CDC13

Summary

Gene Symbol: CDC13
Description: Cdc13p
Alias: EST4
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaks
    Wei Zhang
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Genes Dev 24:502-15. 2010
  2. pmc Ten1 functions in telomere end protection and length regulation in association with Stn1 and Cdc13
    N Grandin
    UMR CNRS ENS no 5665, Ecole Normale Superieure, 46 allee d Italie, 69364 Lyon, France
    EMBO J 20:1173-83. 2001
  3. pmc Rif1 supports the function of the CST complex in yeast telomere capping
    Savani Anbalagan
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    PLoS Genet 7:e1002024. 2011
  4. pmc Telomere capping in non-dividing yeast cells requires Yku and Rap1
    Momchil D Vodenicharov
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
    EMBO J 29:3007-19. 2010
  5. pmc TEN1 is essential for CDC13-mediated telomere capping
    Ling Xu
    Department of Cell Biology, University of California, Riverside, California 92521, USA
    Genetics 183:793-810. 2009
  6. pmc Polymerase epsilon is required to maintain replicative senescence
    Abhyuday M Deshpande
    Institute for Ageing and Health, Institute of Human Genetics, Centre for Life, Newcastle upon Tyne NE1 3BZ, United Kingdom
    Mol Cell Biol 31:1637-45. 2011
  7. ncbi Chromosome end protection plasticity revealed by Stn1p and Ten1p bypass of Cdc13p
    Ruben C Petreaca
    Graduate Program in Cell, Molecular and Developmental Biology, 5429 Boyce Hall, University of California Riverside, Riverside, CA 92521, USA
    Nat Cell Biol 8:748-55. 2006
  8. pmc Cdk1-dependent phosphorylation of Cdc13 coordinates telomere elongation during cell-cycle progression
    Shang Li
    Department of Biochemistry and Biophysics, University of California, San Francisco, Box 2200, San Francisco, CA 94143 2200, USA
    Cell 136:50-61. 2009
  9. ncbi Mrc1, a non-essential DNA replication protein, is required for telomere end protection following loss of capping by Cdc13, Yku or telomerase
    Nathalie Grandin
    UMR CNRS no 5161, Ecole Normale Superieure de Lyon, IFR128 BioSciences Gerland, 46, Allee d Italie, 69364 Lyon, France
    Mol Genet Genomics 277:685-99. 2007
  10. ncbi A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator
    Michael Downey
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, M5G 1X5, Canada
    Cell 124:1155-68. 2006

Scientific Experts

  • Nathalie Grandin
  • Brian Luke
  • Naoyuki Hayashi
  • Haiyan Qi
  • David C Zappulla
  • David Lydall
  • Mikhajlo K Zubko
  • Ruben C Petreaca
  • Laura Maringele
  • Jing Jer Lin
  • Momchil D Vodenicharov
  • Constance I Nugent
  • Shun Fu Tseng
  • Aimee M Eldridge
  • Yuan Xue
  • Savani Anbalagan
  • Matthew D Wood
  • Diego Bonetti
  • Virginia A Zakian
  • Ling Xu
  • Yi Chien Lin
  • Fengshan Liang
  • Yun Wu
  • Sandrine Guillard
  • Timothy M Tucey
  • Victoria Lundblad
  • Abhyuday M Deshpande
  • Lisa E Hang
  • V Lundblad
  • Jennifer S Searle
  • Giovanna Lucchini
  • Maria Pia Longhese
  • Yolanda Sanchez
  • Chia Ling Hsu
  • Wei Zhang
  • Hien Ping Ngo
  • Marina L Kozak
  • James M Dewar
  • Hua Gao
  • C I Nugent
  • Wei Qian
  • Tao Zhang
  • Shang Li
  • Jacqueline Franke
  • Stephanie Smith
  • Andrea Puglisi
  • Daniel Durocher
  • Hong Liu
  • Raymund J Wellinger
  • Federico Lazzaro
  • Vanessa Y Small
  • Amanda Greenall
  • Yanchang Wang
  • Shu Chun Teng
  • Deborah S Wuttke
  • Charles Chuang
  • Avgi Tsolou
  • Leticia R Vega
  • Yukinori Hirano
  • Huan Chih Chiu
  • Elizabeth H Blackburn
  • Steven S Foster
  • Michael Downey
  • F Hu
  • Y Wang
  • Richard G Gardner
  • Timothy S Fisher
  • Jue Lin
  • Shinichiro Enomoto
  • Richard T Blankley
  • Hideki Takata
  • Barbara Masciadri
  • Te Ling Pang
  • V A Zakian
  • D Branzei
  • Jeff Bachant
  • David H Perlman
  • Benjamin A Garcia
  • Peter A DiMaggio
  • J J Lin
  • B Meier
  • R Visintin
  • E Pennock
  • A Chandra
  • Y Tsukamoto
  • Xiaolan Zhao
  • S J Elledge
  • Vasil Raykov
  • Michael D Rushton
  • Xianpeng Liu

Detail Information

Publications88

  1. pmc De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaks
    Wei Zhang
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Genes Dev 24:502-15. 2010
    ..Mec1 inhibits telomere healing by phosphorylating Cdc13 on its S306 residue, a phosphorylation event that suppresses Cdc13 accumulation at DSBs...
