CDC42

Summary

Gene Symbol: CDC42
Description: Rho family GTPase CDC42
Alias: Rho family GTPase CDC42
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Nern A, Arkowitz R. A GTP-exchange factor required for cell orientation. Nature. 1998;391:195-8 pubmed
    ..The Rho-family GTPase Cdc42 and its exchange factor Cdc24 have been implicated in the mating process, but their specific role is unknown...
  2. Tcheperegine S, Gao X, Bi E. Regulation of cell polarity by interactions of Msb3 and Msb4 with Cdc42 and polarisome components. Mol Cell Biol. 2005;25:8567-80 pubmed
    ..We also show that Msb3 and Msb4 bind specifically to Cdc42-GDP and Rho1-GDP in vitro and that Msb3 and Rho GDP dissociation inhibitor act independently but oppositely on ..
  3. Madden K, Snyder M. Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol. 1998;52:687-744 pubmed
    ..Since many of the components important for polarized cell growth are conserved in other organisms, the basic mechanisms mediating polarized cell growth are likely to be universal among eukaryotes. ..
  4. Bender L, Lo H, Lee H, Kokojan V, Peterson V, Bender A. Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast. J Cell Biol. 1996;133:879-94 pubmed
  5. Bender A, Pringle J. Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Proc Natl Acad Sci U S A. 1989;86:9976-80 pubmed
    Genes CDC24, CDC42, and CDC43 are required for the establishment of cell polarity and the localization of secretion in Saccharomyces cerevisiae; mutants defective in these genes fail to form buds and display isotropic expansion of the ..
  6. Atkins B, Yoshida S, Saito K, Wu C, Lew D, Pellman D. Inhibition of Cdc42 during mitotic exit is required for cytokinesis. J Cell Biol. 2013;202:231-40 pubmed publisher
    The role of Cdc42 and its regulation during cytokinesis is not well understood...
  7. Smith G, Givan S, Cullen P, Sprague G. GTPase-activating proteins for Cdc42. Eukaryot Cell. 2002;1:469-80 pubmed
    The Rho-type GTPase, Cdc42, has been implicated in a variety of functions in the yeast life cycle, including septin organization for cytokinesis, pheromone response, and haploid invasive growth...
  8. Cole K, McLaughlin H, Johnson D. Use of bimolecular fluorescence complementation to study in vivo interactions between Cdc42p and Rdi1p of Saccharomyces cerevisiae. Eukaryot Cell. 2007;6:378-87 pubmed
    ..Data from these studies have facilitated the temporal and spatial modeling of Rdi1p-dependent extraction of Cdc42p from the plasma membrane during the cell cycle. ..
  9. Goryachev A, Pokhilko A. Computational model explains high activity and rapid cycling of Rho GTPases within protein complexes. PLoS Comput Biol. 2006;2:e172 pubmed
    ..Interestingly, we find that the cycling regimes are only weakly dependent on the concentration of GTPase itself. ..

More Information

Publications76

  1. Smith S, Rubinstein B, Mendes Pinto I, Slaughter B, Unruh J, Li R. Independence of symmetry breaking on Bem1-mediated autocatalytic activation of Cdc42. J Cell Biol. 2013;202:1091-106 pubmed publisher
    ..via a positive feedback loop, wherein the adaptor protein Bem1 promotes local activation and accumulation of Cdc42 by directly tethering Cdc42(GTP) with its guanine nucleotide exchange factor (GEF) Cdc24...
  2. Leberer E, Chenevert J, Leeuw T, Harcus D, Herskowitz I, Thomas D. Genetic interactions indicate a role for Mdg1p and the SH3 domain protein Bem1p in linking the G-protein mediated yeast pheromone signalling pathway to regulators of cell polarity. Mol Gen Genet. 1996;252:608-21 pubmed
    ..The mating defect of cells deleted for STE20 is partially suppressed by multiple copies of BEM1 and CDC42, which encodes a small GTP-binding protein that binds to Ste20p and is necessary for the development of cell ..
