Gene Symbol: CDC28
Description: cyclin-dependent serine/threonine-protein kinase CDC28
Alias: CDK1, HSL5, SRM5, cyclin-dependent serine/threonine-protein kinase CDC28
Species: Saccharomyces cerevisiae S288c
Products:     CDC28

Top Publications

  1. Jaspersen S, Charles J, Morgan D. Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14. Curr Biol. 1999;9:227-36 pubmed vivo at multiple CDK consensus sites during cell cycle stages when activity of the cyclin-dependent kinase Cdc28 is high and APC activity is low...
  2. de Bruin R, McDonald W, Kalashnikova T, Yates J, Wittenberg C. Cln3 activates G1-specific transcription via phosphorylation of the SBF bound repressor Whi5. Cell. 2004;117:887-98 pubmed
    ..Like mammalian Rb, Whi5 is a G1-specific transcriptional repressor antagonized by CDK. ..
  3. Eissler C, Mazon G, Powers B, Savinov S, Symington L, Hall M. The Cdk/cDc14 module controls activation of the Yen1 holliday junction resolvase to promote genome stability. Mol Cell. 2014;54:80-93 pubmed publisher
    ..The precise timing of Yen1 activation, governed by core cell-cycle regulators, helps coordinate DNA repair with chromosome segregation and safeguards against genome destabilization. ..
  4. Costanzo M, Nishikawa J, Tang X, Millman J, Schub O, Breitkreuz K, et al. CDK activity antagonizes Whi5, an inhibitor of G1/S transcription in yeast. Cell. 2004;117:899-913 pubmed
    ..Elimination of CDK activity at the end of mitosis allows Whi5 to reenter the nucleus to again repress G1/S transcription. These findings harmonize G1/S control in eukaryotes. ..
  5. Bouquin N, Barral Y, Courbeyrette R, Blondel M, Snyder M, Mann C. Regulation of cytokinesis by the Elm1 protein kinase in Saccharomyces cerevisiae. J Cell Sci. 2000;113 ( Pt 8):1435-45 pubmed
    A Saccharomyces cerevisiae mutant unable to grow in a cdc28-1N background was isolated and shown to be affected in the ELM1 gene. Elm1 is a protein kinase, thought to be a negative regulator of pseudo-hyphal growth...
  6. Henchoz S, Chi Y, Catarin B, Herskowitz I, Deshaies R, Peter M. Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast. Genes Dev. 1997;11:3046-60 pubmed
    ..Our results show that Far1p is regulated by ubiquitin-mediated proteolysis and suggest that phosphorylation of Far1p by the Cdc28p-Clnp kinase is part of the recognition signal for ubiquitination. ..
  7. Feldman R, Correll C, Kaplan K, Deshaies R. A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell. 1997;91:221-30 pubmed
    ..Taken together, these data illuminate the molecular basis for the G1/S transition in budding yeast and suggest a general mechanism for phosphorylation-targeted ubiquitination in eukaryotes. ..
  8. Lanker S, Valdivieso M, Wittenberg C. Rapid degradation of the G1 cyclin Cln2 induced by CDK-dependent phosphorylation. Science. 1996;271:1597-601 pubmed
    ..Cln2 phosphorylation is dependent on the Cdc28 protein kinase, the CDK that it activates...
  9. Vodenicharov M, Wellinger R. DNA degradation at unprotected telomeres in yeast is regulated by the CDK1 (Cdc28/Clb) cell-cycle kinase. Mol Cell. 2006;24:127-37 pubmed
    ..The data also show that completion of S phase and the activity of the S-Cdk1 kinase were required for telomere degradation...

More Information


  1. Loog M, Morgan D. Cyclin specificity in the phosphorylation of cyclin-dependent kinase substrates. Nature. 2005;434:104-8 pubmed
    ..budding yeast cyclins, the S-phase cyclin Clb5 and the M-phase cyclin Clb2, in the phosphorylation of 150 Cdk1 (Cdc28) substrates. About 24% of these proteins were phosphorylated more efficiently by Clb5-Cdk1 than Clb2-Cdk1...