  2. pmc Ten1 functions in telomere end protection and length regulation in association with Stn1 and Cdc13
    N Grandin
    UMR CNRS ENS no 5665, Ecole Normale Superieure, 46 allee d Italie, 69364 Lyon, France
    EMBO J 20:1173-83. 2001
    In Saccharomyces cerevisiae, Cdc13 has been proposed to mediate telomerase recruitment at telomere ends. Stn1, which associates with Cdc13 by the two-hybrid interaction, has been implicated in telomere maintenance...
  3. pmc Rif1 supports the function of the CST complex in yeast telomere capping
    Savani Anbalagan
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    PLoS Genet 7:e1002024. 2011
    Telomere integrity in budding yeast depends on the CST (Cdc13-Stn1-Ten1) and shelterin-like (Rap1-Rif1-Rif2) complexes, which are thought to act independently from each other...
  4. pmc Telomere capping in non-dividing yeast cells requires Yku and Rap1
    Momchil D Vodenicharov
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
    EMBO J 29:3007-19. 2010
    ..We show here that the essential Cdc13-Stn1-Ten1 complex is entirely dispensable for telomere protection in non-dividing cells...
  5. pmc TEN1 is essential for CDC13-mediated telomere capping
    Ling Xu
    Department of Cell Biology, University of California, Riverside, California 92521, USA
    Genetics 183:793-810. 2009
    ..In Saccharomyces cerevisiae, Cdc13 binds single-stranded G-rich telomere repeats, maintaining telomere integrity and length...
  6. pmc Polymerase epsilon is required to maintain replicative senescence
    Abhyuday M Deshpande
    Institute for Ageing and Health, Institute of Human Genetics, Centre for Life, Newcastle upon Tyne NE1 3BZ, United Kingdom
    Mol Cell Biol 31:1637-45. 2011
    ..Every time resection and resynthesis switches, a fresh signal initiates, thus preventing checkpoint adaptation and ensuring the permanent character of senescence...
  7. ncbi Chromosome end protection plasticity revealed by Stn1p and Ten1p bypass of Cdc13p
    Ruben C Petreaca
    Graduate Program in Cell, Molecular and Developmental Biology, 5429 Boyce Hall, University of California Riverside, Riverside, CA 92521, USA
    Nat Cell Biol 8:748-55. 2006
    ..Two Cdc13-interacting proteins, Stn1p and Ten1p, are also required for viability and telomere length regulation...
  8. pmc Cdk1-dependent phosphorylation of Cdc13 coordinates telomere elongation during cell-cycle progression
    Shang Li
    Department of Biochemistry and Biophysics, University of California, San Francisco, Box 2200, San Francisco, CA 94143 2200, USA
    Cell 136:50-61. 2009
    ..In budding yeast, Cdc13 plays an essential role in telomere length homeostasis, partly through its interactions with both the telomerase ..
  9. ncbi Mrc1, a non-essential DNA replication protein, is required for telomere end protection following loss of capping by Cdc13, Yku or telomerase
    Nathalie Grandin
    UMR CNRS no 5161, Ecole Normale Superieure de Lyon, IFR128 BioSciences Gerland, 46, Allee d Italie, 69364 Lyon, France
    Mol Genet Genomics 277:685-99. 2007
    Proteins involved in telomere end protection have previously been identified. In Saccharomyces cerevisiae, Cdc13, Yku and telomerase, mainly, prevent telomere uncapping, thus providing telomere stability and avoiding degradation and ..
  10. ncbi A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator
    Michael Downey
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, M5G 1X5, Canada
    Cell 124:1155-68. 2006
    ..involved in telomere capping, we carried out a genome-wide screen in Saccharomyces cerevisiae for suppressors of cdc13-1, an allele of the telomere-capping protein Cdc13...
  11. pmc Pif1- and Exo1-dependent nucleases coordinate checkpoint activation following telomere uncapping
    James M Dewar
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Newcastle upon Tyne, Tyne and Wear, UK
    EMBO J 29:4020-34. 2010
    ..Using the cdc13-1 mutation to conditionally 'uncap' telomeres in budding yeast, we show that the telomere capping protein Cdc13 ..
  12. pmc Survival and growth of yeast without telomere capping by Cdc13 in the absence of Sgs1, Exo1, and Rad9
    Hien Ping Ngo
    Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom
    PLoS Genet 6:e1001072. 2010
    ..Telomere capping proteins, such as Cdc13 and POT1, are essential for the viability of budding yeast and mammalian cells, respectively...
  13. ncbi Cdc13p: a single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenance
    C I Nugent
    Department of Molecular and Human Genetics and Cell and Molecular Biology Program, Baylor College of Medicine, Houston, TX 77030, USA
    Science 274:249-52. 1996
    The CDC13 gene has previously been implicated in the maintenance of telomere integrity in Saccharomyces cerevisiae. With the use of two classes of mutations, here it is shown that CDC13 has two discrete roles at the telomere...
