Gene Symbol: BDF1
Description: chromatin-binding protein BDF1
Alias: chromatin-binding protein BDF1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Pamblanco M, Poveda A, Sendra R, Rodriguez Navarro S, Pérez Ortín J, Tordera V. Bromodomain factor 1 (Bdf1) protein interacts with histones. FEBS Lett. 2001;496:31-5 pubmed
    ..Moreover, rBdf1p is able to bind histones H3 and H4 having different degrees of acetylation. Finally, we have not detected histone acetyltransferase activity associated with Bdf1p. ..
  2. Chua P, Roeder G. Bdf1, a yeast chromosomal protein required for sporulation. Mol Cell Biol. 1995;15:3685-96 pubmed
    The BDF1 gene of Saccharomyces cerevisiae is required for sporulation. Under starvation conditions, most cells from the bdf1 null mutant fail to undergo one or both meiotic divisions, and there is an absolute defect in spore formation...
  3. Chen L, Liu L, Wang M, Fu J, Zhang Z, Hou J, et al. Hal2p functions in Bdf1p-involved salt stress response in Saccharomyces cerevisiae. PLoS ONE. 2013;8:e62110 pubmed publisher
    ..Here, we reported that HAL2 overexpression recovered the salt stress sensitivity of bdf1?. Further evidence demonstrated that HAL2 expression was regulated indirectly by Bdf1p...
  4. Fu J, Hou J, Liu L, Chen L, Wang M, Shen Y, et al. Interplay between BDF1 and BDF2 and their roles in regulating the yeast salt stress response. FEBS J. 2013;280:1991-2001 pubmed publisher
    The homologous genes BDF1 and BDF2 in Saccharomyces cerevisiae encode bromodomain-containing transcription factors. Although double deletion of BDF1 and BDF2 is lethal, single deletion does not affect cell viability...
  5. Durant M, Pugh B. NuA4-directed chromatin transactions throughout the Saccharomyces cerevisiae genome. Mol Cell Biol. 2007;27:5327-35 pubmed
    ..Acetylated H3 and H4 tails have been implicated in binding bromodomain proteins, including Bdf1. Bdf1 interacts with the general transcription factor TFIID, which might promote preinitiation complex (PIC) ..
  6. Hassan A, Awad S, Al Natour Z, Othman S, Mustafa F, Rizvi T. Selective recognition of acetylated histones by bromodomains in transcriptional co-activators. Biochem J. 2007;402:125-33 pubmed
    ..Some bromodomains such as the Bdf1-2 do not interact with most of the acetylated peptides tested...
  7. Bianchi M, Costanzo G, Chelstowska A, Grabowska D, Mazzoni C, Piccinni E, et al. The bromodomain-containing protein Bdf1p acts as a phenotypic and transcriptional multicopy suppressor of YAF9 deletion in yeast. Mol Microbiol. 2004;53:953-68 pubmed
    ..suppressor genes, revealing that this phenotype can be suppressed by overdosing the transcription factors BDF1 and GAT1 in the yaf9Delta strain...
  8. Sawa C, Nedea E, Krogan N, Wada T, Handa H, Greenblatt J, et al. Bromodomain factor 1 (Bdf1) is phosphorylated by protein kinase CK2. Mol Cell Biol. 2004;24:4734-42 pubmed
    Bromodomain factor 1 (Bdf1) associates with Saccharomyces cerevisiae TFIID and corresponds to the C-terminal half of higher eukaryotic TAF1. It also associates with the SWR-C complex, which is important for Htz1 deposition...
  9. Martinez Campa C, Politis P, Moreau J, Kent N, Goodall J, Mellor J, et al. Precise nucleosome positioning and the TATA box dictate requirements for the histone H4 tail and the bromodomain factor Bdf1. Mol Cell. 2004;15:69-81 pubmed
    ..the acetylatable lysine residues of the histone H4 N-terminal region and on the TFIID-associated bromodomain factor Bdf1. This dependency arises either through fusion of the PHO5 promoter to a lacZ reporter or by mutation of the TATA ..

More Information


  1. Lygerou Z, Conesa C, Lesage P, Swanson R, Ruet A, Carlson M, et al. The yeast BDF1 gene encodes a transcription factor involved in the expression of a broad class of genes including snRNAs. Nucleic Acids Res. 1994;22:5332-40 pubmed
    ..This mutant defines a new gene, named BDF1. In a bdf1-1 strain, the reporter snRNA synthesized before the temperature shift remains stable at the non-..
  2. Liu X, Yang H, Zhang X, Liu L, Lei M, Zhang Z, et al. Bdf1p deletion affects mitochondrial function and causes apoptotic cell death under salt stress. FEMS Yeast Res. 2009;9:240-6 pubmed publisher
    The Saccharomyces cerevisiae BDF1 gene encodes a bromodomain-containing transcription factor. We previously reported that deletion of Bdf1p in yeast cells resulted in increased sensitivity to NaCl stress...
