Gene Symbol: CBF5
Description: pseudouridine synthase CBF5
Alias: pseudouridine synthase CBF5
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Cadwell C, Yoon H, Zebarjadian Y, Carbon J. The yeast nucleolar protein Cbf5p is involved in rRNA biosynthesis and interacts genetically with the RNA polymerase I transcription factor RRN3. Mol Cell Biol. 1997;17:6175-83 pubmed
    ..T. Meier, and G. Blobel, J. Cell Biol. 127:1505-1514, 1994). The temperature-sensitive cbf5-1 mutant demonstrates a pronounced defect in rRNA biosynthesis at restrictive temperatures, while tRNA ..
  2. Lafontaine D, Bousquet Antonelli C, Henry Y, Caizergues Ferrer M, Tollervey D. The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev. 1998;12:527-37 pubmed
    ..We conclude that Cbf5p is likely to be the rRNA Psi synthase and is an integral component of the box H + ACA class of snoRNPs, which function to target the enzyme to its site of action. ..
  3. Watkins N, Gottschalk A, Neubauer G, Kastner B, Fabrizio P, Mann M, et al. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure. RNA. 1998;4:1549-68 pubmed
    ..Electron microscopy of purified snR42 and snR30 particles revealed that these two snoRNPs possess a similar bipartite structure that we propose to be a major structural determining principle for all H/ACA snoRNPs. ..
  4. Fath S, Milkereit P, Podtelejnikov A, Bischler N, Schultz P, Bier M, et al. Association of yeast RNA polymerase I with a nucleolar substructure active in rRNA synthesis and processing. J Cell Biol. 2000;149:575-90 pubmed
    ..Our results support the idea that a functional nucleolar subdomain formed independently of the state of rDNA transcription may serve as a scaffold for coordinated rRNA synthesis and processing. ..
  5. Carlile T, Rojas Duran M, Zinshteyn B, Shin H, Bartoli K, Gilbert W. Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells. Nature. 2014;515:143-6 pubmed publisher
    ..Our findings reveal unanticipated roles for pseudouridylation and provide a resource for identifying the targets of pseudouridine synthases implicated in human disease. ..
  6. Schwartz S, Bernstein D, Mumbach M, Jovanovic M, Herbst R, León Ricardo B, et al. Transcriptome-wide mapping reveals widespread dynamic-regulated pseudouridylation of ncRNA and mRNA. Cell. 2014;159:148-162 pubmed publisher
    ..Our work identifies an enhanced, transcriptome-wide scope for pseudouridine and methods to dissect its underlying mechanisms and function. ..
  7. Dez C, Noaillac Depeyre J, Caizergues Ferrer M, Henry Y. Naf1p, an essential nucleoplasmic factor specifically required for accumulation of box H/ACA small nucleolar RNPs. Mol Cell Biol. 2002;22:7053-65 pubmed
    ..Naf1p is unlikely to be directly required for the synthesis of H/ACA snoRNP components. Naf1p could participate in H/ACA snoRNP assembly and/or transport. ..
  8. Koo B, Park C, Fernandez C, Chim N, Ding Y, Chanfreau G, et al. Structure of H/ACA RNP protein Nhp2p reveals cis/trans isomerization of a conserved proline at the RNA and Nop10 binding interface. J Mol Biol. 2011;411:927-42 pubmed publisher
    ..one of four essential protein components of eukaryotic H/ACA RNPs, forms a core trimer with the pseudouridylase Cbf5 and Nop10 that binds to H/ACA RNAs specifically...
  9. Jiang W, Lim M, Yoon H, Thorner J, Martin G, Carbon J. Overexpression of the yeast MCK1 protein kinase suppresses conditional mutations in centromere-binding protein genes CBF2 and CBF5. Mol Gen Genet. 1995;246:360-6 pubmed
    ..the temperature-sensitive phenotype of certain mutations in essential centromere binding protein genes CBF2 and CBF5. Since Mck1p is a known serine/threonine protein kinase, this suppression is postulated to be due to Mck1p-..

More Information


  1. Fortes P, Kufel J, Fornerod M, Polycarpou Schwarz M, Lafontaine D, Tollervey D, et al. Genetic and physical interactions involving the yeast nuclear cap-binding complex. Mol Cell Biol. 1999;19:6543-53 pubmed
    ..Mutants lacking yCBC were found to be defective in rRNA processing. Analysis of the yCBC deletion phenotype suggests that this is likely to be due to a defect in the splicing of a subset of ribosomal protein mRNA precursors. ..
  2. Jack K, Bellodi C, Landry D, Niederer R, Meskauskas A, Musalgaonkar S, et al. rRNA pseudouridylation defects affect ribosomal ligand binding and translational fidelity from yeast to human cells. Mol Cell. 2011;44:660-6 pubmed publisher
    ..from a yeast strain in which Cbf5p, the yeast homolog of DKC1, is catalytically impaired through a D95A mutation (cbf5-D95A)...
  3. Hertveldt K, Dechassa M, Robben J, Volckaert G. Identification of Gal80p-interacting proteins by Saccharomyces cerevisiae whole genome phage display. Gene. 2003;307:141-9 pubmed
    ..Interactions were confirmed by ELISA. These results demonstrate the accessibility of the S. cerevisiae genome to display technology for protein-protein interaction screening. ..
