Gene Symbol: CDC25
Description: Ras family guanine nucleotide exchange factor CDC25
Alias: CTN1, Ras family guanine nucleotide exchange factor CDC25
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Easlon E, Tsang F, Dilova I, Wang C, Lu S, Skinner C, et al. The dihydrolipoamide acetyltransferase is a novel metabolic longevity factor and is required for calorie restriction-mediated life span extension. J Biol Chem. 2007;282:6161-71 pubmed
    ..CR may therefore also extend life span and ameliorate age-associated diseases by increasing metabolic fitness through regulating central metabolic enzymes. ..
  2. Lin S, Defossez P, Guarente L. Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae. Science. 2000;289:2126-8 pubmed
    ..These findings suggest that the increased longevity induced by calorie restriction requires the activation of Sir2p by NAD. ..
  3. Tsang F, James C, Kato M, Myers V, Ilyas I, Tsang M, et al. Reduced Ssy1-Ptr3-Ssy5 (SPS) signaling extends replicative life span by enhancing NAD+ homeostasis in Saccharomyces cerevisiae. J Biol Chem. 2015;290:12753-64 pubmed publisher
    ..Our findings may contribute to understanding the molecular basis of NAD(+) metabolism, cellular life span, and diseases associated with NAD(+) deficiency and aging. ..
  4. Gombault A, Warringer J, Caesar R, Godin F, Vallee B, Doudeau M, et al. A phenotypic study of TFS1 mutants differentially altered in the inhibition of Ira2p or CPY. FEMS Yeast Res. 2009;9:867-74 pubmed publisher
    ..The results obtained demonstrate that the inhibition of Ira2p by Tfs1p is the predominant function under the conditions tested. ..
  5. Deminoff S, Howard S, Hester A, Warner S, Herman P. Using substrate-binding variants of the cAMP-dependent protein kinase to identify novel targets and a kinase domain important for substrate interactions in Saccharomyces cerevisiae. Genetics. 2006;173:1909-17 pubmed
    ..Moreover, the high conservation of the residues altered and, in particular, the invariant nature of the R324 position suggest that this approach might be generally applicable to other protein kinases. ..
  6. Cherkasova V, McCully R, Wang Y, Hinnebusch A, Elion E. A novel functional link between MAP kinase cascades and the Ras/cAMP pathway that regulates survival. Curr Biol. 2003;13:1220-6 pubmed
    ..The conserved RasGEF Cdc25 is a likely control point, because Kss1 and Fus3 complexes associate with and phosphorylate Cdc25...
  7. Mintzer K, Field J. The SH3 domain of the S. cerevisiae Cdc25p binds adenylyl cyclase and facilitates Ras regulation of cAMP signalling. Cell Signal. 1999;11:127-35 pubmed
    b>Cdc25 and Ras are two proteins required for cAMP signalling in the budding yeast Saccharomyces cerevisiae. Cdc25 is the guanine nucleotide exchange protein that activates Ras. Ras, in turn, activates adenylyl cyclase...
  8. Geymonat M, Wang L, Garreau H, Jacquet M. Ssa1p chaperone interacts with the guanine nucleotide exchange factor of ras Cdc25p and controls the cAMP pathway in Saccharomyces cerevisiae. Mol Microbiol. 1998;30:855-64 pubmed
    ..The effect of ssa deletion on glycogen accumulation was lost in a strain deleted for CDC25 rescued by the RAS2ile152 allele...
  9. Ruggieri R, Bender A, Matsui Y, Powers S, Takai Y, Pringle J, et al. RSR1, a ras-like gene homologous to Krev-1 (smg21A/rap1A): role in the development of cell polarity and interactions with the Ras pathway in Saccharomyces cerevisiae. Mol Cell Biol. 1992;12:758-66 pubmed
    ..This interaction of Rsr1p with the effector of Ras in S. cerevisiae suggests that Krev-1 may revert ras-induced transformation of mammalian cells by affecting the interaction of ras p21 with its effector. ..

More Information


  1. Frascotti G, Coccetti P, Vanoni M, Alberghina L, Martegani E. The overexpression of the 3' terminal region of the CDC25 gene of Saccharomyces cerevisiae causes growth inhibition and alteration of purine nucleotides pools. Biochim Biophys Acta. 1991;1089:206-12 pubmed
    The CDC25 gene is transcribed at a very low level in S. cerevisiae cells...
