Genomes and Genes
Gene Symbol: BCY1
Description: cAMP-dependent protein kinase regulatory subunit BCY1
Alias: SRA1, cAMP-dependent protein kinase regulatory subunit BCY1
Species: Saccharomyces cerevisiae S288c
- Toda T, Cameron S, Sass P, Zoller M, Scott J, McMullen B, et al. Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. Mol Cell Biol. 1987;7:1371-7 pubmedWe have cloned a gene (BCY1) from the yeast Saccharomyces cerevisiae that encodes a regulatory subunit of the cyclic AMP-dependent protein kinase...
- Umekawa M, Ujihara M, Makishima K, Yamamoto S, Takematsu H, Wakayama M. The signaling pathways underlying starvation-induced upregulation of Î±-mannosidase Ams1 in Saccharomyces cerevisiae. Biochim Biophys Acta. 2016;1860:1192-201 pubmed publisher..The signaling molecules responsible for regulation of Ams1 were also clarified. ..
- GonzÃ¡lez Bardeci N, Caramelo J, Blumenthal D, Rinaldi J, Rossi S, Moreno S. The PKA regulatory subunit from yeast forms a homotetramer: Low-resolution structure of the N-terminal oligomerization domain. J Struct Biol. 2016;193:141-54 pubmed publisher..In this work, we present the structural analysis of the D/D domain of Bcy1, the R subunit of PKA from Saccharomyces cerevisiae...
- Stefan E, Malleshaiah M, Breton B, Ear P, Bachmann V, Beyermann M, et al. PKA regulatory subunits mediate synergy among conserved G-protein-coupled receptor cascades. Nat Commun. 2011;2:598 pubmed publisher..These findings suggest a direct mechanism by which coincident activation of G?s-coupled receptors controls the precision of adaptive responses of activated G?i-coupled receptor cascades. ..
- Searle J, Wood M, Kaur M, Tobin D, Sanchez Y. Proteins in the nutrient-sensing and DNA damage checkpoint pathways cooperate to restrain mitotic progression following DNA damage. PLoS Genet. 2011;7:e1002176 pubmed publisher..Our data indicate that there is cross-talk between the DNA damage checkpoint and the proteins that integrate nutrient and stress signals to regulate PKA. ..
- Tripp M, Pinon R. Control of the cAMP pathway by the cell cycle start function, CDC25, in Saccharomyces cerevisiae. J Gen Microbiol. 1986;132:1143-51 pubmed..The mutant bcy1 [a lesion resulting in a low level of regulatory (R) subunit and a high level of active, catalytic (C) subunit of ..
- Matsuura A, Treinin M, Mitsuzawa H, Kassir Y, Uno I, Simchen G. The adenylate cyclase/protein kinase cascade regulates entry into meiosis in Saccharomyces cerevisiae through the gene IME1. EMBO J. 1990;9:3225-32 pubmed..Multicopy IME1 plasmids overcome the meiotic deficiency of bcy1 and of RASval19 diploids. Double mutants ime1 cdc25 and ime1 ras2 are sporulation deficient...
- Zhang A, Gao W. Mechanisms of protein kinase Sch9 regulating Bcy1 in Saccharomyces cerevisiae. FEMS Microbiol Lett. 2012;331:10-6 pubmed publisherIn this study, we investigated the mechanisms of Sch9 regulating the localization and phosphorylation of Bcy1. Our research indicated that Sch9 regulated localization of Bcy1 via Zds1 for the following reasons: (1) deletions of SCH9 or ..
- Yoshida S, Ichihashi R, Toh e A. Ras recruits mitotic exit regulator Lte1 to the bud cortex in budding yeast. J Cell Biol. 2003;161:889-97 pubmed..These results suggest that Lte1 is a downstream effector protein of Ras in mitotic exit and that the Ras GEF domain of Lte1 is not essential for mitotic exit but required for its localization. ..
- Reinders A, Bürckert N, Boller T, Wiemken A, De Virgilio C. Saccharomyces cerevisiae cAMP-dependent protein kinase controls entry into stationary phase through the Rim15p protein kinase. Genes Dev. 1998;12:2943-55 pubmed..Taken together, these results place Rim15p immediately downstream and under negative control of cAPK and define a positive regulatory role of Rim15p for entry into both meiosis and stationary phase. ..
