Genomes and Genes
Gene Symbol: CDC13
Species: Saccharomyces cerevisiae S288c
- Shelterin-like proteins and Yku inhibit nucleolytic processing of Saccharomyces cerevisiae telomeresDiego Bonetti
Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
PLoS Genet 6:e1000966. 2010..Thus, chromosome end degradation is controlled by telomeric proteins that specifically inhibit the action of different nucleases...
- Deregulated Ras signaling compromises DNA damage checkpoint recovery in S. cerevisiaeMatthew D Wood
Department of Pharmacology and Toxicology, Norris Cotton Cancer Center, Dartmouth Medical School, Hanover, NH, USA
Cell Cycle 9:3353-63. 2010..This work identifies a new signaling pathway that can regulate DNA damage checkpoint recovery and implicates the Ras signaling pathway as an important regulator of mitotic events...
- Interaction of Saccharomyces Cdc13p with Pol1p, Imp4p, Sir4p and Zds2p is involved in telomere replication, telomere maintenance and cell growth controlChia Ling Hsu
Institute of Biopharmaceutical Science, National Yang Ming University, Shih Pai 112, Taipei, Taiwan, Republic of China
Nucleic Acids Res 32:511-21. 2004..The function of the N-terminal 1-252 region of Cdc13p was also analyzed. Expressing Cdc13(252-924)p, which lacks amino acids 1-252 of Cdc13p, causes defects in progressive cell growth and eventually ..
- Cdc13p: a single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenanceC I Nugent
Department of Molecular and Human Genetics and Cell and Molecular Biology Program, Baylor College of Medicine, Houston, TX 77030, USA
Science 274:249-52. 1996The CDC13 gene has previously been implicated in the maintenance of telomere integrity in Saccharomyces cerevisiae. With the use of two classes of mutations, here it is shown that CDC13 has two discrete roles at the telomere...
- The role of Stn1p in Saccharomyces cerevisiae telomere capping can be separated from its interaction with Cdc13pRuben C Petreaca
Cell, Molecular, and Developmental Biology Graduate Program, Department of Cell Biology and Neuroscience, University of California, Riverside, California 92506, USA
Genetics 177:1459-74. 2007..Thus, an amino-terminal region of Stn1p is sufficient for its essential function, while a central region of Stn1p either negatively regulates the STN1 essential function or destabilizes the mutant Stn1 protein...
- Yeast Ku as a regulator of chromosomal DNA end structureS Gravel
Departement De Microbiologie et Infectiologie, Faculte de Medecine, Universite de Sherbrooke, 3001 12th Avenue Nord, Sherbrooke, Quebec QC J1H 5N4, Canada
Science 280:741-4. 1998..Because Ku-mediated DNA end joining involving telomeres would result in chromosome instability, our data also suggest that Ku has a distinct function when bound to telomeres...
- TEN1 is essential for CDC13-mediated telomere cappingLing Xu
Department of Cell Biology, University of California, Riverside, California 92521, USA
Genetics 183:793-810. 2009..In Saccharomyces cerevisiae, Cdc13 binds single-stranded G-rich telomere repeats, maintaining telomere integrity and length...
- Exo1 and Rad24 differentially regulate generation of ssDNA at telomeres of Saccharomyces cerevisiae cdc13-1 mutantsMikhajlo K Zubko
School of Clinical Medical Sciences Gerontology, University of Newcastle, Henry Wellcome Laboratory for Biogerontology Research, Newcastle upon Tyne, NE4 6BE, UK
Genetics 168:103-15. 2004..we examine the role of DNA repair and checkpoint genes in responding to unprotected telomeres in budding yeast cdc13-1 mutants...
- Cell cycle-dependent regulation of yeast telomerase by KuTimothy S Fisher
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
Nat Struct Mol Biol 11:1198-205. 2004..These data support a model in which Ku recruits telomerase to telomeres in G1 phase when telomerase is inactive and promotes telomerase-mediated telomere lengthening in late S phase...
