Gene Symbol: CDC19
Description: pyruvate kinase CDC19
Alias: PYK1, pyruvate kinase CDC19
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Portela P, Moreno S, Rossi S. Characterization of yeast pyruvate kinase 1 as a protein kinase A substrate, and specificity of the phosphorylation site sequence in the whole protein. Biochem J. 2006;396:117-26 pubmed
    b>Pyk1 (pyruvate kinase 1) from Saccharomyces cerevisiae was characterized as a substrate for PKA (protein kinase A) from bovine heart and yeast...
  2. Brazill D, Thorner J, Martin G. Mck1, a member of the glycogen synthase kinase 3 family of protein kinases, is a negative regulator of pyruvate kinase in the yeast Saccharomyces cerevisiae. J Bacteriol. 1997;179:4415-8 pubmed
    An interaction between the Saccharomyces cerevisiae protein kinase Mck1 and pyruvate kinase (Pyk1) was detected by using the two-hybrid method. Purified Mck1 was able to phosphorylate purified Pyk1 on Ser in vitro...
  3. DERMODY J, Buratowski S. Leo1 subunit of the yeast paf1 complex binds RNA and contributes to complex recruitment. J Biol Chem. 2010;285:33671-9 pubmed publisher
    ..Together, these results suggest that association of Paf1C with RNA stabilizes its localization at actively transcribed regions where it influences chromatin structure. ..
  4. Nedea E, He X, Kim M, Pootoolal J, Zhong G, Canadien V, et al. Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem. 2003;278:33000-10 pubmed
    ..small nucleolar RNA (snoRNA) genes and primarily near the polyadenylation signals of the constitutively expressed PYK1 and PMA1 genes...
  5. Li S, Swanson S, Gogol M, Florens L, Washburn M, Workman J, et al. Serine and SAM Responsive Complex SESAME Regulates Histone Modification Crosstalk by Sensing Cellular Metabolism. Mol Cell. 2015;60:408-21 pubmed publisher
    ..Here, we show that the yeast PKM2 homolog, Pyk1, is a part of a novel protein complex named SESAME (Serine-responsive SAM-containing Metabolic Enzyme complex), ..
  6. Das Bradoo S, Nguyen H, Wood J, Ricke R, Haworth J, Bielinsky A. Defects in DNA ligase I trigger PCNA ubiquitylation at Lys 107. Nat Cell Biol. 2010;12:74-9; sup pp 1-20 pubmed publisher
    ..We propose that PCNA ubiquitylation provides a 'DNA damage code' that allows cells to categorize different types of defects that arise during DNA replication. ..
  7. Saad S, Cereghetti G, Feng Y, Picotti P, Peter M, Dechant R. Reversible protein aggregation is a protective mechanism to ensure cell cycle restart after stress. Nat Cell Biol. 2017;19:1202-1213 pubmed publisher
    ..Here, we characterize stress-induced, reversible aggregation of yeast pyruvate kinase, Cdc19. Aggregation of Cdc19 is regulated by oligomerization and binding to allosteric regulators...
  8. Phan V, Ding V, Li F, Chalkley R, Burlingame A, McCormick F. The RasGAP proteins Ira2 and neurofibromin are negatively regulated by Gpb1 in yeast and ETEA in humans. Mol Cell Biol. 2010;30:2264-79 pubmed publisher
    ..These findings provide evidence for conserved ubiquitination pathways regulating the RasGAP proteins Ira2 (in yeast) and neurofibromin (in humans). ..
  9. Kus B, Gajadhar A, Stanger K, Cho R, Sun W, Rouleau N, et al. A high throughput screen to identify substrates for the ubiquitin ligase Rsp5. J Biol Chem. 2005;280:29470-8 pubmed
    ..The combination of this sensitive assay and the availability of purified substrates will enable the identification of substrates for any purified E3 enzyme. ..

More Information


  1. Lahudkar S, Durairaj G, Uprety B, Bhaumik S. A novel role for Cet1p mRNA 5'-triphosphatase in promoter proximal accumulation of RNA polymerase II in Saccharomyces cerevisiase. Genetics. 2014;196:161-76 pubmed publisher
    ..Collectively, our results demonstrate a novel role of Cet1p in regulation of promoter proximal accumulation of RNA polymerase II independently of mRNA capping activity, and hence transcription in vivo...
  2. Clifton D, Weinstock S, Fraenkel D. Glycolysis mutants in Saccharomyces cerevisiae. Genetics. 1978;88:1-11 pubmed
    ..In diploids the mutations were recessive. pyk, pgi, pfk, and gcr segregated 2:2 from their wild-type alleles. PYK hxk, PYK pfk, and PYK gcr segregrants grew on glucose. ..
