CCR4

Summary

Gene Symbol: CCR4
Description: CCR4-NOT core exoribonuclease subunit CCR4
Alias: FUN27, NUT21, CCR4-NOT core exoribonuclease subunit CCR4
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Hata H, Mitsui H, Liu H, Bai Y, Denis C, Shimizu Y, et al. Dhh1p, a putative RNA helicase, associates with the general transcription factors Pop2p and Ccr4p from Saccharomyces cerevisiae. Genetics. 1998;148:571-9 pubmed
    ..We have isolated five multicopy suppressor genes of a pop2 deletion mutation: CCR4, DHH1 (a putative RNA helicase), PKC1, STM1, and MPT5 (multicopy suppressor of pop two)...
  2. Deluen C, James N, Maillet L, Molinete M, Theiler G, Lemaire M, et al. The Ccr4-not complex and yTAF1 (yTaf(II)130p/yTaf(II)145p) show physical and functional interactions. Mol Cell Biol. 2002;22:6735-49 pubmed
    The Saccharomyces cerevisiae Ccr4-Not complex is a global regulator of transcription that is thought to regulate TATA binding protein (TBP) function at certain promoters specifically...
  3. Manukyan A, Zhang J, Thippeswamy U, Yang J, Zavala N, Mudannayake M, et al. Ccr4 alters cell size in yeast by modulating the timing of CLN1 and CLN2 expression. Genetics. 2008;179:345-57 pubmed publisher
    Large, multisubunit Ccr4-Not complexes are evolutionarily conserved global regulators of gene expression. Deletion of CCR4 or several components of Ccr4-Not complexes results in abnormally large cells...
  4. Tucker M, Staples R, Valencia Sanchez M, Muhlrad D, Parker R. Ccr4p is the catalytic subunit of a Ccr4p/Pop2p/Notp mRNA deadenylase complex in Saccharomyces cerevisiae. EMBO J. 2002;21:1427-36 pubmed
    ..We now demonstrate that CCR4 encodes the catalytic subunit of the deadenylase and that Pop2p is dispensable for catalysis...
  5. Liu H, Badarinarayana V, Audino D, Rappsilber J, Mann M, Denis C. The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively. EMBO J. 1998;17:1096-106 pubmed
    The CCR4 transcriptional regulatory complex consisting of CCR4, CAF1, DBF2 and other unidentified factors is one of several groups of proteins that affect gene expression...
  6. Chang M, French Cornay D, Fan H, Klein H, Denis C, Jaehning J. A complex containing RNA polymerase II, Paf1p, Cdc73p, Hpr1p, and Ccr4p plays a role in protein kinase C signaling. Mol Cell Biol. 1999;19:1056-67 pubmed
    ..Our observation that the Mpk1p kinase is fully active in a paf1Delta strain indicates that the Paf1p-Cdc73p complex may function downstream of the Pkc1p-Mpk1p cascade to regulate the expression of a subset of yeast genes. ..
  7. Westmoreland T, Marks J, Olson J, Thompson E, Resnick M, Bennett C. Cell cycle progression in G1 and S phases is CCR4 dependent following ionizing radiation or replication stress in Saccharomyces cerevisiae. Eukaryot Cell. 2004;3:430-46 pubmed
    ..databases, many of these genes were found to interact in a damage response network with the transcription factor Ccr4, a core component of the CCR4-NOT and RNA polymerase-associated factor 1 (PAF1)-CDC73 transcription complexes...
  8. Azzouz N, Panasenko O, Deluen C, Hsieh J, Theiler G, Collart M. Specific roles for the Ccr4-Not complex subunits in expression of the genome. RNA. 2009;15:377-83 pubmed publisher
    In this work we used micro-array experiments to determine the role of each nonessential subunit of the conserved Ccr4-Not complex in the control of gene expression in the yeast Saccharomyces cerevisiae...
  9. Reijns M, Alexander R, Spiller M, Beggs J. A role for Q/N-rich aggregation-prone regions in P-body localization. J Cell Sci. 2008;121:2463-72 pubmed publisher
    ..This is supported by reduced P-body accumulation of Ccr4p, Pop2p and Dhh1p after deletion of these domains, and by the observed aggregation of the Q/N-rich region from Ccr4p. ..