- Reciprocal association of the budding yeast ATM-related proteins Tel1 and Mec1 with telomeres in vivoHideki Takata
Department of Geriatric Research, National Institute for Longevity Sciences, Obu, Aichi 474 8522, Japan
Mol Cell 14:515-22. 2004..Our study suggests a mechanistic similarity between telomere length regulation and DNA double-strand break repair, both of which are achieved by the direct association of PIKKs...
- Probing the mechanism of recognition of ssDNA by the Cdc13-DBDAimee M Eldridge
Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
Nucleic Acids Res 36:1624-33. 2008The Saccharomyces cerevisiae protein Cdc13 tightly and specifically binds the conserved G-rich single-stranded overhang at telomeres and plays an essential role in telomere end-protection and length regulation...
- Identification of the determinants for the specific recognition of single-strand telomeric DNA by Cdc13Aimee M Eldridge
Department of Chemistry and Biochemistry, UCB 215, University of Colorado, Boulder, Colorado 80309 0215, USA
Biochemistry 45:871-9. 2006..The telomere end-binding proteins, Cdc13 in Saccharomyces cerevisiae, Pot1 in higher eukaryotes, and TEBP in the ciliated protozoan Oxytricha nova, exhibit ..
- Inactivation of Cdc13p triggers MEC1-dependent apoptotic signals in yeastHaiyan Qi
Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
J Biol Chem 278:15136-41. 2003Inactivation of the budding yeast telomere binding protein Cdc13 results in abnormal telomeres (exposed long G-strands) and activation of the DNA damage checkpoint...
- De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaksWei Zhang
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
Genes Dev 24:502-15. 2010..Mec1 inhibits telomere healing by phosphorylating Cdc13 on its S306 residue, a phosphorylation event that suppresses Cdc13 accumulation at DSBs...
- Cdk1-dependent phosphorylation of Cdc13 coordinates telomere elongation during cell-cycle progressionShang Li
Department of Biochemistry and Biophysics, University of California, San Francisco, Box 2200, San Francisco, CA 94143 2200, USA
Cell 136:50-61. 2009..In budding yeast, Cdc13 plays an essential role in telomere length homeostasis, partly through its interactions with both the telomerase ..
- A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulatorMichael Downey
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, M5G 1X5, Canada
Cell 124:1155-68. 2006..involved in telomere capping, we carried out a genome-wide screen in Saccharomyces cerevisiae for suppressors of cdc13-1, an allele of the telomere-capping protein Cdc13...
- Histone methyltransferase Dot1 and Rad9 inhibit single-stranded DNA accumulation at DSBs and uncapped telomeresFederico Lazzaro
Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy
EMBO J 27:1502-12. 2008....
- Mrc1 protects uncapped budding yeast telomeres from exonuclease EXO1Avgi Tsolou
Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Henry Wellcome Laboratory for Biogerontology Research, Newcastle University, Newcastle upon Tyne NE4 6BE, UK
DNA Repair (Amst) 6:1607-17. 2007..Our experiments show that Mrc1 contributes to the vitality of both cdc13-1 and yku70Delta telomere capping mutants...
- Sensitivity of yeast strains with long G-tails to levels of telomere-bound telomeraseLeticia R Vega
Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
PLoS Genet 3:e105. 2007..a non-cell cycle-regulated promoter dramatically reduced viability in five strains with impaired end protection (cdc13-1, yku80Delta, yku70Delta, yku80-1, and yku80-4), all of which have longer single-strand G-tails than wild-type ..
- Cdc13 telomere capping decreases Mec1 association but does not affect Tel1 association with DNA endsYukinori Hirano
Department of Cell Biology and Molecular Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103, USA
Mol Biol Cell 18:2026-36. 2007..In budding yeast, Mec1 and Tel1 correspond to ATR and ATM, respectively. Here, we show that Cdc13-dependent telomere capping attenuates Mec1 association with DNA ends...
