ATG8

Summary

Gene Symbol: ATG8
Description: ubiquitin-like protein ATG8
Alias: APG8, AUT7, CVT5, ubiquitin-like protein ATG8
Species: Saccharomyces cerevisiae S288c
Products:     ATG8

Top Publications

  1. Sawa Makarska J, Abert C, Romanov J, Zens B, Ibiricu I, Martens S. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nat Cell Biol. 2014;16:425-433 pubmed publisher
    ..Selectivity of autophagic processes is mediated by cargo receptors that link cargo to Atg8 family proteins on the developing autophagosomal membrane...
  2. Nakatogawa H, Ishii J, Asai E, Ohsumi Y. Atg4 recycles inappropriately lipidated Atg8 to promote autophagosome biogenesis. Autophagy. 2012;8:177-86 pubmed publisher
    b>Atg8 is a ubiquitin-like protein required for autophagy in the budding yeast Saccharomyces cerevisiae...
  3. Aoki Y, Kanki T, Hirota Y, Kurihara Y, Saigusa T, Uchiumi T, et al. Phosphorylation of Serine 114 on Atg32 mediates mitophagy. Mol Biol Cell. 2011;22:3206-17 pubmed publisher
    ..We also found that Hog1 and Pbs2, which are involved in the osmoregulatory signal transduction cascade, are related to Atg32 phosphorylation and mitophagy. ..
  4. Kirisako T, Baba M, Ishihara N, Miyazawa K, Ohsumi M, Yoshimori T, et al. Formation process of autophagosome is traced with Apg8/Aut7p in yeast. J Cell Biol. 1999;147:435-46 pubmed
    We characterized Apg8/Aut7p essential for autophagy in yeast. Apg8p was transcriptionally upregulated in response to starvation and mostly existed as a protein bound to membrane under both growing and starvation conditions...
  5. Hanada T, Noda N, Satomi Y, Ichimura Y, Fujioka Y, Takao T, et al. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J Biol Chem. 2007;282:37298-302 pubmed
    ..Autophagosome formation requires two ubiquitin-like conjugation systems, the Atg12 and Atg8 systems, which are tightly associated with expansion of autophagosomal membrane...
  6. Yorimitsu T, Klionsky D. Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway. Mol Biol Cell. 2005;16:1593-605 pubmed
    ..These findings suggest that Atg11 plays an integral role in connecting cargo molecules with components of the vesicle-forming machinery. ..
  7. Schwarten M, Stoldt M, Mohrlüder J, Willbold D. Solution structure of Atg8 reveals conformational polymorphism of the N-terminal domain. Biochem Biophys Res Commun. 2010;395:426-31 pubmed publisher
    ..This membrane grows further until its edges fuse to form the double membrane covered autophagosome. Atg8 is a protein, which is required for this initial step of autophagy...
  8. Noda N, Kumeta H, Nakatogawa H, Satoo K, Adachi W, Ishii J, et al. Structural basis of target recognition by Atg8/LC3 during selective autophagy. Genes Cells. 2008;13:1211-8 pubmed publisher
    ..Recent studies have also shed light on another mode of autophagy that selectively degrades various targets. Yeast Atg8 and its mammalian homologue LC3 are ubiquitin-like modifiers that are localized on isolation membranes and play ..
  9. Okamoto K, Kondo Okamoto N, Ohsumi Y. Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy. Dev Cell. 2009;17:87-97 pubmed publisher
    ..In addition, our data suggest that Atg32 interacts with Atg8 and Atg11, autophagy-related proteins critical for recognition of cargo receptors...