  2. Ahn S, Tobe B, Fitz Gerald J, Anderson S, Acurio A, Kron S. Enhanced cell polarity in mutants of the budding yeast cyclin-dependent kinase Cdc28p. Mol Biol Cell. 2001;12:3589-600 pubmed
    ..independent pathways may underlie their common phenotype, because the mutations are scattered throughout the CDC28 sequence, complement each other, and confer diverse biochemical properties...
  3. Lusk C, Waller D, Makhnevych T, Dienemann A, Whiteway M, Thomas D, et al. Nup53p is a target of two mitotic kinases, Cdk1p and Hrr25p. Traffic. 2007;8:647-60 pubmed
    ..The ability of nup53 alleles containing Cdk1p site mutations to complement synthetic defects of nup53 Delta nup170 Delta strains is linked to a function for Nup53p in the spindle assembly checkpoint. ..
  4. Rudner A, Murray A. Phosphorylation by Cdc28 activates the Cdc20-dependent activity of the anaphase-promoting complex. J Cell Biol. 2000;149:1377-90 pubmed
    ..The mitotic activity of Cdc28 (Cdk1) is required to activate this form of the APC, and mutants that are impaired in mitotic Cdc28 function have ..
  5. Bailly E, Cabantous S, Sondaz D, Bernadac A, Simon M. Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck. J Cell Sci. 2003;116:4119-30 pubmed
    ..Taken together, our data uncover a new role for Bud3 in cytokinesis that correlates with its capacity to target Clb2 at the neck, independently of its well established cell-type-specific function in bud site selection. ..
  6. Juanes M, Khoueiry R, Kupka T, Castro A, Mudrak I, Ogris E, et al. Budding yeast greatwall and endosulfines control activity and spatial regulation of PP2A(Cdc55) for timely mitotic progression. PLoS Genet. 2013;9:e1003575 pubmed publisher
    Entry into mitosis is triggered by cyclinB/Cdk1, whose activity is abruptly raised by a positive feedback loop...
  7. Ghiara J, Richardson H, Sugimoto K, Henze M, Lew D, Wittenberg C, et al. A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis. Cell. 1991;65:163-74 pubmed
    ..This arrest can be reversed by inactivation of the Cdc28 protein kinase, suggesting that cyclin-mediated arrest results from persistent protein kinase activation.
  8. Goranov A, Cook M, Ricicova M, Ben Ari G, Gonzalez C, Hansen C, et al. The rate of cell growth is governed by cell cycle stage. Genes Dev. 2009;23:1408-22 pubmed publisher
    ..Our study uncovers a fundamental relationship whereby cell cycle position regulates growth. ..
  9. Segal M, Clarke D, Maddox P, Salmon E, Bloom K, Reed S. Coordinated spindle assembly and orientation requires Clb5p-dependent kinase in budding yeast. J Cell Biol. 2000;148:441-52 pubmed
    ..cerevisiae, loss of the S-phase B-type cyclin Clb5p under conditions of limited cyclin-dependent kinase activity (cdc28-4 clb5Delta cells) causes a spindle positioning defect that results in an undivided nucleus entering the bud...
  10. Maekawa H, Usui T, Knop M, Schiebel E. Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions. EMBO J. 2003;22:438-49 pubmed
    ..First, Kar9 binds to spindle pole bodies (SPBs) in G(1) of the cell cycle. Secondly, in G(1)/S the yeast Cdk1, Cdc28, associates with SPBs and phosphorylates Kar9...
  11. Benjamin K, Zhang C, Shokat K, Herskowitz I. Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2. Genes Dev. 2003;17:1524-39 pubmed
    Meiosis is thought to require the protein kinase Ime2 early for DNA replication and the cyclin-dependent kinase Cdc28 late for chromosome segregation...
  12. Li S, Makovets S, Matsuguchi T, Blethrow J, Shokat K, Blackburn E. Cdk1-dependent phosphorylation of Cdc13 coordinates telomere elongation during cell-cycle progression. Cell. 2009;136:50-61 pubmed publisher
    ..In S. cerevisiae, a single cyclin-dependent kinase Cdk1 (Cdc28) coordinates the serial events required for the cell division cycle, but no Cdk1 substrate has been identified ..
