Gene Symbol: CCP1
Description: cytochrome-c peroxidase
Alias: cytochrome-c peroxidase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Bender T, Leidhold C, Ruppert T, Franken S, Voos W. The role of protein quality control in mitochondrial protein homeostasis under oxidative stress. Proteomics. 2010;10:1426-43 pubmed publisher
    ..As Pim1/LON expression was induced significantly under ROS treatment, we propose that this protease system performs a crucial protective function under oxidative stress conditions. ..
  2. Houseley J, Tollervey D. Yeast Trf5p is a nuclear poly(A) polymerase. EMBO Rep. 2006;7:205-11 pubmed
    ..Trf5p co-purified with Mtr4p and Air1p, indicating that it forms a complex, designated TRAMP5, that has functions that partially overlap with the TRAMP complex. ..
  3. Paul S, Sil P, Chakraborty R, Haldar S, Chattopadhyay K. Molecular Crowding Affects the Conformational Fluctuations, Peroxidase Activity, and Folding Landscape of Yeast Cytochrome c. Biochemistry. 2016;55:2332-43 pubmed publisher
    ..Interestingly, the time constant of conformational fluctuations between the compact and expanded conformers has been found to increase in the crowded milieu, suggesting a crucial role of the solution microviscosity. ..
  4. Nakani S, Viriyakul T, Mitchell R, Vitello L, Erman J. Characterization of a covalently linked yeast cytochrome c-cytochrome c peroxidase complex: evidence for a single, catalytically active cytochrome c binding site on cytochrome c peroxidase. Biochemistry. 2006;45:9887-93 pubmed
    A covalent complex between recombinant yeast iso-1-cytochrome c and recombinant yeast cytochrome c peroxidase (rCcP), in which the crystallographically defined cytochrome c binding site [Pelletier, H., and Kraut, J...
  5. He X, Fassler J. Identification of novel Yap1p and Skn7p binding sites involved in the oxidative stress response of Saccharomyces cerevisiae. Mol Microbiol. 2005;58:1454-67 pubmed
    ..In this study, the complex, oxidative responsive, CCP1 promoter was used as a model to investigate the cis-acting elements responsible for activation by oxidative stress...
  6. Mei H, Geren L, Miller M, Durham B, Millett F. Role of the low-affinity binding site in electron transfer from cytochrome C to cytochrome C peroxidase. Biochemistry. 2002;41:3968-76 pubmed
    The interaction of yeast iso-1-cytochrome c (yCc) with the high- and low-affinity binding sites on cytochrome c peroxidase compound I (CMPI) was studied by stopped-flow spectroscopy. When 3 microM reduced yCc(II) was mixed with 0...
  7. Böttinger L, Gornicka A, Czerwik T, Bragoszewski P, Loniewska Lwowska A, Schulze Specking A, et al. In vivo evidence for cooperation of Mia40 and Erv1 in the oxidation of mitochondrial proteins. Mol Biol Cell. 2012;23:3957-69 pubmed publisher
    ..Thus Mia40 in cooperation with Erv1 promotes the formation of two disulfide bonds in the substrate protein, ensuring the efficiency of oxidative folding in the intermembrane space of mitochondria. ..
  8. Sterckx Y, Volkov A. Cofactor-dependent structural and binding properties of yeast cytochrome C peroxidase. Biochemistry. 2014;53:4526-36 pubmed publisher
    Yeast cytochrome c peroxidase (CcP) is a heme enzyme that reduces hydroperoxides using the electrons provided by its physiological partner cytochrome c (Cc)...
  9. Pearl N, Jacobson T, Arisa M, Vitello L, Erman J. Effect of single-site charge-reversal mutations on the catalytic properties of yeast cytochrome c peroxidase: mutations near the high-affinity cytochrome c binding site. Biochemistry. 2007;46:8263-72 pubmed
    Fifteen single-site charge-reversal mutations of yeast cytochrome c peroxidase (CcP) have been constructed to determine the effect of localized charge on the catalytic properties of the enzyme...

More Information


  1. Charizanis C, Juhnke H, Krems B, Entian K. The mitochondrial cytochrome c peroxidase Ccp1 of Saccharomyces cerevisiae is involved in conveying an oxidative stress signal to the transcription factor Pos9 (Skn7). Mol Gen Genet. 1999;262:437-47 pubmed
    ..fap24, which was respiration-competent, could be complemented by CCP1, which encodes the mitochondrial cytochrome c peroxidase. Mitochondrial cytochrome c peroxidase degrades reactive oxygen species within the mitochondria...
  2. Waldmeyer B, Bechtold R, Bosshard H, Poulos T. The cytochrome c peroxidase.cytochrome c electron transfer complex. Experimental support of a hypothetical model. J Biol Chem. 1982;257:6073-6 pubmed
    A hypothetical model of the cytochrome c peroxidase.cytochrome c complex (Poulos, T. L., and Kraut, J. (1980) J. Biol. Chem...
  3. Morar A, Wang X, Pielak G. Effects of crowding by mono-, di-, and tetrasaccharides on cytochrome c-cytochrome c peroxidase binding: comparing experiment to theory. Biochemistry. 2001;40:281-5 pubmed
    ..None of the analyses are completely successful by themselves, and the results suggest that a complete analysis must account for both excluded-volume and chemical interactions. ..
  4. DiCarlo C, Vitello L, Erman J. Reduction potential of yeast cytochrome c peroxidase and three distal histidine mutants: dependence on pH. J Inorg Biochem. 2011;105:532-7 pubmed publisher
    The pH dependence of the Fe(III) reduction potential, E(0)', for yeast cytochrome c peroxidase (yCcP) and three distal pocket mutants, CcP(H52L), CcP(H52Q), and CcP(R48L/W51L/H52L), has been determined between pH 4 and 8...