- Mrc1, a non-essential DNA replication protein, is required for telomere end protection following loss of capping by Cdc13, Yku or telomeraseNathalie Grandin
UMR CNRS no 5161, Ecole Normale Superieure de Lyon, IFR128 BioSciences Gerland, 46, Allee d Italie, 69364 Lyon, France
Mol Genet Genomics 277:685-99. 2007Proteins involved in telomere end protection have previously been identified. In Saccharomyces cerevisiae, Cdc13, Yku and telomerase, mainly, prevent telomere uncapping, thus providing telomere stability and avoiding degradation and ..
- The telomerase-recruitment domain of the telomere binding protein Cdc13 is regulated by Mec1p/Tel1p-dependent phosphorylationShun Fu Tseng
Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan
Nucleic Acids Res 34:6327-36. 2006..In addition, normal telomere length and growth could be restored by expressing a Cdc13-Est1p hybrid protein...
- DNA degradation at unprotected telomeres in yeast is regulated by the CDK1 (Cdc28/Clb) cell-cycle kinaseMomchil D Vodenicharov
Department of Microbiology, Faculty of Medicine, Universite de Sherbrooke, 3001 12e Ave Nord, Sherbrooke, Quebec J1H 5N4, Canada
Mol Cell 24:127-37. 2006..These results strongly suggest that after a loss of the telomere capping function, telomere-led genome instability is caused by tightly regulated cellular DNA repair attempts...
- Linear chromosome maintenance in the absence of essential telomere-capping proteinsMikhajlo K Zubko
Institute for Ageing and Health, Henry Wellcome Laboratory for Biogerontology Research, University of Newcastle, Newcastle upon Tyne, NE4 6BE, UK
Nat Cell Biol 8:734-40. 2006..3' single-stranded overhang at telomeres include Pot1 in humans and fission yeast, TEBP in Oxytricha nova and Cdc13 in budding yeast...
- Chromosome end protection plasticity revealed by Stn1p and Ten1p bypass of Cdc13pRuben C Petreaca
Graduate Program in Cell, Molecular and Developmental Biology, 5429 Boyce Hall, University of California Riverside, Riverside, CA 92521, USA
Nat Cell Biol 8:748-55. 2006..Two Cdc13-interacting proteins, Stn1p and Ten1p, are also required for viability and telomere length regulation...
- MRX protects telomeric DNA at uncapped telomeres of budding yeast cdc13-1 mutantsSteven S Foster
Institute for Ageing and Health, University of Newcastle, Henry Wellcome Laboratory for Biogerontology Research, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, UK
DNA Repair (Amst) 5:840-51. 2006..To investigate the role of MRX, we generated a conditionally degradable Rad50 protein and combined this with cdc13-1, a temperature sensitive mutation in the Cdc13 telomere capping protein...
- DNA damage checkpoint maintains CDH1 in an active state to inhibit anaphase progressionTao Zhang
Institute of Molecular and Cell Biology, A STAR Agency for Science, Technology and Research 61, Biopolis Drive, Proteos, Singapore 138673
Dev Cell 17:541-51. 2009..Hence, the DNA damage checkpoint suppresses both cohesin cleavage and spindle elongation to preserve chromosome stability...
- Telomere capping in non-dividing yeast cells requires Yku and Rap1Momchil D Vodenicharov
Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
EMBO J 29:3007-19. 2010..We show here that the essential Cdc13-Stn1-Ten1 complex is entirely dispensable for telomere protection in non-dividing cells...
- EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Delta mutantsLaura Maringele
School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
Genes Dev 16:1919-33. 2002..This link is confirmed by our demonstration that EXO1 also plays a role in ssDNA generation in cdc13-1 mutants...
- New function of CDC13 in positive telomere length regulationB Meier
Institute for Biochemistry, University of Munich LMU, D 81377 Munich, Germany
Mol Cell Biol 21:4233-45. 2001Two roles for the Saccharomyces cerevisiae Cdc13 protein at the telomere have previously been characterized: it recruits telomerase to the telomere and protects chromosome ends from degradation...