  14. pmc A novel checkpoint and RPA inhibitory pathway regulated by Rif1
    Yuan Xue
    Institute for Ageing and Health, Newcastle University, Newcastle upon Tyne, United Kingdom
    PLoS Genet 7:e1002417. 2011
    ..Our work has important implications for understanding the checkpoint and RPA-dependent DNA-damage responses in eukaryotic cells...
  15. pmc Distinct roles for yeast Stn1 in telomere capping and telomerase inhibition
    Andrea Puglisi
    Department of Molecular Biology and NCCR Program Frontiers in Genetics, University of Geneva, Geneva, Switzerland
    EMBO J 27:2328-39. 2008
    The budding yeast Cdc13, Stn1 and Ten1 (CST) proteins are proposed to function as an RPA-like complex at telomeres that protects ('caps') chromosome ends and regulates their elongation by telomerase...
  16. pmc Sir proteins, Rif proteins, and Cdc13p bind Saccharomyces telomeres in vivo
    B D Bourns
    Pathology Department, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Biol 18:5600-8. 1998
    ..The data support models in which the telomere acts as an initiation site for TPE by recruiting silencing proteins to the chromosome end...
  17. pmc The Saccharomyces telomere-binding protein Cdc13p interacts with both the catalytic subunit of DNA polymerase alpha and the telomerase-associated est1 protein
    H Qi
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    Genes Dev 14:1777-88. 2000
    ..Point mutations in either CDC13 or POL1 that reduced the Cdc13p-Pol1p interaction resulted in telomerase mediated telomere lengthening...
  18. ncbi Yeast Ku as a regulator of chromosomal DNA end structure
    S Gravel
    Departement De Microbiologie et Infectiologie, Faculte de Medecine, Universite de Sherbrooke, 3001 12th Avenue Nord, Sherbrooke, Quebec QC J1H 5N4, Canada
    Science 280:741-4. 1998
    ..Because Ku-mediated DNA end joining involving telomeres would result in chromosome instability, our data also suggest that Ku has a distinct function when bound to telomeres...
  19. ncbi The Bfa1/Bub2 GAP complex comprises a universal checkpoint required to prevent mitotic exit
    Y Wang
    Verna and Mars McLean Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Biol 10:1379-82. 2000
    ..b>cdc13-1 bub2 and cdc13-1 bfa1 but not cdc13-1 mad2 double mutants rebud and reduplicate their DNA at the restrictive ..
  20. pmc Telomere cap components influence the rate of senescence in telomerase-deficient yeast cells
    Shinichiro Enomoto
    Department of Genetics, Cell Biology and Development, University of Minnesota, 6 170 MCB Building, 420 Washington Avenue SE, Minneapolis, MN 55455, USA
    Mol Cell Biol 24:837-45. 2004
    ..Increasing the levels of Stn1p and Ten1p in Upf+ cells is sufficient to delay senescence. In addition, cdc13-2 mutants exhibit delayed senescence rates similar to those of upfDelta cells...
  21. pmc Shelterin-like proteins and Yku inhibit nucleolytic processing of Saccharomyces cerevisiae telomeres
    Diego Bonetti
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
    PLoS Genet 6:e1000966. 2010
    ..Thus, chromosome end degradation is controlled by telomeric proteins that specifically inhibit the action of different nucleases...
  22. pmc Interaction of Saccharomyces Cdc13p with Pol1p, Imp4p, Sir4p and Zds2p is involved in telomere replication, telomere maintenance and cell growth control
    Chia Ling Hsu
    Institute of Biopharmaceutical Science, National Yang Ming University, Shih Pai 112, Taipei, Taiwan, Republic of China
    Nucleic Acids Res 32:511-21. 2004
    ..The function of the N-terminal 1-252 region of Cdc13p was also analyzed. Expressing Cdc13(252-924)p, which lacks amino acids 1-252 of Cdc13p, causes defects in progressive cell growth and eventually ..
  23. ncbi The role of the Mre11-Rad50-Xrs2 complex in telomerase- mediated lengthening of Saccharomyces cerevisiae telomeres
    Y Tsukamoto
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Curr Biol 11:1328-35. 2001
    ....
  24. pmc Deregulated Ras signaling compromises DNA damage checkpoint recovery in S. cerevisiae
    Matthew D Wood
    Department of Pharmacology and Toxicology, Norris Cotton Cancer Center, Dartmouth Medical School, Hanover, NH, USA
    Cell Cycle 9:3353-63. 2010
    ..This work identifies a new signaling pathway that can regulate DNA damage checkpoint recovery and implicates the Ras signaling pathway as an important regulator of mitotic events...
  25. pmc RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast
    R Gardner
    Department of Molecular and Cellular Biology, The University of Arizona, PO Box 21016, Tucson, AZ 85721 0106, USA
    EMBO J 18:3173-85. 1999
    ..Each pathway independently contributes approximately 50% to G2/M arrest, effects demonstrable after cdc13-induced damage or a double-stranded break inflicted by the HO endonuclease...