More Information

Publications73

  1. Basquin J, Roudko V, Rode M, Basquin C, Seraphin B, Conti E. Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction. Mol Cell. 2012;48:207-18 pubmed publisher
    ..The major cytoplasmic deadenylase, the Ccr4-Not complex, is a conserved multisubunit assembly...
  2. Daugeron M, Mauxion F, Seraphin B. The yeast POP2 gene encodes a nuclease involved in mRNA deadenylation. Nucleic Acids Res. 2001;29:2448-55 pubmed
    ..Similar phenotypes are observed in yeast strains lacking the Ccr4 protein, which is known to be associated with Pop2p...
  3. Cui Y, Ramnarain D, Chiang Y, Ding L, McMahon J, Denis C. Genome wide expression analysis of the CCR4-NOT complex indicates that it consists of three modules with the NOT module controlling SAGA-responsive genes. Mol Genet Genomics. 2008;279:323-37 pubmed publisher
    Of the nine known members of the CCR4-NOT complex, CCR4/CAF1 are most important in mRNA deadenylation whereas the NOT1-5 proteins are most critical for transcriptional repression...
  4. Lemaire M, Collart M. The TATA-binding protein-associated factor yTafII19p functionally interacts with components of the global transcriptional regulator Ccr4-Not complex and physically interacts with the Not5 subunit. J Biol Chem. 2000;275:26925-34 pubmed
    ..They encode proteins associated in a complex that also contains the Caf1 and Ccr4 proteins...
  5. Azzouz N, Panasenko O, Colau G, Collart M. The CCR4-NOT complex physically and functionally interacts with TRAMP and the nuclear exosome. PLoS ONE. 2009;4:e6760 pubmed publisher
    b>Ccr4-Not is a highly conserved multi-protein complex consisting in yeast of 9 subunits, including Not5 and the major yeast deadenylase Ccr4...
  6. Traven A, Beilharz T, Lo T, Lueder F, Preiss T, Heierhorst J. The Ccr4-Pop2-NOT mRNA deadenylase contributes to septin organization in Saccharomyces cerevisiae. Genetics. 2009;182:955-66 pubmed publisher
    ..Here we establish that the Ccr4-Pop2-NOT mRNA deadenylase contributes to septin organization...
  7. Lenssen E, Azzouz N, Michel A, Landrieux E, Collart M. The Ccr4-not complex regulates Skn7 through Srb10 kinase. Eukaryot Cell. 2007;6:2251-9 pubmed
    The Ccr4-Not complex is a multifunctional regulatory platform composed of nine subunits that controls diverse cellular events including mRNA degradation, protein ubiquitination, and transcription...
  8. Badarinarayana V, Chiang Y, Denis C. Functional interaction of CCR4-NOT proteins with TATAA-binding protein (TBP) and its associated factors in yeast. Genetics. 2000;155:1045-54 pubmed
    The CCR4-NOT transcriptional regulatory complex affects expression of a number of genes both positively and negatively...
  9. Denis C, Chiang Y, Cui Y, Chen J. Genetic evidence supports a role for the yeast CCR4-NOT complex in transcriptional elongation. Genetics. 2001;158:627-34 pubmed
    The CCR4-NOT complex is involved in the regulation of gene expression both positively and negatively...
  10. Liu H, Chiang Y, Pan J, Chen J, Salvadore C, Audino D, et al. Characterization of CAF4 and CAF16 reveals a functional connection between the CCR4-NOT complex and a subset of SRB proteins of the RNA polymerase II holoenzyme. J Biol Chem. 2001;276:7541-8 pubmed
    The CCR4-NOT transcriptional regulatory complex affects transcription both positively and negatively and consists of the following two complexes: a core 1 x 10(6) dalton (1 MDa) complex consisting of CCR4, CAF1, and the five NOT proteins ..
  11. Bai Y, Salvadore C, Chiang Y, Collart M, Liu H, Denis C. The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5. Mol Cell Biol. 1999;19:6642-51 pubmed
    The CCR4-NOT complex (1 mDa in size), consisting of the proteins CCR4, CAF1, and NOT1 to NOT5, regulates gene expression both positively and negatively and is distinct from other large transcriptional complexes in Saccharomyces cerevisiae ..