  13. Surana U, Robitsch H, Price C, Schuster T, Fitch I, Futcher A, et al. The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae. Cell. 1991;65:145-61 pubmed
    b>cdc28-1N is a conditional allele that has normal G1 (Start) function but confers a mitotic defect. We have isolated seven genes that in high dosage suppress the growth defect of cdc28-1N cells but not of Start-defective cdc28-4 cells...
  14. Skowyra D, Craig K, Tyers M, Elledge S, Harper J. F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell. 1997;91:209-19 pubmed
    ..SCF complexes couple protein kinase signaling pathways to the control of protein abundance. ..
  15. Yu V, Baskerville C, Grünenfelder B, Reed S. A kinase-independent function of Cks1 and Cdk1 in regulation of transcription. Mol Cell. 2005;17:145-51 pubmed
    ..a function in transcription for the Saccharomyces cerevisiae cell cycle regulatory cyclin-dependent kinase Cdc28 (Cdk1) and its interacting protein, Cks1...
  16. Wang Y, Burke D. Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiae. Mol Cell Biol. 1997;17:620-6 pubmed
    ..Finally, cdc55::LEU2 suppresses the temperature sensitivity of cdc20-1, suggesting additional roles for CDC55 in mitosis. ..
  17. Geymonat M, Spanos A, Wells G, Smerdon S, Sedgwick S. Clb6/Cdc28 and Cdc14 regulate phosphorylation status and cellular localization of Swi6. Mol Cell Biol. 2004;24:2277-85 pubmed
    ..We show that Clb6/Cdc28 kinase is required for this nuclear export...
  18. Trovesi C, Falcettoni M, Lucchini G, Clerici M, Longhese M. Distinct Cdk1 requirements during single-strand annealing, noncrossover, and crossover recombination. PLoS Genet. 2011;7:e1002263 pubmed publisher
    ..This cell cycle specificity depends on cyclin-dependent protein kinases (Cdk1 in Saccharomyces cerevisiae), which initiate HR by promoting 5'-3' nucleolytic degradation of the DSB ends...
  19. Rossio V, Galati E, Ferrari M, Pellicioli A, Sutani T, Shirahige K, et al. The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase. J Cell Biol. 2010;191:981-97 pubmed publisher
    ..Our data suggest that fine-tuning regulators of mitotic exit have important functions during mitotic progression in cells treated with microtubule poisons and might be promising targets for cancer treatment. ..
  20. Harvey S, Charlet A, Haas W, Gygi S, Kellogg D. Cdk1-dependent regulation of the mitotic inhibitor Wee1. Cell. 2005;122:407-20 pubmed
    The Wee1 kinase phosphorylates and inhibits cyclin-dependent kinase 1 (Cdk1), thereby delaying entry into mitosis until appropriate conditions have been met...
  21. Siegmund R, Nasmyth K. The Saccharomyces cerevisiae Start-specific transcription factor Swi4 interacts through the ankyrin repeats with the mitotic Clb2/Cdc28 kinase and through its conserved carboxy terminus with Swi6. Mol Cell Biol. 1996;16:2647-55 pubmed
    ..These events require activation of Cdc28 kinase by G1 cyclins...
  22. Peter M, Gartner A, Horecka J, Ammerer G, Herskowitz I. FAR1 links the signal transduction pathway to the cell cycle machinery in yeast. Cell. 1993;73:747-60 pubmed
    ..Here we show that FAR1 is a substrate for FUS3 and that this phosphorylation regulates association of FAR1 with CDC28-CLN2 kinase...
  23. McCusker D, Royou A, Velours C, Kellogg D. Cdk1-dependent control of membrane-trafficking dynamics. Mol Biol Cell. 2012;23:3336-47 pubmed publisher
    Cyclin-dependent kinase 1 (Cdk1) is required for initiation and maintenance of polarized cell growth in budding yeast...
