Gene Symbol: CBK1
Description: serine/threonine protein kinase CBK1
Alias: serine/threonine protein kinase CBK1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Panozzo C, Bourens M, Nowacka A, Herbert C. Mutations in the C-terminus of the conserved NDR kinase, Cbk1p of Saccharomyces cerevisiae, make the protein independent of upstream activators. Mol Genet Genomics. 2010;283:111-22 pubmed publisher
    ..Our results show that the CBK1-S745F allele leads to a constitutively active form of Cbk1p that is independent of the upstream RAM network...
  2. Sbia M, Parnell E, Yu Y, Olsen A, Kretschmann K, Voth W, et al. Regulation of the yeast Ace2 transcription factor during the cell cycle. J Biol Chem. 2008;283:11135-45 pubmed publisher
    ..region of Ace2 as responsible for the delay, and this same region of Ace2 was required for interaction with Cbk1, a kinase necessary for both transcriptional activation by Ace2 and asymmetric distribution of Ace2...
  3. Racki W, Becam A, Nasr F, Herbert C. Cbk1p, a protein similar to the human myotonic dystrophy kinase, is essential for normal morphogenesis in Saccharomyces cerevisiae. EMBO J. 2000;19:4524-32 pubmed
    We have studied the CBK1 gene of Saccharomyces cerevisiae, which encodes a conserved protein kinase similar to the human myotonic dystrophy kinase...
  4. Bogomolnaya L, Pathak R, Guo J, Polymenis M. Roles of the RAM signaling network in cell cycle progression in Saccharomyces cerevisiae. Curr Genet. 2006;49:384-92 pubmed
    ..Taken together, our results point to a complex regulation of cell proliferation by RAM proteins, in a non-uniform manner that was not previously anticipated. ..
  5. Nelson B, Kurischko C, Horecka J, Mody M, Nair P, Pratt L, et al. RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. Mol Biol Cell. 2003;14:3782-803 pubmed
    ..Our data suggest that the functional architecture of RAM signaling is similar to the S. cerevisiae mitotic exit network and Schizosaccharomyces pombe septation initiation network and is likely conserved among eukaryotes. ..
  6. Weiss E, Kurischko C, Zhang C, Shokat K, Drubin D, Luca F. The Saccharomyces cerevisiae Mob2p-Cbk1p kinase complex promotes polarized growth and acts with the mitotic exit network to facilitate daughter cell-specific localization of Ace2p transcription factor. J Cell Biol. 2002;158:885-900 pubmed
    ..These findings may implicate related proteins in linking the regulation of cell morphology and cell cycle transitions with cell fate determination and development. ..
  7. Jorgensen P, Nelson B, Robinson M, Chen Y, Andrews B, Tyers M, et al. High-resolution genetic mapping with ordered arrays of Saccharomyces cerevisiae deletion mutants. Genetics. 2002;162:1091-9 pubmed
    ..The Cbk1 kinase signaling pathway, which consists minimally of CBK1, MOB2, KIC1, HYM1, and TAO3 (PAG1), controls polarized ..
  8. Kurischko C, Weiss G, Ottey M, Luca F. A role for the Saccharomyces cerevisiae regulation of Ace2 and polarized morphogenesis signaling network in cell integrity. Genetics. 2005;171:443-55 pubmed
    ..Collectively, these data indicate that RAM and Ssd1p function cooperatively to control cell integrity and suggest that Zrg8p and Srl1p function as nonessential inhibitors of Ssd1p. ..
  9. Kurischko C, Kuravi V, Wannissorn N, Nazarov P, Husain M, Zhang C, et al. The yeast LATS/Ndr kinase Cbk1 regulates growth via Golgi-dependent glycosylation and secretion. Mol Biol Cell. 2008;19:5559-78 pubmed publisher
    Saccharomyces cerevisiae Cbk1 is a LATS/Ndr protein kinase and a downstream component of the regulation of Ace2 and morphogenesis (RAM) signaling network...

More Information


  1. Du L, Novick P. Pag1p, a novel protein associated with protein kinase Cbk1p, is required for cell morphogenesis and proliferation in Saccharomyces cerevisiae. Mol Biol Cell. 2002;13:503-14 pubmed
    ..A mutant of PAG1, a novel gene in S. cerevisiae, displayed defects similar to those of cbk1 mutants...