  3. Heinrich M, Köhler T, Mösch H. Role of Cdc42-Cla4 interaction in the pheromone response of Saccharomyces cerevisiae. Eukaryot Cell. 2007;6:317-27 pubmed
    In Saccharomyces cerevisiae, the highly conserved Rho-type GTPase Cdc42 is essential for cell division and controls cellular development during mating and invasive growth...
  4. Davis C, Richman T, Deliduka S, Blaisdell J, Collins C, Johnson D. Analysis of the mechanisms of action of the Saccharomyces cerevisiae dominant lethal cdc42G12V and dominant negative cdc42D118A mutations. J Biol Chem. 1998;273:849-58 pubmed
    ..The mechanisms of action of the dominant negative cdc42(D118A) mutant and the lethal, gain of function cdc42(G12V) mutant were examined...
  5. Butty A, Perrinjaquet N, Petit A, Jaquenoud M, Segall J, Hofmann K, et al. A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization. EMBO J. 2002;21:1565-76 pubmed
    In Saccharomyces cerevisiae, activation of Cdc42 by its guanine-nucleotide exchange factor Cdc24 triggers polarization of the actin cytoskeleton at bud emergence and in response to mating pheromones...
  6. Kozminski K, Alfaro G, Dighe S, Beh C. Homologues of oxysterol-binding proteins affect Cdc42p- and Rho1p-mediated cell polarization in Saccharomyces cerevisiae. Traffic. 2006;7:1224-42 pubmed
    ..In a screen for Saccharomyces cerevisiae genes that interact with Rho family CDC42 to promote polarity establishment, we identified KES1/OSH4, which encodes a homologue of mammalian oxysterol-..
  7. Gao X, Albert S, Tcheperegine S, Burd C, Gallwitz D, Bi E. The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J Cell Biol. 2003;162:635-46 pubmed
    ..for efficient polarization of the actin patches and for the suppression of the actin-organization defects in cdc42 mutants...
  8. Gladfelter A, Moskow J, Zyla T, Lew D. Isolation and characterization of effector-loop mutants of CDC42 in yeast. Mol Biol Cell. 2001;12:1239-55 pubmed
    ..the generation and preliminary characterization of a set of effector-loop mutants of Saccharomyces cerevisiae CDC42. These mutants define genetically separable pathways influencing actin or septin organization...
  9. Mösch H, Kohler T, Braus G. Different domains of the essential GTPase Cdc42p required for growth and development of Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:235-48 pubmed
  10. Richman T, Sawyer M, Johnson D. Saccharomyces cerevisiae Cdc42p localizes to cellular membranes and clusters at sites of polarized growth. Eukaryot Cell. 2002;1:458-68 pubmed
    ..To examine the cell cycle-specific targeting of Cdc42p in living yeast cells, a green fluorescent protein (GFP)-Cdc42 fusion protein was used...
  11. Matsui Y, Toh e A. Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1. Mol Cell Biol. 1992;12:5690-9 pubmed
    ..Two of them were identical with CDC42 and BEM1, bud site assembly genes involved in the process of bud emergence...
  12. Osman M, Cerione R. Iqg1p, a yeast homologue of the mammalian IQGAPs, mediates cdc42p effects on the actin cytoskeleton. J Cell Biol. 1998;142:443-55 pubmed
    ..Mol. Cell. Biol. 15:723-730). We propose that Iqg1p acts as a scaffold to recruit and localize a protein complex involved in actin-based cellular functions and thus mediates the regulatory effects of Cdc42p on the actin cytoskeleton. ..
  13. Knaus M, Pelli Gulli M, van Drogen F, Springer S, Jaquenoud M, Peter M. Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence. EMBO J. 2007;26:4501-13 pubmed
  14. Ziman M, Preuss D, Mulholland J, O Brien J, Botstein D, Johnson D. Subcellular localization of Cdc42p, a Saccharomyces cerevisiae GTP-binding protein involved in the control of cell polarity. Mol Biol Cell. 1993;4:1307-16 pubmed
    The Saccharomyces cerevisiae Cdc42 protein, a member of the Ras superfamily of low-molecular-weight GTP-binding proteins, is involved in the control of cell polarity during the yeast cell cycle...