  26. pmc Exo1 and Rad24 differentially regulate generation of ssDNA at telomeres of Saccharomyces cerevisiae cdc13-1 mutants
    Mikhajlo K Zubko
    School of Clinical Medical Sciences Gerontology, University of Newcastle, Henry Wellcome Laboratory for Biogerontology Research, Newcastle upon Tyne, NE4 6BE, UK
    Genetics 168:103-15. 2004
    ..we examine the role of DNA repair and checkpoint genes in responding to unprotected telomeres in budding yeast cdc13-1 mutants...
  27. doi DNA damage checkpoint maintains CDH1 in an active state to inhibit anaphase progression
    Tao Zhang
    Institute of Molecular and Cell Biology, A STAR Agency for Science, Technology and Research 61, Biopolis Drive, Proteos, Singapore 138673
    Dev Cell 17:541-51. 2009
    ..Hence, the DNA damage checkpoint suppresses both cohesin cleavage and spindle elongation to preserve chromosome stability...
  28. ncbi Cell cycle-dependent regulation of yeast telomerase by Ku
    Timothy S Fisher
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Nat Struct Mol Biol 11:1198-205. 2004
    ..These data support a model in which Ku recruits telomerase to telomeres in G1 phase when telomerase is inactive and promotes telomerase-mediated telomere lengthening in late S phase...
  29. ncbi MRX protects telomeric DNA at uncapped telomeres of budding yeast cdc13-1 mutants
    Steven S Foster
    Institute for Ageing and Health, University of Newcastle, Henry Wellcome Laboratory for Biogerontology Research, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, UK
    DNA Repair (Amst) 5:840-51. 2006
    ..To investigate the role of MRX, we generated a conditionally degradable Rad50 protein and combined this with cdc13-1, a temperature sensitive mutation in the Cdc13 telomere capping protein...
  30. pmc Sensitivity of yeast strains with long G-tails to levels of telomere-bound telomerase
    Leticia R Vega
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Genet 3:e105. 2007
    ..a non-cell cycle-regulated promoter dramatically reduced viability in five strains with impaired end protection (cdc13-1, yku80Delta, yku70Delta, yku80-1, and yku80-4), all of which have longer single-strand G-tails than wild-type ..
  31. ncbi Linear chromosome maintenance in the absence of essential telomere-capping proteins
    Mikhajlo K Zubko
    Institute for Ageing and Health, Henry Wellcome Laboratory for Biogerontology Research, University of Newcastle, Newcastle upon Tyne, NE4 6BE, UK
    Nat Cell Biol 8:734-40. 2006
    ..3' single-stranded overhang at telomeres include Pot1 in humans and fission yeast, TEBP in Oxytricha nova and Cdc13 in budding yeast...
  32. ncbi DNA degradation at unprotected telomeres in yeast is regulated by the CDK1 (Cdc28/Clb) cell-cycle kinase
    Momchil D Vodenicharov
    Department of Microbiology, Faculty of Medicine, Universite de Sherbrooke, 3001 12e Ave Nord, Sherbrooke, Quebec J1H 5N4, Canada
    Mol Cell 24:127-37. 2006
    ..These results strongly suggest that after a loss of the telomere capping function, telomere-led genome instability is caused by tightly regulated cellular DNA repair attempts...
  33. pmc Probing the mechanism of recognition of ssDNA by the Cdc13-DBD
    Aimee M Eldridge
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
    Nucleic Acids Res 36:1624-33. 2008
    The Saccharomyces cerevisiae protein Cdc13 tightly and specifically binds the conserved G-rich single-stranded overhang at telomeres and plays an essential role in telomere end-protection and length regulation...
  34. pmc Mrc1 protects uncapped budding yeast telomeres from exonuclease EXO1
    Avgi Tsolou
    Centre for Integrated Systems Biology of Ageing and Nutrition, Institute for Ageing and Health, Henry Wellcome Laboratory for Biogerontology Research, Newcastle University, Newcastle upon Tyne NE4 6BE, UK
    DNA Repair (Amst) 6:1607-17. 2007
    ..Our experiments show that Mrc1 contributes to the vitality of both cdc13-1 and yku70Delta telomere capping mutants...
  35. pmc Cdc13 telomere capping decreases Mec1 association but does not affect Tel1 association with DNA ends
    Yukinori Hirano
    Department of Cell Biology and Molecular Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103, USA
    Mol Biol Cell 18:2026-36. 2007
    ..In budding yeast, Mec1 and Tel1 correspond to ATR and ATM, respectively. Here, we show that Cdc13-dependent telomere capping attenuates Mec1 association with DNA ends...
  36. pmc The telomerase-recruitment domain of the telomere binding protein Cdc13 is regulated by Mec1p/Tel1p-dependent phosphorylation
    Shun Fu Tseng
    Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan
    Nucleic Acids Res 34:6327-36. 2006
    ..In addition, normal telomere length and growth could be restored by expressing a Cdc13-Est1p hybrid protein...
  37. ncbi The SUMO-1 isopeptidase Smt4 is linked to centromeric cohesion through SUMO-1 modification of DNA topoisomerase II
    Jeff Bachant
    Howard Hughes Medical Institute, Houston, TX 77030, USA
    Mol Cell 9:1169-82. 2002
    ..These results suggest Top2 modification by Smt3/SUMO-1 regulates a component of chromatin structure or topology required for centromeric cohesion...