  3. Liu X, Zhang X, Wang C, Liu L, Lei M, Bao X. Genetic and comparative transcriptome analysis of bromodomain factor 1 in the salt stress response of Saccharomyces cerevisiae. Curr Microbiol. 2007;54:325-30 pubmed
    The Saccharomyces cerevisiae BDF1 gene, which encodes a bromodomain-containing transcription factor, was previously isolated by transposon mutugenesis in a screen for salt-sensitive mutants...
  4. Kobor M, Venkatasubrahmanyam S, Meneghini M, Gin J, Jennings J, Link A, et al. A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin. PLoS Biol. 2004;2:E131 pubmed
    ..Metazoan orthologs of Swr1p (Drosophila Domino; human SRCAP and p400) may have analogous functions...
  5. Matangkasombut O, Buratowski R, Swilling N, Buratowski S. Bromodomain factor 1 corresponds to a missing piece of yeast TFIID. Genes Dev. 2000;14:951-62 pubmed
    ..Using a yeast two-hybrid screen to identify proteins that interact with Taf67, we isolated Bromodomain factor 1 (Bdf1) and its homolog (Bdf2)...
  6. Ladurner A, Inouye C, Jain R, Tjian R. Bromodomains mediate an acetyl-histone encoded antisilencing function at heterochromatin boundaries. Mol Cell. 2003;11:365-76 pubmed
    ..Here we show that yeast Bdf1 bromodomains recognize endogenous acetyl-histone H3/H4 as a mechanism for chromatin association in vivo...
  7. Matangkasombut O, Buratowski S. Different sensitivities of bromodomain factors 1 and 2 to histone H4 acetylation. Mol Cell. 2003;11:353-63 pubmed
    ..Bromodomain Factor 1 (Bdf1), a protein that associates with TFIID, binds histone H4 with preference for multiply acetylated forms...
  8. Zhang H, Roberts D, Cairns B. Genome-wide dynamics of Htz1, a histone H2A variant that poises repressed/basal promoters for activation through histone loss. Cell. 2005;123:219-31 pubmed
    ..histone modifications, and Htz1 deposition is partially reliant on Gcn5 (a histone acetyltransferase) and Bdf1, an SWR1 complex member that binds acetylated histones...
  9. García Oliver E, Ramus C, Perot J, Arlotto M, Champleboux M, Mietton F, et al. Bdf1 Bromodomains Are Essential for Meiosis and the Expression of Meiotic-Specific Genes. PLoS Genet. 2017;13:e1006541 pubmed publisher
    ..They are present in unicellular eukaryotes and in this study, the role of the BET protein Bdf1 has been explored in Saccharomyces cerevisiae...
  10. Chen L, Wang M, Hou J, Fu J, Shen Y, Liu F, et al. HAL2 overexpression induces iron acquisition in bdf1? cells and enhances their salt resistance. Curr Genet. 2017;63:229-239 pubmed publisher
    ..the molecular mechanism of salt stress response in yeast, especially the molecular mechanism related to genes BDF1 and HAL2...
  11. Chen L, Wang M, Hou J, Liu L, Fu J, Shen Y, et al. Regulation of Saccharomyces cerevisiae MEF1 by Hda1p affects salt resistance of bdf1Δ mutant. FEMS Yeast Res. 2014;14:575-85 pubmed
    ..In this study, we reported that the salt sensitivity, mitochondrial dysfunction, and nuclear instability of bdf1Δ mutant were suppressed by HDA1 deletion or MEF1 overexpression...
  12. Koerber R, Rhee H, Jiang C, Pugh B. Interaction of transcriptional regulators with specific nucleosomes across the Saccharomyces genome. Mol Cell. 2009;35:889-902 pubmed publisher
    ..we examine on a genomic scale several protein-nucleosome interactions, including those that (1) bind histones (Bdf1/SWR1 and Srm1), (2) bind specific DNA sequences (Rap1 and Reb1), and (3) potentially collide with nucleosomes ..
  13. Liu Y, Kung C, Fishburn J, Ansari A, Shokat K, Hahn S. Two cyclin-dependent kinases promote RNA polymerase II transcription and formation of the scaffold complex. Mol Cell Biol. 2004;24:1721-35 pubmed
    ..In addition to the previously known substrate, the Pol II CTD, it was found that Kin28 phosphorylates two subunits of Mediator and Srb10 targets two subunits of TFIID for phosphorylation. ..