  24. Gartner A, Jovanovic A, Jeoung D, Bourlat S, Cross F, Ammerer G. Pheromone-dependent G1 cell cycle arrest requires Far1 phosphorylation, but may not involve inhibition of Cdc28-Cln2 kinase, in vivo. Mol Cell Biol. 1998;18:3681-91 pubmed
    ..wild-type Far1 protein, not the T306-to-A substitution product, can be found in stable association with the Cdc28-Cln2 complex...
  25. Okuzaki D, Watanabe T, Tanaka S, Nojima H. The Saccharomyces cerevisiae bud-neck proteins Kcc4 and Gin4 have distinct but partially-overlapping cellular functions. Genes Genet Syst. 2003;78:113-26 pubmed
    ..cerevisiae, Swe1 delays the onset of mitosis by phosphorylation and inactivation of the cyclin-dependent kinase Cdc28, thereby relaying the morphogenetic signal to the cell cycle...
  26. Knaus M, Pelli Gulli M, van Drogen F, Springer S, Jaquenoud M, Peter M. Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence. EMBO J. 2007;26:4501-13 pubmed
  27. Moll T, Tebb G, Surana U, Robitsch H, Nasmyth K. The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5. Cell. 1991;66:743-58 pubmed
    ..As all three serines are phosphorylated by purified CDC28-dependent H1 kinase activity in vitro, we propose a model in which the CDC28 kinase acts directly to control ..
  28. Holt L, Hutti J, Cantley L, Morgan D. Evolution of Ime2 phosphorylation sites on Cdk1 substrates provides a mechanism to limit the effects of the phosphatase Cdc14 in meiosis. Mol Cell. 2007;25:689-702 pubmed
    Progression through meiosis in yeast is governed by the cyclin-dependent kinase Cdk1, in concert with a related kinase called Ime2. It remains unclear how these kinases collaborate to meet the unique demands of meiotic progression...
  29. Kito K, Kawaguchi N, Okada S, Ito T. Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics. Proteomics. 2008;8:2366-70 pubmed publisher
    ..We successfully applied this strategy to analyze two yeast protein complexes, eIF2B-eIF2 and cyclin-Cdc28.
  30. Adrover M, Zi Z, Duch A, Schaber J, Gonzalez Novo A, Jimenez J, et al. Time-dependent quantitative multicomponent control of the G?-S network by the stress-activated protein kinase Hog1 upon osmostress. Sci Signal. 2011;4:ra63 pubmed publisher
    ..phosphorylation and degradation of Sic1 at Start prevented residual activity of the cyclin/CDK complex Clb5/Cdc28 from initiating DNA replication before adaptation to the stress...
  31. Hadwiger J, Wittenberg C, Mendenhall M, Reed S. The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex. Mol Cell Biol. 1989;9:2034-41 pubmed
    The Saccharomyces cerevisiae gene CDC28 encodes a protein kinase required for cell cycle initiation...
  32. Kono K, Nogami S, Abe M, Nishizawa M, Morishita S, Pellman D, et al. G1/S cyclin-dependent kinase regulates small GTPase Rho1p through phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae. Mol Biol Cell. 2008;19:1763-71 pubmed publisher
    ..We propose that Cln2p/Cdc28p-dependent phosphorylation of Tus1p is required for appropriate temporal and spatial activation of Rho1p at the G1/S transition. ..
  33. Cheng A, Ross K, Kaldis P, Solomon M. Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases. Genes Dev. 1999;13:2946-57 pubmed
    ..Furthermore, PP2C-like enzymes are the predominant phosphatases toward human Cdk2 in HeLa cell extracts, indicating that the substrate specificity of PP2Cs toward CDKs is evolutionarily conserved. ..
  34. Wijnen H, Futcher B. Genetic analysis of the shared role of CLN3 and BCK2 at the G(1)-S transition in Saccharomyces cerevisiae. Genetics. 1999;153:1131-43 pubmed
    ..Unlike Cln3, Bck2 is capable of inducing its transcriptional targets in the absence of functional Cdc28. Our results revealed promoter-specific mechanisms of regulation by Cln3, Bck2, SBF, and MBF...