  38. pmc New function of CDC13 in positive telomere length regulation
    B Meier
    Institute for Biochemistry, University of Munich LMU, D 81377 Munich, Germany
    Mol Cell Biol 21:4233-45. 2001
    Two roles for the Saccharomyces cerevisiae Cdc13 protein at the telomere have previously been characterized: it recruits telomerase to the telomere and protects chromosome ends from degradation...
  39. ncbi The yeast Ku heterodimer is essential for protection of the telomere against nucleolytic and recombinational activities
    R M Polotnianka
    Department of Biochemistry, Tulane University Medical Center, New Orleans, Louisiana 70112, USA
    Curr Biol 8:831-4. 1998
    ..Both Ku subunits genetically interacted with the putative single-stranded telomere-binding protein Cdc13p. We propose that Ku protects the telomere against nucleases and recombinases...
  40. ncbi Stn1, a new Saccharomyces cerevisiae protein, is implicated in telomere size regulation in association with Cdc13
    N Grandin
    Yeast Cell Cycle Group, Unité Mixte de Recherche Centre National de laRecherche Scientifique Ecole Normale Supérieure UMR CNRS ENS, No 49, Lyon, France
    Genes Dev 11:512-27. 1997
    We have isolated STN1, an essential Saccharomyces cerevisiae gene, as a suppressor of the cdc13-1 mutation. A synthetic lethal interaction between a temperature-sensitive mutant allele of STN1, stn1-13, and cdc13-1 was observed...
  41. pmc Cdc13 prevents telomere uncapping and Rad50-dependent homologous recombination
    N Grandin
    UMR CNRS ENS no 5665, Ecole Normale Superieure de Lyon, 46, Allee d Italie, 69364 Lyon, France
    EMBO J 20:6127-39. 2001
    b>Cdc13 performs an essential function in telomere end protection in budding yeast. Here, we analyze the consequences on telomere dynamics of cdc13-induced telomeric DNA damage in proliferating cells...
  42. ncbi Regulation of the Bub2/Bfa1 GAP complex by Cdc5 and cell cycle checkpoints
    F Hu
    Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 107:655-65. 2001
    ..DNA damage does not inhibit Bfa1 phosphorylation and instead causes a Rad53- and Dun1-dependent modification of Bfa1. Regulation of Bfa1 may therefore be a key step controlled by multiple checkpoint pathways to ensure a mitotic arrest...
  43. pmc Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes
    T S Lendvay
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genetics 144:1399-412. 1996
    ..four complementation groups: the previously identified EST1 gene and three additional genes, called EST2, EST3 and EST4. Cloning of the EST2 gene demonstrated that it encodes a large, extremely basic novel protein with no motifs that ..
  44. pmc EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Delta mutants
    Laura Maringele
    School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
    Genes Dev 16:1919-33. 2002
    ..This link is confirmed by our demonstration that EXO1 also plays a role in ssDNA generation in cdc13-1 mutants...
  45. pmc The Saccharomyces CDC13 protein is a single-strand TG1-3 telomeric DNA-binding protein in vitro that affects telomere behavior in vivo
    J J Lin
    Department of Molecular Biology, Princeton University, NJ 08544 1014, USA
    Proc Natl Acad Sci U S A 93:13760-5. 1996
    ..Cells lacking the activity of the essential gene CDC13 display a cell cycle arrest mediated by the DNA damage sensing, RAD9 cell cycle checkpoint, presumably because ..
  46. pmc Cdc13 both positively and negatively regulates telomere replication
    A Chandra
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 15:404-14. 2001
    b>Cdc13 is a single-strand telomeric DNA-binding protein that positively regulates yeast telomere replication by recruiting telomerase to chromosome termini through a site on Cdc13 that is eliminated by the cdc13-2 mutation...
  47. ncbi Cdc13 delivers separate complexes to the telomere for end protection and replication
    E Pennock
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 104:387-96. 2001
    In Saccharomyces cerevisiae, the telomere binding protein Cdc13 mediates telomere replication by recruiting telomerase, and also performs an essential function in chromosome end protection...
  48. ncbi Inactivation of Cdc13p triggers MEC1-dependent apoptotic signals in yeast
    Haiyan Qi
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 278:15136-41. 2003
    Inactivation of the budding yeast telomere binding protein Cdc13 results in abnormal telomeres (exposed long G-strands) and activation of the DNA damage checkpoint...
  49. pmc Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint
    B Garvik
    Department of Genetics, University of Washington, Seattle 98195, USA
    Mol Cell Biol 15:6128-38. 1995
    A cdc13 temperature-sensitive mutant of Saccharomyces cerevisiae arrests in the G2 phase of the cell cycle at the restrictive temperature as a result of DNA damage that activates the RAD9 checkpoint...
  50. ncbi Reciprocal association of the budding yeast ATM-related proteins Tel1 and Mec1 with telomeres in vivo
    Hideki Takata
    Department of Geriatric Research, National Institute for Longevity Sciences, Obu, Aichi 474 8522, Japan
    Mol Cell 14:515-22. 2004
    ..Our study suggests a mechanistic similarity between telomere length regulation and DNA double-strand break repair, both of which are achieved by the direct association of PIKKs...