  14. Poveda A, Pamblanco M, Tafrov S, Tordera V, Sternglanz R, Sendra R. Hif1 is a component of yeast histone acetyltransferase B, a complex mainly localized in the nucleus. J Biol Chem. 2004;279:16033-43 pubmed
  15. Wu W, Wu C, Ladurner A, Mizuguchi G, Wei D, Xiao H, et al. N terminus of Swr1 binds to histone H2AZ and provides a platform for subunit assembly in the chromatin remodeling complex. J Biol Chem. 2009;284:6200-7 pubmed publisher
    ..Here we examined the function of three additional SWR1 subunits, bromodomain containing Bdf1, actin-related protein Arp4 and Swc7, by analyzing affinity-purified mutant SWR1 complexes...
  16. Chimura T, Kuzuhara T, Horikoshi M. Identification and characterization of CIA/ASF1 as an interactor of bromodomains associated with TFIID. Proc Natl Acad Sci U S A. 2002;99:9334-9 pubmed
    ..Furthermore, yeast strain lacking the BDF1 gene shows the Spt phenotype that is shown also by the ASF1 gene disruptant, and a double-knockout strain of both ..
  17. Ranjan A, Mizuguchi G, FitzGerald P, Wei D, Wang F, Huang Y, et al. Nucleosome-free region dominates histone acetylation in targeting SWR1 to promoters for H2A.Z replacement. Cell. 2013;154:1232-45 pubmed publisher
  18. Luk E, Vu N, Patteson K, Mizuguchi G, Wu W, Ranjan A, et al. Chz1, a nuclear chaperone for histone H2AZ. Mol Cell. 2007;25:357-68 pubmed
    ..The presence of this motif in other metazoan proteins suggests that H2AZ-specific chaperones may be widely conserved. ..
  19. Nadal Ribelles M, Mas G, Millán Zambrano G, Solé C, Ammerer G, Chávez S, et al. H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes. Nucleic Acids Res. 2015;43:4937-49 pubmed publisher
    ..Our findings point to a novel role for H3K4 monomethylation in dictating the specificity of chromatin remodeling, adding an extra layer of regulation to the transcriptional stress response. ..
  20. Watanabe S, Tan D, Lakshminarasimhan M, Washburn M, Hong E, Walz T, et al. Structural analyses of the chromatin remodelling enzymes INO80-C and SWR-C. Nat Commun. 2015;6:7108 pubmed publisher
    ..In contrast, the Ies6/Arp5 module is essential for INO80-C remodelling, and furthermore this module controls conformational changes that may couple nucleosome binding to remodelling. ..
  21. Raisner R, Hartley P, Meneghini M, Bao M, Liu C, Schreiber S, et al. Histone variant H2A.Z marks the 5' ends of both active and inactive genes in euchromatin. Cell. 2005;123:233-48 pubmed
    ..Efficient deposition of H2A.Z is further promoted by a specific pattern of histone H3 and H4 tail acetylation and the bromodomain protein Bdf1, a component of the Swr1 remodeling complex that deposits H2A.Z.
  22. Zhang Q, Chakravarty S, Ghersi D, Zeng L, Plotnikov A, Sanchez R, et al. Biochemical profiling of histone binding selectivity of the yeast bromodomain family. PLoS ONE. 2010;5:e8903 pubmed publisher
    ..Finally, electrostatic interactions appear to be a primary determining factor that guides productive association between a bromodomain and a lysine-acetylated histone. ..
  23. Akai Y, Adachi N, Hayashi Y, Eitoku M, Sano N, Natsume R, et al. Structure of the histone chaperone CIA/ASF1-double bromodomain complex linking histone modifications and site-specific histone eviction. Proc Natl Acad Sci U S A. 2010;107:8153-8 pubmed publisher
    ..The model we propose here is the initial structure-based model of the biological signaling from histone modifications to structural change of the nucleosome (hi-MOST model). ..
  24. Pattenden S, Gogol M, Workman J. Features of cryptic promoters and their varied reliance on bromodomain-containing factors. PLoS ONE. 2010;5:e12927 pubmed publisher
    ..Specifically, we looked at the dependence of promoters on the bromodomain-containing RSC complex and the Bdf1 protein. We found that the dependence of cryptic promoters for these proteins varied...
  25. Roy K, Chanfreau G. Stress-induced nuclear RNA degradation pathways regulate yeast bromodomain factor 2 to promote cell survival. PLoS Genet. 2014;10:e1004661 pubmed publisher
  26. Fu J, Hou J, Chen L, Wang M, Shen Y, Zhang Z, et al. The yeast BDF1 regulates endocytosis via LSP1 under salt stress. Curr Microbiol. 2015;70:671-8 pubmed publisher
    ..The homologous genes, BDF1 and BDF2, in Saccharomyces cerevisiae, respectively, encode a bromodomain-containing transcription factor...