CDC26

Summary

Gene Symbol: CDC26
Description: anaphase promoting complex subunit CDC26
Alias: HIT3, SCD26, anaphase promoting complex subunit CDC26
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Zachariae W, Nasmyth K. Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev. 1999;13:2039-58 pubmed
  2. Hwang L, Murray A. A novel yeast screen for mitotic arrest mutants identifies DOC1, a new gene involved in cyclin proteolysis. Mol Biol Cell. 1997;8:1877-87 pubmed
    ..We show that mutations in CDC26 and a novel gene, DOC1, also prevent mitotic cyclin proteolysis...
  3. Passmore L, Booth C, Venien Bryan C, Ludtke S, Fioretto C, Johnson L, et al. Structural analysis of the anaphase-promoting complex reveals multiple active sites and insights into polyubiquitylation. Mol Cell. 2005;20:855-66 pubmed
    ..Our data suggest that multimerisation and/or the presence of multiple active sites facilitates the APC/C's ability to elongate polyubiquitin chains. ..
  4. Zachariae W, Shevchenko A, Andrews P, Ciosk R, Galova M, Stark M, et al. Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science. 1998;279:1216-9 pubmed
    ..Apc2p is similar to the cullin Cdc53p, which is a subunit of the ubiquitin-protein ligase complex SCFCdc4 required for the initiation of DNA replication. ..
  5. Zachariae W, Shin T, Galova M, Obermaier B, Nasmyth K. Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae. Science. 1996;274:1201-4 pubmed
    ..A small heat-inducible subunit is encoded by the CDC26 gene. The yeast APC is a 36S particle that contains at least seven different proteins.
  6. Passmore L, McCormack E, Au S, Paul A, Willison K, Harper J, et al. Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition. EMBO J. 2003;22:786-96 pubmed
    ..These results imply that Doc1p/Apc10 may play a role to regulate the binding of specific substrates, similar to that of the coactivators. ..
  7. Passmore L, Barford D, Harper J. Purification and assay of the budding yeast anaphase-promoting complex. Methods Enzymol. 2005;398:195-219 pubmed
    ..Activity assays toward several endogenous substrates, including Clb2 and Pds1, are described. In addition, methods for observation of APC-coactivator and APC-substrate complexes by native gel electrophoresis are described. ..
  8. Harkness T, Davies G, Ramaswamy V, Arnason T. The ubiquitin-dependent targeting pathway in Saccharomyces cerevisiae plays a critical role in multiple chromatin assembly regulatory steps. Genetics. 2002;162:615-32 pubmed
    ..The finding that the distinctly different E3 enzymes, APC and Rsp5p, both play roles in regulating chromatin assembly highlight the depth of the regulatory networks at play. The significance of these findings will be discussed. ..
  9. Hall M, Torres M, Schroeder G, Borchers C. Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex. J Biol Chem. 2003;278:16698-705 pubmed
    ..Taken together, these results demonstrate that Swm1 and Mnd2 are functional components of the yeast APC. ..

