ATP15

Summary

Gene Symbol: ATP15
Description: F1F0 ATP synthase subunit epsilon
Alias: F1F0 ATP synthase subunit epsilon
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Arnold I, Pfeiffer K, Neupert W, Stuart R, Schagger H. ATP synthase of yeast mitochondria. Isolation of subunit j and disruption of the ATP18 gene. J Biol Chem. 1999;274:36-40 pubmed
    ..In the absence of Su j, spontaneously arising rho- cells were observed that lacked also ubiquinol-cytochrome c reductase and cytochrome c oxidase activities. We conclude that Su j is a novel and essential subunit of yeast ATP synthase. ..
  2. Notari L, Arakaki N, Mueller D, Meier S, Amaral J, Becerra S. Pigment epithelium-derived factor binds to cell-surface F(1)-ATP synthase. FEBS J. 2010;277:2192-205 pubmed publisher
    ..They suggest that PEDF-mediated inhibition of ATP synthase may form part of the biochemical mechanisms by which PEDF exerts its antiangiogenic activity. ..
  3. Lau W, Baker L, Rubinstein J. Cryo-EM structure of the yeast ATP synthase. J Mol Biol. 2008;382:1256-64 pubmed publisher
    ..This new map has allowed construction of a model of subunit arrangement in the F(O) motor of ATP synthase that dictates how dimerization of the complex via subunits e and g might occur. ..
  4. Bueler S, Rubinstein J. Location of subunit d in the peripheral stalk of the ATP synthase from Saccharomyces cerevisiae. Biochemistry. 2008;47:11804-10 pubmed publisher
    ..We propose that the C-terminal region of subunit d spans the gap from F 1 to F O, reinforcing this section of the peripheral stalk. ..
  5. Rubinstein J, Dickson V, Runswick M, Walker J. ATP synthase from Saccharomyces cerevisiae: location of subunit h in the peripheral stalk region. J Mol Biol. 2005;345:513-20 pubmed
    ..Models of the peripheral stalk are proposed that are consistent with this location and with reconstitution experiments conducted with isolated peripheral stalk subunits. ..
  6. Kabaleeswaran V, Puri N, Walker J, Leslie A, Mueller D. Novel features of the rotary catalytic mechanism revealed in the structure of yeast F1 ATPase. EMBO J. 2006;25:5433-42 pubmed publisher
  7. Pagadala V, Vistain L, Symersky J, Mueller D. Characterization of the mitochondrial ATP synthase from yeast Saccharomyces cerevisae. J Bioenerg Biomembr. 2011;43:333-47 pubmed publisher
    ..F(1)F(o) ATP synthase with ?-GFP was purified to homogeneity and serves as an excellent enzyme for two- and three-dimensional crystallization studies. ..
  8. Hashimoto T, Yoshida Y, Tagawa K. Binding properties of an intrinsic ATPase inhibitor and occurrence in yeast mitochondria of a protein factor which stabilizes and facilitates the binding of the inhibitor to F1F0-ATPase. J Biochem. 1983;94:715-20 pubmed
    ..It also efficiently facilitated the binding of the inhibitor to membrane-bound ATPase to form the complex, which reversibly dissociated at slightly alkaline pH. ..
  9. Arselin G, Gandar J, Guerin B, Velours J. Isolation and complete amino acid sequence of the mitochondrial ATP synthase epsilon-subunit of the yeast Saccharomyces cerevisiae. J Biol Chem. 1991;266:723-7 pubmed
    ..This polypeptide is rather basic since it contains 7 basic residues and 3 acidic residues. This study shows a slight similarity with the bovine epsilon-subunit ATP synthase since there are 16 identical residues. ..

More Information

Publications14

  1. Guelin E, Chevallier J, Rigoulet M, Guerin B, Velours J. ATP synthase of yeast mitochondria. Isolation and disruption of the ATP epsilon gene. J Biol Chem. 1993;268:161-7 pubmed
    ..The catalytic sector appeared unstable during purification but F0-subunits were still bound to F1. The mutation promoted a highly oligomycin-sensitive uncoupling of the mitochondrial respiration rate. ..
  2. Ichikawa N, Mizuno M. Functional expression of hexahistidine-tagged beta-subunit of yeast F1-ATPase and isolation of the enzyme by immobilized metal affinity chromatography. Protein Expr Purif. 2004;37:97-101 pubmed
    ..The application of this novel procedure simplifies the number of steps required for the isolation of F1 used for studying the molecular mechanism of catalysis and regulation of the enzyme. ..
  3. Jasnos L, Korona R. Epistatic buffering of fitness loss in yeast double deletion strains. Nat Genet. 2007;39:550-4 pubmed
    ..Based on our data and former theoretical work, we suggest that epistasis is likely to diminish the negative effects of mutations when the ability to produce biomass at high rates contributes significantly to fitness. ..
  4. Rak M, Gokova S, Tzagoloff A. Modular assembly of yeast mitochondrial ATP synthase. EMBO J. 2011;30:920-30 pubmed publisher
    ..These studies show that assembly of the ATP synthase is not a single linear process, as previously thought, but rather involves two separate but coordinately regulated pathways that converge at the end stage. ..
  5. Förster K, Turina P, Drepper F, Haehnel W, Fischer S, Graber P, et al. Proton transport coupled ATP synthesis by the purified yeast H+ -ATP synthase in proteoliposomes. Biochim Biophys Acta. 2010;1797:1828-37 pubmed publisher
    ..The dependence of the turnover on the phosphate concentration and the dependence of K(M) on pH(out) indicated that the substrate for ATP synthesis is the monoanionic phosphate species H?PO??. ..