CDC10

Summary

Gene Symbol: CDC10
Description: septin CDC10
Alias: septin CDC10
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Meitinger F, Boehm M, Hofmann A, Hub B, Zentgraf H, Lehmann W, et al. Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis. Genes Dev. 2011;25:875-88 pubmed publisher
    ..Genetic and functional data support a model in which Dbf2-Mob1 regulates Hof1 by inducing domain rearrangements, leading to the exposure of the Hof1 RLS domain during telophase. ..
  2. Demarini D, Adams A, Fares H, De Virgilio C, Valle G, Chuang J, et al. A septin-based hierarchy of proteins required for localized deposition of chitin in the Saccharomyces cerevisiae cell wall. J Cell Biol. 1997;139:75-93 pubmed
    ..Taken together, these results suggest that the normal localization of chitin synthase III activity is achieved by assembly of a complex in which Chs3p is linked to the septins via Chs4p and Bni4p. ..
  3. Caviston J, Longtine M, Pringle J, Bi E. The role of Cdc42p GTPase-activating proteins in assembly of the septin ring in yeast. Mol Biol Cell. 2003;14:4051-66 pubmed
  4. Casamayor A, Snyder M. Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function. Mol Cell Biol. 2003;23:2762-77 pubmed
    ..Thus, we find that septins are multifunctional proteins with specific domains involved in distinct molecular interactions required for assembly, localization, and function within the cell. ..
  5. Bertin A, McMurray M, Grob P, Park S, Garcia G, Patanwala I, et al. Saccharomyces cerevisiae septins: supramolecular organization of heterooligomers and the mechanism of filament assembly. Proc Natl Acad Sci U S A. 2008;105:8274-9 pubmed publisher
    Mitotic yeast cells express five septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1/Sep7). Only Shs1 is nonessential. The four essential septins form a complex containing two copies of each, but their arrangement was not known...
  6. Pruyne D, Gao L, Bi E, Bretscher A. Stable and dynamic axes of polarity use distinct formin isoforms in budding yeast. Mol Biol Cell. 2004;15:4971-89 pubmed
    ..By coupling different formin isoforms to unique cortical landmarks, yeast uses common cytoskeletal elements to maintain stable and dynamic axes in the same cell. ..
  7. Dobbelaere J, Gentry M, Hallberg R, Barral Y. Phosphorylation-dependent regulation of septin dynamics during the cell cycle. Dev Cell. 2003;4:345-57 pubmed
    ..Together our results implicate septins in a late step of cytokinesis and indicate that proper regulation of septin dynamics, possibly through the control of their phosphorylation state, is required for the completion of cytokinesis. ..
  8. Cid V, Adamíková L, Sanchez M, Molina M, Nombela C. Cell cycle control of septin ring dynamics in the budding yeast. Microbiology. 2001;147:1437-50 pubmed
    Septins constitute a cytoskeletal structure that is conserved in eukaryotes. In Saccharomyces cerevisiae, the Cdc3, Cdc10, Cdc11, Cdc12 and Shs1/Sep7 septins assemble as a ring that marks the cytokinetic plane throughout the budding ..
  9. Kozubowski L, Larson J, Tatchell K. Role of the septin ring in the asymmetric localization of proteins at the mother-bud neck in Saccharomyces cerevisiae. Mol Biol Cell. 2005;16:3455-66 pubmed
    ..These observations provide clues to the mechanisms for the asymmetric localization of septin-associated proteins. ..

More Information

Publications57

  1. Versele M, Gullbrand B, Shulewitz M, Cid V, Bahmanyar S, Chen R, et al. Protein-protein interactions governing septin heteropentamer assembly and septin filament organization in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:4568-83 pubmed
    Mitotic yeast (Saccharomyces cerevisiae) cells express five related septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1) that form a cortical filamentous collar at the mother-bud neck necessary for normal morphogenesis and cytokinesis...
  2. Flescher E, Madden K, Snyder M. Components required for cytokinesis are important for bud site selection in yeast. J Cell Biol. 1993;122:373-86 pubmed
    ..In this report, a genetic screen was used to isolate a novel ochre allele of CDC10, cdc10-10; strains containing this mutation require the SPA2 gene for growth...
  3. Iwase M, Luo J, Bi E, Toh e A. Shs1 plays separable roles in septin organization and cytokinesis in Saccharomyces cerevisiae. Genetics. 2007;177:215-29 pubmed
    In Saccharomyces cerevisiae, five septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1/Sep7) form the septin ring at the bud neck during vegetative growth...
