Gene Symbol: BEM3
Description: Bem3p
Alias: Bem3p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Zheng Y, Hart M, Shinjo K, Evans T, Bender A, Cerione R. Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain. J Biol Chem. 1993;268:24629-34 pubmed
    The Bem2 and Bem3 proteins, which appear to play roles in the regulation of bud site formation in Saccharomyces cerevisiae, show striking homology to a number of proteins that compose a family of GTPase-activating proteins (GAPs) for the ..
  2. Madden K, Snyder M. Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol. 1998;52:687-744 pubmed
    ..Since many of the components important for polarized cell growth are conserved in other organisms, the basic mechanisms mediating polarized cell growth are likely to be universal among eukaryotes. ..
  3. Johnson D. Cdc42: An essential Rho-type GTPase controlling eukaryotic cell polarity. Microbiol Mol Biol Rev. 1999;63:54-105 pubmed
    ..Future research should focus on this question as well as on the detailed analysis of the interactions of Cdc42p with its regulators and downstream effectors. ..
  4. Chenevert J, Valtz N, Herskowitz I. Identification of genes required for normal pheromone-induced cell polarization in Saccharomyces cerevisiae. Genetics. 1994;136:1287-96 pubmed
    ..A final group of mutants exhibits apparently normal shmoo morphology. The nature of their mating defect is yet to be determined. We discuss the possible roles of these gene products in establishing cell polarity during mating. ..
  5. Stevenson B, Ferguson B, De Virgilio C, Bi E, Pringle J, Ammerer G, et al. Mutation of RGA1, which encodes a putative GTPase-activating protein for the polarity-establishment protein Cdc42p, activates the pheromone-response pathway in the yeast Saccharomyces cerevisiae. Genes Dev. 1995;9:2949-63 pubmed
  6. Zheng Y, Cerione R, Bender A. Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3. J Biol Chem. 1994;269:2369-72 pubmed
    ..We also report the identification of a new gene, BEM3, that is a multicopy suppressor of the temperature-sensitive lethality caused by mutations in the bud emergence ..
  7. Knaus M, Pelli Gulli M, van Drogen F, Springer S, Jaquenoud M, Peter M. Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence. EMBO J. 2007;26:4501-13 pubmed
    ..Here we investigated the role and regulation of the GTPase-activating enzymes (GAPs) Bem2p and Bem3p for Cdc42p activation and actin polarization at bud emergence in Saccharomyces cerevisiae...
  8. Smith G, Givan S, Cullen P, Sprague G. GTPase-activating proteins for Cdc42. Eukaryot Cell. 2002;1:469-80 pubmed
    ..At the time this study began, there was one known GAP, Bem3, and one putative GAP, Rga1, for Cdc42...
  9. Bidlingmaier S, Snyder M. Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators. J Cell Biol. 2004;164:207-18 pubmed
    ..We found that the polarisome components Spa2, Pea2, and Bni1 and the Cdc42 regulators Cdc24 and Bem3 control the timing and frequency of projection formation...

More Information


  1. Caviston J, Longtine M, Pringle J, Bi E. The role of Cdc42p GTPase-activating proteins in assembly of the septin ring in yeast. Mol Biol Cell. 2003;14:4051-66 pubmed
    ..carrying the cdc42V36G allele or lacking two or all three of the known Cdc42p GTPase-activating proteins (GAPs: Bem3p, Rga1p, and Rga2p) could recruit the septins to the cell cortex but were blocked or delayed in forming a normal ..
  2. Bi E, Pringle J. ZDS1 and ZDS2, genes whose products may regulate Cdc42p in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:5264-75 pubmed
    ..As ZDS1 and ZDS2 have recently been identified also by numerous other groups studying a wide range of biological phenomena, the roles of Cdc42p in intracellular signaling may be more diverse than has previously been appreciated. ..
  3. Aguilar R, Longhi S, Shaw J, Yeh L, Kim S, SchOn A, et al. Epsin N-terminal homology domains perform an essential function regulating Cdc42 through binding Cdc42 GTPase-activating proteins. Proc Natl Acad Sci U S A. 2006;103:4116-21 pubmed
    ..Furthermore, the epsins contribute to regulation of specific Cdc42 signaling pathways in yeast cells. These data support a model in which the epsins function as spatial and temporal coordinators of endocytosis and cell polarity. ..
  4. Ho H, Lee H, Liao H, Chen M. Involvement of Saccharomyces cerevisiae Avo3p/Tsc11p in maintaining TOR complex 2 integrity and coupling to downstream signaling. Eukaryot Cell. 2008;7:1328-43 pubmed publisher
    ..Our results also suggest that Avo2p/Slm1p-mediated signaling, but not Avo1p-mediated signaling, links to Rho1p activation specifically through the Rho1p-guanine nucleotide exchange factor Tus1p. ..
