Gene Symbol: CDC31
Description: centrin
Alias: DSK1, centrin
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Spang A, Courtney I, Grein K, Matzner M, Schiebel E. The Cdc31p-binding protein Kar1p is a component of the half bridge of the yeast spindle pole body. J Cell Biol. 1995;128:863-77 pubmed
    ..V., S. L. Dyos, D. Kershaw, and J. T. Finch. 1993. J. Cell Biol. 123:1175-1184). The association of Kar1p with the spindle pole body was independent of Cdc31p. Cdc31p, on the other hand, was not associated with SPBs of kar1 cells. ..
  2. Jani D, Lutz S, Marshall N, Fischer T, Köhler A, Ellisdon A, et al. Sus1, Cdc31, and the Sac3 CID region form a conserved interaction platform that promotes nuclear pore association and mRNA export. Mol Cell. 2009;33:727-37 pubmed publisher
    The yeast Sac3:Cdc31:Sus1:Thp1 (TREX-2) complex facilitates the repositioning and association of actively transcribing genes with nuclear pores (NPCs)-"gene gating"-that is central to integrating transcription, processing, and ..
  3. Köhler A, Pascual García P, Llopis A, Zapater M, Posas F, Hurt E, et al. The mRNA export factor Sus1 is involved in Spt/Ada/Gcn5 acetyltransferase-mediated H2B deubiquitinylation through its interaction with Ubp8 and Sgf11. Mol Biol Cell. 2006;17:4228-36 pubmed
    Sus1 acts in nuclear mRNA export via its association with the nuclear pore-associated Sac3-Thp1-Cdc31 complex. In addition, Sus1 plays a role in transcription through its interaction with the Spt/Ada/Gcn5 acetyltransferase (SAGA) complex...
  4. Geier B, Wiech H, Schiebel E. Binding of centrins and yeast calmodulin to synthetic peptides corresponding to binding sites in the spindle pole body components Kar1p and Spc110p. J Biol Chem. 1996;271:28366-74 pubmed
    ..Interestingly, centrin binding was regulated by physiological relevant changes in [Ca2+], and this Ca2+ dependence was influenced by ..
  5. Vallen E, Ho W, Winey M, Rose M. Genetic interactions between CDC31 and KAR1, two genes required for duplication of the microtubule organizing center in Saccharomyces cerevisiae. Genetics. 1994;137:407-22 pubmed required for SPB duplication and encodes a calmodulin-like protein that is most closely related to caltractin/centrin, a protein associated with the Chlamydomonas basal body...
  6. Araki Y, Lau C, Maekawa H, Jaspersen S, Giddings T, Schiebel E, et al. The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p. Mol Biol Cell. 2006;17:1959-70 pubmed
    ..These results demonstrate that Nbp1p is a SPB component that acts in SPB duplication at the point of SPB insertion into the nuclear envelope. ..
  7. Fischer T, Rodriguez Navarro S, Pereira G, Rácz A, Schiebel E, Hurt E. Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. Nat Cell Biol. 2004;6:840-8 pubmed
    ..Here we describe a new function of the yeast centrin Cdc31...
  8. Ivanovska I, Rose M. Fine structure analysis of the yeast centrin, Cdc31p, identifies residues specific for cell morphology and spindle pole body duplication. Genetics. 2001;157:503-18 pubmed
    b>Centrin/Cdc31p is a Ca2+-binding protein related to calmodulin found in the MTOC of diverse organisms. In yeast, Cdc31p localizes to the SPB where it interacts with Kar1p and is required for SPB duplication...
  9. Elliott S, Knop M, Schlenstedt G, Schiebel E. Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication. Proc Natl Acad Sci U S A. 1999;96:6205-10 pubmed
    ..function of SPC29 during SPB assembly is indicated by the SPB duplication defect of conditional lethal spc29(ts) cells and by the genetic interaction of SPC29 with CDC31 and KAR1, two genes that are involved in SPB duplication.