- Nehlin J, Carlberg M, Ronne H. Yeast SKO1 gene encodes a bZIP protein that binds to the CRE motif and acts as a repressor of transcription. Nucleic Acids Res. 1992;20:5271-8 pubmed..Third, in the absence of MIG1, there is also a rapid but transient glucose induction of the SUC2 mRNA. This induction is correlated with a transient loss of SKO1-dependent repression. ..
- Uno I, Oshima T, Ishikawa T. Localization of the regulatory subunit of cAMP-dependent protein kinase in Saccharomyces cerevisiae. Exp Cell Res. 1988;176:360-5 pubmed..cerevisiae cells was determined by subcellular fractionation and indirect immunofluorescence microscopy using the bcy1 mutant deficient in the regulatory subunit as control...
- Cai Y, Wei Y. Distinct regulation of Maf1 for lifespan extension by Protein kinase A and Sch9. Aging (Albany NY). 2015;7:133-43 pubmed..However, here we find that activating PKA by bcy1 deletion cannot replace Sch9 for Maf1 phosphorylation and cytoplasmic retention; instead, such modulation lowers ..
- Budhwar R, Lu A, Hirsch J. Nutrient control of yeast PKA activity involves opposing effects on phosphorylation of the Bcy1 regulatory subunit. Mol Biol Cell. 2010;21:3749-58 pubmed publisher..Here we show that Gpb1 and Gpb2 stimulate phosphorylation of PKA regulatory subunit Bcy1 in low glucose concentrations, thereby promoting the inhibitory function of Bcy1 when nutrients are scarce and PKA ..
- Mazon M, Behrens M, Morgado E, Portillo F. Low activity of the yeast cAMP-dependent protein kinase catalytic subunit Tpk3 is due to the poor expression of the TPK3 gene. Eur J Biochem. 1993;213:501-6 pubmed..of the inducible GAL1 promoter did not by itself result in a corresponding increase in activity but coexpression of BCY1, the gene coding for the regulatory subunit, was necessary in both cases to achieve high levels of phosphorylating ..
- Rinaldi J, Ocampo J, Rossi S, Moreno S. A novel activating effect of the regulatory subunit of protein kinase A on catalytic subunit activity. Arch Biochem Biophys. 2008;480:95-103 pubmed publisher..The behavior was mimicked by intact R. We conclude that in addition to its negative regulatory role, the R subunit stimulates C activity via distal interactions. ..
- Shirayama M, Kawakami K, Matsui Y, Tanaka K, Toh e A. MSI3, a multicopy suppressor of mutants hyperactivated in the RAS-cAMP pathway, encodes a novel HSP70 protein of Saccharomyces cerevisiae. Mol Gen Genet. 1993;240:323-32 pubmed..Overexpression of MSI3 also suppresses the heat shock-sensitive phenotype of the bcy1 mutant. Determination of the DNA sequence of MSI3 revealed that MSI3 can encode a 77...
- Broek D, Samiy N, Fasano O, Fujiyama A, Tamanoi F, Northup J, et al. Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins. Cell. 1985;41:763-9 pubmed..Furthermore, we observe a biochemical difference between the RAS2 and RAS2val19 proteins in their ability to activate adenylate cyclase after preincubation with GTP. ..
- Ma L, Ho K, Piggott N, Luo Z, Measday V. Interactions between the kinetochore complex and the protein kinase A pathway in Saccharomyces cerevisiae. G3 (Bethesda). 2012;2:831-41 pubmed publisher..Overall, our data suggest that the increased protein kinase A activity in kinetochore mutants is detrimental to cellular growth and chromosome transmission fidelity. ..
- Fujimura K, Tanaka K, Nakano A, Toh e A. The Saccharomyces cerevisiae MSI4 gene encodes the yeast counterpart of component A of Rab geranylgeranyltransferase. J Biol Chem. 1994;269:9205-12 pubmed..We propose that the MSI4 gene product is a yeast counterpart of component A of Rab geranylgeranyltransferase that is essential for Ypt1p to localize on membranes...
- Toda T, Uno I, Ishikawa T, Powers S, Kataoka T, Broek D, et al. In yeast, RAS proteins are controlling elements of adenylate cyclase. Cell. 1985;40:27-36 pubmed..of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1. Yeast strains carrying the IAC mutation have elevated levels of adenylate cyclase activity...
- Matsumoto K, Uno I, Ishikawa T. Control of cell division in Saccharomyces cerevisiae mutants defective in adenylate cyclase and cAMP-dependent protein kinase. Exp Cell Res. 1983;146:151-61 pubmed..The G1 arrest caused by the cyr1 mutation was overcome by the presence of a suppressor mutation, bcy1, that resulted in deficiency of a regulatory subunit of cAMP-dependent protein kinase and production of high level ..