  15. Caviston J, Longtine M, Pringle J, Bi E. The role of Cdc42p GTPase-activating proteins in assembly of the septin ring in yeast. Mol Biol Cell. 2003;14:4051-66 pubmed
  16. Richman T, Toenjes K, Morales S, Cole K, Wasserman B, Taylor C, et al. Analysis of cell-cycle specific localization of the Rdi1p RhoGDI and the structural determinants required for Cdc42p membrane localization and clustering at sites of polarized growth. Curr Genet. 2004;45:339-49 pubmed
    ..Taken together, these results provide insight into the complicated nature of the relationships between Cdc42p localization, nucleotide binding, and protein-protein interactions. ..
  17. Yamaguchi Y, Ota K, Ito T. A novel Cdc42-interacting domain of the yeast polarity establishment protein Bem1. Implications for modulation of mating pheromone signaling. J Biol Chem. 2007;282:29-38 pubmed
    In Saccharomyces cerevisiae, the Rho-type small GTPase Cdc42 is activated by its guanine-nucleotide exchange factor Cdc24 to polarize the cell for budding and mating...
  18. Zhang X, Bi E, Novick P, Du L, Kozminski K, Lipschutz J, et al. Cdc42 interacts with the exocyst and regulates polarized secretion. J Biol Chem. 2001;276:46745-50 pubmed
    ..that regulate the localization of the exocyst in the budding yeast Saccharomyces cerevisiae, we found that certain cdc42 mutants affect the polarized localization of the exocyst proteins...
  19. Alfaro G, Johansen J, Dighe S, Duamel G, Kozminski K, Beh C. The sterol-binding protein Kes1/Osh4p is a regulator of polarized exocytosis. Traffic. 2011;12:1521-36 pubmed publisher
    ..These results support a model in which Osh4p acts as a sterol-dependent regulator of polarized vesicle transport, as opposed to being a sterol-transfer protein. ..
  20. Richman T, Johnson D. Saccharomyces cerevisiae cdc42p GTPase is involved in preventing the recurrence of bud emergence during the cell cycle. Mol Cell Biol. 2000;20:8548-59 pubmed
    ..Specifically, Cdc42(D38E)p showed reduced interactions with the Cla4p p21-activated protein kinase and the Bem3p GTPase-activating ..
  21. Zhao Z, Leung T, Manser E, Lim L. Pheromone signalling in Saccharomyces cerevisiae requires the small GTP-binding protein Cdc42p and its activator CDC24. Mol Cell Biol. 1995;15:5246-57 pubmed
    ..subunits is Ste20p, whose structural homolog, the serine/threonine kinase PAK, is activated by GTP-binding p21s Cdc42 and Rac1...
  22. Brown J, Jaquenoud M, Gulli M, Chant J, Peter M. Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast. Genes Dev. 1997;11:2972-82 pubmed
    ..yeast genomic sequence, we have identified two novel Cdc42p targets, Gic1p and Gic2p, which contain consensus Cdc42/Rac interactive-binding (CRIB) domains and bind specifically to Cdc42p-GTP...
  23. Kuo C, Savage N, Chen H, Wu C, Zyla T, Lew D. Inhibitory GEF phosphorylation provides negative feedback in the yeast polarity circuit. Curr Biol. 2014;24:753-9 pubmed publisher
    ..The front accumulates high levels of the active form of a polarity-determining Rho-family GTPase (Cdc42, Rac, or Rop) that then orients cytoskeletal elements through various effectors to generate the polarized ..
  24. Chen G, Zheng L, Chan C. The LIM domain-containing Dbm1 GTPase-activating protein is required for normal cellular morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:1376-90 pubmed
  25. Gao X, Caviston J, Tcheperegine S, Bi E. Pxl1p, a paxillin-like protein in Saccharomyces cerevisiae, may coordinate Cdc42p and Rho1p functions during polarized growth. Mol Biol Cell. 2004;15:3977-85 pubmed
    ..Based on its ability to suppress cdc42-Ts mutants when overexpressed, a novel gene PXL1 was identified...
