Gene Symbol: BUB2
Description: Bub2p
Alias: PAC7, Bub2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. D Aquino K, Monje Casas F, Paulson J, Reiser V, Charles G, Lai L, et al. The protein kinase Kin4 inhibits exit from mitosis in response to spindle position defects. Mol Cell. 2005;19:223-34 pubmed
  2. Visintin R, Stegmeier F, Amon A. The role of the polo kinase Cdc5 in controlling Cdc14 localization. Mol Biol Cell. 2003;14:4486-98 pubmed
    ..Cdc5 promotes Cdc14 phosphorylation and, by stimulating the MEN, Cfi1/Net1 phosphorylation. Furthermore, we suggest that Cdc14 release from the nucleolus only occurs when Cdc14 and Cfi1/Net1 are both phosphorylated. ..
  3. Ro H, Song S, Lee K. Bfa1 can regulate Tem1 function independently of Bub2 in the mitotic exit network of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2002;99:5436-41 pubmed
    ..body of evidence suggests that the Tem1 GTPase plays a critical role in mediating this pathway and that Bfa1 and Bub2 constitute a two-component GTPase-activating protein to negatively regulate Tem1...
  4. Li R. Bifurcation of the mitotic checkpoint pathway in budding yeast. Proc Natl Acad Sci U S A. 1999;96:4989-94 pubmed
    ..interaction of BFA1 with known mitotic checkpoint genes suggest that Bfa1 functions in the same pathway with Bub2 but not with Mad1 or Mad2...
  5. Lee S, Jensen S, Frenz L, Johnson A, Fesquet D, Johnston L. The Bub2-dependent mitotic pathway in yeast acts every cell cycle and regulates cytokinesis. J Cell Sci. 2001;114:2345-54 pubmed
    ..Recently, we and others identified a novel Bub2-dependent branch to this checkpoint that blocks mitotic exit...
  6. Maekawa H, Priest C, Lechner J, Pereira G, Schiebel E. The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal. J Cell Biol. 2007;179:423-36 pubmed
    ..Delay is achieved by maintaining the activity of the Bfa1-Bub2 guanosine triphosphatase-activating protein complex, an inhibitor of mitotic exit...
  7. Ross K, COHEN FIX O. The role of Cdh1p in maintaining genomic stability in budding yeast. Genetics. 2003;165:489-503 pubmed
    ..We propose that the failure to degrade cyclins at the end of mitosis leaves cdh1delta mutant strains with abnormal Cdc28p/Clb activity that interferes with proper chromosome segregation. ..
  8. Rossio V, Galati E, Ferrari M, Pellicioli A, Sutani T, Shirahige K, et al. The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase. J Cell Biol. 2010;191:981-97 pubmed publisher
    ..Our data suggest that fine-tuning regulators of mitotic exit have important functions during mitotic progression in cells treated with microtubule poisons and might be promising targets for cancer treatment. ..
  9. Hu F, Wang Y, Liu D, Li Y, Qin J, Elledge S. Regulation of the Bub2/Bfa1 GAP complex by Cdc5 and cell cycle checkpoints. Cell. 2001;107:655-65 pubmed
    ..During most of the cell cycle, Tem1 function is antagonized by a GTPase-activating protein complex, Bfa1/Bub2. How the Bfa1/Bub2 complex is regulated is not well understood...

More Information


  1. Daum J, Gomez Ospina N, Winey M, Burke D. The spindle checkpoint of Saccharomyces cerevisiae responds to separable microtubule-dependent events. Curr Biol. 2000;10:1375-8 pubmed
    ..We localized a Bub2p-GFP fusion protein to the cytoplasmic side of the spindle pole body and used a kar9 mutant to show that cells with ..
  2. Fraschini R, Formenti E, Lucchini G, Piatti S. Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. J Cell Biol. 1999;145:979-91 pubmed
    ..We investigated the role of Bub2 in budding yeast mitotic checkpoint...
  3. Snead J, Sullivan M, Lowery D, Cohen M, Zhang C, Randle D, et al. A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration. Chem Biol. 2007;14:1261-72 pubmed
    ..Finally, an examination of Cdc5 binding by SPB-localized proteins expanded our knowledge of Cdc5 function at the SPB. ..