  4. Ma X, Yang C, Alexandrov A, Grayhack E, Behm Ansmant I, Yu Y. Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism. EMBO J. 2005;24:2403-13 pubmed
    ..Using the snR81-deletion strain, Nhp2p- or Cbf5p-conditional depletion strain, and a cbf5 mutation strain, we further demonstrated that the pseudouridylase activity is dependent on snR81 snoRNP in vivo...
  5. Godin K, Walbott H, Leulliot N, van Tilbeurgh H, Varani G. The box H/ACA snoRNP assembly factor Shq1p is a chaperone protein homologous to Hsp90 cochaperones that binds to the Cbf5p enzyme. J Mol Biol. 2009;390:231-44 pubmed publisher
    ..These results provide the first evidence of a specific biochemical activity for Shq1p and a direct link to the H/ACA snoRNP. ..
  6. Leulliot N, Godin K, Hoareau Aveilla C, Quevillon Cheruel S, Varani G, Henry Y, et al. The box H/ACA RNP assembly factor Naf1p contains a domain homologous to Gar1p mediating its interaction with Cbf5p. J Mol Biol. 2007;371:1338-53 pubmed
    ..The structural homology with Gar1 suggests that the function of Naf1 involves preventing non-cognate RNAs from being loaded during transport of the particle by inducing a non-productive conformation of Cbf5.
  7. Sydorskyy Y, Dilworth D, Halloran B, Yi E, Makhnevych T, Wozniak R, et al. Nop53p is a novel nucleolar 60S ribosomal subunit biogenesis protein. Biochem J. 2005;388:819-26 pubmed
  8. Koonin E. Pseudouridine synthases: four families of enzymes containing a putative uridine-binding motif also conserved in dUTPases and dCTP deaminases. Nucleic Acids Res. 1996;24:2411-5 pubmed
    ..These particular RNA modifications and the respective enzymes are likely to be essential for the functioning of any cell. ..
  9. Hoskins J, Butler J. RNA-based 5-fluorouracil toxicity requires the pseudouridylation activity of Cbf5p. Genetics. 2008;179:323-30 pubmed publisher
  10. Walbott H, Machado Pinilla R, Liger D, Blaud M, Rety S, Grozdanov P, et al. The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic. Genes Dev. 2011;25:2398-408 pubmed publisher
    ..We propose that Shq1p operates as a Cbf5p chaperone during RNP assembly by acting as an RNA placeholder, thereby preventing Cbf5p from nonspecific RNA binding before association with an H/ACA RNA and the other core RNP proteins. ..
  11. Li S, Duan J, Li D, Yang B, Dong M, Ye K. Reconstitution and structural analysis of the yeast box H/ACA RNA-guided pseudouridine synthase. Genes Dev. 2011;25:2409-21 pubmed publisher
    ..We reconstituted functional Saccharomyces cerevisiae H/ACA RNPs with recombinant proteins Cbf5, Nop10, Gar1, and Nhp2 and a two-hairpin H/ACA RNA; determined the crystal structure of a Cbf5, Nop10, and Gar1 ..
  12. Mouaikel J, Verheggen C, Bertrand E, Tazi J, Bordonné R. Hypermethylation of the cap structure of both yeast snRNAs and snoRNAs requires a conserved methyltransferase that is localized to the nucleolus. Mol Cell. 2002;9:891-901 pubmed
    ..Consistently, Tgs1p is also localized in the nucleolus. Our results suggest a trafficking pathway in which yeast snRNAs and snoRNAs cycle through the nucleolus to undergo m(7)G cap hypermethylation. ..
  13. Normand C, Capeyrou R, Quevillon Cheruel S, Mougin A, Henry Y, Caizergues Ferrer M. Analysis of the binding of the N-terminal conserved domain of yeast Cbf5p to a box H/ACA snoRNA. RNA. 2006;12:1868-82 pubmed
    ..In this study, we present the purification of a truncated derivative of yeast Cbf5p (Cbf5(Delta)p) that retains the highly conserved TRUB and PUA domains...
  14. Ballarino M, Morlando M, Pagano F, Fatica A, Bozzoni I. The cotranscriptional assembly of snoRNPs controls the biosynthesis of H/ACA snoRNAs in Saccharomyces cerevisiae. Mol Cell Biol. 2005;25:5396-403 pubmed
    ..All these data suggest that proper cotranscriptional snoRNP assembly controls 3'-end formation of snoRNAs and, consequently, the release of a functional particle. ..
  15. Henras A, Capeyrou R, Henry Y, Caizergues Ferrer M. Cbf5p, the putative pseudouridine synthase of H/ACA-type snoRNPs, can form a complex with Gar1p and Nop10p in absence of Nhp2p and box H/ACA snoRNAs. RNA. 2004;10:1704-12 pubmed
    ..We also show that the absence of any component necessary for assembly of box H/ACA snoRNPs inhibits accumulation of Cbf5p, Gar1p, or Nop10p, whereas Nhp2p levels are little affected. ..
  16. Fatica A, Dlakic M, Tollervey D. Naf1 p is a box H/ACA snoRNP assembly factor. RNA. 2002;8:1502-14 pubmed
    ..We propose that Naf1 p is recruited to the CTD of RNA polymerase II and binds to nascent box H/ACA snoRNAs promoting snoRNP assembly. ..
  17. Li S, Duan J, Li D, Ma S, Ye K. Structure of the Shq1-Cbf5-Nop10-Gar1 complex and implications for H/ACA RNP biogenesis and dyskeratosis congenita. EMBO J. 2011;30:5010-20 pubmed publisher
    ..We report the structure of the Shq1-specific domain alone and in complex with H/ACA RNP proteins Cbf5, Nop10 and Gar1...