- Cdc13 cooperates with the yeast Ku proteins and Stn1 to regulate telomerase recruitmentN Grandin
Ecole Normale Superieure, UMR CNRS ENS 5665, Lyon 69364, France
Mol Cell Biol 20:8397-408. 2000The Saccharomyces cerevisiae CDC13 protein binds single-strand telomeric DNA. Here we report the isolation of new mutant alleles of CDC13 that confer either abnormal telomere lengthening or telomere shortening...
- The yeast Ku heterodimer is essential for protection of the telomere against nucleolytic and recombinational activitiesR M Polotnianka
Department of Biochemistry, Tulane University Medical Center, New Orleans, Louisiana 70112, USA
Curr Biol 8:831-4. 1998..Both Ku subunits genetically interacted with the putative single-stranded telomere-binding protein Cdc13p. We propose that Ku protects the telomere against nucleases and recombinases...
- Telomere maintenance is dependent on activities required for end repair of double-strand breaksC I Nugent
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Curr Biol 8:657-60. 1998..Ku end-binding complex defined a third telomere-associated activity, required in parallel with telomerase  and Cdc13, a protein binding the single-strand portion of telomere DNA [7,8]...
- Stn1, a new Saccharomyces cerevisiae protein, is implicated in telomere size regulation in association with Cdc13N Grandin
Yeast Cell Cycle Group, Unité Mixte de Recherche Centre National de laRecherche Scientifique Ecole Normale Supérieure UMR CNRS ENS, No 49, Lyon, France
Genes Dev 11:512-27. 1997We have isolated STN1, an essential Saccharomyces cerevisiae gene, as a suppressor of the cdc13-1 mutation. A synthetic lethal interaction between a temperature-sensitive mutant allele of STN1, stn1-13, and cdc13-1 was observed...
- Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genesT S Lendvay
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Genetics 144:1399-412. 1996..four complementation groups: the previously identified EST1 gene and three additional genes, called EST2, EST3 and EST4. Cloning of the EST2 gene demonstrated that it encodes a large, extremely basic novel protein with no motifs that ..
- The Saccharomyces CDC13 protein is a single-strand TG1-3 telomeric DNA-binding protein in vitro that affects telomere behavior in vivoJ J Lin
Department of Molecular Biology, Princeton University, NJ 08544 1014, USA
Proc Natl Acad Sci U S A 93:13760-5. 1996..Cells lacking the activity of the essential gene CDC13 display a cell cycle arrest mediated by the DNA damage sensing, RAD9 cell cycle checkpoint, presumably because ..
- Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpointB Garvik
Department of Genetics, University of Washington, Seattle 98195, USA
Mol Cell Biol 15:6128-38. 1995A cdc13 temperature-sensitive mutant of Saccharomyces cerevisiae arrests in the G2 phase of the cell cycle at the restrictive temperature as a result of DNA damage that activates the RAD9 checkpoint...
- Ten1 functions in telomere end protection and length regulation in association with Stn1 and Cdc13N Grandin
UMR CNRS ENS no 5665, Ecole Normale Superieure, 46 allee d Italie, 69364 Lyon, France
EMBO J 20:1173-83. 2001In Saccharomyces cerevisiae, Cdc13 has been proposed to mediate telomerase recruitment at telomere ends. Stn1, which associates with Cdc13 by the two-hybrid interaction, has been implicated in telomere maintenance...
- Cdc13 both positively and negatively regulates telomere replicationA Chandra
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Genes Dev 15:404-14. 2001b>Cdc13 is a single-strand telomeric DNA-binding protein that positively regulates yeast telomere replication by recruiting telomerase to chromosome termini through a site on Cdc13 that is eliminated by the cdc13-2 mutation...
- Cdc13 delivers separate complexes to the telomere for end protection and replicationE Pennock
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
Cell 104:387-96. 2001In Saccharomyces cerevisiae, the telomere binding protein Cdc13 mediates telomere replication by recruiting telomerase, and also performs an essential function in chromosome end protection...