More Information


  1. Kilmartin J. Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication. J Cell Biol. 2003;162:1211-21 pubmed
    Centrins are calmodulin-like proteins present in microtubule-organizing centers. The Saccharomyces cerevisiae centrin, Cdc31p, was functionally tagged with a single Z domain of protein A, and used in pull-down experiments to isolate ..
  2. Biggins S, Rose M. Direct interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body. J Cell Biol. 1994;125:843-52 pubmed
    ..The Cdc31 protein is most related to caltractin/centrin, a calcium-binding protein present in microtubule organizing centers in many organisms...
  3. Köhler A, Schneider M, Cabal G, Nehrbass U, Hurt E. Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export. Nat Cell Biol. 2008;10:707-15 pubmed publisher
    ..recruitment of the TREX-2 mRNA export factors Sac3 and Thp1 to SAGA and their stable interaction with Sus1-Cdc31. This latter step is crucial to target TREX-2 to the NPC...
  4. Sullivan D, Biggins S, Rose M. The yeast centrin, cdc31p, and the interacting protein kinase, Kic1p, are required for cell integrity. J Cell Biol. 1998;143:751-65 pubmed
    Cdc31p is the yeast homologue of centrin, a highly conserved calcium-binding protein of the calmodulin superfamily. Previously centrins have been implicated only in microtubule-based processes...
  5. Ellisdon A, Dimitrova L, Hurt E, Stewart M. Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex. Nat Struct Mol Biol. 2012;19:328-36 pubmed publisher
  6. Jaspersen S, Giddings T, Winey M. Mps3p is a novel component of the yeast spindle pole body that interacts with the yeast centrin homologue Cdc31p. J Cell Biol. 2002;159:945-56 pubmed
    ..vitro, as well as the fact that Cdc31p localization to the SPB is partially dependent on Mps3p function, suggest that one function for Mps3p during SPB duplication is to recruit Cdc31p, the yeast centrin homologue, to the half-bridge.
  7. Spang A, Courtney I, Fackler U, Matzner M, Schiebel E. The calcium-binding protein cell division cycle 31 of Saccharomyces cerevisiae is a component of the half bridge of the spindle pole body. J Cell Biol. 1993;123:405-16 pubmed
    b>Cdc31 mutants of Saccharomyces cerevisiae arrest at the nonpermissive temperature with large buds, G2 DNA content and, a single, abnormally large spindle pole body (SPB) (Byers, B. 1981. Molecular Genetics in Yeast...
  8. González Aguilera C, Tous C, Gómez González B, Huertas P, Luna R, Aguilera A. The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability. Mol Biol Cell. 2008;19:4310-8 pubmed publisher
    ..transcription and genome instability are consistent with the possibility that R-loops are formed in Thp1-Sac3-Sus1-Cdc31 as in THO mutants...
  9. Li S, Sandercock A, Conduit P, Robinson C, Williams R, Kilmartin J. Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication. J Cell Biol. 2006;173:867-77 pubmed
    ..The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new ..
  10. De M, Oleskie A, Ayyash M, Dutta S, Mancour L, Abazeed M, et al. The Vps13p-Cdc31p complex is directly required for TGN late endosome transport and TGN homotypic fusion. J Cell Biol. 2017;216:425-439 pubmed publisher
  11. Miron S, Durand D, Chilom C, Perez J, Craescu C. Binding of calcium, magnesium, and target peptides to Cdc31, the centrin of yeast Saccharomyces cerevisiae. Biochemistry. 2011;50:6409-22 pubmed publisher
    Cdc31, the Saccharomyces cerevisiae centrin, is an EF-hand calcium-binding protein essential for the cell division and mRNA nuclear export...
  12. Niepel M, Strambio de Castillia C, Fasolo J, Chait B, Rout M. The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly. J Cell Biol. 2005;170:225-35 pubmed
    ..Based on these data, we propose that Mlp2p links the SPB to the peripheral Mlp assembly, and that this linkage is required for efficient incorporation of components into the SPB. ..