  35. Liang H, Lim H, Venkitaraman A, Surana U. Cdk1 promotes kinetochore bi-orientation and regulates Cdc20 expression during recovery from spindle checkpoint arrest. EMBO J. 2012;31:403-16 pubmed publisher
    ..Here, we report two unrecognized functions of yeast Cdk1 required for efficient recovery from SAC-induced arrest...
  36. Tyers M, Tokiwa G, Futcher B. Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins. EMBO J. 1993;12:1955-68 pubmed
    ..cerevisiae, the G1 cyclins Cln1, Cln2 and Cln3 regulate entry into the cell cycle (Start) by activating the Cdc28 protein kinase...
  37. Ira G, Pellicioli A, Balijja A, Wang X, Fiorani S, Carotenuto W, et al. DNA end resection, homologous recombination and DNA damage checkpoint activation require CDK1. Nature. 2004;431:1011-7 pubmed
    ..Here we report that DNA damage checkpoint activation by a DSB requires the cyclin-dependent kinase CDK1 (Cdc28) in budding yeast. CDK1 is also required for DSB-induced homologous recombination at any cell cycle stage...
  38. Lyons N, Morgan D. Cdk1-dependent destruction of Eco1 prevents cohesion establishment after S phase. Mol Cell. 2011;42:378-89 pubmed publisher
    ..Here, we show that Eco1 is negatively regulated by the protein kinase Cdk1. Phosphorylation of Eco1 after S phase targets it to SCF(Cdc4) for ubiquitination and subsequent degradation...
  39. Keck J, Jones M, Wong C, Binkley J, Chen D, Jaspersen S, et al. A cell cycle phosphoproteome of the yeast centrosome. Science. 2011;332:1557-61 pubmed publisher
    ..Our work establishes the extent and complexity of this prominent posttranslational modification in centrosome biology and provides specific examples of phosphorylation control in centrosome function. ..
  40. Costanzo M, Schub O, Andrews B. G1 transcription factors are differentially regulated in Saccharomyces cerevisiae by the Swi6-binding protein Stb1. Mol Cell Biol. 2003;23:5064-77 pubmed
    ..Chromatin immunoprecipitation experiments confirm that Stb1 localizes to promoters of MBF-regulated genes. Our data indicate that, contrary to previous models, MBF and SBF have unique components and might be distinctly regulated. ..
  41. Verma R, Feldman R, Deshaies R. SIC1 is ubiquitinated in vitro by a pathway that requires CDC4, CDC34, and cyclin/CDK activities. Mol Biol Cell. 1997;8:1427-37 pubmed
    ..SIC1 proteolysis is mediated by the ubiquitin pathway and requires the action of CDC34, CDC4, CDC53, SKP1, and CLN/CDC28. As a first step in defining the functions of the corresponding gene products, we have reconstituted SIC1 ..
  42. McMillan J, Theesfeld C, Harrison J, Bardes E, Lew D. Determinants of Swe1p degradation in Saccharomyces cerevisiae. Mol Biol Cell. 2002;13:3560-75 pubmed
    ..The other region did not appear to affect interactions with known Swe1p regulators, suggesting that other as-yet-unknown regulators exist. ..
  43. Kaiser P, Moncollin V, Clarke D, Watson M, Bertolaet B, Reed S, et al. Cyclin-dependent kinase and Cks/Suc1 interact with the proteasome in yeast to control proteolysis of M-phase targets. Genes Dev. 1999;13:1190-202 pubmed
    ..components of the cell cycle machinery in yeast, specifically the cell cycle regulatory cyclin-dependent kinase Cdc28 and a conserved associated protein Cks1/Suc1, interact genetically, physically, and functionally with components ..