  51. pmc The role of Stn1p in Saccharomyces cerevisiae telomere capping can be separated from its interaction with Cdc13p
    Ruben C Petreaca
    Cell, Molecular, and Developmental Biology Graduate Program, Department of Cell Biology and Neuroscience, University of California, Riverside, California 92506, USA
    Genetics 177:1459-74. 2007
    ..Thus, an amino-terminal region of Stn1p is sufficient for its essential function, while a central region of Stn1p either negatively regulates the STN1 essential function or destabilizes the mutant Stn1 protein...
  52. ncbi Telomere maintenance is dependent on activities required for end repair of double-strand breaks
    C I Nugent
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Biol 8:657-60. 1998
    ..Ku end-binding complex defined a third telomere-associated activity, required in parallel with telomerase [6] and Cdc13, a protein binding the single-strand portion of telomere DNA [7,8]...
  53. pmc Histone methyltransferase Dot1 and Rad9 inhibit single-stranded DNA accumulation at DSBs and uncapped telomeres
    Federico Lazzaro
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy
    EMBO J 27:1502-12. 2008
    ....
  54. pmc Cdc13 cooperates with the yeast Ku proteins and Stn1 to regulate telomerase recruitment
    N Grandin
    Ecole Normale Superieure, UMR CNRS ENS 5665, Lyon 69364, France
    Mol Cell Biol 20:8397-408. 2000
    The Saccharomyces cerevisiae CDC13 protein binds single-strand telomeric DNA. Here we report the isolation of new mutant alleles of CDC13 that confer either abnormal telomere lengthening or telomere shortening...
  55. pmc Identification of the determinants for the specific recognition of single-strand telomeric DNA by Cdc13
    Aimee M Eldridge
    Department of Chemistry and Biochemistry, UCB 215, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 45:871-9. 2006
    ..The telomere end-binding proteins, Cdc13 in Saccharomyces cerevisiae, Pot1 in higher eukaryotes, and TEBP in the ciliated protozoan Oxytricha nova, exhibit ..
  56. ncbi A domain of Rad9 specifically required for activation of Chk1 in budding yeast
    Richard T Blankley
    School of Biological Sciences, University of Manchester, G38 Stopford Building, Oxford Rd, Manchester, M13 9PT, UK
    J Cell Sci 117:601-8. 2004
    ..The Chk1 activation domain (CAD) of Rad9 is specifically important for signalling cell-cycle arrest after cdc13-1- and yku70Delta-induced telomere damage but not for tolerating ultraviolet-induced damage or inhibiting nuclease ..
  57. pmc Novel phosphorylation sites in the S. cerevisiae Cdc13 protein reveal new targets for telomere length regulation
    Yun Wu
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, United States
    J Proteome Res 12:316-27. 2013
    The S. cerevisiae Cdc13 is a multifunctional protein with key roles in regulation of telomerase, telomere end protection, and conventional telomere replication, all of which are cell cycle-regulated processes...
  58. doi RPA provides checkpoint-independent cell cycle arrest and prevents recombination at uncapped telomeres of Saccharomyces cerevisiae
    Nathalie Grandin
    UMR CNRS 5239, Ecole Normale Superieure de Lyon, IFR128 BioSciences Gerland, 69364 Lyon, France
    DNA Repair (Amst) 12:212-26. 2013
    ..this checkpoint but nevertheless exhibit deregulation of cell cycle control upon telomere uncapping induced by the cdc13-1 mutation...
  59. doi A yeast telomerase complex containing the Est1 recruitment protein is assembled early in the cell cycle
    Timothy M Tucey
    Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Biochemistry 52:1131-3. 2013
    ..We also report a biochemical interaction between Est1 and the telomere binding protein Cdc13 that recapitulates the previously observed genetic relationship between EST1 and CDC13...
  60. pmc Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13
    Hua Gao
    Salk Institute for Biological Studies, La Jolla, California 92093 0130, USA
    Genetics 186:1147-59. 2010
    In Saccharomyces cerevisiae, association between the Est1 telomerase subunit and the telomere-binding protein Cdc13 is essential for telomerase to be recruited to its site of action...
  61. pmc SUMOylation regulates telomere length homeostasis by targeting Cdc13
    Lisa E Hang
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Struct Mol Biol 18:920-6. 2011
    ..A main target is Cdc13, which both positively and negatively regulates telomerase and confers end protection...
  62. pmc Proteins in the nutrient-sensing and DNA damage checkpoint pathways cooperate to restrain mitotic progression following DNA damage
    Jennifer S Searle
    Department of Pharmacology and Toxicology, Norris Cotton Cancer Center, Dartmouth Medical School, Hanover, New Hampshire, USA
    PLoS Genet 7:e1002176. 2011
    ..Our data indicate that there is cross-talk between the DNA damage checkpoint and the proteins that integrate nutrient and stress signals to regulate PKA...