  12. Mulder K, Brenkman A, Inagaki A, van den Broek N, Timmers H. Regulation of histone H3K4 tri-methylation and PAF complex recruitment by the Ccr4-Not complex. Nucleic Acids Res. 2007;35:2428-39 pubmed
    ..We found that the evolutionarily conserved Ccr4-Not complex is involved in establishing H3K4 tri-methylation in Saccharomyces cerevisiae...
  13. Traven A, Hammet A, Tenis N, Denis C, Heierhorst J. Ccr4-not complex mRNA deadenylase activity contributes to DNA damage responses in Saccharomyces cerevisiae. Genetics. 2005;169:65-75 pubmed
    ..Some components of the yeast Ccr4-Not complex, which regulates transcription as well as transcript turnover, have previously been linked to DNA ..
  14. Goldstrohm A, Seay D, Hook B, Wickens M. PUF protein-mediated deadenylation is catalyzed by Ccr4p. J Biol Chem. 2007;282:109-14 pubmed
    ..Our data suggest that PUF proteins may enhance mRNA degradation and repress expression by both deadenylation-dependent and -independent mechanisms, using the same Pop2p bridge to recruit a multifunctional Pop2p complex to the mRNA. ..
  15. Laribee R, Shibata Y, Mersman D, Collins S, Kemmeren P, Roguev A, et al. CCR4/NOT complex associates with the proteasome and regulates histone methylation. Proc Natl Acad Sci U S A. 2007;104:5836-41 pubmed
    ..of the Set1 complex that mediate histone H3 lysine 4 methylation (H3K4me), we found that deleting members of the CCR4/NOT mRNA processing complex exhibit synthetic phenotypes when combined with proteasome mutants...
  16. Kaeberlein M, Guarente L. Saccharomyces cerevisiae MPT5 and SSD1 function in parallel pathways to promote cell wall integrity. Genetics. 2002;160:83-95 pubmed
    ..This work also provides evidence that post-transcriptional regulation is likely to be important both for maintaining cell integrity and for promoting longevity. ..
  17. Maillet L, Tu C, Hong Y, Shuster E, Collart M. The essential function of Not1 lies within the Ccr4-Not complex. J Mol Biol. 2000;303:131-43 pubmed
    The five Saccharomyces cerevisiae Not proteins are associated with the Ccr4 and Caf1 proteins in 1.2 MDa and 2 MDa complexes...
  18. Hook B, Goldstrohm A, Seay D, Wickens M. Two yeast PUF proteins negatively regulate a single mRNA. J Biol Chem. 2007;282:15430-8 pubmed
    ..Combinatorial action of the two regulatory proteins may allow responses to specific environmental cues and be common in 3'-untranslated region-mediated control. ..
  19. Albert T, Lemaire M, van Berkum N, Gentz R, Collart M, Timmers H. Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits. Nucleic Acids Res. 2000;28:809-17 pubmed
    The yeast CCR4-NOT protein complex is a global regulator of RNA polymerase II transcription. It is comprised of yeast NOT1 to NOT5, yeast CCR4 and additional proteins like yeast CAF1...
  20. Woolstencroft R, Beilharz T, Cook M, Preiss T, Durocher D, Tyers M. Ccr4 contributes to tolerance of replication stress through control of CRT1 mRNA poly(A) tail length. J Cell Sci. 2006;119:5178-92 pubmed
    ..Strains bearing deletions in either CCR4 or CAF1/POP2, which encode components of the cytoplasmic mRNA deadenylase complex, were particularly sensitive to ..
  21. Lenssen E, James N, Pedruzzi I, Dubouloz F, Cameroni E, Bisig R, et al. The Ccr4-Not complex independently controls both Msn2-dependent transcriptional activation--via a newly identified Glc7/Bud14 type I protein phosphatase module--and TFIID promoter distribution. Mol Cell Biol. 2005;25:488-98 pubmed
    The Ccr4-Not complex is a conserved global regulator of gene expression, which serves as a regulatory platform that senses and/or transmits nutrient and stress signals to various downstream effectors...
  22. Draper M, Salvadore C, Denis C. Identification of a mouse protein whose homolog in Saccharomyces cerevisiae is a component of the CCR4 transcriptional regulatory complex. Mol Cell Biol. 1995;15:3487-95 pubmed
    The CCR4 protein from Saccharomyces cerevisiae is a component of a multisubunit complex that is required for the regulation of a number of genes in yeast cells...