  2. Colman Lerner A, Chin T, Brent R. Yeast Cbk1 and Mob2 activate daughter-specific genetic programs to induce asymmetric cell fates. Cell. 2001;107:739-50 pubmed
    In Saccharomyces cerevisiae, mothers and daughters have distinct fates. We show that Cbk1 kinase and its interacting protein Mob2 regulate this asymmetry by inducing daughter-specific genetic programs...
  3. Jansen J, Wanless A, Seidel C, Weiss E. Cbk1 regulation of the RNA-binding protein Ssd1 integrates cell fate with translational control. Curr Biol. 2009;19:2114-20 pubmed publisher
    ..In budding yeast, the conserved Ndr/warts kinase Cbk1 localizes to the new daughter cell, where it acts as a cell fate determinant...
  4. Mazanka E, Alexander J, Yeh B, Charoenpong P, Lowery D, Yaffe M, et al. The NDR/LATS family kinase Cbk1 directly controls transcriptional asymmetry. PLoS Biol. 2008;6:e203 pubmed publisher
    ..The NDR/LATS family protein kinase Cbk1 is required for Ace2 segregation and function...
  5. Bidlingmaier S, Weiss E, Seidel C, Drubin D, Snyder M. The Cbk1p pathway is important for polarized cell growth and cell separation in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:2449-62 pubmed
    ..The CBK1 gene, encoding a putative serine/threonine protein kinase, was identified in a screen designed to isolate mutations ..
  6. Kurischko C, Kuravi V, Herbert C, Luca F. Nucleocytoplasmic shuttling of Ssd1 defines the destiny of its bound mRNAs. Mol Microbiol. 2011;81:831-49 pubmed publisher
    ..Ssd1 is a substrate of the LATS/NDR tumour suppressor orthologue Cbk1 kinase...
  7. Brace J, Hsu J, Weiss E. Mitotic exit control of the Saccharomyces cerevisiae Ndr/LATS kinase Cbk1 regulates daughter cell separation after cytokinesis. Mol Cell Biol. 2011;31:721-35 pubmed publisher
    ..Genes involved in septum destruction are induced by the transcription factor Ace2, which is activated by the kinase Cbk1, an Ndr/LATS-related protein that functions in a system related to metazoan hippo pathways...
  8. Jansen J, Barry M, Yoo C, Weiss E. Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis. J Cell Biol. 2006;175:755-66 pubmed
    ..We find that phosphoregulation of the conserved AGC family kinase Cbk1 is critical for RAM network function...
  9. Bidlingmaier S, Snyder M. Large-scale identification of genes important for apical growth in Saccharomyces cerevisiae by directed allele replacement technology (DART) screening. Funct Integr Genomics. 2002;1:345-56 pubmed
    ..We identified 29 insertion alleles, containing mutations in 17 different genes ( SMY1, SPA2, PAN1, SLA1, SLA2, CBK1, SEC22, FAB1, VPS36, VID22, RAS2, ECM33, OPI3, API1/YDR372c, API2/YDR525w, API3/YKR020w, and API4/YNL051w), which ..
  10. Versele M, Thevelein J. Lre1 affects chitinase expression, trehalose accumulation and heat resistance through inhibition of the Cbk1 protein kinase in Saccharomyces cerevisiae. Mol Microbiol. 2001;41:1311-26 pubmed
    ..Lre1 has recently been isolated in a two-hybrid screen using the conserved protein kinase Cbk1 as a bait. Cbk1 controls the expression of CTS1 (encoding chitinase) through the transcription factor Ace2...
  11. Kaeberlein M, Guarente L. Saccharomyces cerevisiae MPT5 and SSD1 function in parallel pathways to promote cell wall integrity. Genetics. 2002;160:83-95 pubmed
    ..This work also provides evidence that post-transcriptional regulation is likely to be important both for maintaining cell integrity and for promoting longevity. ..
  12. Hsu J, Weiss E. Cell cycle regulated interaction of a yeast Hippo kinase and its activator MO25/Hym1. PLoS ONE. 2013;8:e78334 pubmed publisher
    ..of cell separation is phosphorylation of a conserved hydrophobic motif (HM) site on the NDR/LATS family kinase Cbk1. Here we provide the first direct evidence that the Hippo-like kinase Kic1 in fact phosphorylates the HM site of ..