- The SUMO-1 isopeptidase Smt4 is linked to centromeric cohesion through SUMO-1 modification of DNA topoisomerase IIJeff Bachant
Howard Hughes Medical Institute, Houston, TX 77030, USA
Mol Cell 9:1169-82. 2002..These results suggest Top2 modification by Smt3/SUMO-1 regulates a component of chromatin structure or topology required for centromeric cohesion...
- Regulation of the Bub2/Bfa1 GAP complex by Cdc5 and cell cycle checkpointsF Hu
Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Cell 107:655-65. 2001..DNA damage does not inhibit Bfa1 phosphorylation and instead causes a Rad53- and Dun1-dependent modification of Bfa1. Regulation of Bfa1 may therefore be a key step controlled by multiple checkpoint pathways to ensure a mitotic arrest...
- Cdc13 prevents telomere uncapping and Rad50-dependent homologous recombinationN Grandin
UMR CNRS ENS no 5665, Ecole Normale Superieure de Lyon, 46, Allee d Italie, 69364 Lyon, France
EMBO J 20:6127-39. 2001b>Cdc13 performs an essential function in telomere end protection in budding yeast. Here, we analyze the consequences on telomere dynamics of cdc13-induced telomeric DNA damage in proliferating cells...
- Survival and growth of yeast without telomere capping by Cdc13 in the absence of Sgs1, Exo1, and Rad9Hien Ping Ngo
Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom
PLoS Genet 6:e1001072. 2010..Telomere capping proteins, such as Cdc13 and POT1, are essential for the viability of budding yeast and mammalian cells, respectively...
- Telomere cap components influence the rate of senescence in telomerase-deficient yeast cellsShinichiro Enomoto
Department of Genetics, Cell Biology and Development, University of Minnesota, 6 170 MCB Building, 420 Washington Avenue SE, Minneapolis, MN 55455, USA
Mol Cell Biol 24:837-45. 2004..Increasing the levels of Stn1p and Ten1p in Upf+ cells is sufficient to delay senescence. In addition, cdc13-2 mutants exhibit delayed senescence rates similar to those of upfDelta cells...
- The Bfa1/Bub2 GAP complex comprises a universal checkpoint required to prevent mitotic exitY Wang
Verna and Mars McLean Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
Curr Biol 10:1379-82. 2000..b>cdc13-1 bub2 and cdc13-1 bfa1 but not cdc13-1 mad2 double mutants rebud and reduplicate their DNA at the restrictive ..
- A domain of Rad9 specifically required for activation of Chk1 in budding yeastRichard T Blankley
School of Biological Sciences, University of Manchester, G38 Stopford Building, Oxford Rd, Manchester, M13 9PT, UK
J Cell Sci 117:601-8. 2004..The Chk1 activation domain (CAD) of Rad9 is specifically important for signalling cell-cycle arrest after cdc13-1- and yku70Delta-induced telomere damage but not for tolerating ultraviolet-induced damage or inhibiting nuclease ..
- Sir proteins, Rif proteins, and Cdc13p bind Saccharomyces telomeres in vivoB D Bourns
Pathology Department, University of Washington, Seattle, Washington 98195, USA
Mol Cell Biol 18:5600-8. 1998..The data support models in which the telomere acts as an initiation site for TPE by recruiting silencing proteins to the chromosome end...
- The Saccharomyces telomere-binding protein Cdc13p interacts with both the catalytic subunit of DNA polymerase alpha and the telomerase-associated est1 proteinH Qi
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
Genes Dev 14:1777-88. 2000..Point mutations in either CDC13 or POL1 that reduced the Cdc13p-Pol1p interaction resulted in telomerase mediated telomere lengthening...
- The role of the Mre11-Rad50-Xrs2 complex in telomerase- mediated lengthening of Saccharomyces cerevisiae telomeresY Tsukamoto
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
Curr Biol 11:1328-35. 2001....