  2. Irniger S, Baumer M, Braus G. Glucose and ras activity influence the ubiquitin ligases APC/C and SCF in Saccharomyces cerevisiae. Genetics. 2000;154:1509-21 pubmed
    ..defective in the APC/C are suppressed by reduced Ras signaling activity, by a deletion of the RAS2 gene, by a cdc25 mutation, by elevated levels of PDE2, or by growth without glucose...
  3. Belotti F, Tisi R, Paiardi C, Rigamonti M, Groppi S, Martegani E. Localization of Ras signaling complex in budding yeast. Biochim Biophys Acta. 2012;1823:1208-16 pubmed publisher
    ..cAMP is produced by adenylate cyclase, which is activated both by Gpr1/Gpa2 system and Ras proteins, regulated by Cdc25/Sdc25 guanine exchange factors and Ira GTPase activator proteins...
  4. Camus C, Geymonat M, Garreau H, Baudet Nessler S, Jacquet M. Dimerization of Cdc25p, the guanine-nucleotide exchange factor for Ras from Saccharomyces cerevisiae, and its interaction with Sdc25p. Eur J Biochem. 1997;247:703-8 pubmed
    ..The two-hybrid system showed that the C-terminal part of Cdc25p (Cdc25-Ct) can interact with itself but also with Sdc25p-Ct, the corresponding part of Sdc25p, the other guanine exchange ..
  5. Perlman R, Yablonski D, Simchen G, Levitzki A. Cloning of the STE5 gene of Saccharomyces cerevisiae as a suppressor of the mating defect of cdc25 temperature-sensitive mutants. Proc Natl Acad Sci U S A. 1993;90:5474-8 pubmed
    ..RNA blot analysis reveals that STE5 gene transcription is regulated by the mating type of the cell and depends on an intact pheromone-response pathway. ..
  6. Toda T, Cameron S, Sass P, Wigler M. SCH9, a gene of Saccharomyces cerevisiae that encodes a protein distinct from, but functionally and structurally related to, cAMP-dependent protein kinase catalytic subunits. Genes Dev. 1988;2:517-27 pubmed
    ..In addition to suppressing loss of CDC25 function, multicopy plasmids containing SCH9 suppress the growth defects of strains lacking the RAS genes, the ..
  7. Haney S, Broach J. Cdc25p, the guanine nucleotide exchange factor for the Ras proteins of Saccharomyces cerevisiae, promotes exchange by stabilizing Ras in a nucleotide-free state. J Biol Chem. 1994;269:16541-8 pubmed
    ..These data, in conjunction with the kinetic data, are consistent with the hypothesis that Cdc25p catalyzes guanine nucleotide exchange by stabilizing a nucleotide-free intermediate of Ras. ..
  8. Roelants F, Torrance P, Thorner J. Differential roles of PDK1- and PDK2-phosphorylation sites in the yeast AGC kinases Ypk1, Pkc1 and Sch9. Microbiology. 2004;150:3289-304 pubmed
  9. Pedruzzi I, Bürckert N, Egger P, De Virgilio C. Saccharomyces cerevisiae Ras/cAMP pathway controls post-diauxic shift element-dependent transcription through the zinc finger protein Gis1. EMBO J. 2000;19:2569-79 pubmed
    ..Moreover, PDS element-driven expression, which is negatively regulated by the Ras/cAMP pathway and which is induced upon nutrient limitation, is almost entirely dependent on the presence of Gis1. ..
  10. Gelperin D, Weigle J, Nelson K, Roseboom P, Irie K, Matsumoto K, et al. 14-3-3 proteins: potential roles in vesicular transport and Ras signaling in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1995;92:11539-43 pubmed
    ..Overexpression of BMH genes also partially suppresses the temperature sensitivity of the cdc25-1 mutant, and high-copy TPK1, encoding a cAMP-dependent protein kinase, restores Bmh- yeast to viability...
  11. Maeda T, Watanabe Y, Kunitomo H, Yamamoto M. Cloning of the pka1 gene encoding the catalytic subunit of the cAMP-dependent protein kinase in Schizosaccharomyces pombe. J Biol Chem. 1994;269:9632-7 pubmed
    ..pombe has only one gene encoding it. This situation contrasts with the case of S. cerevisiae, in which three genes encode the catalytic subunits. ..