  4. Caydasi A, Kurtulmus B, Orrico M, Hofmann A, Ibrahim B, Pereira G. Elm1 kinase activates the spindle position checkpoint kinase Kin4. J Cell Biol. 2010;190:975-89 pubmed publisher
    ..These findings establish a novel function for Elm1 in the coordination of spindle positioning with cell cycle progression via its control of Kin4. ..
  5. Geymonat M, Spanos A, Smith S, Wheatley E, Rittinger K, Johnston L, et al. Control of mitotic exit in budding yeast. In vitro regulation of Tem1 GTPase by Bub2 and Bfa1. J Biol Chem. 2002;277:28439-45 pubmed
    ..that mitotic exit network activity is determined by a GTP-binding protein, Tem1, and its putative regulators, Bub2, Bfa1, and Lte1. Here we describe the purification and in vitro activities of Tem1, Bub2, and Bfa1...
  6. Höfken T, Schiebel E. A role for cell polarity proteins in mitotic exit. EMBO J. 2002;21:4851-62 pubmed
    ..Finally, the cell polarity proteins Kel1 and Kel2 are present in complexes with both Lte1 and Tem1, and negatively regulate mitotic exit. ..
  7. Fraschini R, D Ambrosio C, Venturetti M, Lucchini G, Piatti S. Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit. J Cell Biol. 2006;172:335-46 pubmed
    ..spindles and prevents mitotic exit by inhibiting the G protein Tem1 through the GTPase-activating protein (GAP) Bub2/Bfa1...
  8. Wang Y, Hu F, Elledge S. The Bfa1/Bub2 GAP complex comprises a universal checkpoint required to prevent mitotic exit. Curr Biol. 2000;10:1379-82 pubmed
    ..In the spindle checkpoint pathway, this is accomplished through Bfa1/Bub2, a heteromeric GTPase-activating protein (GAP) that inhibits Clb degradation by keeping the G protein Tem1 inactive ..
  9. Gruneberg U, Campbell K, Simpson C, Grindlay J, Schiebel E. Nud1p links astral microtubule organization and the control of exit from mitosis. EMBO J. 2000;19:6475-88 pubmed
    ..is distinct from its role in cell-cycle control: Nud1p binds the spindle checkpoint control proteins Bfa1p and Bub2p to the SPB, and is part of the mitotic exit network (MEN) in which it functions upstream of CDC15 but downstream ..
  10. Alexandru G, Zachariae W, Schleiffer A, Nasmyth K. Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage. EMBO J. 1999;18:2707-21 pubmed
    ..Blocking APCCdh1-mediated Clb2 proteolysis and chromosome re-duplication does not require Mad2 but a different protein, Bub2. Our data imply that Mad1, Mad2, Mad3 and Bub1 regulate APCCdc20, whereas Bub2 regulates APCCdh1.
  11. Kim J, Jeong J, Song K. The C-terminus of Bfa1p in budding yeast is essential to induce mitotic arrest in response to diverse checkpoint-activating signals. Genes Cells. 2004;9:399-418 pubmed
    ..Bfa1p in association with Bub2p negatively regulates Tem1p in response to spindle damage, spindle misorientation, and DNA damage, resulting in ..
  12. Jensen S, Geymonat M, Johnson A, Segal M, Johnston L. Spatial regulation of the guanine nucleotide exchange factor Lte1 in Saccharomyces cerevisiae. J Cell Sci. 2002;115:4977-91 pubmed
    ..Tem1 is regulated by Bub2/Bfa1, a two-component GTPase-activating protein (GAP), and by Lte1, a putative guanine nucleotide exchange factor...
  13. Kim J, Luo G, Bahk Y, Song K. Cdc5-dependent asymmetric localization of bfa1 fine-tunes timely mitotic exit. PLoS Genet. 2012;8:e1002450 pubmed publisher
    ..the mitotic exit network (MEN) is Tem1, a GTPase, which is inhibited by the GTPase-activating protein (GAP), Bfa1/Bub2. Asymmetric Bfa1 localization to the bud-directed spindle pole body (SPB) during metaphase also controls mitotic ..