  63. ncbi Hsp90 levels affect telomere length in yeast
    N Grandin
    UMR CNRS 5665, Ecole Normale Superieure, Lyon, France
    Mol Genet Genomics 265:126-34. 2001
    b>Cdc13 is a Saccharomyces cerevisiae protein that binds to telomeric single-stranded DNA and regulates telomerase activity. Stnl has been shown by two-hybrid analysis to form a physical complex with Cdc13...
  64. pmc DNA damage checkpoints inhibit mitotic exit by two different mechanisms
    Fengshan Liang
    Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306, USA
    Mol Cell Biol 27:5067-78. 2007
    ..In contrast to previous data, the Rad53 pathway negatively regulates MEN independently of Cdc5, a Polo-like kinase essential for mitotic exit. Instead, a defective Rad53 pathway alleviates the inhibition of MEN by Bfa1...
  65. pmc Genetic analysis reveals essential and non-essential amino acids within the telomeric DNA-binding interface of Cdc13p
    Yi Chien Lin
    Institute of Biochemistry and Molecular Biology, National Yang Ming University, Shih Pai 112, Taipei, Taiwan, People s Republic of China
    Biochem J 403:289-95. 2007
    ..A series of mutants within the telomere-binding domain of Cdc13p were identified that failed to complement cdc13 mutants in vivo...
  66. pmc Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s)
    A Yamamoto
    Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    J Cell Biol 133:99-110. 1996
    ..of Cdc20p, another protein implicated in the degradation of mitotic cyclins; and (c) the inactivation of Cdc13 protein or gamma irradiation, two circumstances that induce a DNA-damage checkpoint...
  67. pmc Mutant telomere sequences lead to impaired chromosome separation and a unique checkpoint response
    Jue Lin
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94143 2200, USA
    Mol Biol Cell 15:1623-34. 2004
    ..These results suggest that mutant telomere sequences elicit a checkpoint that is genetically distinct from those activated by deletion of telomerase or DNA damage...
  68. ncbi Budding yeast 14-3-3 proteins contribute to the robustness of the DNA damage and spindle checkpoints
    Nathalie Grandin
    IFR128 BioSciences Gerland, UMR CNRS ENS n degrees 5239, Ecole Normale Superieure de Lyon, Lyon, France
    Cell Cycle 7:2749-61. 2008
    ..These defects were additive with those conferred by the bub2 and mad2 spindle checkpoint mutations. Following cdc13-1-induced DNA damage, the 14-3-3 response was additive with those provided by the Mec1 (ATR-related)-controlled ..
  69. doi Hypermethylation of yeast telomerase RNA by the snRNA and snoRNA methyltransferase Tgs1
    Jacqueline Franke
    Zentrum für Medizinische Biotechnologie, Universitat Duisburg Essen, Universitätsstr 5, 45117 Essen, Germany
    J Cell Sci 121:3553-60. 2008
    ..Furthermore, tgs1Delta cells displayed a shortened replicative lifespan, suggesting that the loss of the m(3)G cap of TLC1 causes premature aging...
  70. ncbi The product of Saccharomyces cerevisiae WHIP/MGS1, a gene related to replication factor C genes, interacts functionally with DNA polymerase delta
    D Branzei
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, 980 8578, Japan
    Mol Genet Genomics 268:371-86. 2002
    ..Possible roles of Mgs1, DNA polymerase delta, Rad18 and Mms2 in replication and replication fork restart are discussed...
  71. ncbi Exposure of single-stranded telomeric DNA causes G2/M cell cycle arrest in Saccharomyces cerevisiae
    Te Ling Pang
    Institutes of Biopharmaceutical Science and Biochemistry, National Yang Ming University, Taipei 112, Taiwan, Republic of China
    J Biol Chem 278:9318-21. 2003
    ..A mutant allele of CDC13, cdc13-1, causes accumulation of single-stranded TG(1-3) DNA near telomeres along with a G(2)/M cell cycle arrest ..
  72. ncbi Degradation-mediated protein quality control in the nucleus
    Richard G Gardner
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Cell 120:803-15. 2005
    ..We propose that San1p-mediated degradation acts as the last line of proteolytic defense against the deleterious accumulation of aberrant proteins in the nucleus and that analogous systems exist in other eukaryotes...
  73. pmc The Rad51 pathway of telomerase-independent maintenance of telomeres can amplify TG1-3 sequences in yku and cdc13 mutants of Saccharomyces cerevisiae
    Nathalie Grandin
    Ecole Normale Superieure de Lyon, UMR CNRS 5665, 69364 Lyon, France
    Mol Cell Biol 23:3721-34. 2003
    In the yeast Saccharomyces cerevisiae, Cdc13, Yku, and telomerase define three parallel pathways for telomere end protection that prevent chromosome instability and death by senescence...
  74. ncbi Characterization of the BUD31 gene of Saccharomyces cerevisiae
    Barbara Masciadri
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    Biochem Biophys Res Commun 320:1342-50. 2004
    ..We propose that the observed phenotypes for bud31-null strain could be the result of defective splicing and indicate a first functional role for Bud3lp and its homologs...