  23. Panasenko O, Landrieux E, Feuermann M, Finka A, Paquet N, Collart M. The yeast Ccr4-Not complex controls ubiquitination of the nascent-associated polypeptide (NAC-EGD) complex. J Biol Chem. 2006;281:31389-98 pubmed
    In this work, we determine that the Saccharomyces cerevisiae Ccr4-Not complex controls ubiquitination of the conserved ribosome-associated heterodimeric EGD (enhancer of Gal4p DNA binding) complex, which consists of the Egd1p and Egd2p ..
  24. Tucker M, Valencia Sanchez M, Staples R, Chen J, Denis C, Parker R. The transcription factor associated Ccr4 and Caf1 proteins are components of the major cytoplasmic mRNA deadenylase in Saccharomyces cerevisiae. Cell. 2001;104:377-86 pubmed
    ..We demonstrate by several criteria that CCR4 and CAF1 encode critical components of the major cytoplasmic deadenylase in yeast...
  25. Maillet L, Collart M. Interaction between Not1p, a component of the Ccr4-not complex, a global regulator of transcription, and Dhh1p, a putative RNA helicase. J Biol Chem. 2002;277:2835-42 pubmed
    The Ccr4-Not complex is a global regulator of transcription that affects genes positively and negatively and is thought to regulate transcription factor IID function...
  26. Chen J, Rappsilber J, Chiang Y, Russell P, Mann M, Denis C. Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. J Mol Biol. 2001;314:683-94 pubmed
    The CCR4-NOT complex is an evolutionarily conserved, transcriptional regulatory complex that is involved in controlling mRNA initiation, elongation and degradation...
  27. Beckham C, Hilliker A, Cziko A, Noueiry A, Ramaswami M, Parker R. The DEAD-box RNA helicase Ded1p affects and accumulates in Saccharomyces cerevisiae P-bodies. Mol Biol Cell. 2008;19:984-93 pubmed
    ..Combined with earlier work showing Ded1p can have a positive effect on translation, these results suggest that Ded1p is a bifunctional protein that can affect both translation initiation and P-body formation. ..
  28. Gaillard H, Tous C, Botet J, González Aguilera C, Quintero M, Viladevall L, et al. Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair. PLoS Genet. 2009;5:e1000364 pubmed publisher
    ..Our data provide evidence that subunits of the SAGA and Ccr4-Not complexes, Mediator, Bre1, Bur2, and Fun12 affect transcription elongation to different extents...
  29. Assenholt J, Mouaikel J, Saguez C, Rougemaille M, Libri D, Jensen T. Implication of Ccr4-Not complex function in mRNA quality control in Saccharomyces cerevisiae. RNA. 2011;17:1788-94 pubmed publisher
    ..The Ccr4-Not complex, which constitutes the major S...
  30. Davidson M, Katou Y, Keszthelyi A, Sing T, Xia T, Ou J, et al. Endogenous DNA replication stress results in expansion of dNTP pools and a mutator phenotype. EMBO J. 2012;31:895-907 pubmed publisher
    ..Together, our data point to a vicious circle in which mutations in gatekeeper genes give rise to genomic instability during S phase, inducing expansion of the dNTP pool, which in turn results in high levels of spontaneous mutagenesis. ..
  31. Villanyi Z, Collart M. Ccr4-Not is at the core of the eukaryotic gene expression circuitry. Biochem Soc Trans. 2015;43:1253-8 pubmed publisher
    ..We introduce the Ccr4 (carbon catabolite repressed 4)-Not (negative on TATA-less) complex as a candidate to be a master regulator that ..
  32. Kassem S, Villanyi Z, Collart M. Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA. Nucleic Acids Res. 2017;45:1186-1199 pubmed publisher
    ..Previous screens have determined that global acetylation is reduced in cells lacking subunits of the Ccr4–Not complex, a global regulator of eukaryotic gene expression...
  33. Liu H, Toyn J, Chiang Y, Draper M, Johnston L, Denis C. DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex. EMBO J. 1997;16:5289-98 pubmed
    b>CCR4, a general transcriptional regulator affecting the expression of a number of genes in yeast, forms a multi-subunit complex in vivo...
