Gene Symbol: CDC40
Description: Cdc40p
Alias: PRP17, SLT15, SLU4, Cdc40p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Umen J, Guthrie C. Prp16p, Slu7p, and Prp8p interact with the 3' splice site in two distinct stages during the second catalytic step of pre-mRNA splicing. RNA. 1995;1:584-97 pubmed
    ..Therefore, Prp8p and Slu7p interact with the 3' splice site at the latest stage of splicing prior to the second catalytic step that can currently be defined, and may be at the active site. ..
  2. Kawashima T, Pellegrini M, Chanfreau G. Nonsense-mediated mRNA decay mutes the splicing defects of spliceosome component mutations. RNA. 2009;15:2236-47 pubmed publisher
  3. Query C, Konarska M. CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. RNA. 2012;18:1001-13 pubmed publisher
    ..caused by a variety of intron mutations, mutations in U6 snRNA, or deletion of the second-step protein factor Prp17, and they can activate alternative 3' splice sites...
  4. Lindsey Boltz L, Chawla G, Srinivasan N, Vijayraghavan U, Garcia Blanco M. The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function. RNA. 2000;6:1289-305 pubmed
    ..Temperature-sensitive prp17 alleles with mutations of these four amino acids are defective for the second step of splicing and are ..
  5. Kupiec M, Simchen G. DNA-repair characterization of cdc40-1, a cell-cycle mutant of Saccharomyces cerevisiae. Mutat Res. 1986;162:33-40 pubmed
    The cell-cycle specific mutation cdc40-1, which has been previously shown to be sensitive to MMS at the restrictive temperature, was further characterized as a DNA-repair-deficient mutation...
  6. Jones M, Frank D, Guthrie C. Characterization and functional ordering of Slu7p and Prp17p during the second step of pre-mRNA splicing in yeast. Proc Natl Acad Sci U S A. 1995;92:9687-91 pubmed
    Temperature-sensitive alleles in four genes (slu7-1, prp16-2, prp17-1, and prp18-1) are known to confer a specific block to the second chemical step of pre-mRNA splicing in vivo in the yeast Saccharomyces cerevisiae...
  7. Xu D, Field D, Tang S, Moris A, Bobechko B, Friesen J. Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol Cell Biol. 1998;18:2055-66 pubmed
    ..The remaining four slt mutations are new alleles of previously identified splicing genes: slt15, previously identified as prp17 (slt15/prp17-100), slt16/smd3-1, slt17/slu7-100, and slt21/prp8-21...
  8. Ben Yehuda S, Russell C, Dix I, Beggs J, Kupiec M. Extensive genetic interactions between PRP8 and PRP17/CDC40, two yeast genes involved in pre-mRNA splicing and cell cycle progression. Genetics. 2000;154:61-71 pubmed
    Biochemical and genetic experiments have shown that the PRP17 gene of the yeast Saccharomyces cerevisiae encodes a protein that plays a role during the second catalytic step of the splicing reaction...
  9. Kawashima T, Douglass S, Gabunilas J, Pellegrini M, Chanfreau G. Widespread use of non-productive alternative splice sites in Saccharomyces cerevisiae. PLoS Genet. 2014;10:e1004249 pubmed publisher
    ..cerevisiae but masked by RNA degradation and that the use of alternative splice sites in this organism is mostly aimed at controlling transcript levels rather than increasing proteome diversity. ..

More Information


  1. Ben Yehuda S, Dix I, Russell C, Levy S, Beggs J, Kupiec M. Identification and functional analysis of hPRP17, the human homologue of the PRP17/CDC40 yeast gene involved in splicing and cell cycle control. RNA. 1998;4:1304-12 pubmed
    The PRP17 gene of the yeast Saccharomyces cerevisiae encodes a protein that participates in the second step of the splicing reaction. It was found recently that the yeast PRP17 gene is identical to the cell division cycle CDC40 gene...
  2. Dagher S, Fu X. Evidence for a role of Sky1p-mediated phosphorylation in 3' splice site recognition involving both Prp8 and Prp17/Slu4. RNA. 2001;7:1284-97 pubmed
    ..Here we report that SKY1, a SRPK family member in Saccharomyces cerevisiae, genetically interacts with PRP8 and PRP17/SLU4, both of which are involved in splice site selection during pre-mRNA splicing...
  3. Vincent K, Wang Q, Jay S, Hobbs K, Rymond B. Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing. Genetics. 2003;164:895-907 pubmed
    ..e., clf1Delta2). The identified genes encode the Mud2, Ntc20, Prp16, Prp17, Prp19, Prp22, and Syf2 splicing factors and four proteins without established contribution to splicing (Bud13, ..
  4. Dahan O, Kupiec M. The Saccharomyces cerevisiae gene CDC40/PRP17 controls cell cycle progression through splicing of the ANC1 gene. Nucleic Acids Res. 2004;32:2529-40 pubmed
    ..point mutation analysis, specific residues in the ANC1 intron that are important for its splicing dependency on Cdc40p. Our results demonstrate a novel mechanism of cell cycle regulation that relies on the differential splicing of a ..