  13. Wolfe K, Ren H, Trepte P, Cyr D. Polyglutamine-rich suppressors of huntingtin toxicity act upstream of Hsp70 and Sti1 in spatial quality control of amyloid-like proteins. PLoS ONE. 2014;9:e95914 pubmed publisher
    ..High copy suppressors of Htt103Q-NLS toxicity include the polyQ-domain containing proteins Nab3, Pop2, and Cbk1, and each suppresses Htt toxicity via a different mechanism...
  14. Krysan D, Ting E, Abeijon C, Kroos L, Fuller R. Yapsins are a family of aspartyl proteases required for cell wall integrity in Saccharomyces cerevisiae. Eukaryot Cell. 2005;4:1364-74 pubmed
    ..Taken together, these data indicate that the yapsins play an important role in glucan homeostasis in S. cerevisiae and that yapsin homologues may play a similar role in the pathogenic yeasts C. albicans and C. glabrata. ..
  15. Schneper L, Krauss A, Miyamoto R, Fang S, Broach J. The Ras/protein kinase A pathway acts in parallel with the Mob2/Cbk1 pathway to effect cell cycle progression and proper bud site selection. Eukaryot Cell. 2004;3:108-20 pubmed
    ..We have found that mutations in MOB2 or CBK1 confer a slow-growth phenotype in a ras2Delta background...
  16. Bourens M, Panozzo C, Nowacka A, Imbeaud S, Mucchielli M, Herbert C. Mutations in the Saccharomyces cerevisiae kinase Cbk1p lead to a fertility defect that can be suppressed by the absence of Brr1p or Mpt5p (Puf5p), proteins involved in RNA metabolism. Genetics. 2009;183:161-73 pubmed publisher
    ..We show here that some mutations of CBK1 also lead to a reduced fertility and a reduced expression of some of the mating type-specific genes...
  17. Geyer C, Colman Lerner A, Brent R. "Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections. Proc Natl Acad Sci U S A. 1999;96:8567-72 pubmed
    ..These experiments identified genes known to function in the pathway, as well as a protein kinase, the CBK1 product, whose function was not known...
  18. Mazanka E, Weiss E. Sequential counteracting kinases restrict an asymmetric gene expression program to early G1. Mol Biol Cell. 2010;21:2809-20 pubmed publisher
    ..The Ndr/LATS family kinase Cbk1, functioning in a system similar to metazoan hippo signaling pathways, activates Ace2 and drives its accumulation ..
  19. Kuravi V, Kurischko C, Puri M, Luca F. Cbk1 kinase and Bck2 control MAP kinase activation and inactivation during heat shock. Mol Biol Cell. 2011;22:4892-907 pubmed publisher
    Saccharomyces cerevisiae Cbk1 kinase is a LATS/NDR tumor suppressor orthologue and component of the Regulation of Ace2 and Morphogenesis signaling network...
  20. Mancini Lombardi I, Palani S, Meitinger F, Darieva Z, Hofmann A, Sharrocks A, et al. Lre1 directly inhibits the NDR/Lats kinase Cbk1 at the cell division site in a phosphorylation-dependent manner. Curr Biol. 2013;23:1736-45 pubmed publisher
    ..In budding yeast, the NDR kinase complex composed of Cbk1 and its regulatory subunit, Mob2, have an established role in the control of cell separation/abscission that ..
  21. Gógl G, Schneider K, Yeh B, Alam N, Nguyen Ba A, Moses A, et al. The Structure of an NDR/LATS Kinase-Mob Complex Reveals a Novel Kinase-Coactivator System and Substrate Docking Mechanism. PLoS Biol. 2015;13:e1002146 pubmed publisher
    ..Here we describe the crystal structure of budding yeast Cbk1-Mob2, to our knowledge the first of an NDR/LATS kinase-Mob complex...
  22. Voth W, Olsen A, Sbia M, Freedman K, Stillman D. ACE2, CBK1, and BUD4 in budding and cell separation. Eukaryot Cell. 2005;4:1018-28 pubmed
    Mutations in the RAM network genes, including CBK1, MOB2, KIC1, HYM1, and TAO3, cause defects in bud site selection, asymmetric apical growth, and mating projections...