Gene Symbol: BI4
Description: intron-encoded RNA maturase bI4
Alias: intron-encoded RNA maturase bI4
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Goguel V, Delahodde A, Jacq C. Connections between RNA splicing and DNA intron mobility in yeast mitochondria: RNA maturase and DNA endonuclease switching experiments. Mol Cell Biol. 1992;12:696-705 pubmed
    The intron-encoded proteins bI4 RNA maturase and aI4 DNA endonuclease can be faithfully expressed in yeast cytoplasm from engineered forms of their mitochondrial coding sequences...
  2. Herbert C, Labouesse M, Dujardin G, Slonimski P. The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases, and are involved in mRNA splicing. EMBO J. 1988;7:473-83 pubmed
  3. Li G, Becam A, Slonimski P, Herbert C. In vitro mutagenesis of the mitochondrial leucyl tRNA synthetase of Saccharomyces cerevisiae shows that the suppressor activity of the mutant proteins is related to the splicing function of the wild-type protein. Mol Gen Genet. 1996;252:667-75 pubmed
    ..tRNA synthetase (mLRS), which is necessary for the excision of the fourth intron of the mitochondrial cytb gene (bI4) and the fourth intron of the mitochondrial coxI gene (aI4), as well as for mitochondrial protein synthesis...
  4. Foury F, Roganti T, Lecrenier N, Purnelle B. The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae. FEBS Lett. 1998;440:325-31 pubmed
    ..Our data suggest that shuffling of mobile elements plays an important role in the remodelling of the yeast mitochondrial genome. ..
  5. Hsu J, Rho S, Vannella K, Martinis S. Functional divergence of a unique C-terminal domain of leucyl-tRNA synthetase to accommodate its splicing and aminoacylation roles. J Biol Chem. 2006;281:23075-82 pubmed
    ..acid C-terminal deletion of LeuRS, which does not complement a null strain, can form a ternary complex with the bI4 intron and its maturase splicing partner. However, the complex fails to stimulate splicing activity...
  6. Banroques J, Delahodde A, Jacq C. A mitochondrial RNA maturase gene transferred to the yeast nucleus can control mitochondrial mRNA splicing. Cell. 1986;46:837-44 pubmed
    b>bI4 maturase, encoded by the fourth intron of the yeast mitochondrial cytochrome b gene, controls the splicing of both the fourth intron of the cytochrome b gene and the fourth intron of the gene encoding subunit I of cytochrome oxidase...
  7. Nobrega F, Tzagoloff A. Assembly of the mitochondrial membrane system. DNA sequence and organization of the cytochrome b gene in Saccharomyces cerevisiae D273-10B. J Biol Chem. 1980;255:9828-37 pubmed
    ..The organization of the cytochrome b region in S. cerevisiae D273-10B is somewhat less complex than has been reported for other yeast strains i which exon b1 appears to be further fragmented into three smaller exons. ..
  8. Lambowitz A, Belfort M. Introns as mobile genetic elements. Annu Rev Biochem. 1993;62:587-622 pubmed
  9. Rho S, Martinis S. The bI4 group I intron binds directly to both its protein splicing partners, a tRNA synthetase and maturase, to facilitate RNA splicing activity. RNA. 2000;6:1882-94 pubmed
    ..NAM2p) and a mitochondrial-expressed intron-encoded maturase protein are required for splicing the fourth intron (bI4) of the yeast cob gene, which expresses an electron transfer protein that is essential to respiration...

More Information


  1. Rho S, Lincecum T, Martinis S. An inserted region of leucyl-tRNA synthetase plays a critical role in group I intron splicing. EMBO J. 2002;21:6874-81 pubmed
    Yeast mitochondrial leucyl-tRNA synthetase (LeuRS) binds to the bI4 intron and collaborates with the bI4 maturase to aid excision of the group I intron...
  2. Goguel V, Perea J, Jacq C. Synthesis and function of the mitochondrial intron--encoded bI4 RNA maturase from Saccharomyces cerevisiae. Effects of upstream frame-shift mutations. Curr Genet. 1989;16:241-6 pubmed
    We have analyzed the expression and function of the intron-encoded bI4 maturase when frame-shift mutations in the upstream exon alter the translational process...
  3. Labouesse M, Netter P, Schroeder R. Molecular basis of the 'box effect', A maturase deficiency leading to the absence of splicing of two introns located in two split genes of yeast mitochondrial DNA. Eur J Biochem. 1984;144:85-93 pubmed excise the fourth intron of oxi3 from the cytochrome oxidase subunit I pre-mRNA; the absence of a functional bI4 mRNA maturase, a trans-acting factor encoded by the fourth intron of the cob-box gene explains this phenomenon...
  4. Boniecki M, Rho S, Tukalo M, Hsu J, Romero E, Martinis S. Leucyl-tRNA synthetase-dependent and -independent activation of a group I intron. J Biol Chem. 2009;284:26243-50 pubmed publisher
    ..Intracellular experiments have suggested that it works in collaboration with a maturase that is encoded within the bI4 intron...