  3. Rayner T, Gray J, Thorner J. Direct and novel regulation of cAMP-dependent protein kinase by Mck1p, a yeast glycogen synthase kinase-3. J Biol Chem. 2002;277:16814-22 pubmed
  4. Calvo O, Manley J. The transcriptional coactivator PC4/Sub1 has multiple functions in RNA polymerase II transcription. EMBO J. 2005;24:1009-20 pubmed
    ..Our data provide evidence that Rna15 and Sub1 are present along the length of several genes and that Sub1 facilitates elongation by influencing enzymes that modify RNAP II. ..
  5. Fang N, Chan G, Zhu M, Comyn S, Persaud A, Deshaies R, et al. Rsp5/Nedd4 is the main ubiquitin ligase that targets cytosolic misfolded proteins following heat stress. Nat Cell Biol. 2014;16:1227-37 pubmed publisher
    ..Our results support a bipartite recognition mechanism combining direct and chaperone-dependent ubiquitylation of misfolded cytosolic proteins by Rsp5. ..
  6. Finnigan G, Duvalyan A, Liao E, Sargsyan A, Thorner J. Detection of protein-protein interactions at the septin collar in Saccharomyces cerevisiae using a tripartite split-GFP system. Mol Biol Cell. 2016;27:2708-25 pubmed publisher
  7. Galello F, Portela P, Moreno S, Rossi S. Characterization of substrates that have a differential effect on Saccharomyces cerevisiae protein kinase A holoenzyme activation. J Biol Chem. 2010;285:29770-9 pubmed publisher
    ..the substrate behavior and sequence determinants around the phosphorylation site of three protein substrates, Pyk1, Pyk2, and Nth1...
  8. Benjaphokee S, Koedrith P, Auesukaree C, Asvarak T, Sugiyama M, Kaneko Y, et al. CDC19 encoding pyruvate kinase is important for high-temperature tolerance in Saccharomyces cerevisiae. N Biotechnol. 2012;29:166-76 pubmed publisher
    ..The gene CDC19, encoding pyruvate kinase, was cloned as the HTG2 gene...
  9. Jablonowski D, Fichtner L, Stark M, Schaffrath R. The yeast elongator histone acetylase requires Sit4-dependent dephosphorylation for toxin-target capacity. Mol Biol Cell. 2004;15:1459-69 pubmed
    ..Together with the findings that sit4Delta and totDelta cells phenocopy protection against zymocin and the ceramide-induced G1 block, Sit4 is functionally linked to Elongator in cell cycle events targetable by antizymotics. ..
  10. Kamber R, Shoemaker C, Denic V. Receptor-Bound Targets of Selective Autophagy Use a Scaffold Protein to Activate the Atg1 Kinase. Mol Cell. 2015;59:372-81 pubmed publisher
    ..This regulatory logic is a key similarity between selective autophagy and bulk autophagy, which is initiated by a distinct Atg1 activation mechanism during starvation. ..
  11. Sprague G. Isolation and characterization of a Saccharomyces cerevisiae mutant deficient in pyruvate kinase activity. J Bacteriol. 1977;130:232-41 pubmed
    ..the phenotype of the pyruvate kinase-deficient strain was due to a single nuclear mutation, which was designated pyk1, and preliminary genetic mapping experiments located the pyk1 locus on chromosome I, 30 centimorgans from the ade1 ..
  12. Portela P, Howell S, Moreno S, Rossi S. In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A. J Biol Chem. 2002;277:30477-87 pubmed
    Saccharomyces cerevisiae pyruvate kinase 1 (Pyk1) was demonstrated to be associated to an immunoprecipitate of yeast protein kinase A holoenzyme (HA-Tpk1.Bcy1) and to be phosphorylated in a cAMP-dependent process...
  13. Kim S, Bae S, Hahn J. Redirection of pyruvate flux toward desired metabolic pathways through substrate channeling between pyruvate kinase and pyruvate-converting enzymes in Saccharomyces cerevisiae. Sci Rep. 2016;6:24145 pubmed publisher
    ..As a platform strain for the channeling of pyruvate flux, PYK1-Coh-Myc strain was constructed in which PYK1 gene encoding pyruvate kinase is tagged with cohesin domain...
  14. Fichtner L, Frohloff F, Jablonowski D, Stark M, Schaffrath R. Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity. Mol Microbiol. 2002;45:817-26 pubmed
    ..Two-hybrid analysis showed that Tot4p also interacts with Cdc19p, suggesting that Tot4p plays an additional role in concert with Cdc19p, perhaps co-ordinating cell growth with carbon source metabolism. ..
  15. Xu Y, Zhao X, Glass D, Absalan F, Perlman D, Broach J, et al. Regulation of yeast pyruvate kinase by ultrasensitive allostery independent of phosphorylation. Mol Cell. 2012;48:52-62 pubmed publisher
    ..The main pyruvate kinase isozyme (Cdc19) is phosphorylated in response to environmental cues...