- Kafadar K, Cyert M. Integration of stress responses: modulation of calcineurin signaling in Saccharomyces cerevisiae by protein kinase A. Eukaryot Cell. 2004;3:1147-53 pubmed..Thus, these studies identify a specific biochemical mechanism through which the activities of multiple stress-activated signaling pathways are integrated in vivo. ..
- Makanae K, Kintaka R, Makino T, Kitano H, Moriya H. Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method. Genome Res. 2013;23:300-11 pubmed publisher..The results obtained in this study will provide basic knowledge about the physiology of chromosomal abnormalities and the evolution of chromosomal composition. ..
- Sundaram V, Petkova M, Pujol Carrion N, Boada J, De La Torre Ruiz M. Tor1, Sch9 and PKA downregulation in quiescence rely on Mtl1 to preserve mitochondrial integrity and cell survival. Mol Microbiol. 2015;97:93-109 pubmed publisher..Mtl1 also plays a role in the regulation of both Bcy1 stability and phosphorylation, mainly in response to glucose depletion...
- Matsumoto K, Uno I, Oshima Y, Ishikawa T. Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1982;79:2355-9 pubmed..The cyr1 mutation was suppressed by a secondary mutation designated bcy1. The bcy1 mutation bypassed the need for cAMP for growth...
- Sasaki T, Toh e A, Kikuchi Y. Extragenic suppressors that rescue defects in the heat stress response of the budding yeast mutant tom1. Mol Gen Genet. 2000;262:940-8 pubmed..was found that the temperature sensitivity of the tom1-2 mutant is indeed suppressed by multiple copies of PDE2 or BCY1, which encode negative regulators of the cAMP/PKA pathway...
- Bharucha N, Ma J, Dobry C, Lawson S, Yang Z, Kumar A. Analysis of the yeast kinome reveals a network of regulated protein localization during filamentous growth. Mol Biol Cell. 2008;19:2708-17 pubmed publisher..Thus, the localization of Ksp1p and the other kinases identified here is tightly controlled during filamentous growth, representing an overlooked regulatory component of this stress response. ..
- Brenner C, Nakayama N, Goebl M, Tanaka K, Toh e A, Matsumoto K. CDC33 encodes mRNA cap-binding protein eIF-4E of Saccharomyces cerevisiae. Mol Cell Biol. 1988;8:3556-9 pubmedThe bcy1 mutation makes the cdc33 start mutant arrest at random points in the cell cycle instead of only at G1. We cloned and sequenced CDC33...
- Low Y, Bircham P, Maass D, Atkinson P. Kinetochore genes are required to fully activate secretory pathway expansion in S. cerevisiae under induced ER stress. Mol Biosyst. 2014;10:1790-802 pubmed publisher..This study concludes that functional kinetochores are required for full activation of the secretory pathway in elevated ER stress and that the responses to basal and elevated levels of ER stress require different networks of genes. ..
- Howard S, Chang Y, Budovskaya Y, Herman P. The Ras/PKA signaling pathway of Saccharomyces cerevisiae exhibits a functional interaction with the Sin4p complex of the RNA polymerase II holoenzyme. Genetics. 2001;159:77-89 pubmed..In all, our data suggest that specific proteins within the RNA polymerase II holoenzyme might be targets of signal transduction pathways that are responsible for coordinating gene expression with cell growth. ..
- Timblin B, Tatchell K, Bergman L. Deletion of the gene encoding the cyclin-dependent protein kinase Pho85 alters glycogen metabolism in Saccharomyces cerevisiae. Genetics. 1996;143:57-66 pubmed
- Haesendonckx S, Tudisca V, Voordeckers K, Moreno S, Thevelein J, Portela P. The activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinases in Saccharomyces cerevisiae. Biochem J. 2012;448:307-20 pubmed publisher..Pkh inactivation reduces the interaction of each catalytic subunit with the regulatory subunit Bcy1 without affecting the specific kinase activity of PKA...
- Wilson R, Brenner A, White T, Engler M, Gaughran J, Tatchell K. The Saccharomyces cerevisiae SRK1 gene, a suppressor of bcy1 and ins1, may be involved in protein phosphatase function. Mol Cell Biol. 1991;11:3369-73 pubmed..The ability of SRK1 to alleviate partially the defects caused by high levels of cyclic AMP-dependent protein kinase and the similarity of its encoded protein to dis3+ suggest that SRK1 may have a role in protein phosphatase function. ..
