BDP1

Summary

Gene Symbol: BDP1
Description: transcription factor TFIIIB subunit BDP1
Alias: TFC5, TFC7, transcription factor TFIIIB subunit BDP1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kassavetis G, Nguyen S, Kobayashi R, Kumar A, Geiduschek E, Pisano M. Cloning, expression, and function of TFC5, the gene encoding the B" component of the Saccharomyces cerevisiae RNA polymerase III transcription factor TFIIIB. Proc Natl Acad Sci U S A. 1995;92:9786-90 pubmed
    b>TFC5, the unique and essential gene encoding the B" component of the Saccharomyces cerevisiae RNA polymerase III transcription factor (TF)IIIB has been cloned. It encodes a 594-amino acid protein (67,688 Da)...
  2. Kassavetis G, Joazeiro C, Pisano M, Geiduschek E, Colbert T, Hahn S, et al. The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIB. Cell. 1992;71:1055-64 pubmed
    ..Incorporation of the 90 kd B" protein into the transcription complex requires TBP. The heparin-resistant TFIIIB-DNA complex retains all three of its constituent proteins, TBP, BRF, and B". ..
  3. Hu H, Wu C, Lee J, Chen H. A Region of Bdp1 Necessary for Transcription Initiation That Is Located within the RNA Polymerase III Active Site Cleft. Mol Cell Biol. 2015;35:2831-40 pubmed publisher
    The RNA polymerase III (Pol III)-specific transcription factor Bdp1 is crucial to Pol III recruitment and promoter opening in transcription initiation, yet structural information is sparse...
  4. Khoo S, Wu C, Lin Y, Lee J, Chen H. Mapping the protein interaction network for TFIIB-related factor Brf1 in the RNA polymerase III preinitiation complex. Mol Cell Biol. 2014;34:551-9 pubmed publisher
    ..In contrast to the N-terminal domain of Brf1, the C-terminal domain contains extensive binding sites for TBP and Bdp1 to hold together the TFIIIB complex on the promoter...
  5. Milliman E, Hu Z, Yu M. Genomic insights of protein arginine methyltransferase Hmt1 binding reveals novel regulatory functions. BMC Genomics. 2012;13:728 pubmed publisher
    ..Co-immunoprecipitation studies demonstrate that Hmt1 interacts with the TFIIIB component Bdp1, suggesting a mechanism for Hmt1 in modulating RNA Pol III transcription to regulate tRNA production...
  6. Tavenet A, Suleau A, Dubreuil G, Ferrari R, Ducrot C, Michaut M, et al. Genome-wide location analysis reveals a role for Sub1 in RNA polymerase III transcription. Proc Natl Acad Sci U S A. 2009;106:14265-70 pubmed publisher
    ..Sub1 was required for optimal Pol III gene transcription in exponentially growing cells. ..
  7. Tsihlis N, Grove A. The Saccharomyces cerevisiae RNA polymerase III recruitment factor subunits Brf1 and Bdp1 impose a strict sequence preference for the downstream half of the TATA box. Nucleic Acids Res. 2006;34:5585-93 pubmed
    ..recruitment of Saccharomyces cerevisiae RNA polymerase III, TBP is associated with two additional factors, Brf1 and Bdp1, to form the initiation factor TFIIIB...
  8. Ishiguro A, Kassavetis G, Geiduschek E. Essential roles of Bdp1, a subunit of RNA polymerase III initiation factor TFIIIB, in transcription and tRNA processing. Mol Cell Biol. 2002;22:3264-75 pubmed
    The essential Saccharomyces cerevisiae gene BDP1 encodes a subunit of RNA polymerase III (Pol III) transcription factor (TFIIIB); TATA box binding protein (TBP) and Brf1 are the other subunits of this three-protein complex...
  9. Dumay Odelot H, Acker J, Arrebola R, Sentenac A, Marck C. Multiple roles of the tau131 subunit of yeast transcription factor IIIC (TFIIIC) in TFIIIB assembly. Mol Cell Biol. 2002;22:298-308 pubmed
    ..Copurification experiments of baculovirus-expressed proteins confirmed a direct physical interaction between tau131 and B". tau131, therefore, appears to be involved in the recruitment of both Brf1 and B". ..