  14. Miller C, Gabrielse C, Chen Y, Weinreich M. Cdc7p-Dbf4p regulates mitotic exit by inhibiting Polo kinase. PLoS Genet. 2009;5:e1000498 pubmed publisher
    ..Therefore, Cdc7p-Dbf4p prevents inappropriate exit from mitosis by inhibiting Polo kinase and functions in the spindle position checkpoint. ..
  15. Kim J, Jang S, Song K. Different levels of Bfa1/Bub2 GAP activity are required to prevent mitotic exit of budding yeast depending on the type of perturbations. Mol Biol Cell. 2008;19:4328-40 pubmed publisher
    In budding yeast, Tem1 is a key regulator of mitotic exit. Bfa1/Bub2 stimulates Tem1 GTPase activity as a GTPase-activating protein (GAP). Lte1 possesses a guanine-nucleotide exchange factor (GEF) domain likely for Tem1...
  16. Myung K, Smith S, Kolodner R. Mitotic checkpoint function in the formation of gross chromosomal rearrangements in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2004;101:15980-5 pubmed
    ..These data suggest that functional mitotic checkpoints can play a role in the formation of genome rearrangements. ..
  17. Yoshida S, Asakawa K, Toh e A. Mitotic exit network controls the localization of Cdc14 to the spindle pole body in Saccharomyces cerevisiae. Curr Biol. 2002;12:944-50 pubmed
    ..The Cdc14 liberation from the nucleolus was inhibited by the Mad2 checkpoint and by the Bub2 checkpoint in a different manner when microtubule organization was disrupted...
  18. Fesquet D, Fitzpatrick P, Johnson A, Kramer K, Toyn J, Johnston L. A Bub2p-dependent spindle checkpoint pathway regulates the Dbf2p kinase in budding yeast. EMBO J. 1999;18:2424-34 pubmed
    ..It is controlled specifically by Bub2p, one of the checkpoint components that is conserved in fission yeast and higher eukaroytic cells...
  19. König C, Maekawa H, Schiebel E. Mutual regulation of cyclin-dependent kinase and the mitotic exit network. J Cell Biol. 2010;188:351-68 pubmed publisher
    ..The inhibitory Bfa1-Bub2 GTPase-activating protein (GAP) only associates with the daughter SPB (dSPB), raising the question as to how the ..
  20. Pereira G, Schiebel E. Kin4 kinase delays mitotic exit in response to spindle alignment defects. Mol Cell. 2005;19:209-21 pubmed
    ..One component of the SPOC is the Bub2-Bfa1 complex, an inhibitor of the mitotic exit network (MEN)...
  21. Pereira G, Manson C, Grindlay J, Schiebel E. Regulation of the Bfa1p-Bub2p complex at spindle pole bodies by the cell cycle phosphatase Cdc14p. J Cell Biol. 2002;157:367-79 pubmed
    ..Upon release, Cdc14p binds to the spindle pole body (SPB) via association with the Bfa1p-Bub2p GTPase activating protein complex, which is known to regulate the activity of the G protein Tem1p...
  22. Stegmeier F, Visintin R, Amon A. Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase. Cell. 2002;108:207-20 pubmed
    ..We propose that one function of Cdc14 released by the FEAR network is to stimulate MEN activity. ..
  23. Tavormina P, Burke D. Cell cycle arrest in cdc20 mutants of Saccharomyces cerevisiae is independent of Ndc10p and kinetochore function but requires a subset of spindle checkpoint genes. Genetics. 1998;148:1701-13 pubmed
    ..We show that the lesion recognized by Bub2p is not excess microtubules, and the cdc20 arrest is independent of kinetochore function...
  24. Fraschini R, Bilotta D, Lucchini G, Piatti S. Functional characterization of Dma1 and Dma2, the budding yeast homologues of Schizosaccharomyces pombe Dma1 and human Chfr. Mol Biol Cell. 2004;15:3796-810 pubmed
    ..The latter defects can be rescued by either eliminating the spindle position checkpoint protein Bub2 or overproducing its target, Tem1, both leading to MEN hyperactivation...