  26. Roumanie O, Wu H, Molk J, Rossi G, Bloom K, Brennwald P. Rho GTPase regulation of exocytosis in yeast is independent of GTP hydrolysis and polarization of the exocyst complex. J Cell Biol. 2005;170:583-94 pubmed
    ..This suggests that Rho3 and Cdc42 act to polarize exocytosis by activating the exocytic machinery at the membrane without the need to first recruit ..
  27. Tjandra H, Compton J, Kellogg D. Control of mitotic events by the Cdc42 GTPase, the Clb2 cyclin and a member of the PAK kinase family. Curr Biol. 1998;8:991-1000 pubmed
    ..We have found that the Cdc42 GTPase and a member of the PAK kinase family called Cla4 both function in the pathway used by Clb2 to control bud ..
  28. Okada S, Leda M, Hanna J, Savage N, Bi E, Goryachev A. Daughter cell identity emerges from the interplay of Cdc42, septins, and exocytosis. Dev Cell. 2013;26:148-61 pubmed publisher
    ..Here, we show that septins recruited to the site of polarization by Cdc42-GTP inhibit Cdc42 activity in a negative feedback loop, and this inhibition depends on Cdc42 GTPase-activating ..
  29. Höfken T, Schiebel E. A role for cell polarity proteins in mitotic exit. EMBO J. 2002;21:4851-62 pubmed
    ..The Rho-like GTPase Cdc42, its GEF Cdc24 and its effector Cla4 [a member of the p21-activated kinases (PAKs)] control the initial binding ..
  30. Yamamoto T, Mochida J, Kadota J, Takeda M, Bi E, Tanaka K. Initial polarized bud growth by endocytic recycling in the absence of actin cable-dependent vesicle transport in yeast. Mol Biol Cell. 2010;21:1237-52 pubmed publisher
  31. Kang P, Beven L, Hariharan S, Park H. The Rsr1/Bud1 GTPase interacts with itself and the Cdc42 GTPase during bud-site selection and polarity establishment in budding yeast. Mol Biol Cell. 2010;21:3007-16 pubmed publisher
    ..Rsr1 also exhibits heterotypic interaction with the Cdc42 GTPase in vivo...
  32. Das A, Slaughter B, Unruh J, Bradford W, Alexander R, Rubinstein B, et al. Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity. Nat Cell Biol. 2012;14:304-10 pubmed publisher
    ..we find that a lipid flippase complex, composed of Lem3, Dnf1 or Dnf2, has a role in the dynamic recycling of the Cdc42 GTPase, a key regulator of cell polarity, in yeast...
  33. Finger F, Novick P. Synthetic interactions of the post-Golgi sec mutations of Saccharomyces cerevisiae. Genetics. 2000;156:943-51 pubmed
    ..The significance of these results is discussed in the context of both secretory pathway function and the utility of synthetic lethality studies and their interpretation. ..
  34. Richman T, Sawyer M, Johnson D. The Cdc42p GTPase is involved in a G2/M morphogenetic checkpoint regulating the apical-isotropic switch and nuclear division in yeast. J Biol Chem. 1999;274:16861-70 pubmed
    ..cerevisiae. Cells expressing the cdc42(V44A) effector domain mutant allele displayed morphological defects of highly elongated and multielongated budded ..
  35. Simon M, De Virgilio C, Souza B, Pringle J, Abo A, Reed S. Role for the Rho-family GTPase Cdc42 in yeast mating-pheromone signal pathway. Nature. 1995;376:702-5 pubmed
    ..Temperature-sensitive cells with mutations of the CDC24 and CDC42 genes, which are incapable of budding and of generating cell polarity at the restrictive temperature, are also ..