More Information

Publications23

  1. Schwickart M, Havlis J, Habermann B, Bogdanova A, Camasses A, Oelschlaegel T, et al. Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27. Mol Cell Biol. 2004;24:3562-76 pubmed
    ..Accordingly, Swm1/Apc13 is required for ubiquitin ligase activity in vitro and for the timely execution of APC/C-dependent cell cycle events in vivo. ..
  2. Wang J, Dye B, Rajashankar K, Kurinov I, Schulman B. Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC6 structure. Nat Struct Mol Biol. 2009;16:987-9 pubmed publisher
    The multisubunit anaphase promoting complex (APC) is an essential cell-cycle regulator. Although CDC26 is known to have a role in APC assembly, its molecular function has remained unclear...
  3. Hein J, Nilsson J. Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint. EMBO Rep. 2014;15:264-72 pubmed publisher
    ..We thus show that stable MCC association with the APC/C is critical for a functional SAC. ..
  4. Makhnevych T, Wong P, Pogoutse O, Vizeacoumar F, Greenblatt J, Emili A, et al. Hsp110 is required for spindle length control. J Cell Biol. 2012;198:623-36 pubmed publisher
    ..These data suggest a model whereby the Hsp70-Hsp110 chaperone complex antagonizes Cin8 plus-end motility and prevents premature spindle elongation in S phase. ..
  5. Ko N, Nishihama R, Tully G, Ostapenko D, Solomon M, Morgan D, et al. Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis. Mol Biol Cell. 2007;18:5139-53 pubmed
    ..Together, the data suggest that compromise of APC/C function allows the accumulation of Iqg1p, which then promotes actomyosin-ring-independent cytokinesis at least in part by activation of Cyk3p. ..
  6. Blethrow J, Tang C, Deng C, Krutchinsky A. Modular mass spectrometric tool for analysis of composition and phosphorylation of protein complexes. PLoS ONE. 2007;2:e358 pubmed
  7. Page A, Aneliunas V, Lamb J, Hieter P. In vivo characterization of the nonessential budding yeast anaphase-promoting complex/cyclosome components Swm1p, Mnd2p and Apc9p. Genetics. 2005;170:1045-62 pubmed
    ..Furthermore, the role of Apc9p in APC/C-dependent ubiquitination may be limited to the proteolysis of a select number of substrates. ..
  8. Oelschlaegel T, Schwickart M, Matos J, Bogdanova A, Camasses A, Havlis J, et al. The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. Cell. 2005;120:773-88 pubmed
    ..In vitro, Mnd2 inhibits ubiquitination of Pds1 by APC/C-Ama1 but not by other APC/C holo-enzymes. We conclude that chromosome segregation in meiosis depends on the selective inhibition of a meiosis-specific form of the APC/C. ..
  9. Rudner A, Hardwick K, Murray A. Cdc28 activates exit from mitosis in budding yeast. J Cell Biol. 2000;149:1361-76 pubmed
    ..The defects of CDC28-VF suggest that Cdc28 activity is required to induce the metaphase to anaphase transition and initiate the transition from anaphase to G1 in budding yeast...
  10. Grossberger R, Gieffers C, Zachariae W, Podtelejnikov A, Schleiffer A, Nasmyth K, et al. Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex. J Biol Chem. 1999;274:14500-7 pubmed
    ..DOC domains may therefore be important for reactions catalyzed by several types of ubiquitin-protein ligases. ..
  11. Harkness T, Arnason T, Legrand C, Pisclevich M, Davies G, Turner E. Contribution of CAF-I to anaphase-promoting-complex-mediated mitotic chromatin assembly in Saccharomyces cerevisiae. Eukaryot Cell. 2005;4:673-84 pubmed
    ..However, Asf1p and an intact CAF-I complex are dispensable for CAF-I subunit suppression, whereas CAF-I is necessary for ASF1, HIR1, and HIR2 suppression of apc5(CA) phenotypes. We discuss the implications of our observations. ..
  12. Jessulat M, Alamgir M, Salsali H, Greenblatt J, Xu J, Golshani A. Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae. Arch Biochem Biophys. 2008;469:157-64 pubmed
    ..We propose that one function of the Rtt109-Vps75 interacting protein pair is to affect the efficiency of NHEJ in yeast. Vps75 but not Rtt109 also seem to have an effect on the efficiency of DSB repair using homologous recombination. ..
  13. da Fonseca P, Kong E, Zhang Z, Schreiber A, Williams M, Morris E, et al. Structures of APC/C(Cdh1) with substrates identify Cdh1 and Apc10 as the D-box co-receptor. Nature. 2011;470:274-8 pubmed publisher
    ..Our results rationalize the contribution of both co-activator and core APC/C subunits to D-box recognition and provide a structural framework for understanding mechanisms of substrate recognition and catalysis by the APC/C. ..
  14. Rossio V, Michimoto T, Sasaki T, Ohbayashi I, Kikuchi Y, Yoshida S. Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint. J Cell Sci. 2013;126:4396-405 pubmed publisher
    ..Because the Cdc55-binding proteins Zds1 and Zds2 inhibit both nuclear accumulation of Cdc55 and SAC activity, we propose that spatial control of PP2A by Zds1 family proteins is important for tight control of SAC and mitotic progression. ..