  25. Krishnan R, Pangilinan F, Lee C, Spencer F. Saccharomyces cerevisiae BUB2 prevents mitotic exit in response to both spindle and kinetochore damage. Genetics. 2000;156:489-500 pubmed
    ..In the presence of either damage, bub2 cells initiate a preanaphase delay but do not maintain it...
  26. Pereira G, Höfken T, Grindlay J, Manson C, Schiebel E. The Bub2p spindle checkpoint links nuclear migration with mitotic exit. Mol Cell. 2000;6:1-10 pubmed
    Bfa1p and Bub2p are spindle checkpoint proteins that likely have GTPase activation activity and are associated with the budding yeast spindle pole body (SPB)...
  27. Chan L, Amon A. The protein phosphatase 2A functions in the spindle position checkpoint by regulating the checkpoint kinase Kin4. Genes Dev. 2009;23:1639-49 pubmed publisher
  28. Chai C, Teh E, Yeong F. Unrestrained spindle elongation during recovery from spindle checkpoint activation in cdc15-2 cells results in mis-segregation of chromosomes. Mol Biol Cell. 2010;21:2384-98 pubmed publisher
    ..Our data indicate that a slower rate of transition through the cell division cycle can result in an inadequate level of Pds1p accumulation that can compromise recovery from spindle assembly checkpoint activation. ..
  29. Karumbati A, Wilson T. Abrogation of the Chk1-Pds1 checkpoint leads to tolerance of persistent single-strand breaks in Saccharomyces cerevisiae. Genetics. 2005;169:1833-44 pubmed
    ..We propose a model in which recombinational repair during S phase coupled with failure of the metaphase-anaphase checkpoint allows for tolerance of persistent single-strand breaks at the expense of genome stability. ..
  30. Geiser J, Schott E, Kingsbury T, Cole N, Totis L, Bhattacharyya G, et al. Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol Biol Cell. 1997;8:1035-50 pubmed
    ..These findings illustrate the complex cellular interactions exhibited by Cin8p, a member of a conserved spindle motor family. ..
  31. Kemmler S, Stach M, Knapp M, Ortiz J, Pfannstiel J, Ruppert T, et al. Mimicking Ndc80 phosphorylation triggers spindle assembly checkpoint signalling. EMBO J. 2009;28:1099-110 pubmed publisher
    ..Therefore, we conclude that a putative Mps1-dependent phosphorylation of Ndc80 is important for SAC activation at kinetochores. ..
  32. DeLillo N, Romero C, Lin H, Vancura A. Genetic evidence for a role of phospholipase C at the budding yeast kinetochore. Mol Genet Genomics. 2003;269:261-70 pubmed
    ..These results are consistent with the view that Plc1p affects kinetochore function, possibly by modulating the structure of centromeric chromatin. ..
  33. Valerio Santiago M, de Los Santos Velázquez A, Monje Casas F. Inhibition of the mitotic exit network in response to damaged telomeres. PLoS Genet. 2013;9:e1003859 pubmed publisher
    ..However, it is also necessary to restrain exit from mitosis by maintaining Bfa1-Bub2, the inhibitor of the Mitotic Exit Network (MEN), in an active state...
  34. Park S, Cable A, Blair J, Stockstill K, Shannnon K. Bub2 regulation of cytokinesis and septation in budding yeast. BMC Cell Biol. 2009;10:43 pubmed publisher
    ..b>Bub2 and its binding partner Bfa1 act as a GTPase activating protein (GAP) to negatively regulate the MEN GTPase Tem1...
  35. Farr K, Hoyt M. Bub1p kinase activates the Saccharomyces cerevisiae spindle assembly checkpoint. Mol Cell Biol. 1998;18:2738-47 pubmed
    ..overexpressing MPS1, the BUB1-5 delay was dependent upon the functions of the other checkpoint genes, including BUB2 and BUB3 and MAD1, MAD2, and MAD3...
