Gene Symbol: CAK1
Description: cyclin-dependent protein kinase-activating kinase CAK1
Alias: CIV1, cyclin-dependent protein kinase-activating kinase CAK1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kaldis P, Sutton A, Solomon M. The Cdk-activating kinase (CAK) from budding yeast. Cell. 1996;86:553-64 pubmed
    ..coli, and is not a component of the basal transcription factor, TFIIH. A temperature-sensitive mutation in CAK1 confers a G2 delay accompanied by low Cdc28p protein kinase activity and shows genetic interactions with altered ..
  2. Thuret J, Valay J, Faye G, Mann C. Civ1 (CAK in vivo), a novel Cdk-activating kinase. Cell. 1996;86:565-76 pubmed
    ..We report the identification of Civ1 (CAK in vivo), a novel CAK activity in S. cerevisiae...
  3. Kitazono A, Kron S. An essential function of yeast cyclin-dependent kinase Cdc28 maintains chromosome stability. J Biol Chem. 2002;277:48627-34 pubmed
    ..A dosage suppression screen identified Cak1, a kinase known to phosphorylate and activate Cdc28, as a specific high copy suppressor of cdc28(CST) temperature ..
  4. Kaldis P, Pitluk Z, Bany I, Enke D, Wagner M, Winter E, et al. Localization and regulation of the cdk-activating kinase (Cak1p) from budding yeast. J Cell Sci. 1998;111 ( Pt 24):3585-96 pubmed
    ..The localization and regulation of Cak1p are in contrast to those of the known vertebrate CAK, p40(MO15). ..
  5. Mort Bontemps Soret M, Facca C, Faye G. Physical interaction of Cdc28 with Cdc37 in Saccharomyces cerevisiae. Mol Genet Genomics. 2002;267:447-58 pubmed
    ..In a genetic screen, four protein kinases were identified as interacting with Cdc37 - Cdc5, Cdc7, Cdc15 and Cak1. This result underlines the importance of Cdc37 for the folding of protein kinases...
  6. Davenport K, Williams K, Ullmann B, Gustin M. Activation of the Saccharomyces cerevisiae filamentation/invasion pathway by osmotic stress in high-osmolarity glycogen pathway mutants. Genetics. 1999;153:1091-103 pubmed
    ..Three high-copy suppressors of pbs2-3 osmosensitivity were identified: MSG5, CAK1, and TRX1...
  7. Millson S, van Oosten Hawle P, Alkuriji M, Truman A, Siderius M, Piper P. Cdc37 engages in stable, S14A mutation-reinforced association with the most atypical member of the yeast kinome, Cdk-activating kinase (Cak1). Cell Stress Chaperones. 2014;19:695-703 pubmed publisher
    ..association with the most atypical member of the yeast kinome, cyclin-dependent kinase (Cdk1)-activating kinase (Cak1)...
  8. Brands A, Skibbens R. Sister chromatid cohesion role for CDC28-CDK in Saccharomyces cerevisiae. Genetics. 2008;180:7-16 pubmed publisher
    ..These and other results reveal that Ctf7p regulation is more complicated than previously envisioned and suggest that CDK acts in sister chromatid cohesion parallel to Ctf7p reactions. ..
  9. Schindler K, Benjamin K, Martin A, Boglioli A, Herskowitz I, Winter E. The Cdk-activating kinase Cak1p promotes meiotic S phase through Ime2p. Mol Cell Biol. 2003;23:8718-28 pubmed
    b>CAK1 encodes an essential protein kinase in Saccharomyces cerevisiae that is required for activation of the Cdc28p Cdk. CAK1 also has several CDC28-independent functions that are unique to meiosis...

More Information


  1. Cross F, Levine K. Genetic analysis of the relationship between activation loop phosphorylation and cyclin binding in the activation of the Saccharomyces cerevisiae Cdc28p cyclin-dependent kinase. Genetics. 2000;154:1549-59 pubmed
    ..This conclusion was supported by analysis of suppressors of a mutation in the Cdk phosphothreonine-binding pocket created by cyclin binding. ..