  12. Medvedik O, Lamming D, Kim K, Sinclair D. MSN2 and MSN4 link calorie restriction and TOR to sirtuin-mediated lifespan extension in Saccharomyces cerevisiae. PLoS Biol. 2007;5:e261 pubmed
    ..These findings suggest that TOR and sirtuins may be part of the same longevity pathway in higher organisms, and that they may promote genomic stability during aging. ..
  13. Kim J, Powers S. Overexpression of RPI1, a novel inhibitor of the yeast Ras-cyclic AMP pathway, down-regulates normal but not mutationally activated ras function. Mol Cell Biol. 1991;11:3894-904 pubmed
    ..We present evidence that the down-regulatory effect of RPI1 requires the presence of one of the two Ras GTPase activators, IRA1 and IRA2. ..
  14. Sadeh A, Movshovich N, Volokh M, Gheber L, Aharoni A. Fine-tuning of the Msn2/4-mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners. Mol Biol Cell. 2011;22:3127-38 pubmed publisher
    ..Such negative regulation is crucial to minimize the cost of uncontrolled stress response gene expression and ensures a high growth rate in the absence of stress. ..
  15. Tanaka K, Nakafuku M, Tamanoi F, Kaziro Y, Matsumoto K, Toh e A. IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein. Mol Cell Biol. 1990;10:4303-13 pubmed heat shock and nitrogen starvation, (ii) sporulation defects, and (iii) suppression of the lethality of the cdc25 mutant...
  16. Tanaka K, Matsumoto K, Toh e A. IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae. Mol Cell Biol. 1989;9:757-68 pubmed
    ..Disruption of the IRA1 gene suppressed the lethality of the cdc25 mutation but did not suppress the lethality of either the ras1 ras2 or the cyr1 mutations...
  17. Gross E, Goldberg D, Levitzki A. Phosphorylation of the S. cerevisiae Cdc25 in response to glucose results in its dissociation from Ras. Nature. 1992;360:762-5 pubmed
    ..The glucose signal is processed by the Cdc25/Ras/adenylyl cyclase pathway, where the role of Cdc25 is to catalyse the GDP-GTP exchange on Ras...
  18. Van Aelst L, Jans A, Thevelein J. Involvement of the CDC25 gene product in the signal transmission pathway of the glucose-induced RAS-mediated cAMP signal in the yeast Saccharomyces cerevisiae. J Gen Microbiol. 1991;137:341-9 pubmed
    ..Yeast strains without a functional CDC25 gene were deficient in basal cAMP synthesis and in the glucose-induced cAMP signal...
  19. Powers S, Gonzales E, Christensen T, Cubert J, Broek D. Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant. Cell. 1991;65:1225-31 pubmed
    By searching for genes that behave like CDC25 of S. cerevisiae in their ability to counteract a dominant-negative RAS2 mutant in a wild-type RAS-dependent manner, we have isolated a CDC25-like homolog, BUD5...
  20. Trott A, Shaner L, Morano K. The molecular chaperone Sse1 and the growth control protein kinase Sch9 collaborate to regulate protein kinase A activity in Saccharomyces cerevisiae. Genetics. 2005;170:1009-21 pubmed
    ..Together these results demonstrate that the Sse1 chaperone and the growth control kinase Sch9 independently contribute to regulation of PKA signaling. ..
  21. Cannon J, Tatchell K. Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase. Mol Cell Biol. 1987;7:2653-63 pubmed
    ..plasmids behaves like spontaneous dominant SRA3 mutations by suppressing ras2-530 (RAS2::LEU2 disruption), cdc25, and cdc35 mutations...
  22. Tripp M, Pinon R. Control of the cAMP pathway by the cell cycle start function, CDC25, in Saccharomyces cerevisiae. J Gen Microbiol. 1986;132:1143-51 pubmed
    We investigated the relationship in Saccharomyces cerevisiae between the cell cycle start function, CDC25, and two mutants defining components of the cAMP pathway...
  23. Toda T, Cameron S, Sass P, Zoller M, Wigler M. Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell. 1987;50:277-87 pubmed
    ..The amino-terminal regions show no homology to each other and are heterogeneous in length. The TPK1 gene carried on a multicopy plasmid can suppress both a temperature-sensitive ras2 gene and adenylate cyclase gene. ..
  24. Crechet J, Poullet P, Bernardi A, Fasano O, Parmeggiani A. Properties of the SDC25 C-domain, a GDP to GTP exchange factor of RAS proteins and in vitro modulation of adenylyl cyclase. J Biol Chem. 1993;268:14836-41 pubmed
    ..The SDC25 C-domain was able to stimulate the adenylyl cyclase activity of membranes from RAS2 cdc25 strains...