  44. Lin T, Neuner A, Schlosser Y, Scharf A, Weber L, Schiebel E. Cell-cycle dependent phosphorylation of yeast pericentrin regulates ?-TuSC-mediated microtubule nucleation. elife. 2014;3:e02208 pubmed publisher
    ..Here we show that Spc110 phosphorylated by Mps1 and Cdk1 activates ?-TuSC oligomerization and MT nucleation in a cell cycle dependent manner...
  45. Lim H, Goh P, Surana U. Spindle pole body separation in Saccharomyces cerevisiae requires dephosphorylation of the tyrosine 19 residue of Cdc28. Mol Cell Biol. 1996;16:6385-97 pubmed
    ..It is known that in the budding yeast Saccharomyces cerevisiae, a homologous kinase, Cdc28, mediates the progression through M phase, but it is not clear what specific mitotic function its activation by ..
  46. Edgington N, Blacketer M, Bierwagen T, Myers A. Control of Saccharomyces cerevisiae filamentous growth by cyclin-dependent kinase Cdc28. Mol Cell Biol. 1999;19:1369-80 pubmed
    ..A specific amino acid substitution in the cyclin-dependent protein kinase Cdc28 was found to cause constitutive expression of most filamentous growth characteristics...
  47. Hu F, Gan Y, Aparicio O. Identification of Clb2 residues required for Swe1 regulation of Clb2-Cdc28 in Saccharomyces cerevisiae. Genetics. 2008;179:863-74 pubmed publisher
    ..Saccharomyces Wee1 (Swe1) inhibits Cdc28 (Cdk1) associated with the mitotic cyclin, Clb2, but not with the G(1) (Cln1, -2, and -3) or the S-phase (Clb5 and ..
  48. Pal G, Paraz M, Kellogg D. Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1. J Cell Biol. 2008;180:931-45 pubmed publisher
    The Cdc25 phosphatase promotes entry into mitosis by removing cyclin-dependent kinase 1 (Cdk1) inhibitory phosphorylation...
  49. Agarwal R, COHEN FIX O. Phosphorylation of the mitotic regulator Pds1/securin by Cdc28 is required for efficient nuclear localization of Esp1/separase. Genes Dev. 2002;16:1371-82 pubmed
    ..Here we show that Pds1 is a substrate of the cyclin-dependent kinase Cdc28. Phosphorylation of Pds1 by Cdc28 is important for efficient binding of Pds1 to Esp1 and for promoting the nuclear ..
  50. Bremmer S, Hall H, Martinez J, Eissler C, Hinrichsen T, Rossie S, et al. Cdc14 phosphatases preferentially dephosphorylate a subset of cyclin-dependent kinase (Cdk) sites containing phosphoserine. J Biol Chem. 2012;287:1662-9 pubmed publisher
    ..The intrinsic selectivity of Cdc14 may help establish the order of Cdk substrate dephosphorylation during mitotic exit and contribute to roles in other cellular processes. ..
  51. Yu Y, Jiang Y, Wellinger R, Carlson K, Roberts J, Stillman D. Mutations in the homologous ZDS1 and ZDS2 genes affect cell cycle progression. Mol Cell Biol. 1996;16:5254-63 pubmed
    ..We show that at semipermissive temperatures, a yeast strain with a cdc28-1N allele was uniquely deficient in plasmid maintenance in comparison with strains harboring other cdc28 ..
  52. Segal M, Clarke D, Reed S. Clb5-associated kinase activity is required early in the spindle pathway for correct preanaphase nuclear positioning in Saccharomyces cerevisiae. J Cell Biol. 1998;143:135-45 pubmed
    In Saccharomyces cerevisiae, a single cyclin-dependent kinase, Cdc28, regulates both G1/S and G2/M phase transitions by associating with stage-specific cyclins...
  53. Heo S, Tatebayashi K, Ikeda H. The budding yeast cohesin gene SCC1/MCD1/RHC21 genetically interacts with PKA, CDK and APC. Curr Genet. 1999;36:329-38 pubmed
    ..Thirdly, ZDS1, a multicopy suppressor of cdc28-1N, and its homologue ZDS2 were isolated as multicopy suppressors of rhc21-sk16...
