Gene Symbol: BRX1
Description: Brx1p
Alias: Brx1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Wehner K, Baserga S. The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis. Mol Cell. 2002;9:329-39 pubmed
    ..The Imp4 superfamily is composed of five individual families (Imp4, Rpf1, Rpf2, Brx1, and Ssf) that all possess the sigma(70)-like motif, a eukaryotic RNA binding domain with prokaryotic origins...
  2. Miles T, Jakovljevic J, Horsey E, Harnpicharnchai P, Tang L, Woolford J. Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes. Mol Cell Biol. 2005;25:10419-32 pubmed
    ..Mutations in the WD40 motifs of Ytm1 disrupt binding to Erb1, destabilize the heterotrimer, and delay pre-rRNA processing and nuclear export of preribosomes. Nevertheless, 66S preribosomes lacking Ytm1 remain otherwise intact. ..
  3. Harnpicharnchai P, Jakovljevic J, Horsey E, Miles T, Roman J, Rout M, et al. Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol Cell. 2001;8:505-15 pubmed
    ..These results demonstrate that proteomic approaches in concert with genetic tools provide powerful means to purify and characterize ribosome assembly intermediates. ..
  4. Kaser A, Bogengruber E, Hallegger M, Doppler E, Lepperdinger G, Jantsch M, et al. Brix from xenopus laevis and brx1p from yeast define a new family of proteins involved in the biogenesis of large ribosomal subunits. Biol Chem. 2001;382:1637-47 pubmed
    ..In Saccharomyces cerevisiae, the essential gene YOL077c, now named BRX1, codes for the Brix homolog, which is also localized in the nucleolus...
  5. Bogengruber E, Briza P, Doppler E, Wimmer H, Koller L, Fasiolo F, et al. Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes. FEMS Yeast Res. 2003;3:35-43 pubmed
    An extensive homology search based on the sequence of the yeast protein Brx1p (biogenesis of ribosomes in Xenopus, YOL077c) revealed that it is a member of a superfamily of proteins sharing remarkable sequence similarities...
  6. Shimoji K, Jakovljevic J, Tsuchihashi K, Umeki Y, Wan K, Kawasaki S, et al. Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:4574-88 pubmed publisher
    ..Four different mutant alleles of the 60S ribosomal subunit assembly factor Brx1 were found...
  7. Wan K, Tsuchihashi K, Kanda K, Shimoji K, Mizuta K. N?-acetyltransferase NatA is involved in ribosome synthesis in Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 2013;77:631-8 pubmed
    ..mutants in which the second amino acid was substituted for proline in Ebp2 and functionally related proteins: Brx1, a partner of Ebp2 in ribosome biogenesis, and the ribosomal protein L36a/b, overexpression of which suppresses a ..
  8. Wan K, Yabuki Y, Mizuta K. Roles of Ebp2 and ribosomal protein L36 in ribosome biogenesis in Saccharomyces cerevisiae. Curr Genet. 2015;61:31-41 pubmed publisher
    ..8S rRNA. These results suggest that Ebp2 functions cooperatively with ribosomal proteins L36, L34, and L8 in biogenesis of the 60S ribosomal subunit. ..
  9. Fuentes J, Datta K, Sullivan S, Walker A, Maddock J. In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Mol Genet Genomics. 2007;278:105-23 pubmed
    ..Several conserved charge-to-alanine substitutions outside the GTPase domain display modest growth phenotypes indicating that these residues are not critical for function. ..

More Information


  1. Wan K, Kawara H, Yamamoto T, Kume K, Yabuki Y, Goshima T, et al. The essential function of Rrs1 in ribosome biogenesis is conserved in budding and fission yeasts. Yeast. 2015;32:607-14 pubmed publisher
    ..These results suggest that the essential function of Rrs1 in ribosome biogenesis may be conserved in budding and fission yeasts. ..
  2. Yang Y, Ting Y, Liang K, Lo K. The Roles of Puf6 and Loc1 in 60S Biogenesis Are Interdependent, and Both Are Required for Efficient Accommodation of Rpl43. J Biol Chem. 2016;291:19312-23 pubmed publisher
    ..Additionally, the recruitment of Rpl43 is required for the release of Puf6 and Loc1. We propose that Puf6 and Loc1 facilitate Rpl43 loading and are sequentially released from 60S after incorporation of Rpl43 into ribosomes in yeast. ..