  34. Laribee R, Hosni Ahmed A, Workman J, Chen H. Ccr4-not regulates RNA polymerase I transcription and couples nutrient signaling to the control of ribosomal RNA biogenesis. PLoS Genet. 2015;11:e1005113 pubmed publisher
    ..This study provides evidence that the Ccr4-Not complex, which regulates RNA Polymerase II (Pol II) transcription, also functions downstream of mTORC1 to ..
  35. Dutko J, Kenny A, Gamache E, Curcio M. 5' to 3' mRNA decay factors colocalize with Ty1 gag and human APOBEC3G and promote Ty1 retrotransposition. J Virol. 2010;84:5052-66 pubmed publisher
    ..Together, the results suggest that VLPs assemble in P bodies and that 5' to 3' mRNA decay is essential for the packaging of Ty1 RNA in VLPs. ..
  36. Simon E, Seraphin B. A specific role for the C-terminal region of the Poly(A)-binding protein in mRNA decay. Nucleic Acids Res. 2007;35:6017-28 pubmed
    ..vitro analyses demonstrate that removal of the Pab1 linker domain directly interferes with the ability of the Pop2-Ccr4 complex to deadenylate the Pab1-bound poly(A)...
  37. Cooper K, Scarnati M, Krasley E, Mallory M, Jin C, Law M, et al. Oxidative-stress-induced nuclear to cytoplasmic relocalization is required for Not4-dependent cyclin C destruction. J Cell Sci. 2012;125:1015-26 pubmed publisher
    ..These results identify a stress-induced proteolytic pathway regulating cyclin C that requires nuclear to cytoplasmic relocalization and Not4p-mediated ubiquitylation. ..
  38. Denis C. Identification of new genes involved in the regulation of yeast alcohol dehydrogenase II. Genetics. 1984;108:833-44 pubmed
    ..A third locus, CCR4, was identified by recessive mutations that suppressed the cre1 and cre2 phenotypes...
  39. Norbeck J. Carbon source dependent dynamics of the Ccr4-Not complex in Saccharomyces cerevisiae. J Microbiol. 2008;46:692-6 pubmed publisher
    We have investigated the composition of the conserved Ccr4-Not complex during different physiological states of Saccharomyces cerevisiae...
  40. Stowell J, Webster M, Kögel A, Wolf J, Shelley K, Passmore L. Reconstitution of Targeted Deadenylation by the Ccr4-Not Complex and the YTH Domain Protein Mmi1. Cell Rep. 2016;17:1978-1989 pubmed publisher
    b>Ccr4-Not is a conserved protein complex that shortens the 3' poly(A) tails of eukaryotic mRNAs to regulate transcript stability and translation into proteins...
  41. Malvar T, Biron R, Kaback D, Denis C. The CCR4 protein from Saccharomyces cerevisiae contains a leucine-rich repeat region which is required for its control of ADH2 gene expression. Genetics. 1992;132:951-62 pubmed
    The CCR4 gene from Saccharomyces cerevisiae is required for the transcription of the glucose-repressible alcohol dehydrogenase (ADH2)...
  42. Traven A, Lo T, Lithgow T, Heierhorst J. The yeast PUF protein Puf5 has Pop2-independent roles in response to DNA replication stress. PLoS ONE. 2010;5:e10651 pubmed publisher
    ..Yeast Puf5 is the prototype for studying PUF-dependent gene repression. Puf5 binds to the Pop2 subunit of the Ccr4-Pop2-NOT mRNA deadenylase, recruiting the deadenylase and associated translational repressors to mRNAs...
  43. Rendl L, Bieman M, Smibert C. S. cerevisiae Vts1p induces deadenylation-dependent transcript degradation and interacts with the Ccr4p-Pop2p-Not deadenylase complex. RNA. 2008;14:1328-36 pubmed publisher
    ..Taken together with previous work, these data suggest that Smaug family members employ a conserved mechanism to induce transcript degradation that involves recruitment of the Ccr4-Pop2-Not deadenylase to target mRNAs.
  44. Bennett C, Westmoreland T, Verrier C, Blanchette C, Sabin T, Phatnani H, et al. Yeast screens identify the RNA polymerase II CTD and SPT5 as relevant targets of BRCA1 interaction. PLoS ONE. 2008;3:e1448 pubmed publisher
    ..ASM4, MLP1, MLP2, NUP2, NUP53, NUP120, NUP133, NUP170, NUP188, POM34) and cytoplasmic mRNA decay at P-bodies (CCR4, DHH1)...