More Information

Publications25

  1. Deprez E, Arrebola R, Conesa C, Sentenac A. A subunit of yeast TFIIIC participates in the recruitment of TATA-binding protein. Mol Cell Biol. 1999;19:8042-51 pubmed
    ..Coimmunoprecipitation experiments support a direct interaction between TBP and tau60. It is suggested that tau60 links tauA and tauB domains and participates in TFIIIB assembly via its interaction with TBP. ..
  2. Dumay H, Rubbi L, Sentenac A, Marck C. Interaction between yeast RNA polymerase III and transcription factor TFIIIC via ABC10alpha and tau131 subunits. J Biol Chem. 1999;274:33462-8 pubmed
    ..These results point to the potential importance of a functional contact between TFIIIC and RNA polymerase III. ..
  3. Andrau J, Sentenac A, Werner M. Mutagenesis of yeast TFIIIB70 reveals C-terminal residues critical for interaction with TBP and C34. J Mol Biol. 1999;288:511-20 pubmed
    ..TFIIIC.DNA complexes and affected in vitro transcription of the SUP4 tRNA gene. Our results underscore the important role of region II of TFIIIB70 in pre-initiation as well as transcription complex assembly via C34 and TBP binding. ..
  4. Yieh L, Kassavetis G, Geiduschek E, Sandmeyer S. The Brf and TATA-binding protein subunits of the RNA polymerase III transcription factor IIIB mediate position-specific integration of the gypsy-like element, Ty3. J Biol Chem. 2000;275:29800-7 pubmed
    ..These findings suggest that the minimal requirements for pol III transcription and Ty3 integration are very similar. ..
  5. Ishiguro A, Kassavetis G. A gene-specific effect of an internal deletion in the Bdp1 subunit of the RNA polymerase III transcription initiation factor TFIIIB. FEBS Lett. 2003;548:33-6 pubmed
    ..III, has been isolated as a multicopy suppressor of a specific small internal deletion (amino acids 253-269) in the Bdp1 subunit of transcription factor TFIIIB, the core pol III transcription factor...
  6. Liao Y, Willis I, Moir R. The Brf1 and Bdp1 subunits of transcription factor TFIIIB bind to overlapping sites in the tetratricopeptide repeats of Tfc4. J Biol Chem. 2003;278:44467-74 pubmed
    ..through interactions involving the Tfc4 subunit of the assembly factor TFIIIC and two subunits of TFIIIB, Brf1 and Bdp1. Tfc4 contains two arrays of tetratricopeptide repeats (TPRs), each of which provides a binding site for Brf1...
  7. Dieci G, Ruotolo R, Braglia P, Carles C, Carpentieri A, Amoresano A, et al. Positive modulation of RNA polymerase III transcription by ribosomal proteins. Biochem Biophys Res Commun. 2009;379:489-93 pubmed publisher
  8. Pratt Hyatt M, Pai D, Haeusler R, Wozniak G, Good P, Miller E, et al. Mod5 protein binds to tRNA gene complexes and affects local transcriptional silencing. Proc Natl Acad Sci U S A. 2013;110:E3081-9 pubmed publisher
    ..These results are discussed in light of recent results showing that silencing near tRNA genes also requires chromatin modification...
  9. Roberts S, Miller S, Lane W, Lee S, Hahn S. Cloning and functional characterization of the gene encoding the TFIIIB90 subunit of RNA polymerase III transcription factor TFIIIB. J Biol Chem. 1996;271:14903-9 pubmed
    ..Our results suggest that the binding sites for BRF1 and TFIIIB90 on TBP-DNA both overlap the binding sites for TFIIA and TFIIB. ..
  10. Lee J, Moir R, Willis I. Differential Phosphorylation of RNA Polymerase III and the Initiation Factor TFIIIB in Saccharomyces cerevisiae. PLoS ONE. 2015;10:e0127225 pubmed publisher
    ..of phosphorylation was observed for several of these proteins and the Rpc82 subunit of the polymerase and the Bdp1 subunit of TFIIIB were found to be differentially phosphorylated...
  11. Ruth J, Conesa C, Dieci G, Lefebvre O, Düsterhöft A, Ottonello S, et al. A suppressor of mutations in the class III transcription system encodes a component of yeast TFIIIB. EMBO J. 1996;15:1941-9 pubmed
    ..We report the isolation of the yeast gene TFC7 which, based on genetic and biochemical evidence, encodes the 90 kDa polypeptide...
  12. Kassavetis G, Letts G, Geiduschek E. The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter opening. EMBO J. 2001;20:2823-34 pubmed
    ..Removing an N-terminal domain of Brf interferes with downstream propagation of the transcription bubble to and beyond the transcriptional start site. ..
  13. Bachman N, Gelbart M, Tsukiyama T, Boeke J. TFIIIB subunit Bdp1p is required for periodic integration of the Ty1 retrotransposon and targeting of Isw2p to S. cerevisiae tDNAs. Genes Dev. 2005;19:955-64 pubmed
    ..This study provides the first example for recruitment of an ATP-dependent chromatin-remodeling factor by a general transcription factor in vivo. ..
  14. Kassavetis G, Driscoll R, Geiduschek E. Mapping the principal interaction site of the Brf1 and Bdp1 subunits of Saccharomyces cerevisiae TFIIIB. J Biol Chem. 2006;281:14321-9 pubmed
    ..Here, the principal attachment site of Brf1 for the Bdp1 subunit of TFIIIB has been mapped by a combination of structure-informed, site-directed mutagenesis and ..
  15. Saida F. Structural characterization of the interaction between TFIIIB components Bdp1 and Brf1. Biochemistry. 2008;47:13197-206 pubmed publisher
    ..Its three components (TBP, Brf1, and Bdp1) participate in crucial molecular events that include RNA polymerase recruitment, formation of the open initiation ..
  16. Wu C, Lin Y, Chen H. The TFIIF-like Rpc37/53 dimer lies at the center of a protein network to connect TFIIIC, Bdp1, and the RNA polymerase III active center. Mol Cell Biol. 2011;31:2715-28 pubmed publisher
    ..domain of Rpc37 interacts extensively with Rpc2 and Rpc34 and contains binding sites for initiation factor Bdp1. We also located the C-terminal domain of Rpc37 within the Pol III active center in the ternary elongation complex, ..