagenizing the C-terminal domain...
  23. Visintin R, Hwang E, Amon A. Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. Nature. 1999;398:818-23 pubmed
    ..A highly conserved signalling cascade, critical for the exit from mitosis, is required for this movement of Cdc14 during anaphase. Furthermore, we have identified a negative regulator of Cdc14, Cfi1, that anchors Cdc14 in the nucleolus. ..
  24. Luca F, Winey M. MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidy. Mol Biol Cell. 1998;9:29-46 pubmed
    ..MOB1 exhibits genetic interaction with three other yeast genes required for the completion of mitosis, LTE1, CDC5, and CDC15 (the latter two encode essential protein kinases)...
  25. Kitada K, Johnson A, Johnston L, Sugino A. A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5. Mol Cell Biol. 1993;13:4445-57 pubmed
    ..Nucleotide sequence analysis of the suppressor gene, provisionally named MSD2, revealed an open reading frame encoding a protein with a calculated M(r) of 81,024, with amino acid sequence ..
  26. Alexandru G, Uhlmann F, Mechtler K, Poupart M, Nasmyth K. Phosphorylation of the cohesin subunit Scc1 by Polo/Cdc5 kinase regulates sister chromatid separation in yeast. Cell. 2001;105:459-72 pubmed
    ..We show here that the Polo/Cdc5 kinase phosphorylates serine residues adjacent to Scc1 cleavage sites and strongly enhances their cleavage...
  27. Cheng L, Hunke L, Hardy C. Cell cycle regulation of the Saccharomyces cerevisiae polo-like kinase cdc5p. Mol Cell Biol. 1998;18:7360-70 pubmed
    ..These results further link the actions of the APC to the completion of mitosis and suggest possible roles for Cdc5p during progression through and completion of mitosis. ..
  28. Sakchaisri K, Asano S, Yu L, Shulewitz M, Park C, Park J, et al. Coupling morphogenesis to mitotic entry. Proc Natl Acad Sci U S A. 2004;101:4124-9 pubmed
    ..Here, we show that two other kinases targeted sequentially to the neck, Cla4/PAK and Cdc5/Polo, are responsible for stepwise phosphorylation and down-regulation of Swe1...
  29. Kim J, Jang S, Song K. Different levels of Bfa1/Bub2 GAP activity are required to prevent mitotic exit of budding yeast depending on the type of perturbations. Mol Biol Cell. 2008;19:4328-40 pubmed publisher
    ..These findings demonstrate that there is a GAP-independent surveillance mechanism of Bfa1/Bub2, which, together with the GTP/GDP switch of Tem1, may be required for the genomic stability of cells with misaligned spindles. ..
  30. Visintin R, Craig K, Hwang E, Prinz S, Tyers M, Amon A. The phosphatase Cdc14 triggers mitotic exit by reversal of Cdk-dependent phosphorylation. Mol Cell. 1998;2:709-18 pubmed
    ..Feedback between these pathways may lead to precipitous collapse of mitotic CDK activity and help coordinate exit from mitosis. ..
  31. Hu F, Wang Y, Liu D, Li Y, Qin J, Elledge S. Regulation of the Bub2/Bfa1 GAP complex by Cdc5 and cell cycle checkpoints. Cell. 2001;107:655-65 pubmed
    ..How the Bfa1/Bub2 complex is regulated is not well understood. We find that Polo/Cdc5 kinase acts upstream of Bfa1/Bub2 in the mitotic exit network...
  32. Clyne R, Katis V, Jessop L, Benjamin K, Herskowitz I, Lichten M, et al. Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I. Nat Cell Biol. 2003;5:480-5 pubmed
    ..is regulated by Polo-like kinases prompted us to investigate the meiotic role of the yeast Polo-like kinase Cdc5. We show here that cells lacking Cdc5 synapse homologues and initiate recombination normally, but fail to ..
  33. Mortensen E, Haas W, Gygi M, Gygi S, Kellogg D. Cdc28-dependent regulation of the Cdc5/Polo kinase. Curr Biol. 2005;15:2033-7 pubmed
    ..The budding yeast homologs of Cdk1 and Polo kinase are referred to as Cdc28 and Cdc5. We show that signaling from Cdc28 is required to maintain Cdc5 activity in vivo...