  45. Clark L, Viswanathan P, Quigley G, Chiang Y, McMahon J, Yao G, et al. Systematic mutagenesis of the leucine-rich repeat (LRR) domain of CCR4 reveals specific sites for binding to CAF1 and a separate critical role for the LRR in CCR4 deadenylase activity. J Biol Chem. 2004;279:13616-23 pubmed
    b>CCR4, a poly(A) deadenylase of the exonuclease III family, is a component of the multiprotein CCR4-NOT complex of Saccharomyces cerevisiae that is involved in mRNA degradation...
  46. Kimura Y, Irie K, Irie K. Pbp1 is involved in Ccr4- and Khd1-mediated regulation of cell growth through association with ribosomal proteins Rpl12a and Rpl12b. Eukaryot Cell. 2013;12:864-74 pubmed publisher
    ..In this study, we showed that PBP1 genetically interacts with CCR4 and KHD1, which encode a cytoplasmic deadenylase and an RNA-binding protein, respectively...
  47. Dutta A, Babbarwal V, Fu J, Brunke Reese D, Libert D, Willis J, et al. Ccr4-Not and TFIIS Function Cooperatively To Rescue Arrested RNA Polymerase II. Mol Cell Biol. 2015;35:1915-25 pubmed publisher
    ..The Ccr4-Not complex participates in multiple steps of mRNA metabolism and has recently been shown to be an EF...
  48. Nasertorabi F, Batisse C, Diepholz M, Suck D, Böttcher B. Insights into the structure of the CCR4-NOT complex by electron microscopy. FEBS Lett. 2011;585:2182-6 pubmed publisher
    The CCR4-NOT complex is a deadenylation complex, which plays a major role for mRNA stability. The complex is conserved from yeast to human and consists of nine proteins NOT1-NOT5, CCR4, CAF1, CAF40 and CAF130...
  49. Makino S, Mishima Y, Inoue K, Inada T. Roles of mRNA fate modulators Dhh1 and Pat1 in TNRC6-dependent gene silencing recapitulated in yeast. J Biol Chem. 2015;290:8331-47 pubmed publisher
    The CCR4-NOT complex, the major deadenylase in eukaryotes, plays crucial roles in gene expression at the levels of transcription, mRNA decay, and protein degradation...
  50. Reese J. The control of elongation by the yeast Ccr4-not complex. Biochim Biophys Acta. 2013;1829:127-33 pubmed publisher
    The Ccr4-Not complex is a highly conserved nine-subunit protein complex that has been implicated in virtually all aspects of gene control, including transcription, mRNA decay and quality control, RNA export, translational repression and ..
  51. Piening B, Huang D, Paulovich A. Novel connections between DNA replication, telomere homeostasis, and the DNA damage response revealed by a genome-wide screen for TEL1/ATM interactions in Saccharomyces cerevisiae. Genetics. 2013;193:1117-33 pubmed publisher
  52. Castells Roca L, Muhlenhoff U, Lill R, Herrero E, Belli G. The oxidative stress response in yeast cells involves changes in the stability of Aft1 regulon mRNAs. Mol Microbiol. 2011;81:232-48 pubmed publisher
    ..cerevisiae cells redirect iron assimilation through the non-reductive pathway to minimize oxidative damage by the ferrous ions, which are formed during iron import through the Ftr1/Fet3 complexes. ..
  53. Lau N, Mulder K, Brenkman A, Mohammed S, van den Broek N, Heck A, et al. Phosphorylation of Not4p functions parallel to BUR2 to regulate resistance to cellular stresses in Saccharomyces cerevisiae. PLoS ONE. 2010;5:e9864 pubmed publisher
    The evolutionarily conserved Ccr4-Not and Bur1/2 kinase complexes are functionally related in Saccharomyces cerevisiae. In this study, we further explore the relationship between the subunits Not4p and Bur2p...
  54. Luhtala N, Parker R. LSM1 over-expression in Saccharomyces cerevisiae depletes U6 snRNA levels. Nucleic Acids Res. 2009;37:5529-36 pubmed publisher
    ..These results suggest that inhibition of splicing and/or deadenylation may be effective therapies for Lsm1-over-expressing tumors. ..
