Gene Symbol: BUD4
Description: Bud4p
Alias: Bud4p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Sanders S, Herskowitz I. The BUD4 protein of yeast, required for axial budding, is localized to the mother/BUD neck in a cell cycle-dependent manner. J Cell Biol. 1996;134:413-27 pubmed
    ..Misumi. 1994. Nature (Lond.). 372:567-570). We cloned and sequenced BUD4, which codes for a large, novel protein (Bud4p) with a potential GTP-binding motif. Bud4p is expressed and localized to the mother/bud neck in all cell types...
  2. Madden K, Snyder M. Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol. 1998;52:687-744 pubmed
    ..Since many of the components important for polarized cell growth are conserved in other organisms, the basic mechanisms mediating polarized cell growth are likely to be universal among eukaryotes. ..
  3. Gao X, Sperber L, Kane S, Tong Z, Tong A, Boone C, et al. Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle. Mol Biol Cell. 2007;18:2542-60 pubmed
    ..is not known when and how Axl2p functions together with other components of the axial landmark, such as Bud3p and Bud4p, to direct axial budding...
  4. Kang P, Angerman E, Jung C, Park H. Bud4 mediates the cell-type-specific assembly of the axial landmark in budding yeast. J Cell Sci. 2012;125:3840-9 pubmed publisher
    ..a and ? cells bud in the axial budding pattern, which depends on a transient marker and requires proteins Bud3, Bud4, Axl1 and Axl2...
  5. Kang P, Hood Degrenier J, Park H. Coupling of septins to the axial landmark by Bud4 in budding yeast. J Cell Sci. 2013;126:1218-26 pubmed publisher
    ..Haploid a and ? cells bud in the axial budding pattern, which requires assembly of a landmark that includes the Bud4 protein. To understand how an axial bud site is established, we performed a structure-function analysis of Bud4...
  6. Lord M, Inose F, Hiroko T, Hata T, Fujita A, Chant J. Subcellular localization of Axl1, the cell type-specific regulator of polarity. Curr Biol. 2002;12:1347-52 pubmed
    ..Axl1 is absent from diploids. Axl1 colocalizes with Bud3, Bud4, and Bud10, components of the axial landmark structure...
  7. Voth W, Olsen A, Sbia M, Freedman K, Stillman D. ACE2, CBK1, and BUD4 in budding and cell separation. Eukaryot Cell. 2005;4:1018-28 pubmed
    ..We show that wild-type W303 strains have a bud4 mutation and that combining bud4 with either ace2 or cbk1 in haploids results in altered colony morphology...
  8. Gimeno C, Fink G. Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. Mol Cell Biol. 1994;14:2100-12 pubmed
    ..pseudohyphal growth in a haploid strain that has the diploid polar budding pattern because of a mutation in the BUD4 gene. In addition, wild-type diploid strains lacking PHD1 undergo pseudohyphal growth when starved for nitrogen...
  9. Yang S, Ayscough K, Drubin D. A role for the actin cytoskeleton of Saccharomyces cerevisiae in bipolar bud-site selection. J Cell Biol. 1997;136:111-23 pubmed

More Information


  1. Cullen P, Sprague G. The roles of bud-site-selection proteins during haploid invasive growth in yeast. Mol Biol Cell. 2002;13:2990-3004 pubmed
    ..Three of the four known proteins required for axial budding, Bud3p, Bud4p, and Axl2p, were expressed and localized appropriately in glucose-limiting conditions...
  2. Osman M, Konopka J, Cerione R. Iqg1p links spatial and secretion landmarks to polarity and cytokinesis. J Cell Biol. 2002;159:601-11 pubmed
    ..how Iqg1p influences these processes, we performed a two-hybrid screen and identified the spatial landmark Bud4p as a binding partner...
  3. Eluère R, Varlet I, Bernadac A, Simon M. Cdk and the anillin homolog Bud4 define a new pathway regulating septin organization in yeast. Cell Cycle. 2012;11:151-8 pubmed publisher
    ..In this study, we report a role of the anillin-like protein Bud4 in the formation and the disassembly of the double ring structure formed by the septins at the time of cytokinesis...
  4. Meitinger F, Richter H, Heisel S, Hub B, Seufert W, Pereira G. A safeguard mechanism regulates Rho GTPases to coordinate cytokinesis with the establishment of cell polarity. PLoS Biol. 2013;11:e1001495 pubmed publisher
    ..Our findings provide unexpected insights into the temporal control of cytokinesis and describe the importance of a Gps1-dependent mechanism for highly accurate polarity switching between two closely connected locations. ..
  5. Kang P, Lee M, Park H. Bud3 activates Cdc42 to establish a proper growth site in budding yeast. J Cell Biol. 2014;206:19-28 pubmed publisher
    ..Biphasic activation of Cdc42 or Rac GTPases may be a general mechanism for spatial cue-directed cell polarization in eukaryotes...
  6. Wu H, Guo J, Zhou Y, Gao X. The anillin-related region of Bud4 is the major functional determinant for Bud4's function in septin organization during bud growth and axial bud site selection in budding yeast. Eukaryot Cell. 2015;14:241-51 pubmed publisher
    The anillin-related protein Bud4 of Saccharomyces cerevisiae is required for axial bud site selection by linking the axial landmark to the septins, which localize at the mother bud neck...
  7. Finnigan G, Duvalyan A, Liao E, Sargsyan A, Thorner J. Detection of protein-protein interactions at the septin collar in Saccharomyces cerevisiae using a tripartite split-GFP system. Mol Biol Cell. 2016;27:2708-25 pubmed publisher
    ..additional new information by examining whether three other proteins reported to localize to the bud neck (Nis1, Bud4, and Hof1) are able to interact physically with any of the subunits in the septin collar and, if so, with which ..