Gene Symbol: ATP8
Description: F1F0 ATP synthase subunit 8
Alias: AAP1, F1F0 ATP synthase subunit 8
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Simon M, Faye G. Organization and processing of the mitochondrial oxi3/oli2 multigenic transcript in yeast. Mol Gen Genet. 1984;196:266-74 pubmed
    ..Furthermore, we have shown that a primary polycistronic transcript covers oxi3, aap1, oli2 and extends beyond URF2. Transcriptional analysis of this region revealed several cleavage points...
  2. Gulati S, Balderes D, Kim C, Guo Z, Wilcox L, Area Gomez E, et al. ATP-binding cassette transporters and sterol O-acyltransferases interact at membrane microdomains to modulate sterol uptake and esterification. FASEB J. 2015;29:4682-94 pubmed publisher
  3. Perez Martinez X, Butler C, Shingu Vazquez M, Fox T. Dual functions of Mss51 couple synthesis of Cox1 to assembly of cytochrome c oxidase in Saccharomyces cerevisiae mitochondria. Mol Biol Cell. 2009;20:4371-80 pubmed publisher
    ..Regulation of COX1 mRNA translation by Mss51 seems to be an example of a homeostatic mechanism in which a positive effector of gene expression interacts with the product it regulates in a posttranslational assembly process. ..
  4. Lau W, Baker L, Rubinstein J. Cryo-EM structure of the yeast ATP synthase. J Mol Biol. 2008;382:1256-64 pubmed publisher
    ..This new map has allowed construction of a model of subunit arrangement in the F(O) motor of ATP synthase that dictates how dimerization of the complex via subunits e and g might occur. ..
  5. Michon T, Galante M, Velours J. NH2-terminal sequence of the isolated yeast ATP synthase subunit 6 reveals post-translational cleavage. Eur J Biochem. 1988;172:621-5 pubmed
    ..Thus, mature subunit 6 contains 249 amino acid residues and displays a molecular mass of 27943 Da. ..
  6. Bueler S, Rubinstein J. Location of subunit d in the peripheral stalk of the ATP synthase from Saccharomyces cerevisiae. Biochemistry. 2008;47:11804-10 pubmed publisher
    ..We propose that the C-terminal region of subunit d spans the gap from F 1 to F O, reinforcing this section of the peripheral stalk. ..
  7. Barros M, Tzagoloff A. Aep3p-dependent translation of yeast mitochondrial ATP8. Mol Biol Cell. 2017;28:1426-1434 pubmed publisher
    ..Here we find that Aep3p, previously shown to stabilize the bicistronic ATP8-ATP6 mRNA and facilitate initiation of translation from unformylated methionine, also activates specifically ..
  8. Zamudio Ochoa A, Camacho Villasana Y, García Guerrero A, Pérez Martínez X. The Pet309 pentatricopeptide repeat motifs mediate efficient binding to the mitochondrial COX1 transcript in yeast. RNA Biol. 2014;11:953-67 pubmed publisher
    ..with the COX1 mRNA in vivo and that this association is present before processing of the COX1 mRNA from the ATP8/6 polycistronic mRNA...
  9. Rak M, Gokova S, Tzagoloff A. Modular assembly of yeast mitochondrial ATP synthase. EMBO J. 2011;30:920-30 pubmed publisher
    ..These studies show that assembly of the ATP synthase is not a single linear process, as previously thought, but rather involves two separate but coordinately regulated pathways that converge at the end stage. ..

More Information


  1. Horn D, Fontanesi F, Barrientos A. Exploring protein-protein interactions involving newly synthesized mitochondrial DNA-encoded proteins. Methods Mol Biol. 2008;457:125-39 pubmed
  2. Tzagoloff A, Barrientos A, Neupert W, Herrmann J. Atp10p assists assembly of Atp6p into the F0 unit of the yeast mitochondrial ATPase. J Biol Chem. 2004;279:19775-80 pubmed
    ..Based on these observations, we propose Atp10p to be an Atp6p-specific chaperone that facilitates the incorporation of Atp6p into an intermediate subcomplex of ATPase subunits. ..
  3. Arnold I, Pfeiffer K, Neupert W, Stuart R, Schagger H. ATP synthase of yeast mitochondria. Isolation of subunit j and disruption of the ATP18 gene. J Biol Chem. 1999;274:36-40 pubmed
    ..In the absence of Su j, spontaneously arising rho- cells were observed that lacked also ubiquinol-cytochrome c reductase and cytochrome c oxidase activities. We conclude that Su j is a novel and essential subunit of yeast ATP synthase. ..
  4. Papakonstantinou T, Law R, Nesbitt W, Nagley P, Devenish R. Molecular genetic analysis of the central hydrophobic domain of subunit 8 of yeast mitochondrial ATP synthase. Curr Genet. 1996;30:12-8 pubmed
    ..Encoded by the mitochondrial aap1 gene, Y8 is a 48-amino-acid polypeptide having a central hydrophobic domain (CHD) spanning 19 residues...
  5. Stephens A, Khan M, Roucou X, Nagley P, Devenish R. The molecular neighborhood of subunit 8 of yeast mitochondrial F1F0-ATP synthase probed by cysteine scanning mutagenesis and chemical modification. J Biol Chem. 2003;278:17867-75 pubmed
    ..We conclude that subunit 8 is an integral component of the stator stalk of yeast mitochondrial F(1)F(0)-ATP synthase. ..
  6. Rak M, Tzagoloff A. F1-dependent translation of mitochondrially encoded Atp6p and Atp8p subunits of yeast ATP synthase. Proc Natl Acad Sci U S A. 2009;106:18509-14 pubmed publisher
    ..of F(1), or the Atp11p and Atp12p chaperones that promote F(1) assembly, have normal levels of the bicistronic ATP8/ATP6 mRNAs but fail to synthesize Atp6p and Atp8p...
  7. Förster K, Turina P, Drepper F, Haehnel W, Fischer S, Graber P, et al. Proton transport coupled ATP synthesis by the purified yeast H+ -ATP synthase in proteoliposomes. Biochim Biophys Acta. 2010;1797:1828-37 pubmed publisher
    ..The dependence of the turnover on the phosphate concentration and the dependence of K(M) on pH(out) indicated that the substrate for ATP synthesis is the monoanionic phosphate species H?PO??. ..
  8. Foury F, Roganti T, Lecrenier N, Purnelle B. The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae. FEBS Lett. 1998;440:325-31 pubmed
    ..Our data suggest that shuffling of mobile elements plays an important role in the remodelling of the yeast mitochondrial genome. ..
  9. Macreadie I, Novitski C, Maxwell R, John U, Ooi B, McMullen G, et al. Biogenesis of mitochondria: the mitochondrial gene (aap1) coding for mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae. Nucleic Acids Res. 1983;11:4435-51 pubmed
    A mitochondrial gene (denoted aap1) in Saccharomyces cerevisiae has been characterized by nucleotide sequence analysis of a region of mtDNA between the oxi3 and oli2 genes...