- Distinct roles for yeast Stn1 in telomere capping and telomerase inhibitionAndrea Puglisi
Department of Molecular Biology and NCCR Program Frontiers in Genetics, University of Geneva, Geneva, Switzerland
EMBO J 27:2328-39. 2008The budding yeast Cdc13, Stn1 and Ten1 (CST) proteins are proposed to function as an RPA-like complex at telomeres that protects ('caps') chromosome ends and regulates their elongation by telomerase...
- SUMOylation regulates telomere length homeostasis by targeting Cdc13Lisa E Hang
Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
Nat Struct Mol Biol 18:920-6. 2011..A main target is Cdc13, which both positively and negatively regulates telomerase and confers end protection...
- Pif1- and Exo1-dependent nucleases coordinate checkpoint activation following telomere uncappingJames M Dewar
Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle upon Tyne, Tyne and Wear, UK
EMBO J 29:4020-34. 2010..Using the cdc13-1 mutation to conditionally 'uncap' telomeres in budding yeast, we show that the telomere capping protein Cdc13 ..
- Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13Hua Gao
Salk Institute for Biological Studies, La Jolla, California 92093 0130, USA
Genetics 186:1147-59. 2010In Saccharomyces cerevisiae, association between the Est1 telomerase subunit and the telomere-binding protein Cdc13 is essential for telomerase to be recruited to its site of action...
- Budding yeast 14-3-3 proteins contribute to the robustness of the DNA damage and spindle checkpointsNathalie Grandin
IFR128 BioSciences Gerland, UMR CNRS ENS n degrees 5239, Ecole Normale Superieure de Lyon, Lyon, France
Cell Cycle 7:2749-61. 2008..These defects were additive with those conferred by the bub2 and mad2 spindle checkpoint mutations. Following cdc13-1-induced DNA damage, the 14-3-3 response was additive with those provided by the Mec1 (ATR-related)-controlled ..
- Polymerase epsilon is required to maintain replicative senescenceAbhyuday M Deshpande
Institute for Ageing and Health, Institute of Human Genetics, Centre for Life, Newcastle upon Tyne NE1 3BZ, United Kingdom
Mol Cell Biol 31:1637-45. 2011..Every time resection and resynthesis switches, a fresh signal initiates, thus preventing checkpoint adaptation and ensuring the permanent character of senescence...
- Proteins in the nutrient-sensing and DNA damage checkpoint pathways cooperate to restrain mitotic progression following DNA damageJennifer S Searle
Department of Pharmacology and Toxicology, Norris Cotton Cancer Center, Dartmouth Medical School, Hanover, New Hampshire, USA
PLoS Genet 7:e1002176. 2011..Our data indicate that there is cross-talk between the DNA damage checkpoint and the proteins that integrate nutrient and stress signals to regulate PKA...
- Mutant telomere sequences lead to impaired chromosome separation and a unique checkpoint responseJue Lin
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94143 2200, USA
Mol Biol Cell 15:1623-34. 2004..These results suggest that mutant telomere sequences elicit a checkpoint that is genetically distinct from those activated by deletion of telomerase or DNA damage...
- Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s)A Yamamoto
Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
J Cell Biol 133:99-110. 1996..of Cdc20p, another protein implicated in the degradation of mitotic cyclins; and (c) the inactivation of Cdc13 protein or gamma irradiation, two circumstances that induce a DNA-damage checkpoint...
- Hsp90 levels affect telomere length in yeastN Grandin
UMR CNRS 5665, Ecole Normale Superieure, Lyon, France
Mol Genet Genomics 265:126-34. 2001b>Cdc13 is a Saccharomyces cerevisiae protein that binds to telomeric single-stranded DNA and regulates telomerase activity. Stnl has been shown by two-hybrid analysis to form a physical complex with Cdc13...
- Genetic analysis reveals essential and non-essential amino acids within the telomeric DNA-binding interface of Cdc13pYi Chien Lin
Institute of Biochemistry and Molecular Biology, National Yang Ming University, Shih Pai 112, Taipei, Taiwan, People s Republic of China
Biochem J 403:289-95. 2007..A series of mutants within the telomere-binding domain of Cdc13p were identified that failed to complement cdc13 mutants in vivo...