  4. Versele M, Thorner J. Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4. J Cell Biol. 2004;164:701-15 pubmed
    Assembly at the mother-bud neck of a filamentous collar containing five septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1) is necessary for proper morphogenesis and cytokinesis...
  5. Nagaraj S, Rajendran A, Jackson C, Longtine M. Role of nucleotide binding in septin-septin interactions and septin localization in Saccharomyces cerevisiae. Mol Cell Biol. 2008;28:5120-37 pubmed publisher
    ..We find no evidence that nucleotide binding is specifically involved in the interaction of septins with septin-associated proteins. ..
  6. McMurray M, Thorner J. Septin stability and recycling during dynamic structural transitions in cell division and development. Curr Biol. 2008;18:1203-8 pubmed publisher
    ..that are incorporated into distinct structures during cell division and differentiation; yeast septins Cdc3, Cdc10, Cdc11, and Cdc12 form hetero-octamers and polymerize into filaments, which form a "collar" at the ..
  7. Cid V, Adamíková L, Cenamor R, Molina M, Sanchez M, Nombela C. Cell integrity and morphogenesis in a budding yeast septin mutant. Microbiology. 1998;144 ( Pt 12):3463-74 pubmed
    ..The septin-encoding CDC10 gene was cloned by complementation of the pleiotropic phenotype of the V327 mutant...
  8. Mortensen E, McDonald H, Yates J, Kellogg D. Cell cycle-dependent assembly of a Gin4-septin complex. Mol Biol Cell. 2002;13:2091-105 pubmed
    ..tight complex, and we found that the majority of the Shs1 in the cell is tightly bound to the other septins Cdc3, Cdc10, Cdc11, and Cdc12...
  9. Garcia G, Bertin A, Li Z, Song Y, McMurray M, Thorner J, et al. Subunit-dependent modulation of septin assembly: budding yeast septin Shs1 promotes ring and gauze formation. J Cell Biol. 2011;195:993-1004 pubmed publisher
    ..In budding yeast, five mitotic septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1), which are essential for cytokinesis, transition during bud growth from a patch to a ..
  10. Carroll C, Altman R, Schieltz D, Yates J, Kellogg D. The septins are required for the mitosis-specific activation of the Gin4 kinase. J Cell Biol. 1998;143:709-17 pubmed
    ..These results demonstrate that the septins in budding yeast play an integral role in the mitosis-specific regulation of the Gin4 kinase and that they carry out functions early in mitosis. ..
  11. Barral Y, Mermall V, Mooseker M, Snyder M. Compartmentalization of the cell cortex by septins is required for maintenance of cell polarity in yeast. Mol Cell. 2000;5:841-51 pubmed
    ..We propose that septins maintain cell polarity by specifying a boundary between cortical domains. ..
  12. Vrabioiu A, Gerber S, Gygi S, Field C, Mitchison T. The majority of the Saccharomyces cerevisiae septin complexes do not exchange guanine nucleotides. J Biol Chem. 2004;279:3111-8 pubmed
    ..We conclude that bound GTP and GDP play a structural, rather then regulatory, role for the majority of septins in proliferating cells as GTP does for alpha-tubulin. ..
  13. Lee P, Song S, Ro H, Park C, Lippincott J, Li R, et al. Bni5p, a septin-interacting protein, is required for normal septin function and cytokinesis in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:6906-20 pubmed
    ..Overexpression of BNI5 also suppressed the growth defects of cdc10-1, cdc11-6, and sep7Delta strains...
  14. Wloka C, Nishihama R, Onishi M, Oh Y, Hanna J, Pringle J, et al. Evidence that a septin diffusion barrier is dispensable for cytokinesis in budding yeast. Biol Chem. 2011;392:813-29 pubmed publisher
    ..Here, we show that in cells lacking the septin Cdc10 or the septin-associated protein Bud4, the septins form a ring-like structure at the mother-bud neck that fails ..
  15. Farkasovsky M, Herter P, Voss B, Wittinghofer A. Nucleotide binding and filament assembly of recombinant yeast septin complexes. Biol Chem. 2005;386:643-56 pubmed
    ..In the yeast Saccharomyces cerevisiae, the septins encoded by CDC3, CDC10, CDC11, and CDC12 form a high-molecular-weight complex, localized at the cytoplasmic face of the plasma membrane ..