  36. Tong Z, Gao X, Howell A, Bose I, Lew D, Bi E. Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition. J Cell Biol. 2007;179:1375-84 pubmed publisher
    ..division, to which they recruit Cdc24, the guanine nucleotide exchange factor for the conserved polarity regulator Cdc42. However, instead of polarizing at the division site, the new polarity axis is directed next to but not ..
  37. Takahashi S, Pryciak P. Identification of novel membrane-binding domains in multiple yeast Cdc42 effectors. Mol Biol Cell. 2007;18:4945-56 pubmed
    The Rho-type GTPase Cdc42 is a central regulator of eukaryotic cell polarity and signal transduction...
  38. Gao X, Sperber L, Kane S, Tong Z, Tong A, Boone C, et al. Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle. Mol Biol Cell. 2007;18:2542-60 pubmed
    ..Together, these results suggest that Axl2p plays sequential and distinct roles in the regulation of cellular morphogenesis in yeast cell cycle. ..
  39. Lamson R, Winters M, Pryciak P. Cdc42 regulation of kinase activity and signaling by the yeast p21-activated kinase Ste20. Mol Cell Biol. 2002;22:2939-51 pubmed
    ..While PAKs are usually activated by small G proteins and Ste20 binds Cdc42, the role of Cdc42-Ste20 binding has been controversial, largely because Ste20 lacking its entire Cdc42-binding (..
  40. Höfken T, Schiebel E. Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2. J Cell Biol. 2004;164:219-31 pubmed
    The guanine nucleotide exchange factor Cdc24, the GTPase Cdc42, and the Cdc42 effectors Cla4 and Ste20, two p21-activated kinases, form a signal transduction cascade that promotes mitotic exit in yeast...
  41. Evangelista M, Blundell K, Longtine M, Chow C, Adames N, Pringle J, et al. Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis. Science. 1997;276:118-22 pubmed
    ..Both Bni1p and Bud6p (like Cdc42p and actin) localized to the tips of mating projections. Bni1p may function as a Cdc42p target that links the pheromone response pathway to the actin cytoskeleton. ..
  42. Adamo J, Moskow J, Gladfelter A, Viterbo D, Lew D, Brennwald P. Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud. J Cell Biol. 2001;155:581-92 pubmed
    The Rho family GTPase Cdc42 is a key regulator of cell polarity and cytoskeletal organization in eukaryotic cells...
  43. Moskow J, Gladfelter A, Lamson R, Pryciak P, Lew D. Role of Cdc42p in pheromone-stimulated signal transduction in Saccharomyces cerevisiae. Mol Cell Biol. 2000;20:7559-71 pubmed
    b>CDC42 encodes a highly conserved GTPase of the Rho family that is best known for its role in regulating cell polarity and actin organization...
  44. Koch G, Tanaka K, Masuda T, Yamochi W, Nonaka H, Takai Y. Association of the Rho family small GTP-binding proteins with Rho GDP dissociation inhibitor (Rho GDI) in Saccharomyces cerevisiae. Oncogene. 1997;15:417-22 pubmed
    The small GTP-binding proteins of the Rho family, consisting of the Rho, Rac, and Cdc42 subfamilies, are implicated in various cell functions, such as cell shape change, cell motility and cytokinesis, through reorganization of actin ..
  45. Slaughter B, Das A, Schwartz J, Rubinstein B, Li R. Dual modes of cdc42 recycling fine-tune polarized morphogenesis. Dev Cell. 2009;17:823-35 pubmed publisher
    In budding yeast, the highly conserved small GTPase Cdc42 localizes to the cortex at a cell pole and orchestrates the trafficking and deposition of cell surface materials required for building a bud or mating projection (shmoo)...
  46. Moffat J, Andrews B. Late-G1 cyclin-CDK activity is essential for control of cell morphogenesis in budding yeast. Nat Cell Biol. 2004;6:59-66 pubmed
    ..polarized cell growth, and strains lacking PCL1 and PCL2 show genetic interactions with the cell polarity GTPase Cdc42, its regulators and downstream effectors...