  5. Mukherjee D, Coon B, Edwards D, Hanna C, Longhi S, McCaffery J, et al. The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway. J Cell Sci. 2009;122:2453-63 pubmed publisher
    ..induction of the phenotype and found them to be important for efficient binding to the septin regulatory protein, Bem3. Supporting a physiological role for epsin 2 in cell division, the protein localized to sites of polarized growth ..
  6. Yu J, Lemmon M. All phox homology (PX) domains from Saccharomyces cerevisiae specifically recognize phosphatidylinositol 3-phosphate. J Biol Chem. 2001;276:44179-84 pubmed
    ..Our results establish that PtdIns-3-P, and not other phosphoinositides, is the target of all PX domains in S. cerevisiae and suggest a role for PX domains in assembly of multiprotein complexes at specific membrane surfaces. ..
  7. Richman T, Johnson D. Saccharomyces cerevisiae cdc42p GTPase is involved in preventing the recurrence of bud emergence during the cell cycle. Mol Cell Biol. 2000;20:8548-59 pubmed
    ..Specifically, Cdc42(D38E)p showed reduced interactions with the Cla4p p21-activated protein kinase and the Bem3p GTPase-activating protein and cdc42(D38E) was the only mutant allele able to complement the Deltacdc42 null mutant...
  8. Neller J, Dünkler A, Rösler R, Johnsson N. A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip. J Cell Biol. 2015;208:71-87 pubmed publisher
    ..bud growth and show that Epo1p binds simultaneously to the Cdc42p guanosine triphosphatase-activating protein Bem3p. Deletion of EPO1 or deletion of BEM3 in a polarisome-deficient strain reduces the amount of cER at the tip...
  9. Chen G, Zheng L, Chan C. The LIM domain-containing Dbm1 GTPase-activating protein is required for normal cellular morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:1376-90 pubmed
    ..The nonaxial budding defect of dbm1 mutants can be rescued partially by overproduction of Bem3p and is exacerbated by its absence...
  10. Onishi M, Ko N, Nishihama R, Pringle J. Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis. J Cell Biol. 2013;202:311-29 pubmed publisher
    ..This work suggests a general role for the catalytically inactive transglutaminases of fungi and animals, some of which have previously been implicated in cytokinesis. ..
  11. Nie W, He F, Yuan S, Jia Z, Wang R, Gao X. Roles of an N-terminal coiled-coil-containing domain in the localization and function of Bem3, a Rho GTPase-activating protein in budding yeast. Fungal Genet Biol. 2017;99:40-51 pubmed publisher
    ..The budding yeast Bem3 is a GAP for Cdc42, a Rho GTPase crucial for actin and septin organization...
  12. Atkins B, Yoshida S, Saito K, Wu C, Lew D, Pellman D. Inhibition of Cdc42 during mitotic exit is required for cytokinesis. J Cell Biol. 2013;202:231-40 pubmed publisher
    ..The effects of Cdc42 hyperactivation are largely mediated by the Cdc42 effector p21-activated kinase Ste20. Inhibition of Cdc42 and related Rho guanosine triphosphatases may be a general feature of cytokinesis in eukaryotes. ..
  13. Moore T, Tanaka H, Kim H, Jeon N, Yi T. Yeast G-proteins mediate directional sensing and polarization behaviors in response to changes in pheromone gradient direction. Mol Biol Cell. 2013;24:521-34 pubmed publisher
    ..Thus we demonstrate that G-proteins modulate in a ligand-dependent manner two fundamental cell-polarity behaviors in response to gradient directional change. ..
  14. Saito K, Fujimura Kamada K, Hanamatsu H, Kato U, Umeda M, Kozminski K, et al. Transbilayer phospholipid flipping regulates Cdc42p signaling during polarized cell growth via Rga GTPase-activating proteins. Dev Cell. 2007;13:743-51 pubmed
    ..We propose that a redistribution of phospholipids to the inner leaflet of the plasma membrane triggers the dispersal of Cdc42p from the apical growth site, through activation of GAPs...
  15. Goryachev A, Pokhilko A. Computational model explains high activity and rapid cycling of Rho GTPases within protein complexes. PLoS Comput Biol. 2006;2:e172 pubmed
    ..Interestingly, we find that the cycling regimes are only weakly dependent on the concentration of GTPase itself. ..
  16. Laan L, Koschwanez J, Murray A. Evolutionary adaptation after crippling cell polarization follows reproducible trajectories. elife. 2015;4: pubmed publisher
  17. Kadota J, Yamamoto T, Yoshiuchi S, Bi E, Tanaka K. Septin ring assembly requires concerted action of polarisome components, a PAK kinase Cla4p, and the actin cytoskeleton in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:5329-45 pubmed
    ..Our results suggest that polarisome components and Cla4p are required for the initial assembly of the septin ring and that the actin cytoskeleton is involved in this process. ..