  16. Hartwell L. Genetic control of the cell division cycle in yeast. IV. Genes controlling bud emergence and cytokinesis. Exp Cell Res. 1971;69:265-76 pubmed
  17. Egelhofer T, Villen J, McCusker D, Gygi S, Kellogg D. The septins function in G1 pathways that influence the pattern of cell growth in budding yeast. PLoS ONE. 2008;3:e2022 pubmed publisher
    ..Phosphorylation of Shs1 by G1 cyclin-dependent kinases plays a role in events that influence Cdk1 inhibitory phosphorylation...
  18. Longtine M, Demarini D, Valencik M, Al Awar O, Fares H, De Virgilio C, et al. The septins: roles in cytokinesis and other processes. Curr Opin Cell Biol. 1996;8:106-19 pubmed
    ..The septins also appear to be involved in various other aspects of the organization of the cell surface. ..
  19. Iwase M, Toh e A. Nis1 encoded by YNL078W: a new neck protein of Saccharomyces cerevisiae. Genes Genet Syst. 2001;76:335-43 pubmed
    ..GFP-Nis1 is localized in the nucleus throughout the cell cycle and in the bud neck at G2/M phase in a septin-dependent manner. Altogether, the findings suggest that Nis1 may play a non-essential role in the mitotic signaling network. ..
  20. McMurray M, Stefan C, Wemmer M, Odorizzi G, Emr S, Thorner J. Genetic interactions with mutations affecting septin assembly reveal ESCRT functions in budding yeast cytokinesis. Biol Chem. 2011;392:699-712 pubmed publisher
    ..cell division, and alleviate phenotypes of cells carrying temperature-sensitive alleles of a septin-encoding gene, CDC10. Elevated chitin synthase II (Chs2) levels coupled with aberrant morphogenesis and chitin deposition in elm1? ..
  21. Frazier J, Wong M, Longtine M, Pringle J, Mann M, Mitchison T, et al. Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function. J Cell Biol. 1998;143:737-49 pubmed
    ..relationship between these filaments and the neck filaments, we purified septin complexes from cells deleted for CDC10 or CDC11...
  22. Horowitz A, Lapointe J, Eid R, Sheibani S, Gharib N, Jones N, et al. The human septin7 and the yeast CDC10 septin prevent Bax and copper mediated cell death in yeast. Biochim Biophys Acta. 2013;1833:3186-3194 pubmed publisher
    ..Overexpression of the yeast septin7 ortholog CDC10 also conferred resistance to the negative effects of copper as well as protecting cells from the overexpression of ..
  23. Perez J, Arcones I, Gomez A, Casquero V, Roncero C. Phosphorylation of Bni4 by MAP kinases contributes to septum assembly during yeast cytokinesis. FEMS Yeast Res. 2016;16: pubmed publisher
    ..Using an assay based on hypersensitive cells of the cdc10-11 mutant, we have pinpointed several MAP kinase phosphorylatable residues required for Bni4 function...
  24. Johnson C, Weems A, Brewer J, Thorner J, McMurray M. Cytosolic chaperones mediate quality control of higher-order septin assembly in budding yeast. Mol Biol Cell. 2015;26:1323-44 pubmed publisher
    ..These findings provide new roles for chaperone-mediated kinetic partitioning of non-native proteins and may help explain the etiology of septin-linked human diseases. ..
  25. Heasley L, McMurray M. Roles of septins in prospore membrane morphogenesis and spore wall assembly in Saccharomyces cerevisiae. Mol Biol Cell. 2016;27:442-50 pubmed publisher
    ..Strikingly, extra copies of septin CDC10 rescue sporulation and LEP localization in cells lacking Sma1, a phospholipase D-associated protein dispensable ..
  26. Okuzaki D, Nojima H. Kcc4 associates with septin proteins of Saccharomyces cerevisiae. FEBS Lett. 2001;489:197-201 pubmed
    ..From the results obtained here, we suggest that Cdc11 is the primary association partner of Kcc4. ..
  27. Pablo Hernando M, Arnaiz Pita Y, Tachikawa H, del Rey F, Neiman A, Vazquez de Aldana C. Septins localize to microtubules during nutritional limitation in Saccharomyces cerevisiae. BMC Cell Biol. 2008;9:55 pubmed publisher
    ..we demonstrate that nutrient limitation triggers a change in the localization of at least two vegetative septins (Cdc10 and Cdc11) from the bud neck to the microtubules...