  47. Bi E, Pringle J. ZDS1 and ZDS2, genes whose products may regulate Cdc42p in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:5264-75 pubmed
    ..As ZDS1 and ZDS2 have recently been identified also by numerous other groups studying a wide range of biological phenomena, the roles of Cdc42p in intracellular signaling may be more diverse than has previously been appreciated. ..
  48. Benton B, Tinkelenberg A, Gonzalez I, Cross F. Cla4p, a Saccharomyces cerevisiae Cdc42p-activated kinase involved in cytokinesis, is activated at mitosis. Mol Cell Biol. 1997;17:5067-76 pubmed
    ..Cla4p is homologous to mammalian p21-activated kinases (PAKs) (kinases activated by the rho-class GTPase Rac or Cdc42). We developed a kinase assay for Cla4p. Cla4p kinase was activated in vivo by the GTP-bound form of Cdc42p...
  49. Gong T, Liao Y, He F, Yang Y, Yang D, Chen X, et al. Control of polarized growth by the Rho family GTPase Rho4 in budding yeast: requirement of the N-terminal extension of Rho4 and regulation by the Rho GTPase-activating protein Bem2. Eukaryot Cell. 2013;12:368-77 pubmed publisher
    ..Furthermore, we show that Rho4 interacts with Bem2, a Rho GTPase-activating protein (RhoGAP) for Cdc42 and Rho1, by yeast two-hybrid, bimolecular fluorescence complementation (BiFC), and glutathione S-transferase (GST)..
  50. Bi E, Chiavetta J, Chen H, Chen G, Chan C, Pringle J. Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast. Mol Biol Cell. 2000;11:773-93 pubmed
    ..that is distinct from its Cdc42p guanine-nucleotide-exchange factor activity; in particular, overexpression of CDC42 in combination with MSB1 or a truncated CLA4 in cells depleted for Cdc24p allowed polarization of the actin ..
  51. Bender A, Pringle J. Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol. 1991;11:1295-305 pubmed
    Genes CDC24 and CDC42 are required for the establishment of cell polarity and for bud formation in Saccharomyces cerevisiae...
  52. Iwase M, Luo J, Nagaraj S, Longtine M, Kim H, Haarer B, et al. Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Mol Biol Cell. 2006;17:1110-25 pubmed
    ..Two-hybrid, in vitro protein binding, and coimmunoprecipitation data indicate that this role involves a direct interaction of the Gic proteins with the septin Cdc12p. ..
  53. Cid V, Adamíková L, Sanchez M, Molina M, Nombela C. Cell cycle control of septin ring dynamics in the budding yeast. Microbiology. 2001;147:1437-50 pubmed
    ..we show that septin assembly is dependent on G1 cyclin/Cdc28-mediated cell cycle signals and that the small GTPase Cdc42, but not Rho1, are essential for this event...
  54. Gulli M, Jaquenoud M, Shimada Y, Niederhauser G, Wiget P, Peter M. Phosphorylation of the Cdc42 exchange factor Cdc24 by the PAK-like kinase Cla4 may regulate polarized growth in yeast. Mol Cell. 2000;6:1155-67 pubmed
    ..cerevisiae, activation of the CDK Cdc28-Cln2 at bud emergence triggers relocalization of Cdc24, the GEF for Cdc42, from the nucleus to the polarization site, where it is stably maintained by binding to the adaptor Bem1...
  55. Zheng Y, Cerione R, Bender A. Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3. J Biol Chem. 1994;269:2369-72 pubmed
    ..reorganizations of cortical cytoskeletal elements and requires the action of the Rho (Ras homologous)-type GTPase Cdc42. As a first step toward understanding how these cytoskeletal rearrangements are controlled, we have sought to ..
  56. Ozbudak E, Becskei A, van Oudenaarden A. A system of counteracting feedback loops regulates Cdc42p activity during spontaneous cell polarization. Dev Cell. 2005;9:565-71 pubmed
  57. Zhang X, Orlando K, He B, Xi F, Zhang J, Zajac A, et al. Membrane association and functional regulation of Sec3 by phospholipids and Cdc42. J Cell Biol. 2008;180:145-58 pubmed publisher
    ..have identified key residues in Sec3 that are critical for its binding to the guanosine triphosphate-bound form of Cdc42. Genetic analyses indicate that the dual interactions of Sec3 with phospholipids and Cdc42 control its function in ..