  75. ncbi Rad24 truncation, coupled with altered telomere structure, promotes cdc13-1 suppression in S. cerevisiae
    Vanessa Y Small
    Department of Cell Biology and Neuroscience, University of California, Riverside, Riverside, California 92521, USA
    Cell Cycle 7:3428-39. 2008
    Distinguishing telomeres from DNA double strand breaks is critical for genome stability. In S. cerevisiae, the Cdc13 single-strand telomere binding protein is critical for protecting chromosome ends...
  76. pmc Regulation of the mitotic exit protein kinases Cdc15 and Dbf2
    R Visintin
    Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17 233, 40 Ames Street, Cambridge MA 02139, USA
    Mol Biol Cell 12:2961-74. 2001
    ..BUB2 negatively regulates all 3 events. Loading of Cdc15 on SPBs depends on TEM1, whereas loading of Dbf2 on SPBs and activation of Dbf2 kinase depend on TEM1 and CDC15...
  77. pmc A RAD9-dependent checkpoint blocks meiosis of cdc13 yeast cells
    L Weber
    Department of Genetics, University of Washington, Seattle 98195
    Genetics 131:55-63. 1992
    Mutations in CDC13 have previously been found to cause cell cycle arrest of Saccharomyces cerevisiae at a stage in G2 immediately preceding the mitotic division...
  78. pmc The coordination of centromere replication, spindle formation, and kinetochore-microtubule interaction in budding yeast
    Hong Liu
    Department of Biological Sciences, Florida State University, Tallahassee, Florida, United States of America
    PLoS Genet 4:e1000262. 2008
    ..Therefore, the shorter spindle in S-phase cells is likely to facilitate proper chromosome-microtubule interaction...
  79. pmc A genome wide analysis of the response to uncapped telomeres in budding yeast reveals a novel role for the NAD+ biosynthetic gene BNA2 in chromosome end protection
    Amanda Greenall
    Aging Research Laboratories, Institute for Aging and Health, Newcastle University, Newcastle upon Tyne, UK
    Genome Biol 9:R146. 2008
    ..In order to better understand the eukaryotic response to telomere damage, budding yeast strains harboring a temperature sensitive allele of an essential telomere capping gene (cdc13-1) were subjected to a transcriptomic study.
  80. doi Ten1p promotes the telomeric DNA-binding activity of Cdc13p: implication for its function in telomere length regulation
    Wei Qian
    The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, The Graduate School of Chinese Academy of Sciences, Shanghai, China
    Cell Res 19:849-63. 2009
    In Saccharomyces cerevisiae, the essential gene CDC13 encodes a telomeric single-stranded DNA-binding protein that interacts with Stn1p and Ten1p genetically and physically, and is required for telomere end protection and telomere length ..
  81. pmc Telomere-binding and Stn1p-interacting activities are required for the essential function of Saccharomyces cerevisiae Cdc13p
    M J Wang
    Institute of Biopharmaceutical Science, National Yang Ming University, Shih Pai, 112, Taipei, Taiwan, Republic of China
    Nucleic Acids Res 28:4733-41. 2000
    ..the telomere-binding region of Cdc13p (amino acids 451-693) was not capable of complementing the growth defects of cdc13 mutants...
  82. pmc Inactivation of the Sas2 histone acetyltransferase delays senescence driven by telomere dysfunction
    Marina L Kozak
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6100, USA
    EMBO J 29:158-70. 2010
    ..In contrast, sir4 deletion sped senescence and blocked the delay conferred by sas2 or sir3 deletion. We thus show that manipulation of telomere chromatin modulates senescence caused by telomere shortening...
  83. pmc Rapid Cdc13 turnover and telomere length homeostasis are controlled by Cdk1-mediated phosphorylation of Cdc13
    Shun Fu Tseng
    Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan
    Nucleic Acids Res 37:3602-11. 2009
    Budding yeast telomerase is mainly activated by Tel1/Mec1 (yeast ATM/ATR) on Cdc13 from late S to G2 phase of the cell cycle...
  84. pmc Inhibition of yeast telomerase action by the telomeric ssDNA-binding protein, Cdc13p
    David C Zappulla
    Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309, USA
    Nucleic Acids Res 37:354-67. 2009
    ....
  85. ncbi STM1, a gene which encodes a guanine quadruplex binding protein, interacts with CDC13 in Saccharomyces cerevisiae
    N Hayashi
    Department of Molecular Oncology, Cancer Research Institute, Kanazawa University, 13 1 Takara machi, Kanazawa, Ishikawa 920 0934, Japan
    Mol Genet Genomics 267:806-13. 2002
    The CDC13 gene encodes a protein that binds to the G-rich single-strand at yeast telomeres, and serves as a regulator of telomere replication...
  86. pmc Dynamic regulation of single-stranded telomeres in Saccharomyces cerevisiae
    Stephanie Smith
    Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genetics 178:693-701. 2008
    ....
  87. ncbi Cdc20, a beta-transducin homologue, links RAD9-mediated G2/M checkpoint control to mitosis in Saccharomyces cerevisiae
    H H Lim
    Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Mol Gen Genet 253:138-48. 1996
    ..These genes also prevent entry into mitosis of a temperature-sensitive mutant, cdc13, that accumulates chromosome damage at 37 degrees C...