  54. Wicky S, Tjandra H, Schieltz D, Yates J, Kellogg D. The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphatase. Mol Biol Cell. 2011;22:20-32 pubmed publisher
    The Wee1 kinase restrains entry into mitosis by phosphorylating and inhibiting cyclin-dependent kinase 1 (Cdk1). The Cdc25 phosphatase promotes entry into mitosis by removing Cdk1 inhibitory phosphorylation...
  55. Kõivomägi M, Ord M, Iofik A, Valk E, Venta R, Faustova I, et al. Multisite phosphorylation networks as signal processors for Cdk1. Nat Struct Mol Biol. 2013;20:1415-24 pubmed publisher
    ..We show that cyclin-Cdk1-Cks1-dependent phosphorylation of multisite targets in Saccharomyces cerevisiae is controlled by key substrate ..
  56. Frank C, Hyde M, Greider C. Regulation of telomere elongation by the cyclin-dependent kinase CDK1. Mol Cell. 2006;24:423-32 pubmed
    ..We used an assay that detects de novo telomere addition to examine the role of the cyclin-dependent kinase Cdk1 (Cdc28) in cell-cycle-specific telomere elongation...
  57. Mimura S, Seki T, Tanaka S, Diffley J. Phosphorylation-dependent binding of mitotic cyclins to Cdc6 contributes to DNA replication control. Nature. 2004;431:1118-23 pubmed
    ..We show here that the mitotic CDK, Clb2/Cdc28, binds tightly to an amino-terminal domain (NTD) of Cdc6, and that Cdc6 in this complex is unable to assemble pre-..
  58. Akiyoshi B, Biggins S. Cdc14-dependent dephosphorylation of a kinetochore protein prior to anaphase in Saccharomyces cerevisiae. Genetics. 2010;186:1487-91 pubmed publisher
    The budding yeast Cdc14 phosphatase reverses Cdk1 phosphorylation to promote mitotic exit...
  59. Maekawa H, Schiebel E. Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex. Genes Dev. 2004;18:1709-24 pubmed
    ..It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, associates only with the SPB in the mother cell and so prevents Kar9 binding to this ..
  60. Lin F, Arndt K. The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis. EMBO J. 1995;14:2745-59 pubmed
    ..a shift to the non-permissive temperature, the pph21-102 cells were blocked in G2 and had low activity of Clb2-Cdc28 kinase. Expression of Clb2 from the S...
  61. Saponaro M, Callahan D, Zheng X, Krejci L, Haber J, Klein H, et al. Cdk1 targets Srs2 to complete synthesis-dependent strand annealing and to promote recombinational repair. PLoS Genet. 2010;6:e1000858 pubmed publisher
    b>Cdk1 kinase phosphorylates budding yeast Srs2, a member of UvrD protein family, displays both DNA translocation and DNA unwinding activities in vitro...
  62. Huisman S, Smeets M, Segal M. Phosphorylation of Spc110p by Cdc28p-Clb5p kinase contributes to correct spindle morphogenesis in S. cerevisiae. J Cell Sci. 2007;120:435-46 pubmed
    ..Together, our results point to the function of cyclin-dependent kinase phosphorylation of Spc110p and provide, in addition, support to a model for Clb5p control of spindle polarity at the level of astral microtubule organisation. ..
  63. Asano S, Park J, Sakchaisri K, Yu L, Song S, Supavilai P, et al. Concerted mechanism of Swe1/Wee1 regulation by multiple kinases in budding yeast. EMBO J. 2005;24:2194-204 pubmed
    ..Mitotic cyclin (Clb2)-bound Cdc28 (Cdk1 homolog) directly phosphorylated Swe1 and this modification served as a priming step to promote subsequent ..
  64. Lopez Mosqueda J, Maas N, Jonsson Z, Defazio Eli L, Wohlschlegel J, Toczyski D. Damage-induced phosphorylation of Sld3 is important to block late origin firing. Nature. 2010;467:479-83 pubmed publisher
    ..These data indicate that the intra-S-phase checkpoint functions to block late origin firing in adverse conditions to prevent genomic instability and maximize cell survival. ..
