Gene Symbol: ATP16
Description: F1F0 ATP synthase subunit delta
Alias: F1F0 ATP synthase subunit delta
Species: Saccharomyces cerevisiae S288c
Förster K, Turina P, Drepper F, Haehnel W, Fischer S, Graber P, et al
. Proton transport coupled ATP synthesis by the purified yeast H+ -ATP synthase in proteoliposomes. Biochim Biophys Acta. 2010;1797:1828-37 pubmed publisher
..The dependence of the turnover on the phosphate concentration and the dependence of K(M) on pH(out) indicated that the substrate for ATP synthesis is the monoanionic phosphate species H?PO??. ..
Hashimoto T, Yoshida Y, Tagawa K. Binding properties of an intrinsic ATPase inhibitor and occurrence in yeast mitochondria of a protein factor which stabilizes and facilitates the binding of the inhibitor to F1F0-ATPase. J Biochem. 1983;94:715-20 pubmed
..It also efficiently facilitated the binding of the inhibitor to membrane-bound ATPase to form the complex, which reversibly dissociated at slightly alkaline pH. ..
Ichikawa N, Mizuno M. Functional expression of hexahistidine-tagged beta-subunit of yeast F1-ATPase and isolation of the enzyme by immobilized metal affinity chromatography. Protein Expr Purif. 2004;37:97-101 pubmed
..The application of this novel procedure simplifies the number of steps required for the isolation of F1 used for studying the molecular mechanism of catalysis and regulation of the enzyme. ..
Rubinstein J, Dickson V, Runswick M, Walker J. ATP synthase from Saccharomyces cerevisiae: location of subunit h in the peripheral stalk region. J Mol Biol. 2005;345:513-20 pubmed
..Models of the peripheral stalk are proposed that are consistent with this location and with reconstitution experiments conducted with isolated peripheral stalk subunits. ..
Kabaleeswaran V, Puri N, Walker J, Leslie A, Mueller D. Novel features of the rotary catalytic mechanism revealed in the structure of yeast F1 ATPase. EMBO J. 2006;25:5433-42 pubmed publisher
Rak M, Gokova S, Tzagoloff A. Modular assembly of yeast mitochondrial ATP synthase. EMBO J. 2011;30:920-30 pubmed publisher
..These studies show that assembly of the ATP synthase is not a single linear process, as previously thought, but rather involves two separate but coordinately regulated pathways that converge at the end stage. ..
Pagadala V, Vistain L, Symersky J, Mueller D. Characterization of the mitochondrial ATP synthase from yeast Saccharomyces cerevisae. J Bioenerg Biomembr. 2011;43:333-47 pubmed publisher
..F(1)F(o) ATP synthase with ?-GFP was purified to homogeneity and serves as an excellent enzyme for two- and three-dimensional crystallization studies. ..
Giraud M, Velours J. ATP synthase of yeast mitochondria. Isolation of the F1 delta subunit, sequence and disruption of the structural gene. Eur J Biochem. 1994;222:851-9 pubmed
..The double mutant (rho-, ATP delta::URA3) displayed low or no ATPase activity with an unstable catalytic sector, since a polyclonal antibody directed against the beta subunit did not coprecipitate the alpha subunit. ..
Arnold I, Pfeiffer K, Neupert W, Stuart R, Schagger H. ATP synthase of yeast mitochondria. Isolation of subunit j and disruption of the ATP18 gene. J Biol Chem. 1999;274:36-40 pubmed
..In the absence of Su j, spontaneously arising rho- cells were observed that lacked also ubiquinol-cytochrome c reductase and cytochrome c oxidase activities. We conclude that Su j is a novel and essential subunit of yeast ATP synthase. ..