Gene Symbol: CCC1
Description: Ccc1p
Alias: Ccc1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Li L, Murdock G, Bagley D, Jia X, Ward D, Kaplan J. Genetic dissection of a mitochondria-vacuole signaling pathway in yeast reveals a link between chronic oxidative stress and vacuolar iron transport. J Biol Chem. 2010;285:10232-42 pubmed publisher
    ..MRS3 and MRS4, that encode mitochondrial iron transporters affects the activity of the vacuolar iron importer Ccc1. Ccc1 levels are decreased in Deltamrs3Deltamrs4 cells, but the activity of the transporter is increased, resulting ..
  2. Li L, Jia X, Ward D, Kaplan J. Yap5 protein-regulated transcription of the TYW1 gene protects yeast from high iron toxicity. J Biol Chem. 2011;286:38488-97 pubmed publisher
    ..We identified a minimal domain of S. cerevisiae Tyw1 that is sufficient to induce the iron regulon. CCC1, a vacuolar iron importer, is a Yap5-regulated gene, and deletion of either CCC1 or YAP5 resulted in high iron ..
  3. Li L, Kaplan J, Ward D. The glucose sensor Snf1 and the transcription factors Msn2 and Msn4 regulate transcription of the vacuolar iron importer gene CCC1 and iron resistance in yeast. J Biol Chem. 2017;292:15577-15586 pubmed publisher
    ..One key protein involved in vacuolar iron storage is the iron importer Ccc1, which facilitates iron entry into the vacuole...
  4. Kumanovics A, Poruk K, Osborn K, Ward D, Kaplan J. YKE4 (YIL023C) encodes a bidirectional zinc transporter in the endoplasmic reticulum of Saccharomyces cerevisiae. J Biol Chem. 2006;281:22566-74 pubmed
  5. Li L, Miao R, Bertram S, Jia X, Ward D, Kaplan J. A role for iron-sulfur clusters in the regulation of transcription factor Yap5-dependent high iron transcriptional responses in yeast. J Biol Chem. 2012;287:35709-21 pubmed publisher
    Yeast respond to increased cytosolic iron by activating the transcription factor Yap5 increasing transcription of CCC1, which encodes a vacuolar iron importer...
  6. Fu D, Beeler T, Dunn T. Sequence, mapping and disruption of CCC1, a gene that cross-complements the Ca(2+)-sensitive phenotype of csg1 mutants. Yeast. 1994;10:515-21 pubmed
    We have isolated, sequenced, mapped and disrupted a novel gene, CCC1, from Saccharomyces cerevisiae. This gene displays non-allelic complementation of the Ca(2+)-sensitive phenotype conferred by the csg1 mutation...
  7. Xue Y, Schmollinger S, Attar N, Campos O, Vogelauer M, Carey M, et al. Endoplasmic reticulum-mitochondria junction is required for iron homeostasis. J Biol Chem. 2017;292:13197-13204 pubmed publisher
    ..Our findings reveal that proper communication between the ER and mitochondria is required for appropriate maintenance of cellular iron levels. ..
  8. Li L, Chen O, McVey Ward D, Kaplan J. CCC1 is a transporter that mediates vacuolar iron storage in yeast. J Biol Chem. 2001;276:29515-9 pubmed
    ..Using several different criteria we demonstrated that CCC1 encodes a transporter that effects the accumulation of iron and Mn(2+) in vacuoles...
  9. Li L, Bagley D, Ward D, Kaplan J. Yap5 is an iron-responsive transcriptional activator that regulates vacuolar iron storage in yeast. Mol Cell Biol. 2008;28:1326-37 pubmed
    The transporter Ccc1 imports iron into the vacuole, which is the major site of iron storage in fungi and plants. CCC1 mRNA is destabilized under low-iron conditions by the binding of Cth1 and Cth2 to the 3' untranslated region (S. Puig, E...