  36. Hwang H, Song K. IBD2 encodes a novel component of the Bub2p-dependent spindle checkpoint in the budding yeast Saccharomyces cerevisiae. Genetics. 2002;161:595-609 pubmed
    ..Bfa1p is a spindle checkpoint regulator of budding yeast in the Bub2p checkpoint pathway for proper mitotic exit...
  37. Suzuki M, Igarashi R, Sekiya M, Utsugi T, Morishita S, Yukawa M, et al. Dynactin is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae. Nat Cell Biol. 2004;6:861-71 pubmed
    ..Components of the dynactin complex are required to achieve the G2 arrest whilst keeping cells highly viable. Thus, the dynactin complex has a function in a checkpoint that monitors cell wall synthesis. ..
  38. Hanlon S, Norris D, Vershon A. Depletion of H2A-H2B dimers in Saccharomyces cerevisiae triggers meiotic arrest by reducing IME1 expression and activating the BUB2-dependent branch of the spindle checkpoint. Genetics. 2003;164:1333-44 pubmed
    ..Additionally, deletions of BUB2 or BFA1, components of one branch of the spindle checkpoint pathway, bypass the meiotic arrest...
  39. Chan L, Amon A. Spindle position is coordinated with cell-cycle progression through establishment of mitotic exit-activating and -inhibitory zones. Mol Cell. 2010;39:444-54 pubmed publisher
    ..In this manner, spatial information is sensed and translated into a chemical signal. ..
  40. Moore J, Chudalayandi P, Heil Chapdelaine R, Cooper J. The spindle position checkpoint is coordinated by the Elm1 kinase. J Cell Biol. 2010;191:493-503 pubmed publisher
    ..Furthermore, we show that Elm1 promotes the activity of the checkpoint kinase Kin4. These findings reveal a novel function for Elm1 in the SPC and suggest how checkpoint activity may be linked to cellular organization. ..
  41. Machu C, Eluère R, Signon L, Simon M, de La Roche Saint André C, Bailly E. Spatially distinct functions of Clb2 in the DNA damage response. Cell Cycle. 2014;13:383-98 pubmed publisher
    ..Our study also unveils an essential role of nuclear Clb2 in both survival and adaptation to the DNA damage checkpoint, illustrating a spatially distinct dual function of this mitotic cyclin in the response to DNA damage. ..
  42. García Rodríguez L, Crider D, Gay A, Salanueva I, Boldogh I, Pon L. Mitochondrial inheritance is required for MEN-regulated cytokinesis in budding yeast. Curr Biol. 2009;19:1730-5 pubmed publisher
    ..Finally, hyperactivation of the MEN by deletion of BUB2 restores defects in cytokinesis in mdm10Delta and mmr1Delta gem1Delta cells and reduces the fidelity of ..
  43. Neff M, Burke D. A delay in the Saccharomyces cerevisiae cell cycle that is induced by a dicentric chromosome and dependent upon mitotic checkpoints. Mol Cell Biol. 1992;12:3857-64 pubmed
    ..The rate of dicentric chromosome loss, however, was elevated in the rad9 mutant. A mutation in BUB2, a gene required for arrest of cell division in response to loss of microtubule function, diminished the delay...
  44. Scarfone I, Venturetti M, Hotz M, Lengefeld J, Barral Y, Piatti S. Asymmetry of the budding yeast Tem1 GTPase at spindle poles is required for spindle positioning but not for mitotic exit. PLoS Genet. 2015;11:e1004938 pubmed publisher
    ..SPOC relies on the heterodimeric GTPase-activating protein Bub2/Bfa1 that inhibits the small GTPase Tem1, in turn essential for activating the mitotic exit network (MEN) kinase ..
  45. Spang A, Geissler S, Grein K, Schiebel E. gamma-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation. J Cell Biol. 1996;134:429-41 pubmed
    ..In addition, cell cycle arrest and survival of tub4-1 cells is dependent on the mitotic checkpoint control gene BUB2 (Hoyt, M.A., L. Totis, B.T. Roberts. 1991. Cell. 66:507-517), one of the cell's monitors of spindle integrity.
  46. Harper N, Al Greene N, Basrai M, Belanger K. Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae. Curr Genet. 2008;53:95-105 pubmed
    ..Our results suggest a novel functional connection between Nup1 and proteins comprising both the spindle pole body and early mitotic exit network. ..