  5. Suppanz I, Wurm C, Wenzel D, Jakobs S. The m-AAA protease processes cytochrome c peroxidase preferentially at the inner boundary membrane of mitochondria. Mol Biol Cell. 2009;20:572-80 pubmed publisher
    ..We have analyzed the localizations of the m-AAA protease and its substrate cytochrome c peroxidase (Ccp1) within yeast mitochondria using live cell fluorescence microscopy and quantitative immunoelectron ..
  6. Martins D, Kathiresan M, English A. Cytochrome c peroxidase is a mitochondrial heme-based H2O2 sensor that modulates antioxidant defense. Free Radic Biol Med. 2013;65:541-551 pubmed publisher
    ..Here we provide substantial biochemical evidence that the heme enzyme Ccp1 (cytochrome c peroxidase), which is targeted to the intermembrane space, functions primarily as a mitochondrial H2O2 sensing and ..
  7. Dabir D, Leverich E, Kim S, Tsai F, Hirasawa M, Knaff D, et al. A role for cytochrome c and cytochrome c peroxidase in electron shuttling from Erv1. EMBO J. 2007;26:4801-11 pubmed
    ..oxygen as an electron acceptor to generate hydrogen peroxide, which is subsequently reduced to water by cytochrome c peroxidase (Ccp1). Oxidized Ccp1 was in turn reduced by the Erv1-reduced cytochrome c...
  8. Bashir Q, Meulenbroek E, Pannu N, Ubbink M. Engineering specificity in a dynamic protein complex with a single conserved mutation. FEBS J. 2014;281:4892-905 pubmed publisher
    It has been demonstrated that the complex of yeast cytochrome c (Cc) and cytochrome c peroxidase (CcP) exists as a delicate equilibrium of a specific, active state and the non-specific, dynamic encounter state...
  9. Page T, Hoffman B. Control of cyclic photoinitiated electron transfer between cytochrome c peroxidase (W191F) and cytochrome c by formation of dynamic binary and ternary complexes. Biochemistry. 2015;54:1188-97 pubmed publisher
    Extensive studies of the physiological protein-protein electron-transfer (ET) complex between yeast cytochrome c peroxidase (CcP) and cytochrome c (Cc) have left unresolved questions about how formation and dissociation of binary and ..
  10. Erman J, Vitello L, Pearl N, Jacobson T, Francis M, Alberts E, et al. Binding of Yeast Cytochrome c to Forty-Four Charge-Reversal Mutants of Yeast Cytochrome c Peroxidase: Isothermal Titration Calorimetry. Biochemistry. 2015;54:4845-54 pubmed publisher
    Previously, we constructed, expressed, and purified 46 charge-reversal mutants of yeast cytochrome c peroxidase (CcP) and determined their electronic absorption spectra, their reaction with H2O2, and their steady-state catalytic ..
  11. Daum G, Böhni P, Schatz G. Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria. J Biol Chem. 1982;257:13028-33 pubmed
    ..Cytochrome b2 and cytochrome c peroxidase were found to be components of the intermembrane space...
  12. McQuibban G, Saurya S, Freeman M. Mitochondrial membrane remodelling regulated by a conserved rhomboid protease. Nature. 2003;423:537-41 pubmed
    ..We have identified two substrates of Rbd1p: cytochrome c peroxidase (Ccp1p); and a dynamin-like GTPase (Mgm1p), which is involved in mitochondrial membrane fusion...
  13. Shen D, Yuan H, Hutagalung A, Verma A, Kümmel D, Wu X, et al. The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p. J Cell Biol. 2013;202:509-26 pubmed publisher
    ..We propose that the exocyst is recruited to secretory vesicles by the combinatorial signals of Sec4-GTP and the Snc proteins. This could help to confer both specificity and directionality to vesicular traffic. ..
  14. Kathiresan M, Martins D, English A. Respiration triggers heme transfer from cytochrome c peroxidase to catalase in yeast mitochondria. Proc Natl Acad Sci U S A. 2014;111:17468-73 pubmed publisher
    In exponentially growing yeast, the heme enzyme, cytochrome c peroxidase (Ccp1) is targeted to the mitochondrial intermembrane space...
  15. Bidwai A, Ayala C, Vitello L, Erman J. Apolar distal pocket mutants of yeast cytochrome c peroxidase: Binding of imidazole, 1-methylimidazole and 4-nitroimidazole to the triAla, triVal, and triLeu variants. Biochim Biophys Acta. 2015;1854:919-29 pubmed publisher
    Imidazole binding to three apolar distal heme pocket mutants of yeast cytochrome c peroxidase (CcP) has been investigated between pH4 and 8...
  16. Erman J, Chinchilla D, Studer J, Vitello L. Binding of imidazole, 1-methylimidazole and 4-nitroimidazole to yeast cytochrome c peroxidase (CcP) and the distal histidine mutant, CcP(H52L). Biochim Biophys Acta. 2015;1854:869-81 pubmed publisher
    Imidazole, 1-methylimidazole and 4-nitroimidazole bind to yeast cytochrome c peroxidase (yCcP) with apparent equilibrium dissociation constants (KD(app)) of 3.3±0.4, 0.85±0.11, and ~0.2M, respectively, at pH7...
  17. Nakani S, Vitello L, Erman J. Characterization of four covalently-linked yeast cytochrome c/cytochrome c peroxidase complexes: Evidence for electrostatic interaction between bound cytochrome c molecules. Biochemistry. 2006;45:14371-8 pubmed
    Four covalent complexes between recombinant yeast cytochrome c and cytochrome c peroxidase (rCcP) were synthesized via disulfide bond formation using specifically designed protein mutants (Papa, H. S., and Poulos, T. L...