  58. Kozubowski L, Saito K, Johnson J, Howell A, Zyla T, Lew D. Symmetry-breaking polarization driven by a Cdc42p GEF-PAK complex. Curr Biol. 2008;18:1719-26 pubmed publisher
    ..Our findings provide mechanistic insight into an evolutionarily conserved pattern-forming positive-feedback pathway. ..
  59. Jaquenoud M, Peter M. Gic2p may link activated Cdc42p to components involved in actin polarization, including Bni1p and Bud6p (Aip3p). Mol Cell Biol. 2000;20:6244-58 pubmed
    ..We propose that at bud emergence, Gic2p functions as an adaptor which may link activated Cdc42p to components involved in actin organization and polarized growth, including Bni1p, Spa2p, and Bud6p. ..
  60. Meitinger F, Richter H, Heisel S, Hub B, Seufert W, Pereira G. A safeguard mechanism regulates Rho GTPases to coordinate cytokinesis with the establishment of cell polarity. PLoS Biol. 2013;11:e1001495 pubmed publisher
    ..1) establishes a novel polarity cue that concomitantly sustains Rho1-dependent polarization and inhibits premature Cdc42 activation at the site of cytokinesis...
  61. Zheng Y, Bender A, Cerione R. Interactions among proteins involved in bud-site selection and bud-site assembly in Saccharomyces cerevisiae. J Biol Chem. 1995;270:626-30 pubmed
    ..of the Ras-type GTPase Rsr1, which is required for the proper selection of the bud site, and the Rho-type GTPase Cdc42, which is necessary for the assembly of cytoskeletal structures at that site...
  62. Barale S, McCusker D, Arkowitz R. Cdc42p GDP/GTP cycling is necessary for efficient cell fusion during yeast mating. Mol Biol Cell. 2006;17:2824-38 pubmed
    ..study, we further investigated the role of Cdc42p in the mating process by screening for specific mating defective cdc42 alleles...
  63. Shimada Y, Wiget P, Gulli M, Bi E, Peter M. The nucleotide exchange factor Cdc24p may be regulated by auto-inhibition. EMBO J. 2004;23:1051-62 pubmed
    ..Taken together, our results support a two-step molecular mechanism for the site-specific activation of Cdc24p, which involves Rsr1p/Bud1p and the adaptor protein Bem1p. ..
  64. Marquitz A, Harrison J, Bose I, Zyla T, McMillan J, Lew D. The Rho-GAP Bem2p plays a GAP-independent role in the morphogenesis checkpoint. EMBO J. 2002;21:4012-25 pubmed
    ..Surprisingly, Swe1p accumulation occurred normally in bem2 cells, but they were nevertheless unable to promote Cdc28p phosphorylation. Therefore, Bem2p defines a novel pathway in the morphogenesis checkpoint. ..
  65. Mösch H, Kübler E, Krappmann S, Fink G, Braus G. Crosstalk between the Ras2p-controlled mitogen-activated protein kinase and cAMP pathways during invasive growth of Saccharomyces cerevisiae. Mol Biol Cell. 1999;10:1325-35 pubmed
  66. Chen G, Kim Y, Chan C. The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae. Genes Dev. 1997;11:2958-71 pubmed
    ..Each protein contains a CRIB (Cdc42/Rac-interactive binding) motif and each interacts in the two-hybrid assay with the GTP-bound form of the Rho-type ..
  67. Mack D, Nishimura K, Dennehey B, Arbogast T, Parkinson J, Toh e A, et al. Identification of the bud emergence gene BEM4 and its interactions with rho-type GTPases in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:4387-95 pubmed
    ..To identify genes whose functions are linked to CDC42, we screened for (i) multicopy suppressors of a Ts- cdc42 mutant, (ii) mutants that require multiple copies of ..