  47. Wang Y, Ng T. Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiae. Mol Biol Cell. 2006;17:80-9 pubmed
    ..All the evidence suggests the negative role of PP2A in mitotic exit. Finally, our genetic and biochemical data suggest that PP2A regulates the phosphorylation of Tem1, which acts at the very top of MEN pathway. ..
  48. Bogomolnaya L, Pathak R, Guo J, Cham R, Aramayo R, Polymenis M. Hym1p affects cell cycle progression in Saccharomyces cerevisiae. Curr Genet. 2004;46:183-92 pubmed
    ..Our overall data suggest that Hym1p has a regulatory role in cell cycle progression. ..
  49. Grandin N, Charbonneau M. Budding yeast 14-3-3 proteins contribute to the robustness of the DNA damage and spindle checkpoints. Cell Cycle. 2008;7:2749-61 pubmed
    ..These defects were additive with those conferred by the bub2 and mad2 spindle checkpoint mutations...
  50. Gibson D, Bell S, Aparicio O. Cell cycle execution point analysis of ORC function and characterization of the checkpoint response to ORC inactivation in Saccharomyces cerevisiae. Genes Cells. 2006;11:557-73 pubmed
    ..We discuss the potential significance of these overlapping checkpoints and the impact of our findings on previously postulated role(s) of ORCs in other cell cycle functions. ..
  51. Kim J, Song K. The study of Bfa1p(E438K) suggests that Bfa1 control the mitotic exit network in different mechanisms depending on different checkpoint-activating signals. Mol Cells. 2006;21:251-60 pubmed
    ..The MEN is regulated by a small GTPase, Tem1p, which in turn is controlled by a two-component GAP, Bfa1p-Bub2p. Recent results suggested that phosphorylation of Bfa1p by the polo-related kinase Cdc5p is also required for ..
  52. Toda K, Naito K, Mase S, Ueno M, Uritani M, Yamamoto A, et al. APC/C-Cdh1-dependent anaphase and telophase progression during mitotic slippage. Cell Div. 2012;7:4 pubmed publisher
    ..This is a problem for cancer chemotherapy using microtubule poisons. Here we describe mitotic slippage in yeast bub2? mutant cells that are defective in the repression of precocious telophase onset (mitotic exit)...
  53. Juanes M, Twyman H, Tunnacliffe E, Guo Z, ten Hoopen R, Segal M. Spindle pole body history intrinsically links pole identity with asymmetric fate in budding yeast. Curr Biol. 2013;23:1310-9 pubmed publisher
    ..Accordingly, the pattern of SPB inheritance was also randomized. Spc72 differential recruitment imparting asymmetric aMT organization represents the most upstream determinant linking SPB historical identity and fate...
  54. Jensen S, Johnson A, Johnston L, Segal M. Temporal coupling of spindle disassembly and cytokinesis is disrupted by deletion of LTE1 in budding yeast. Cell Cycle. 2004;3:817-22 pubmed
    ..Thus Lte1 function is important to fine-tune the timing of mitotic exit and to couple this event with cytokinesis in budding yeast. ..
  55. Höfken T, Schiebel E. Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2. J Cell Biol. 2004;164:219-31 pubmed
    ..exit defect of cdc5-10 Deltalte1 Deltagic1 Deltagic2 cells was rescued by inactivation of the inhibiting Bfa1-Bub2 GTPase-activating protein. Moreover, Gic1 bound directly to Bub2 and prevented binding of the GTPase Tem1 to Bub2...
  56. Jessulat M, Malty R, Nguyen Tran D, Deineko V, Aoki H, Vlasblom J, et al. Spindle Checkpoint Factors Bub1 and Bub2 Promote DNA Double-Strand Break Repair by Nonhomologous End Joining. Mol Cell Biol. 2015;35:2448-63 pubmed publisher
    ..genes associated with NHEJ deficiency upon disruption are two spindle assembly checkpoint kinases, Bub1 and Bub2. Both observation of resulting phenotypes and chromatin immunoprecipitation demonstrated that Bub1 and -2, either ..