  13. Klöckner C, Schneider M, Lutz S, Jani D, Kressler D, Stewart M, et al. Mutational uncoupling of the role of Sus1 in nuclear pore complex targeting of an mRNA export complex and histone H2B deubiquitination. J Biol Chem. 2009;284:12049-56 pubmed publisher
    ..Spt-Ada-Gcn5 acetyltransferase) transcriptional co-activator and the mRNA export complex, TREX-2 (Sus1-Sac3-Thp1-Cdc31)...
  14. Li P, Shao Y, Jin H, Yu H. Ndj1, a telomere-associated protein, regulates centrosome separation in budding yeast meiosis. J Cell Biol. 2015;209:247-59 pubmed publisher
    ..These findings reveal the underlying mechanism that coordinates yeast centrosome dynamics with meiotic telomere movement and cell cycle progression. ..
  15. Khalfan W, Ivanovska I, Rose M. Functional interaction between the PKC1 pathway and CDC31 network of SPB duplication genes. Genetics. 2000;155:1543-59 pubmed
    ..We also found that mutations in SPC110, which encodes a major SPB component, showed genetic interactions with both CDC31 and the PKC1 pathway...
  16. Chen L, Madura K. Centrin/Cdc31 is a novel regulator of protein degradation. Mol Cell Biol. 2008;28:1829-40 pubmed
    ..XPC/hHR23 was also reported to bind centrin-2, a member of the superfamily of calcium-binding EF-hand proteins...
  17. Weiss E, Winey M. The Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint. J Cell Biol. 1996;132:111-23 pubmed
    ..1991; Lauze et al., 1995), is also required for M-phase check-point function. In cdc31-2 and mps2-1 mutants, conditional failure of SPB duplication results in cell cycle arrest with high p34CDC28 ..
  18. Araki Y, Gombos L, Migueleti S, Sivashanmugam L, Antony C, Schiebel E. N-terminal regions of Mps1 kinase determine functional bifurcation. J Cell Biol. 2010;189:41-56 pubmed publisher SPB duplication and biochemical enrichment of Mps1 identified the essential SPB components Spc29 and the yeast centrin Cdc31 as Mps1 targets in SPB duplication...
  19. Keck J, Jones M, Wong C, Binkley J, Chen D, Jaspersen S, et al. A cell cycle phosphoproteome of the yeast centrosome. Science. 2011;332:1557-61 pubmed publisher
    ..Our work establishes the extent and complexity of this prominent posttranslational modification in centrosome biology and provides specific examples of phosphorylation control in centrosome function. ..
  20. Aibara S, Bai X, Stewart M. The Sac3 TPR-like region in the Saccharomyces cerevisiae TREX-2 complex is more extensive but independent of the CID region. J Struct Biol. 2016;195:316-324 pubmed publisher
    ..The TREX-2 complex is based on a Sac3 scaffold to which Thp1, Sem1, Cdc31, and Sus1 bind...
  21. Winsor B, Schiebel E. Review: an overview of the Saccharomyces cerevisiae microtubule and microfilament cytoskeleton. Yeast. 1997;13:399-434 pubmed
  22. Vasu S, Forbes D. Nuclear pores and nuclear assembly. Curr Opin Cell Biol. 2001;13:363-75 pubmed
  23. Jani D, Valkov E, Stewart M. Structural basis for binding the TREX2 complex to nuclear pores, GAL1 localisation and mRNA export. Nucleic Acids Res. 2014;42:6686-97 pubmed publisher
    The conserved Sac3:Thp1:Sem1:Sus1:Cdc31 (TREX2) complex binds to nuclear pore complexes (NPCs) and, in addition to integrating mRNA nuclear export with preceding steps in the gene expression pathway, facilitates re-positioning of highly ..
  24. Seybold C, Elserafy M, Rüthnick D, Ozboyaci M, Neuner A, Flottmann B, et al. Kar1 binding to Sfi1 C-terminal regions anchors the SPB bridge to the nuclear envelope. J Cell Biol. 2015;209:843-61 pubmed publisher
    ..Its cytoplasmic components are the membrane-anchored Kar1, the yeast centrin Cdc31, and the Cdc31-binding protein Sfi1...