  34. Miller C, Gabrielse C, Chen Y, Weinreich M. Cdc7p-Dbf4p regulates mitotic exit by inhibiting Polo kinase. PLoS Genet. 2009;5:e1000498 pubmed publisher
    ..Therefore, Cdc7p-Dbf4p prevents inappropriate exit from mitosis by inhibiting Polo kinase and functions in the spindle position checkpoint. ..
  35. Visintin R, Amon A. Regulation of the mitotic exit protein kinases Cdc15 and Dbf2. Mol Biol Cell. 2001;12:2961-74 pubmed
    ..BUB2 negatively regulates all 3 events. Loading of Cdc15 on SPBs depends on TEM1, whereas loading of Dbf2 on SPBs and activation of Dbf2 kinase depend on TEM1 and CDC15. ..
  36. Charles J, Jaspersen S, Tinker Kulberg R, Hwang L, Szidon A, Morgan D. The Polo-related kinase Cdc5 activates and is destroyed by the mitotic cyclin destruction machinery in S. cerevisiae. Curr Biol. 1998;8:497-507 pubmed
    ..The Polo-related protein kinase Cdc5 in Saccharomyces cerevisiae might encode a regulator of the APC, because cdc5 mutant cells arrest with a late ..
  37. Toczyski D, Galgoczy D, Hartwell L. CDC5 and CKII control adaptation to the yeast DNA damage checkpoint. Cell. 1997;90:1097-106 pubmed
    ..We show that one mutation resides in CDC5, which encodes a polo-like kinase, whereas a second, less penetrant, adaptation-defective mutant is affected at the ..
  38. Hardy C, Pautz A. A novel role for Cdc5p in DNA replication. Mol Cell Biol. 1996;16:6775-82 pubmed
    ..Cdc5p and Cdc7p each interact with a distinct domain of Dbf4p. cdc5-1 mutants have a plasmid maintenance defect that can be suppressed by the addition of multiple origins...
  39. Fraschini R, D Ambrosio C, Venturetti M, Lucchini G, Piatti S. Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit. J Cell Biol. 2006;172:335-46 pubmed
    ..Moreover, it correlates with the passage of one spindle pole through the bud neck because it needs septin ring formation and bud neck kinases. ..
  40. Shou W, Azzam R, Chen S, Huddleston M, Baskerville C, Charbonneau H, et al. Cdc5 influences phosphorylation of Net1 and disassembly of the RENT complex. BMC Mol Biol. 2002;3:3 pubmed
    In S. cerevisiae, the mitotic exit network (MEN) proteins, including the Polo-like protein kinase Cdc5 and the protein phosphatase Cdc14, are required for exit from mitosis...
  41. Meitinger F, Petrova B, Lombardi I, Bertazzi D, Hub B, Zentgraf H, et al. Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast. J Cell Sci. 2010;123:1851-61 pubmed publisher
    ..This defect was partially suppressed by increased levels of Cyk3. We therefore propose that the MEN directly controls cytokinesis via targeting of Inn1, Cyk3 and Chs2 to the bud neck...
  42. Park J, Park C, Sakchaisri K, Karpova T, Asano S, McNally J, et al. Novel functional dissection of the localization-specific roles of budding yeast polo kinase Cdc5p. Mol Cell Biol. 2004;24:9873-86 pubmed
    ..Thus, SPB- and the bud-neck-localized Cdc5p control most of the critical Cdc5p functions and downregulation of Bfa1p and Swe1p at the respective locations are two critical factors that require Cdc5p. ..
  43. Pellicioli A, Lee S, Lucca C, Foiani M, Haber J. Regulation of Saccharomyces Rad53 checkpoint kinase during adaptation from DNA damage-induced G2/M arrest. Mol Cell. 2001;7:293-300 pubmed
    ..Rad53p kinase remains elevated in yku70delta and cdc5-ad cells that fail to adapt...
