Gene Symbol: CCT6
Description: chaperonin-containing T-complex subunit CCT6
Alias: HTR3, TCP20, TCP6, chaperonin-containing T-complex subunit CCT6
Species: Saccharomyces cerevisiae S288c
Dunn C, Jensen R. Suppression of a defect in mitochondrial protein import identifies cytosolic proteins required for viability of yeast cells lacking mitochondrial DNA. Genetics. 2003;165:35-45 pubmed
..We identified several genes encoding cytosolic proteins, including CCT6, SSB1, ICY1, TIP41, and PBP1, which, when overproduced, rescue the mtDNA dependence of tim18Delta cells...
Schmidt A, Kunz J, Hall M. TOR2 is required for organization of the actin cytoskeleton in yeast. Proc Natl Acad Sci U S A. 1996;93:13780-5 pubmed
..A screen for dosage suppressors of a dominant negative TOR2 allele identified TCP20/CCT6, encoding a subunit of the cytosolic chaperonin TCP-1 that is involved in the biogenesis of actin structures...
Lin P, Sherman F. The unique hetero-oligomeric nature of the subunits in the catalytic cooperativity of the yeast Cct chaperonin complex. Proc Natl Acad Sci U S A. 1997;94:10780-5 pubmed
..We demonstrated herein that cct6-3 (L19S), but not the parolog cct1-5 (R26I), specifically suppresses the cct1-1, cct2-3, and cct3-1 alleles, and ..
Pappenberger G, McCormack E, Willison K. Quantitative actin folding reactions using yeast CCT purified via an internal tag in the CCT3/gamma subunit. J Mol Biol. 2006;360:484-96 pubmed
..In this pure in vitro system, the human beta-actin mutants, D244S and G150P, show impaired folding behaviour in the manner predicted by our sequence-specific recognition model for CCT-actin interaction. ..
Hwang D, Claypool S, Leuenberger D, Tienson H, Koehler C. Tim54p connects inner membrane assembly and proteolytic pathways in the mitochondrion. J Cell Biol. 2007;178:1161-75 pubmed
..As such, Tim54p links protein import, assembly, and turnover pathways in the mitochondrion. ..
Amit M, Weisberg S, Nadler Holly M, McCormack E, Feldmesser E, Kaganovich D, et al
. Equivalent mutations in the eight subunits of the chaperonin CCT produce dramatically different cellular and gene expression phenotypes. J Mol Biol. 2010;401:532-43 pubmed publisher
..System-level analysis of the strains using RNA microarrays reveals connections between CCT and several cellular networks, including ribosome biogenesis and TOR2, that help to explain the phenotypic variability observed. ..
Kabir M, Kaminska J, Segel G, Bethlendy G, Lin P, Della Seta F, et al
. Physiological effects of unassembled chaperonin Cct subunits in the yeast Saccharomyces cerevisiae. Yeast. 2005;22:219-39 pubmed
..Nevertheless, overexpression of certain Cct subunits, especially CCT6, suppresses a wide range of abnormal phenotypes, including those caused by the diverse types of conditional ..
Shevchenko A, Schaft D, Roguev A, Pijnappel W, Stewart A, Shevchenko A. Deciphering protein complexes and protein interaction networks by tandem affinity purification and mass spectrometry: analytical perspective. Mol Cell Proteomics. 2002;1:204-12 pubmed
..Concordance with the results of genome-wide two-hybrid screening was poor (14% of identified interactors overlapped) suggesting that the two approaches may provide complementary views on physical interactions within the proteome. ..
Zang Y, Jin M, Wang H, Cui Z, Kong L, Liu C, et al
. Staggered ATP binding mechanism of eukaryotic chaperonin TRiC (CCT) revealed through high-resolution cryo-EM. Nat Struct Mol Biol. 2016;23:1083-1091 pubmed publisher
..structural and biochemical data reveal a staggered ATP binding mechanism of TRiC with preloaded nucleotide on the CCT6 side of NPP-TRiC and demonstrate that TRiC has evolved into a complex that is structurally divided into two sides...