  2. Schaber M, Lindgren A, Schindler K, Bungard D, Kaldis P, Winter E. CAK1 promotes meiosis and spore formation in Saccharomyces cerevisiae in a CDC28-independent fashion. Mol Cell Biol. 2002;22:57-68 pubmed
    b>CAK1 encodes a protein kinase in Saccharomyces cerevisiae whose sole essential mitotic role is to activate the Cdc28p cyclin-dependent kinase by phosphorylation of threonine-169 in its activation loop...
  3. Duttler S, Pechmann S, Frydman J. Principles of cotranslational ubiquitination and quality control at the ribosome. Mol Cell. 2013;50:379-93 pubmed publisher
    ..We find that quality control at the ribosome is achieved through a tiered system wherein nascent polypeptides have a chance to fold before becoming accessible to ubiquitination. ..
  4. Yao S, Prelich G. Activation of the Bur1-Bur2 cyclin-dependent kinase complex by Cak1. Mol Cell Biol. 2002;22:6750-8 pubmed
    ..we searched for high-copy-number suppressors of a bur1 temperature-sensitive mutation, identifying a single gene, CAK1. Cak1 is known to activate two other Cdks in yeast by phosphorylating a threonine within their conserved T-loop ..
  5. Chun K, Goebl M. Mutational analysis of Cak1p, an essential protein kinase that regulates cell cycle progression. Mol Gen Genet. 1997;256:365-75 pubmed
    ..Here we describe mutations in another gene, CAK1, that lead to a similar arrest...
  6. Ross K, Kaldis P, Solomon M. Activating phosphorylation of the Saccharomyces cerevisiae cyclin-dependent kinase, cdc28p, precedes cyclin binding. Mol Biol Cell. 2000;11:1597-609 pubmed
    ..We propose that Cdc28p is normally phosphorylated by Cak1p before it binds cyclin. This activation pathway contrasts with that in higher eukaryotes, in which cyclin binding appears to precede activating phosphorylation. ..
  7. Bieganowski P, Garrison P, Hodawadekar S, Faye G, Barnes L, Brenner C. Adenosine monophosphoramidase activity of Hint and Hnt1 supports function of Kin28, Ccl1, and Tfb3. J Biol Chem. 2002;277:10852-60 pubmed publisher
    ..and Kin28, which constitute the TFIIK kinase subcomplex of general transcription factor TFIIH and to mutations in Cak1, which phosphorylates Kin28...
  8. Kim S, Gadiparthi K, Kron S, Kitazono A. A phosphorylation-independent role for the yeast cyclin-dependent kinase activating kinase Cak1. Gene. 2009;447:97-105 pubmed publisher
    ..Besides cyclin binding, Cdc28 requires phosphorylation by the Cak1 kinase to achieve full activity...
  9. Sutton A, Freiman R. The Cak1p protein kinase is required at G1/S and G2/M in the budding yeast cell cycle. Genetics. 1997;147:57-71 pubmed
    The CAK1 gene encodes the major CDK-activating kinase (CAK) in budding yeast and is required for activation of Cdc28p for cell cycle progression from G2 to M phase...
  10. Ceccarelli E, Mann C. A Cdc28 mutant uncouples G1 cyclin phosphorylation and ubiquitination from G1 cyclin proteolysis. J Biol Chem. 2001;276:41725-32 pubmed
    ..In this study, we identified a cak1 allele in a genetic screen for mutants that stabilize the yeast G(1) cyclins...
  11. Kimmelman J, Kaldis P, Hengartner C, Laff G, Koh S, Young R, et al. Activating phosphorylation of the Kin28p subunit of yeast TFIIH by Cak1p. Mol Cell Biol. 1999;19:4774-87 pubmed
    ..These findings indicate that although MO15 and Cak1p constitute different forms of CAK, both control the cell cycle and the phosphorylation of the C-terminal domain of the large subunit of RNA polymerase II by TFIIH. ..