  44. Crasta K, Lim H, Giddings T, Winey M, Surana U. Inactivation of Cdh1 by synergistic action of Cdk1 and polo kinase is necessary for proper assembly of the mitotic spindle. Nat Cell Biol. 2008;10:665-75 pubmed publisher
    ..Thus, Cdh1 inactivation through the synergistic action of Cdk1 and polo kinase provides a new model for inactivation of cell-cycle effectors. ..
  45. Sourirajan A, Lichten M. Polo-like kinase Cdc5 drives exit from pachytene during budding yeast meiosis. Genes Dev. 2008;22:2627-32 pubmed publisher
    ..Ndt80 is required for meiotic function of polo-like kinase (PLK, Cdc5) and cyclin-dependent kinase (CDK), two cell cycle kinases previously implicated in pachytene exit...
  46. Fraschini R, Bilotta D, Lucchini G, Piatti S. Functional characterization of Dma1 and Dma2, the budding yeast homologues of Schizosaccharomyces pombe Dma1 and human Chfr. Mol Biol Cell. 2004;15:3796-810 pubmed
    ..Although their primary functions remain to be defined, our data suggest that Dma1 and Dma2 might be required to ensure timely MEN activation in telophase. ..
  47. Lee B, Amon A. Role of Polo-like kinase CDC5 in programming meiosis I chromosome segregation. Science. 2003;300:482-6 pubmed
    ..The budding yeast Polo-like kinase Cdc5 was found to be instrumental in establishing the meiosis I chromosome segregation program...
  48. Pereira G, Schiebel E. Kin4 kinase delays mitotic exit in response to spindle alignment defects. Mol Cell. 2005;19:209-21 pubmed
    ..Bub2-Bfa1 then becomes inhibited by Cdc5 polo kinase with anaphase onset, a prerequisite for mitotic exit...
  49. Höfken T, Schiebel E. Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2. J Cell Biol. 2004;164:219-31 pubmed
    ..Gic proteins became essential for mitotic exit when activation of the mitotic exit network through Cdc5 polo kinase and the bud cortex protein Lte1 was impaired...
  50. Schleker T, Shimada K, Sack R, Pike B, Gasser S. Cell cycle-dependent phosphorylation of Rad53 kinase by Cdc5 and Cdc28 modulates checkpoint adaptation. Cell Cycle. 2010;9:350-63 pubmed
    ..We show that Rad53 is phosphorylated in vitro by Cdc5, a mitotic Polo-like kinase, and by the yeast cyclin-dependent kinase, Cdc28...
  51. Jaspersen S, Charles J, Tinker Kulberg R, Morgan D. A late mitotic regulatory network controlling cyclin destruction in Saccharomyces cerevisiae. Mol Biol Cell. 1998;9:2803-17 pubmed
    ..In the budding yeast Saccharomyces cerevisiae, members of a large class of late mitotic mutants, including cdc15, cdc5, cdc14, dbf2, and tem1, arrest in anaphase with a phenotype similar to that of cells expressing nondegradable forms ..
  52. Hornig N, Uhlmann F. Preferential cleavage of chromatin-bound cohesin after targeted phosphorylation by Polo-like kinase. EMBO J. 2004;23:3144-53 pubmed
    ..Polo is bound to chromosomes independently of cohesin's presence, providing a possible explanation for chromosome-specific cohesin modification and targeting of separase cleavage. ..
  53. Rancati G, Crispo V, Lucchini G, Piatti S. Mad3/BubR1 phosphorylation during spindle checkpoint activation depends on both Polo and Aurora kinases in budding yeast. Cell Cycle. 2005;4:972-80 pubmed
    ..In vivo Mad3 phosphorylation depends on the Polo kinase Cdc5 and, to a minor extent, the Aurora B kinase Ipl1...
  54. Matos J, Blanco M, West S. Cell-cycle kinases coordinate the resolution of recombination intermediates with chromosome segregation. Cell Rep. 2013;4:76-86 pubmed publisher
    ..Here, we have analyzed the role of the cell-cycle kinases Cdk and Cdc5 in coordinating these events through their involvement in the phosphoregulation of the Mus81-Mms4 nuclease...