- Sobering A, Jung U, Lee K, Levin D. Yeast Rpi1 is a putative transcriptional regulator that contributes to preparation for stationary phase. Eukaryot Cell. 2002;1:56-65 pubmed..Finally, we propose that inappropriate expression of RPI1 during log phase growth drives fortification of the cell wall and that this behavior is responsible for suppression of the mpkl cell lysis defect. ..
- Mandal A, Lee P, Chen J, Nillegoda N, Heller A, DiStasio S, et al. Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation. J Cell Biol. 2007;176:319-28 pubmed..Our studies demonstrate that Cdc37 has a general role in kinome biogenesis. ..
- Tatchell K, Robinson L, Breitenbach M. RAS2 of Saccharomyces cerevisiae is required for gluconeogenic growth and proper response to nutrient limitation. Proc Natl Acad Sci U S A. 1985;82:3785-9 pubmed..The phenotype of the RAS2 mutant and extragenic suppressors implicate RAS with some function in the normal response to nutrient limitation. ..
- Behrens M, Mazon M. Yeast cAMP-dependent protein kinase can be associated to the plasma membrane. Biochem Biophys Res Commun. 1988;151:561-7 pubmed..Incubation of the plasma membrane with [gamma-32P]ATP results in the phosphorylation of the regulatory subunit. ..
- Sutton A, Immanuel D, Arndt K. The SIT4 protein phosphatase functions in late G1 for progression into S phase. Mol Cell Biol. 1991;11:2133-48 pubmed..due to a deletion of SIT4 and can also partially suppress the phenotypic defects due to a null mutation in BCY1. The SSD1 protein is implicated in G1 control and has a region of similarity to the dis3 protein of ..
- Baccarini L, MartÃnez MontaÃ±Ã©s F, Rossi S, Proft M, Portela P. PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae. Biochim Biophys Acta. 2015;1849:1329-39 pubmed publisher..are three PKA catalytic subunits in Saccharomyces cerevisiae: Tpk1, Tpk2, and Tpk3 and one regulatory subunit: Bcy1. Previously, it has been demonstrated that Tpk1 and Tpk2 are associated with coding regions and promoters of target ..
- Lesuisse E, Horion B, Labbe P, Hilger F. The plasma membrane ferrireductase activity of Saccharomyces cerevisiae is partially controlled by cyclic AMP. Biochem J. 1991;280 ( Pt 2):545-8 pubmed..This phenotype was suppressed by the bcy1 mutation in ras2 but not in ras1 mutants...
- Ramachandran V, Shah K, Herman P. The cAMP-dependent protein kinase signaling pathway is a key regulator of P body foci formation. Mol Cell. 2011;43:973-81 pubmed publisher..This work therefore highlights the general relevance of RNP foci in quiescent cells, and provides a framework for the study of the many RNP assemblies that form in eukaryotic cells. ..
- Rao S, Schmidt O, Harbauer A, Sch nfisch B, Guiard B, Pfanner N, et al. Biogenesis of the preprotein translocase of the outer mitochondrial membrane: protein kinase A phosphorylates the precursor of Tom40 and impairs its import. Mol Biol Cell. 2012;23:1618-27 pubmed publisher..We conclude that PKA plays a dual role in the regulation of the TOM complex. Phosphorylation by PKA not only impairs the receptor activity of Tom70, but it also inhibits the biogenesis of the channel protein Tom40...
- Córcoles Sáez I, Ballester Tomás L, de la Torre Ruiz M, Prieto J, Randez Gil F. Low temperature highlights the functional role of the cell wall integrity pathway in the regulation of growth in Saccharomyces cerevisiae. Biochem J. 2012;446:477-88 pubmed publisher..the phenotype of slt2? and bck1? mutant cells, as well as attenuation of PKA activity by overexpression of BCY1 (bypass of cyclase mutations 1)...
- Uno I, Matsumoto K, Ishikawa T. Characterization of cyclic AMP-requiring yeast mutants altered in the regulatory subunit of protein kinase. J Biol Chem. 1982;257:14110-5 pubmed..The CYR3 mutation was partially dominant over the wild type counterpart and suppressed by the bcy1 mutation which is responsible for the deficiency of the regulatory subunit of cAMP-dependent protein kinase...