  12. Cheng A, Ross K, Kaldis P, Solomon M. Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases. Genes Dev. 1999;13:2946-57 pubmed
    ..Overexpression of PTC2 or PTC3 results in synthetic lethality in strains temperature-sensitive for yeast CAK1, and disruptions of PTC2 and PTC3 suppress the growth defect of a cak1 mutant...
  13. Cross F, Levine K. Molecular evolution allows bypass of the requirement for activation loop phosphorylation of the Cdc28 cyclin-dependent kinase. Mol Cell Biol. 1998;18:2923-31 pubmed
    ..Some growth defects remain in suppressed cak1 cdc28 strains carrying the mutant CDC28 genes, consistent with additional nonessential roles for CAK1.
  14. Wagner M, Pierce M, Winter E. The CDK-activating kinase CAK1 can dosage suppress sporulation defects of smk1 MAP kinase mutants and is required for spore wall morphogenesis in Saccharomyces cerevisiae. EMBO J. 1997;16:1305-17 pubmed
    ..b>CAK1 encodes the major CAK activity in yeast and is required for cell cycle progression...
  15. Devos M, Mommaerts E, Migeot V, van Bakel H, Hermand D. Fission yeast Cdk7 controls gene expression through both its CAK and C-terminal domain kinase activities. Mol Cell Biol. 2015;35:1480-90 pubmed publisher
    ..Further analyses revealed that Csk1 lacks a 20-amino-acid sequence required for its budding yeast counterpart, Cak1, to bind Cdc2...
  16. Espinoza F, Farrell A, Erdjument Bromage H, Tempst P, Morgan D. A cyclin-dependent kinase-activating kinase (CAK) in budding yeast unrelated to vertebrate CAK. Science. 1996;273:1714-7 pubmed
    ..CAK from the budding yeast Saccharomyces cerevisiae was identified as an unusual 44-kilodalton protein kinase, Cak1, that is only distantly related to CDKs...
  17. Schmidt M, Drgon T, Bowers B, Cabib E. Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3. FEMS Yeast Res. 2008;8:362-73 pubmed publisher
    ..wall defects in Saccharomyces cerevisiae, we isolated a strain carrying a mutation in the Cdc28-activating kinase CAK1. The cak1P212S mutant cells exhibit multiple, elongated and branched buds, beta(1,3)glucan-poor regions of the cell ..
  18. Ostapenko D, Solomon M. Phosphorylation by Cak1 regulates the C-terminal domain kinase Ctk1 in Saccharomyces cerevisiae. Mol Cell Biol. 2005;25:3906-13 pubmed
    ..As with other yeast CDKs undergoing T-loop phosphorylation, Ctk1 phosphorylation on Thr-338 was dependent on the Cak1 protein kinase. Ctk1 isolated from cak1Delta cells was unphosphorylated and exhibited low protein kinase activity...
  19. Whinston E, Omerza G, Singh A, Tio C, Winter E. Activation of the Smk1 mitogen-activated protein kinase by developmentally regulated autophosphorylation. Mol Cell Biol. 2013;33:688-700 pubmed publisher
    ..the T residue in Smk1's activation motif is phosphorylated by the cyclin-dependent kinase (CDK)-activating kinase, Cak1. The Y residue is autophosphorylated in an independent intramolecular reaction that requires the meiosis-specific ..
  20. Ganem C, Miled C, Facca C, Valay J, Labesse G, Ben Hassine S, et al. Kinase Cak1 functionally interacts with the PAF1 complex and phosphatase Ssu72 via kinases Ctk1 and Bur1. Mol Genet Genomics. 2006;275:136-47 pubmed
    Protein kinases orthologous with Cak1 of Saccharomyces cerevisiae (ScCak1) appear specific to ascomycetes...
  21. Espinoza F, Farrell A, Nourse J, Chamberlin H, Gileadi O, Morgan D. Cak1 is required for Kin28 phosphorylation and activation in vivo. Mol Cell Biol. 1998;18:6365-73 pubmed
    ..In the budding yeast, Saccharomyces cerevisiae, the major CAK is a 44-kDa protein kinase known as Cak1. Cak1 is required for the phosphorylation and activation of Cdc28, a major CDK involved in cell cycle control...