  65. Amon A, Tyers M, Futcher B, Nasmyth K. Mechanisms that help the yeast cell cycle clock tick: G2 cyclins transcriptionally activate G2 cyclins and repress G1 cyclins. Cell. 1993;74:993-1007 pubmed
    ..Furthermore, SW14 associates with CLB2 protein and is a substrate for the CLB2-associated CDC28 kinase in vitro.
  66. Cross F, Jacobson M. Conservation and function of a potential substrate-binding domain in the yeast Clb5 B-type cyclin. Mol Cell Biol. 2000;20:4782-90 pubmed
    ..These results support the idea of a modular, structurally conserved cyclin domain involved in substrate targeting. ..
  67. Rice L, Plakas C, Nickels J. Loss of meiotic rereplication block in Saccharomyces cerevisiae cells defective in Cdc28p regulation. Eukaryot Cell. 2005;4:55-62 pubmed
    ..the loss of function of the Schizosaccharomyces pombe wee1 ortholog Swe1p or through the expression of a dominant CDC28 allele, CDC28AF--diploid yeast cells are able to complete several rounds of premeiotic DNA replication within a ..
  68. Peng Y, Weisman L. The cyclin-dependent kinase Cdk1 directly regulates vacuole inheritance. Dev Cell. 2008;15:478-85 pubmed publisher
    ..Here we show that the yeast cyclin-dependent kinase Cdk1 phosphorylates Vac17 and that phosphorylation of Vac17 parallels cell cycle-dependent movement of the vacuole...
  69. Kitazono A, Garza D, Kron S. Mutations in the yeast cyclin-dependent kinase Cdc28 reveal a role in the spindle assembly checkpoint. Mol Genet Genomics. 2003;269:672-84 pubmed
    ..b>Cdc28 is the main CDK driving the cell cycle in budding yeast...
  70. Zachariae W, Schwab M, Nasmyth K, Seufert W. Control of cyclin ubiquitination by CDK-regulated binding of Hct1 to the anaphase promoting complex. Science. 1998;282:1721-4 pubmed
    ..The mutual inhibition between APC and CDKs explains how cells suppress mitotic CDK activity during G1 and then establish a period with elevated kinase activity from S phase until anaphase. ..
  71. Tyers M, Tokiwa G, Nash R, Futcher B. The Cln3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation. EMBO J. 1992;11:1773-84 pubmed
    ..cerevisiae, several of the proteins involved in the Start decision have been identified; these include the Cdc28 protein kinase and three cyclin-like proteins, Cln1, Cln2 and Cln3...
  72. Ross K, Kaldis P, Solomon M. Activating phosphorylation of the Saccharomyces cerevisiae cyclin-dependent kinase, cdc28p, precedes cyclin binding. Mol Biol Cell. 2000;11:1597-609 pubmed
    ..We propose that Cdc28p is normally phosphorylated by Cak1p before it binds cyclin. This activation pathway contrasts with that in higher eukaryotes, in which cyclin binding appears to precede activating phosphorylation. ..
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    ..Taken together, our data indicate that Cln3 is a molecular target of the Pho85 kinase that is required to modulate cell cycle entry in response to environmental changes in nutrient availability. ..
  74. Peter M, Herskowitz I. Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1. Science. 1994;265:1228-31 pubmed
    ..mediated by activation of a signal transduction pathway that results in inhibition of the cyclin-dependent kinase Cdc28-Cln. The Far1 protein is required for cell cycle arrest and associates with the Cdc28-Cln complex...
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    ..Cln2 and Cdc28 subunits coexpressed in baculovirus-infected insect cells fail to exhibit protein kinase activity towards multiple ..
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    ..Here we show that Clb-CDK1 phosphorylates Rad53 at Ser(774) in metaphase...
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    ..Ace2p is therefore controlled throughout the cell cycle at three levels: transcription, nuclear localisation, and proteolysis. ..
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    ..Activation of S-Cdk reported herein represents a complete reconstitution of the regulatory switch underlying the G1/S transition in budding yeast. ..