  25. Broek D, Toda T, Michaeli T, Levin L, Birchmeier C, Zoller M, et al. The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway. Cell. 1987;48:789-99 pubmed
    The gene corresponding to the S. cerevisiae cell division cycle mutant cdc25 has been cloned and sequenced, revealing an open reading frame encoding a protein of 1589 amino acids that contains no significant homologies with other known ..
  26. Boy Marcotte E, Ikonomi P, Jacquet M. SDC25, a dispensable Ras guanine nucleotide exchange factor of Saccharomyces cerevisiae differs from CDC25 by its regulation. Mol Biol Cell. 1996;7:529-39 pubmed
    The SDC25 gene of Saccharomyces cerevisiae is homologous to CDC25. Its 3' domain encodes a guanine nucleotide exchange factor (GEF) for Ras...
  27. Wang C, Skinner C, Easlon E, Lin S. Deleting the 14-3-3 protein Bmh1 extends life span in Saccharomyces cerevisiae by increasing stress response. Genetics. 2009;183:1373-84 pubmed publisher
    ..Deleting Bmh1 may eliminate the inhibitory effects of Bmh1 on these longevity factors and therefore extends life span. ..
  28. Garrett J. The control of morphogenesis in Saccharomyces cerevisiae by Elm1 kinase is responsive to RAS/cAMP pathway activity and tryptophan availability. Mol Microbiol. 1997;26:809-20 pubmed
    ..Overexpression of Elm1 partially rescues the ts phenotype of cdc25 and cyr1 mutants...
  29. Pescini D, Cazzaniga P, Besozzi D, Mauri G, Amigoni L, Colombo S, et al. Simulation of the Ras/cAMP/PKA pathway in budding yeast highlights the establishment of stable oscillatory states. Biotechnol Adv. 2012;30:99-107 pubmed publisher consider, in particular, the effect of the negative feedback loops on the activity of both Ira2 (a Ras-GAP) and Cdc25 (a Ras-GEF) proteins...
  30. Belotti F, Tisi R, Martegani E. The N-terminal region of the Saccharomyces cerevisiae RasGEF Cdc25 is required for nutrient-dependent cell-size regulation. Microbiology. 2006;152:1231-42 pubmed
    In the yeast Saccharomyces cerevisiae, the Cdc25/Ras/cAMP/protein kinase A (PKA) pathway plays a major role in the control of metabolism, stress resistance and proliferation, in relation to the available nutrients and conditions...
  31. Mosteller R, Park W, Broek D. Analysis of interaction between Ras and CDC25 guanine nucleotide exchange factor using yeast GAL4 two-hybrid system. Methods Enzymol. 1995;255:135-48 pubmed
    ..system can be useful for studying interactions of the wild-type and mutant forms of Ras proteins with the CDC25 guanine nucleotide exchange factor (CDC25-GEF)...
  32. Paiardi C, Belotti F, Colombo S, Tisi R, Martegani E. The large N-terminal domain of Cdc25 protein of the yeast Saccharomyces cerevisiae is required for glucose-induced Ras2 activation. FEMS Yeast Res. 2007;7:1270-5 pubmed
    The Saccharomyces cerevisiae CDC25 gene encodes a guanine nucleotide exchange factor for Ras proteins whose catalytic domain is highly homologous to Ras-guanine nucleotide exchange factors from higher eukaryotes...
  33. Marshall M, Gibbs J, Scolnick E, Sigal I. Regulatory function of the Saccharomyces cerevisiae RAS C-terminus. Mol Cell Biol. 1987;7:2309-15 pubmed
    ..Lethal CDC25 mutations were bypassed by the expression of mutant RAS1 or RAS2 proteins with activating amino acid substitutions,..
  34. Munder T, Furst P. The Saccharomyces cerevisiae CDC25 gene product binds specifically to catalytically inactive ras proteins in vivo. Mol Cell Biol. 1992;12:2091-9 pubmed
    Genetic data suggest that the yeast cell cycle control gene CDC25 is an upstream regulator of RAS2...