  55. Denis C, Draper M, Liu H, Malvar T, Vallari R, Cook W. The yeast CCR4 protein is neither regulated by nor associated with the SPT6 and SPT10 proteins and forms a functionally distinct complex from that of the SNF/SWI transcription factors. Genetics. 1994;138:1005-13 pubmed
    The CCR4 protein is specifically required for the increased transcription at the ADH2 locus resulting from mutations in the SPT10 (CRE1) and SPT6 (CRE2) genes and is also required for the expression of ADH2 and other genes under non-..
  56. Lee D, Ohn T, Chiang Y, Quigley G, Yao G, Liu Y, et al. PUF3 acceleration of deadenylation in vivo can operate independently of CCR4 activity, possibly involving effects on the PAB1-mRNP structure. J Mol Biol. 2010;399:562-75 pubmed publisher
    The evolutionarily conserved PUF proteins stimulate CCR4 mRNA deadenylation through binding to 3' untranslated region sequences of specific mRNA...
  57. Luo X, Talarek N, De Virgilio C. Initiation of the yeast G0 program requires Igo1 and Igo2, which antagonize activation of decapping of specific nutrient-regulated mRNAs. RNA Biol. 2011;8:14-7 pubmed
    ..Here, we show that several factors including Ccr4, the Lsm-Pat1 complex, and Dhh1, which are implicated in mRNA decapping activation, participate in the decay of ..
  58. Schild D. Suppression of a new allele of the yeast RAD52 gene by overexpression of RAD51, mutations in srs2 and ccr4, or mating-type heterozygosity. Genetics. 1995;140:115-27 pubmed
    ..A new allele of srs2 and alleles of the transcriptional regulatory genes ccr4 and caf1 were among the other extragenic revertants of rad52-20...
  59. Kruk J, Dutta A, Fu J, Gilmour D, Reese J. The multifunctional Ccr4-Not complex directly promotes transcription elongation. Genes Dev. 2011;25:581-93 pubmed publisher
    The Ccr4-Not complex has been implicated in the control of multiple steps of mRNA metabolism; however, its functions in transcription remain ambiguous...
  60. Lo T, Qu Y, Uwamahoro N, Quenault T, Beilharz T, Traven A. The mRNA decay pathway regulates the expression of the Flo11 adhesin and biofilm formation in Saccharomyces cerevisiae. Genetics. 2012;191:1387-91 pubmed publisher
    ..We establish that the major mRNA decay pathway regulates FLO11 expression. mRNA deadenylation of transcriptional repressors of FLO11 by the exonuclease Ccr4 keeps their levels low, thereby allowing FLO11 transcription.
  61. Tomar R, Zheng S, Brunke Reese D, Wolcott H, Reese J. Yeast Rap1 contributes to genomic integrity by activating DNA damage repair genes. EMBO J. 2008;27:1575-84 pubmed publisher
    ..We propose that Rap1 acts as a rheostat controlling nucleotide pools in response to shortened telomeres and DNA damage, providing a mechanism for fine-tuning the RNR genes during checkpoint activation. ..
  62. Huch S, Müller M, Muppavarapu M, Gommlich J, Balagopal V, Nissan T. The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae. Biol Open. 2016;5:1388-1399 pubmed publisher
    ..The destabilization was linked to an increased dependence on Ccr4-mediated deadenylation and mRNA decapping...
  63. Raju K, Natarajan S, Kumar N, Kumar D, N M R. Role of cytoplasmic deadenylation and mRNA decay factors in yeast apoptosis. FEMS Yeast Res. 2015;15: pubmed publisher
    ..factors (DCP2 and LSM1), cytoplasmic exosome function (SKI2) or cytoplasmic deadenylases (double deletion of CCR4 and PAN2) showed typical markers of eukaryotic apoptosis such as increased cellular reactive oxygen species levels, ..
  64. Tabtiang R, Herskowitz I. Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae. Mol Cell Biol. 1998;18:4707-18 pubmed
    ..NUT1 causes a constitutive, Swi4p-independent phenotype only in combination with the nut2-1 allele or an allele of CCR4. In contrast, inactivation of a temperature-sensitive allele of NUT2, nut2-ts70, alone causes constitutivity...