  5. Ben Yehuda S, Dix I, Russell C, McGarvey M, Beggs J, Kupiec M. Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae. Genetics. 2000;156:1503-17 pubmed
    The PRP17/CDC40 gene of Saccharomyces cerevisiae functions in two different cellular processes: pre-mRNA splicing and cell cycle progression...
  6. Chepurnaia O, Peshekhonov V, Kozhina T, Korolev V. [The xrs2 gene controls recombination repair in yeast]. Genetika. 1993;29:571-80 pubmed
    ..On the other hand, double mutants xrs2-6 and rad50-1 (cdc40-1, radH) have the same radiosensitivity as the single mutants...
  7. Sapra A, Khandelia P, Vijayraghavan U. The splicing factor Prp17 interacts with the U2, U5 and U6 snRNPs and associates with the spliceosome pre- and post-catalysis. Biochem J. 2008;416:365-74 pubmed publisher
    Saccharomyces cerevisiae PRP17-null mutants are temperature-sensitive for growth...
  8. Frank D, Patterson B, Guthrie C. Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing. Mol Cell Biol. 1992;12:5197-205 pubmed
    ..SLU1 and SLU2 are essential for the first catalytic step of splicing, while SLU7 and SLU4 (an allele of PRP17 [U. Vijayraghavan, M. Company, and J. Abelson, Genes Dev...
  9. Hackmann A, Wu H, Schneider U, Meyer K, Jung K, Krebber H. Quality control of spliced mRNAs requires the shuttling SR proteins Gbp2 and Hrb1. Nat Commun. 2014;5:3123 pubmed publisher
    ..Altogether, these data identify a role for shuttling SR proteins in mRNA surveillance and nuclear mRNA quality control. ..
  10. Burns C, Ohi R, Mehta S, O TOOLE E, Winey M, Clark T, et al. Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:801-15 pubmed
  11. Russell C, Ben Yehuda S, Dix I, Kupiec M, Beggs J. Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae. RNA. 2000;6:1565-72 pubmed
    Through a genetic screen to search for factors that interact with Prp17/Cdc40p, a protein involved in both cell cycle progression and pre-mRNA splicing, we identify three novel factors, which we call Syf1p, Syf2p, and Syf3 (SYnthetic ..
  12. Sapra A, Arava Y, Khandelia P, Vijayraghavan U. Genome-wide analysis of pre-mRNA splicing: intron features govern the requirement for the second-step factor, Prp17 in Saccharomyces cerevisiae and Schizosaccharomyces pombe. J Biol Chem. 2004;279:52437-46 pubmed
    ..Comparison of expression profiles, for intron-containing transcripts, among mutants of two second-step factors, Prp17 and Prp22, reveals their unique and shared effects on global splicing...
  13. Tomala K, Korona R. Alleviation of deleterious effects of protein mutation through inactivation of molecular chaperones. Mol Genet Genomics. 2008;280:409-17 pubmed publisher
    ..We suggest that under non-stressful conditions, molecular chaperones are tuned to support folding of native proteins, but not that of mutated ones. ..
  14. Chawla G, Sapra A, Surana U, Vijayraghavan U. Dependence of pre-mRNA introns on PRP17, a non-essential splicing factor: implications for efficient progression through cell cycle transitions. Nucleic Acids Res. 2003;31:2333-43 pubmed
    Saccharomyces cerevisiae PRP17 (CDC40) encodes a second-step pre-mRNA splicing factor with a role in cell division...
  15. Schuberth C, Buchberger A. Membrane-bound Ubx2 recruits Cdc48 to ubiquitin ligases and their substrates to ensure efficient ER-associated protein degradation. Nat Cell Biol. 2005;7:999-1006 pubmed
    ..Lack of Ubx2 causes defects in ERAD that are exacerbated under stress conditions. These findings are consistent with a model in which Ubx2 coordinates the assembly of a highly efficient ERAD machinery at the ER membrane. ..
  16. Mnaimneh S, Davierwala A, Haynes J, Moffat J, Peng W, Zhang W, et al. Exploration of essential gene functions via titratable promoter alleles. Cell. 2004;118:31-44 pubmed
    ..We furthermore show that these strains are compatible with automated genetic analysis. This study underscores the importance of analyzing mutant phenotypes and provides a resource to complement the yeast knockout collection. ..
  17. Gahura O, Abrhámová K, Skruzny M, Valentová A, Munzarová V, Folk P, et al. Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates. J Cell Biochem. 2009;106:139-51 pubmed publisher
    ..interacts with alleles of NTC members SYF1, CLF1/SYF3, NTC20, and CEF1, and 2nd step splicing factors SLU7, PRP17, PRP18, and PRP22...
  18. Kaplan Y, Kupiec M. A role for the yeast cell cycle/splicing factor Cdc40 in the G1/S transition. Curr Genet. 2007;51:123-40 pubmed
    ..A role of Cdc40p in passage through G(1)/S (START) is further supported by the enhanced temperature sensitivity and G(1)/S ..
  19. Seshadri V, Vaidya V, Vijayraghavan U. Genetic studies of the PRP17 gene of Saccharomyces cerevisiae: a domain essential for function maps to a nonconserved region of the protein. Genetics. 1996;143:45-55 pubmed
    The PRP17 gene product is required for the second step of pre-mRNA splicing reactions. The C-terminal half of this protein bears four repeat units with homology to the beta transducin repeat...