  28. Weems A, Johnson C, Argueso J, McMurray M. Higher-order septin assembly is driven by GTP-promoted conformational changes: evidence from unbiased mutational analysis in Saccharomyces cerevisiae. Genetics. 2014;196:711-27 pubmed publisher
    ..identify a mutation in septin Cdc3 that restores the high-temperature assembly competence of a mutant allele of septin Cdc10, likely by adopting a conformation more compatible with nucleotide-free Cdc10...
  29. Qiu W, Neo S, Yu X, Cai M. A novel septin-associated protein, Syp1p, is required for normal cell cycle-dependent septin cytoskeleton dynamics in yeast. Genetics. 2008;180:1445-57 pubmed publisher
    ..These results suggest that Syp1p is involved in the regulation of cell cycle-dependent dynamics of the septin cytoskeleton in yeast. ..
  30. Finnigan G, Booth E, Duvalyan A, Liao E, Thorner J. The Carboxy-Terminal Tails of Septins Cdc11 and Shs1 Recruit Myosin-II Binding Factor Bni5 to the Bud Neck in Saccharomyces cerevisiae. Genetics. 2015;200:843-62 pubmed publisher
    ..Related article in Finnigan, G. C. et al., 2015 Comprehensive Genetic Analysis of Paralogous Terminal Septin Subunits Shs1 and Cdc11 in Saccharomyces cerevisiae. Genetics 200: 841-861. ..
  31. Sadian Y, Gatsogiannis C, Patasi C, Hofnagel O, Goody R, Farkasovsk M, et al. The role of Cdc42 and Gic1 in the regulation of septin filament formation and dissociation. elife. 2013;2:e01085 pubmed publisher
    ..Based on our results we propose a novel regulatory mechanism for septin filament formation and dissociation. DOI: http://dx.doi.org/10.7554/eLife.01085.001...
  32. Moreau V, Madania A, Martin R, Winson B. The Saccharomyces cerevisiae actin-related protein Arp2 is involved in the actin cytoskeleton. J Cell Biol. 1996;134:117-32 pubmed
    ..In addition, genetic interaction was observed between temperature-sensitive alleles arp2-H330L and cdc10-1...
  33. Orii M, Kono K, Wen H, Nakanishi M. PP1-Dependent Formin Bnr1 Dephosphorylation and Delocalization from a Cell Division Site. PLoS ONE. 2016;11:e0146941 pubmed publisher
    ..Further, we identified Ref2 as the PP1 regulatory subunit responsible for this regulation. Taken together, Glc7 and Ref2 may have a role in actomyosin ring formation by modulating the localization of formins during cytokinesis. ..
  34. Sung H, Chul Han K, Chul Kim J, Wan Oh K, Su Yoo H, Tae Hong J, et al. A set of epitope-tagging integration vectors for functional analysis in Saccharomyces cerevisiae. FEMS Yeast Res. 2005;5:943-50 pubmed
    ..a component of the septin filament that encircles the mother bud neck and consists of five major proteins: Cdc3, Cdc10, Cdc11, Cdc12, and Sep7...
  35. Oh Y, Schreiter J, Nishihama R, Wloka C, Bi E. Targeting and functional mechanisms of the cytokinesis-related F-BAR protein Hof1 during the cell cycle. Mol Biol Cell. 2013;24:1305-20 pubmed publisher
    ..is mediated mainly by an interaction between the second coiled-coil region in the N-terminus and the septin Cdc10, whereas the localization to the sites of polarized growth is mediated entirely by the F-BAR domain...
  36. James A, Cocheme H, Murai M, Miyoshi H, Murphy M. Complementation of coenzyme Q-deficient yeast by coenzyme Q analogues requires the isoprenoid side chain. FEBS J. 2010;277:2067-82 pubmed publisher
    ..Here we suggest that CoQ or its redox state may be a signal for growth during the shift to respiration. ..
  37. Meitinger F, Palani S, Hub B, Pereira G. Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis. Mol Biol Cell. 2013;24:1290-304 pubmed publisher
    ..Thus our data show that Dbf2 coordinates septin and AMR functions during cytokinesis through the regulation/control of Hof1. ..
  38. Weems A, MCMURRAY M. The step-wise pathway of septin hetero-octamer assembly in budding yeast. elife. 2017;6: pubmed publisher
    ..stepwise assembly pathway produces the two species of budding yeast septin hetero-octamers: Cdc11/Shs1-Cdc12-Cdc3-Cdc10-Cdc10-Cdc3-Cdc12-Cdc11/Shs1. Rapid GTP hydrolysis by monomeric Cdc10 drives assembly of the core Cdc10 homodimer...