  35. Crechet J, Jacquet E, Bernardi A, Parmeggiani A. Analysis of the role of the hypervariable region of yeast Ras2p and its farnesylation in the interaction with exchange factors and adenylyl cyclase. J Biol Chem. 2000;275:17754-61 pubmed
    ..Whereas Ras2p farnesylation had no effect on the interaction with purified GEFs from the Cdc25 family, this modification became a strict requirement for stimulation of the nucleotide exchange on Ras using ..
  36. Robinson L, Tatchell K. TFS1: a suppressor of cdc25 mutations in Saccharomyces cerevisiae. Mol Gen Genet. 1991;230:241-50 pubmed
    The TFS1 gene of Saccharomyces cerevisiae is a dosage-dependent suppressor of cdc25 mutations. Overexpression of TFS1 does not alleviate defects of temperature-sensitive adenylyl cyclase (cdc35) or ras2 disruption mutations...
  37. Chautard H, Jacquet M, Schoentgen F, Bureaud N, Bénédetti H. Tfs1p, a member of the PEBP family, inhibits the Ira2p but not the Ira1p Ras GTPase-activating protein in Saccharomyces cerevisiae. Eukaryot Cell. 2004;3:459-70 pubmed
    ..TFS1 is a multicopy suppressor of the cdc25-1 mutation in yeast and corresponds to the so-called Ic CPY cytoplasmic inhibitor...
  38. Pérez Landero S, Sandoval Motta S, Martínez Anaya C, Yang R, Folch Mallol J, Martínez L, et al. Complex regulation of Hsf1-Skn7 activities by the catalytic subunits of PKA in Saccharomyces cerevisiae: experimental and computational evidences. BMC Syst Biol. 2015;9:42 pubmed publisher
    ..Accordingly, we found new roles in growth control and stress response for Hsf1/Skn7 when PKA activity was low (cdc25Δ cells)...
  39. Damak F, Boy Marcotte E, Le Roscouet D, Guilbaud R, Jacquet M. SDC25, a CDC25-like gene which contains a RAS-activating domain and is a dispensable gene of Saccharomyces cerevisiae. Mol Cell Biol. 1991;11:202-12 pubmed
    In the yeast Saccharomyces cerevisiae, the CDC25 gene product activates adenylate cyclase through RAS1 and RAS2 gene products...
  40. Jian D, Aili Z, Xiaojia B, Huansheng Z, Yun H. Feedback regulation of Ras2 guanine nucleotide exchange factor (Ras2-GEF) activity of Cdc25p by Cdc25p phosphorylation in the yeast Saccharomyces cerevisiae. FEBS Lett. 2010;584:4745-50 pubmed publisher
    ..These data suggest a negative feedback mechanism by which intracellular cAMP synthesis is inhibited by PKA through Cdc25p phosphorylation. ..
  41. Tisi R, Belotti F, Paiardi C, Brunetti F, Martegani E. The budding yeast RasGEF Cdc25 reveals an unexpected nuclear localization. Biochim Biophys Acta. 2008;1783:2363-74 pubmed publisher
    The mechanisms regulating the activity of Saccharomyces cerevisiae Ras-GEF Cdc25 are still largely unknown...
  42. Garreau H, Geymonat M, Renault G, Jacquet M. Membrane-anchoring domains of Cdc25p, a Saccharomyces cerevisiae ras exchange factor. Biol Cell. 1996;86:93-102 pubmed
    The CDC25 gene product from Saccharomyces cerevisiae, the prototype of the family of ras guanine nucleotide exchange factors, is expressed as a 180-kDa polypeptide, tightly bound to a membrane fraction...
  43. Schmelzle T, Beck T, Martin D, Hall M. Activation of the RAS/cyclic AMP pathway suppresses a TOR deficiency in yeast. Mol Cell Biol. 2004;24:338-51 pubmed
    ..Our findings suggest that TOR signals through the RAS/cAMP pathway, independently of TAP42/SIT4. Therefore, the RAS/cAMP pathway may be a novel TOR effector branch. ..
  44. Fujimura K, Tanaka K, Toh e A. A dominant interfering mutation in RAS1 of Saccharomyces cerevisiae. Mol Gen Genet. 1993;241:280-6 pubmed
    ..The inhibitory effect of the RAS1Ser22 gene could be overcome either by overexpression of CDC25 or by the ira2 disruption mutation...