- DNA damage checkpoints inhibit mitotic exit by two different mechanismsFengshan Liang
Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306, USA
Mol Cell Biol 27:5067-78. 2007..In contrast to previous data, the Rad53 pathway negatively regulates MEN independently of Cdc5, a Polo-like kinase essential for mitotic exit. Instead, a defective Rad53 pathway alleviates the inhibition of MEN by Bfa1...
- Inactivation of the Sas2 histone acetyltransferase delays senescence driven by telomere dysfunctionMarina L Kozak
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6100, USA
EMBO J 29:158-70. 2010..In contrast, sir4 deletion sped senescence and blocked the delay conferred by sas2 or sir3 deletion. We thus show that manipulation of telomere chromatin modulates senescence caused by telomere shortening...
- Exposure of single-stranded telomeric DNA causes G2/M cell cycle arrest in Saccharomyces cerevisiaeTe Ling Pang
Institutes of Biopharmaceutical Science and Biochemistry, National Yang Ming University, Taipei 112, Taiwan, Republic of China
J Biol Chem 278:9318-21. 2003..A mutant allele of CDC13, cdc13-1, causes accumulation of single-stranded TG(1-3) DNA near telomeres along with a G(2)/M cell cycle arrest ..
- The product of Saccharomyces cerevisiae WHIP/MGS1, a gene related to replication factor C genes, interacts functionally with DNA polymerase deltaD Branzei
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, 980 8578, Japan
Mol Genet Genomics 268:371-86. 2002..Possible roles of Mgs1, DNA polymerase delta, Rad18 and Mms2 in replication and replication fork restart are discussed...
- Regulation of the mitotic exit protein kinases Cdc15 and Dbf2R Visintin
Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17 233, 40 Ames Street, Cambridge MA 02139, USA
Mol Biol Cell 12:2961-74. 2001..BUB2 negatively regulates all 3 events. Loading of Cdc15 on SPBs depends on TEM1, whereas loading of Dbf2 on SPBs and activation of Dbf2 kinase depend on TEM1 and CDC15...
- A RAD9-dependent checkpoint blocks meiosis of cdc13 yeast cellsL Weber
Department of Genetics, University of Washington, Seattle 98195
Genetics 131:55-63. 1992Mutations in CDC13 have previously been found to cause cell cycle arrest of Saccharomyces cerevisiae at a stage in G2 immediately preceding the mitotic division...
- Telomere-binding and Stn1p-interacting activities are required for the essential function of Saccharomyces cerevisiae Cdc13pM J Wang
Institute of Biopharmaceutical Science, National Yang Ming University, Shih Pai, 112, Taipei, Taiwan, Republic of China
Nucleic Acids Res 28:4733-41. 2000..the telomere-binding region of Cdc13p (amino acids 451-693) was not capable of complementing the growth defects of cdc13 mutants...
- Hypermethylation of yeast telomerase RNA by the snRNA and snoRNA methyltransferase Tgs1Jacqueline Franke
Zentrum für Medizinische Biotechnologie, Universitat Duisburg Essen, Universitätsstr 5, 45117 Essen, Germany
J Cell Sci 121:3553-60. 2008..Furthermore, tgs1Delta cells displayed a shortened replicative lifespan, suggesting that the loss of the m(3)G cap of TLC1 causes premature aging...
- Characterization of the BUD31 gene of Saccharomyces cerevisiaeBarbara Masciadri
Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
Biochem Biophys Res Commun 320:1342-50. 2004..We propose that the observed phenotypes for bud31-null strain could be the result of defective splicing and indicate a first functional role for Bud3lp and its homologs...
- Ten1p promotes the telomeric DNA-binding activity of Cdc13p: implication for its function in telomere length regulationWei Qian
The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, The Graduate School of Chinese Academy of Sciences, Shanghai, China
Cell Res 19:849-63. 2009In Saccharomyces cerevisiae, the essential gene CDC13 encodes a telomeric single-stranded DNA-binding protein that interacts with Stn1p and Ten1p genetically and physically, and is required for telomere end protection and telomere length ..