  39. Renz C, Johnsson N, Gronemeyer T. An efficient protocol for the purification and labeling of entire yeast septin rods from E.coli for quantitative in vitro experimentation. BMC Biotechnol. 2013;13:60 pubmed publisher
    ..In yeast, they comprise a family of proteins (Cdc3, Cdc10, Cdc11, Cdc12, Shs1) that form a co-polymeric, ring-like structure beneath the membrane...
  40. Garcia G, Finnigan G, Heasley L, Sterling S, Aggarwal A, Pearson C, et al. Assembly, molecular organization, and membrane-binding properties of development-specific septins. J Cell Biol. 2016;212:515-29 pubmed publisher
    ..In vitro, the sole stable complex that contains both meiosis-specific septins is a linear Spr28-Spr3-Cdc3-Cdc10-Cdc10-Cdc3-Spr3-Spr28 hetero-octamer...
  41. Castillon G, Adames N, Rosello C, Seidel H, Longtine M, Cooper J, et al. Septins have a dual role in controlling mitotic exit in budding yeast. Curr Biol. 2003;13:654-8 pubmed
    ..These results suggest that septins serve as a diffusion barrier for Lte1p, and that Cdc10p is needed for the septin ring to serve as a scaffold for a putative microtubule sensor. ..
  42. Finnigan G, Takagi J, Cho C, Thorner J. Comprehensive Genetic Analysis of Paralogous Terminal Septin Subunits Shs1 and Cdc11 in Saccharomyces cerevisiae. Genetics. 2015;200:821-41 pubmed publisher
    ..Shs1, and the basic building blocks for assembling septin structures are hetero-octameric rods (Cdc11-Cdc12-Cdc3-Cdc10-Cdc10-Cdc3-Cdc12-Cdc11 and Shs1-Cdc12-Cdc3-Cdc10-Cdc10-Cdc3-Cdc12-Shs1)...
  43. Bharucha J, Larson J, Konopka J, Tatchell K. Saccharomyces cerevisiae Afr1 protein is a protein phosphatase 1/Glc7-targeting subunit that regulates the septin cytoskeleton during mating. Eukaryot Cell. 2008;7:1246-55 pubmed publisher
    ..These results demonstrate a previously unknown role for Afr1 in targeting Glc7 to mating projections and in regulating the septin architecture during mating. ..
  44. de Val N, McMurray M, Lam L, Hsiung C, Bertin A, Nogales E, et al. Native cysteine residues are dispensable for the structure and function of all five yeast mitotic septins. Proteins. 2013;81:1964-79 pubmed publisher
    ..locations, site-directed mutagenesis was used, first, to replace the native Cys residues in Cdc3 (C124 C253 C279), Cdc10 (C266), Cdc11 (C43 C137 C138), Cdc12 (C40 C278), and Shs1 (C29 C148) with Ala, Ser, Val, or Phe...
  45. Booth E, Vane E, Dovala D, Thorner J. A Förster Resonance Energy Transfer (FRET)-based System Provides Insight into the Ordered Assembly of Yeast Septin Hetero-octamers. J Biol Chem. 2015;290:28388-401 pubmed publisher
    ..In mitotically growing yeast cells, five septin subunits are expressed (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1) and assemble into two types of rods that differ only in their terminal subunit: Cdc11-..
  46. Madden K, Snyder M. Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol. 1998;52:687-744 pubmed
    ..Since many of the components important for polarized cell growth are conserved in other organisms, the basic mechanisms mediating polarized cell growth are likely to be universal among eukaryotes. ..
  47. Roemer T, Madden K, Chang J, Snyder M. Selection of axial growth sites in yeast requires Axl2p, a novel plasma membrane glycoprotein. Genes Dev. 1996;10:777-93 pubmed
    Spa2p and Cdc10p both participate in bud site selection and cell morphogenesis in yeast, and spa2delta cdc10-10 cells are inviable...
  48. Kim J, Rose M. Stable Pseudohyphal Growth in Budding Yeast Induced by Synergism between Septin Defects and Altered MAP-kinase Signaling. PLoS Genet. 2015;11:e1005684 pubmed publisher
    ..Taken together, our findings show that budding yeast can access a stable constitutive pseudohyphal growth state with very few genetic and regulatory changes. ..