More Information


  1. Lee Y, Huang X, Kropat J, Henras A, Merchant S, Dickson R, et al. Sphingolipid signaling mediates iron toxicity. Cell Metab. 2012;16:90-6 pubmed publisher
    ..These results demonstrate an unexpected connection between sphingolipid flux and iron toxicity and show that activation of a signal transduction cascade contributes to iron-mediated cellular toxicity. ..
  2. Pozos T, Sekler I, Cyert M. The product of HUM1, a novel yeast gene, is required for vacuolar Ca2+/H+ exchange and is related to mammalian Na+/Ca2+ exchangers. Mol Cell Biol. 1996;16:3730-41 pubmed
    ..We show that calcineurin mutants are sensitive to high concentrations of Mn2+ and identify two genes, CCC1 and HUM1, that, at high dosages, increase the Mn2+ tolerance of calcineurin mutants...
  3. Matsumoto R, Suzuki K, Ohya Y. Organelle acidification is important for localisation of vacuolar proteins in Saccharomyces cerevisiae. Protoplasma. 2013;250:1283-93 pubmed publisher
    ..These results suggest that many alterations in the localisation of vacuolar proteins occur after loss of the acidification of acidic compartments. ..
  4. Li L, Kaplan J. A mitochondrial-vacuolar signaling pathway in yeast that affects iron and copper metabolism. J Biol Chem. 2004;279:33653-61 pubmed
    ..Cells with a deletion in the vacuolar iron/manganese transporter Ccc1p are sensitive to high iron. Overexpression of MRS3 or MRS4 suppresses the high iron sensitivity of Deltaccc1 cells...
  5. Lapinskas P, Lin S, Culotta V. The role of the Saccharomyces cerevisiae CCC1 gene in the homeostasis of manganese ions. Mol Microbiol. 1996;21:519-28 pubmed
    ..Our studies indicate that, like PMR1, CCC1 functions in the homeostasis of both calcium and manganese ions. The Ccc1p polypeptide was found to localize to a Golgi-like organelle in yeast cells...
  6. Lin H, Li L, Jia X, Ward D, Kaplan J. Genetic and biochemical analysis of high iron toxicity in yeast: iron toxicity is due to the accumulation of cytosolic iron and occurs under both aerobic and anaerobic conditions. J Biol Chem. 2011;286:3851-62 pubmed publisher
    Iron storage in yeast requires the activity of the vacuolar iron transporter Ccc1. Yeast with an intact CCC1 are resistant to iron toxicity, but deletion of CCC1 renders yeast susceptible to iron toxicity...
  7. Lin H, Kumanovics A, Nelson J, Warner D, Ward D, Kaplan J. A single amino acid change in the yeast vacuolar metal transporters ZRC1 and COT1 alters their substrate specificity. J Biol Chem. 2008;283:33865-73 pubmed publisher
    ..The budding yeast Saccharomyces cerevisiae detoxifies cytosolic iron by storage in the vacuole. Deletion of CCC1, which encodes the vacuolar iron importer, results in high iron sensitivity due to increased cytosolic iron...
  8. Devasahayam G, Burke D, Sturgill T. Golgi manganese transport is required for rapamycin signaling in Saccharomyces cerevisiae. Genetics. 2007;177:231-8 pubmed
    ..Overexpression of Ccc1, a Fe2+ and Mn2+ transporter that has been localized to Golgi and the vacuole, does restore rapamycin sensitivity ..
  9. Li L, Miao R, Jia X, Ward D, Kaplan J. Expression of the yeast cation diffusion facilitators Mmt1 and Mmt2 affects mitochondrial and cellular iron homeostasis: evidence for mitochondrial iron export. J Biol Chem. 2014;289:17132-41 pubmed publisher
    ..This low iron transcriptional response was suppressed by deletion of CCC1, the gene that encodes the vacuolar iron importer...
  10. Chen O, Kaplan J. CCC1 suppresses mitochondrial damage in the yeast model of Friedreich's ataxia by limiting mitochondrial iron accumulation. J Biol Chem. 2000;275:7626-32 pubmed
    ..A suppressor screen identified a gene, CCC1, that maintained respiratory function in a Deltayfh1 yeast strain regardless of extracellular iron concentration...