- The Rad51 pathway of telomerase-independent maintenance of telomeres can amplify TG1-3 sequences in yku and cdc13 mutants of Saccharomyces cerevisiaeNathalie Grandin
Ecole Normale Superieure de Lyon, UMR CNRS 5665, 69364 Lyon, France
Mol Cell Biol 23:3721-34. 2003In the yeast Saccharomyces cerevisiae, Cdc13, Yku, and telomerase define three parallel pathways for telomere end protection that prevent chromosome instability and death by senescence...
- A genome wide analysis of the response to uncapped telomeres in budding yeast reveals a novel role for the NAD+ biosynthetic gene BNA2 in chromosome end protectionAmanda Greenall
Aging Research Laboratories, Institute for Aging and Health, Newcastle University, Newcastle upon Tyne, UK
Genome Biol 9:R146. 2008..In order to better understand the eukaryotic response to telomere damage, budding yeast strains harboring a temperature sensitive allele of an essential telomere capping gene (cdc13-1) were subjected to a transcriptomic study.
- Rad24 truncation, coupled with altered telomere structure, promotes cdc13-1 suppression in S. cerevisiaeVanessa Y Small
Department of Cell Biology and Neuroscience, University of California, Riverside, Riverside, California 92521, USA
Cell Cycle 7:3428-39. 2008Distinguishing telomeres from DNA double strand breaks is critical for genome stability. In S. cerevisiae, the Cdc13 single-strand telomere binding protein is critical for protecting chromosome ends...
- The coordination of centromere replication, spindle formation, and kinetochore-microtubule interaction in budding yeastHong Liu
Department of Biological Sciences, Florida State University, Tallahassee, Florida, United States of America
PLoS Genet 4:e1000262. 2008..Therefore, the shorter spindle in S-phase cells is likely to facilitate proper chromosome-microtubule interaction...
- Inhibition of yeast telomerase action by the telomeric ssDNA-binding protein, Cdc13pDavid C Zappulla
Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309, USA
Nucleic Acids Res 37:354-67. 2009....
- Degradation-mediated protein quality control in the nucleusRichard G Gardner
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Cell 120:803-15. 2005..We propose that San1p-mediated degradation acts as the last line of proteolytic defense against the deleterious accumulation of aberrant proteins in the nucleus and that analogous systems exist in other eukaryotes...
- Rapid Cdc13 turnover and telomere length homeostasis are controlled by Cdk1-mediated phosphorylation of Cdc13Shun Fu Tseng
Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan
Nucleic Acids Res 37:3602-11. 2009Budding yeast telomerase is mainly activated by Tel1/Mec1 (yeast ATM/ATR) on Cdc13 from late S to G2 phase of the cell cycle...
- STM1, a gene which encodes a guanine quadruplex binding protein, interacts with CDC13 in Saccharomyces cerevisiaeN Hayashi
Department of Molecular Oncology, Cancer Research Institute, Kanazawa University, 13 1 Takara machi, Kanazawa, Ishikawa 920 0934, Japan
Mol Genet Genomics 267:806-13. 2002The CDC13 gene encodes a protein that binds to the G-rich single-strand at yeast telomeres, and serves as a regulator of telomere replication...
- Cdc20, a beta-transducin homologue, links RAD9-mediated G2/M checkpoint control to mitosis in Saccharomyces cerevisiaeH H Lim
Institute of Molecular and Cell Biology, National University of Singapore, Singapore
Mol Gen Genet 253:138-48. 1996..These genes also prevent entry into mitosis of a temperature-sensitive mutant, cdc13, that accumulates chromosome damage at 37 degrees C...
- Dynamic regulation of single-stranded telomeres in Saccharomyces cerevisiaeStephanie Smith
Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Genetics 178:693-701. 2008....