  45. Easlon E, Tsang F, Skinner C, Wang C, Lin S. The malate-aspartate NADH shuttle components are novel metabolic longevity regulators required for calorie restriction-mediated life span extension in yeast. Genes Dev. 2008;22:931-44 pubmed publisher
    ..Together, these data suggest that CR may extend life span and ameliorate age-associated metabolic diseases by activating components of the NADH shuttles. ..
  46. McDonald C, Wagner M, Dunham M, Shin M, Ahmed N, Winter E. The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae. Genetics. 2009;181:511-23 pubmed publisher
    ..The suppressors are partial function alleles of CDC25 and CYR1, which encode the Ras GDP/GTP exchange factor and adenyl cyclase, respectively, and MDS3, which encodes a ..
  47. Deminoff S, Ramachandran V, Herman P. Distal recognition sites in substrates are required for efficient phosphorylation by the cAMP-dependent protein kinase. Genetics. 2009;182:529-39 pubmed publisher
  48. Camonis J, Jacquet M. A new RAS mutation that suppresses the CDC25 gene requirement for growth of Saccharomyces cerevisiae. Mol Cell Biol. 1988;8:2980-3 pubmed
    ..yeast Saccharomyces cerevisiae, the activation of adenylate cyclase requires the products of the RAS genes and of CDC25. We isolated several dominant extragenic suppressors of the yeast cdc25 mutation...
  49. Park W, Mosteller R, Broek D. Identification of a dominant-negative mutation in the yeast CDC25 guanine nucleotide exchange factor for Ras. Oncogene. 1997;14:831-6 pubmed
    ..latter findings suggest that the CDC25R1489E protein bound the mutant RAS2 protein thereby releasing the wild-type CDC25 protein for activation of the wild-type RAS1 protein...
  50. Chen R, Michaeli T, Van Aelst L, Ballester R. A role for the noncatalytic N terminus in the function of Cdc25, a Saccharomyces cerevisiae Ras-guanine nucleotide exchange factor. Genetics. 2000;154:1473-84 pubmed
    The Saccharomyces cerevisiae CDC25 gene encodes a guanine nucleotide exchange factor (GEF) for Ras proteins. Its catalytic domain is highly homologous to Ras-GEFs from all eukaryotes...
  51. Baroni M, Marconi G, Parrini M, Monti P, Alberghina L. In vitro interaction between Saccharomyces cerevisiae CDC25 and RAS2 proteins. Biochem Biophys Res Commun. 1992;186:467-74 pubmed
    In Saccharomyces cerevisiae the CDC25 protein is a positive regulator of RAS/cAMP pathway [1-4], enhancing the GDP-releasing rate of RAS2 protein [5]...
  52. Lai C, Boguski M, Broek D, Powers S. Influence of guanine nucleotides on complex formation between Ras and CDC25 proteins. Mol Cell Biol. 1993;13:1345-52 pubmed
    The Saccharomyces cerevisiae CDC25 gene and closely homologous genes in other eukaryotes encode guanine nucleotide exchange factors for Ras proteins...
  53. Matsuura A, Treinin M, Mitsuzawa H, Kassir Y, Uno I, Simchen G. The adenylate cyclase/protein kinase cascade regulates entry into meiosis in Saccharomyces cerevisiae through the gene IME1. EMBO J. 1990;9:3225-32 pubmed
    ..Multicopy IME1 plasmids overcome the meiotic deficiency of bcy1 and of RASval19 diploids. Double mutants ime1 cdc25 and ime1 ras2 are sporulation deficient. These results suggest that IME1 comes after the AC/PK cascade...
  54. Balciunas D, Ronne H. Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells. Mol Gen Genet. 1999;262:589-99 pubmed
    ..Instead, we find that the GIS1, GIS2 and GIS4 genes interact with the CDC25 gene, indicating a possible involvement of these genes in the RAS/cAMP signaling pathway.
  55. Powers S, O Neill K, Wigler M. Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae. Mol Cell Biol. 1989;9:390-5 pubmed
    ..The inhibitory effects of the mutant RAS2 or H-ras genes could be overcome by overexpression of CDC25, but only in the presence of wild-type RAS...
  56. Dong J, Bai X. The membrane localization of Ras2p and the association between Cdc25p and Ras2-GTP are regulated by protein kinase A (PKA) in the yeast Saccharomyces cerevisiae. FEBS Lett. 2011;585:1127-34 pubmed publisher
    ..These results suggest that Ras2p localization and Ras2-GEF activity of Cdc25p play roles in the feedback regulation of Ras2p in the Ras-cAMP pathway. ..