More Information

Publications76

  1. Suzuki K, Kondo C, Morimoto M, Ohsumi Y. Selective transport of alpha-mannosidase by autophagic pathways: identification of a novel receptor, Atg34p. J Biol Chem. 2010;285:30019-25 pubmed publisher
    ..Atg34p and Atg19p have similar domain structures and are important for Ams1p transport during autophagy. ..
  2. Ichimura Y, Kirisako T, Takao T, Satomi Y, Shimonishi Y, Ishihara N, et al. A ubiquitin-like system mediates protein lipidation. Nature. 2000;408:488-92 pubmed
    Autophagy is a dynamic membrane phenomenon for bulk protein degradation in the lysosome/vacuole. Apg8/Aut7 is an essential factor for autophagy in yeast...
  3. Sakoh Nakatogawa M, Matoba K, Asai E, Kirisako H, Ishii J, Noda N, et al. Atg12-Atg5 conjugate enhances E2 activity of Atg3 by rearranging its catalytic site. Nat Struct Mol Biol. 2013;20:433-9 pubmed publisher
    ..ubiquitin-like systems have unique features: the E2 enzyme Atg3 conjugates the ubiquitin-like protein Atg8 to the lipid phosphatidylethanolamine, and the other ubiquitin-like protein conjugate Atg12-Atg5 promotes that ..
  4. Novak I, Kirkin V, McEwan D, Zhang J, Wild P, Rozenknop A, et al. Nix is a selective autophagy receptor for mitochondrial clearance. EMBO Rep. 2010;11:45-51 pubmed publisher
    ..Thus, Nix functions as an autophagy receptor, which mediates mitochondrial clearance after mitochondrial damage and during erythrocyte differentiation. ..
  5. Alemu E, Lamark T, Torgersen K, Birgisdottir A, Larsen K, Jain A, et al. ATG8 family proteins act as scaffolds for assembly of the ULK complex: sequence requirements for LC3-interacting region (LIR) motifs. J Biol Chem. 2012;287:39275-90 pubmed publisher
    ..have used pulldown and peptide array overlay assays to study interactions between the ULK complex and six different ATG8 family proteins...
  6. Noda N, Satoo K, Fujioka Y, Kumeta H, Ogura K, Nakatogawa H, et al. Structural basis of Atg8 activation by a homodimeric E1, Atg7. Mol Cell. 2011;44:462-75 pubmed publisher
    ..Atg7, a noncanonical E1, activates two ubiquitin-like proteins, Atg8 and Atg12, and plays a crucial role in autophagy...
  7. Kim J, Huang W, Klionsky D. Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex. J Cell Biol. 2001;152:51-64 pubmed
    ..In both pathways, the vesicle formation process requires the function of the starvation-induced Aut7 protein, which is recruited from the cytosol to the forming Cvt vesicles and autophagosomes...
  8. Farre J, Burkenroad A, Burnett S, Subramani S. Phosphorylation of mitophagy and pexophagy receptors coordinates their interaction with Atg8 and Atg11. EMBO Rep. 2013;14:441-9 pubmed publisher
    ..with two proteins of the core autophagic machinery: the scaffold protein Atg11 and the ubiquitin-like protein Atg8. We found that the Pichia pastoris pexophagy receptor, Atg30, also interacts with Atg8...
  9. Hanada T, Satomi Y, Takao T, Ohsumi Y. The amino-terminal region of Atg3 is essential for association with phosphatidylethanolamine in Atg8 lipidation. FEBS Lett. 2009;583:1078-83 pubmed publisher
    ..Autophagosome formation requires the conjugation of Atg8, a ubiquitin-like protein, to phosphatidylethanolamine (PE)...
  10. Wild P, Farhan H, McEwan D, Wagner S, Rogov V, Brady N, et al. Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth. Science. 2011;333:228-33 pubmed publisher
    ..We propose that phosphorylation of autophagy receptors might be a general mechanism for regulation of cargo-selective autophagy. ..
  11. Xie Z, Nair U, Klionsky D. Atg8 controls phagophore expansion during autophagosome formation. Mol Biol Cell. 2008;19:3290-8 pubmed publisher
    Autophagy is a potent intracellular degradation process with pivotal roles in health and disease. Atg8, a lipid-conjugated ubiquitin-like protein, is required for the formation of autophagosomes, double-membrane vesicles responsible for ..
  12. Kirisako T, Ichimura Y, Okada H, Kabeya Y, Mizushima N, Yoshimori T, et al. The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway. J Cell Biol. 2000;151:263-76 pubmed
    ..Apg8/Aut7, which plays an important role in the formation of such vesicles, tends to bind to membranes in spite of its ..
  13. Suzuki K, Kirisako T, Kamada Y, Mizushima N, Noda T, Ohsumi Y. The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation. EMBO J. 2001;20:5971-81 pubmed
    ..The Apg1p protein kinase complex functions in the late stage of autophagosome formation. Here, we present the classification of Apg proteins into three groups that reflect each step of autophagosome formation. ..
  14. Nakatogawa H, Ichimura Y, Ohsumi Y. Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion. Cell. 2007;130:165-78 pubmed
    ..b>Atg8 is a ubiquitin-like protein required for this process in Saccharomyces cerevisiae that can be conjugated to the ..
  15. Kim J, Huang W, Stromhaug P, Klionsky D. Convergence of multiple autophagy and cytoplasm to vacuole targeting components to a perivacuolar membrane compartment prior to de novo vesicle formation. J Biol Chem. 2002;277:763-73 pubmed
    ..the perivacuolar compartment designates the initial site of membrane binding by the Apg/Cvt vesicle component Aut7, the Cvt cargo receptor Cvt19, and the Apg conjugation machinery, which functions in the de novo formation of ..
  16. Kanki T, Wang K, Baba M, Bartholomew C, Lynch Day M, Du Z, et al. A genomic screen for yeast mutants defective in selective mitochondria autophagy. Mol Biol Cell. 2009;20:4730-8 pubmed publisher
    ..Accordingly, we have named this gene ATG33. The new mutants identified in this analysis will provide a useful foundation for researchers interested in the study of mitochondrial homeostasis and quality control. ..
  17. Kondo Okamoto N, Noda N, Suzuki S, Nakatogawa H, Takahashi I, Matsunami M, et al. Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy. J Biol Chem. 2012;287:10631-8 pubmed publisher
    ..Atg32 interacts with Atg8, a ubiquitin-like protein localized to the autophagosome, and Atg11, a scaffold protein required for selective ..
  18. Ichimura Y, Imamura Y, Emoto K, Umeda M, Noda T, Ohsumi Y. In vivo and in vitro reconstitution of Atg8 conjugation essential for autophagy. J Biol Chem. 2004;279:40584-92 pubmed
    ..In vitro system developed here will provide a powerful system for further understanding the precise role of lipidation and interaction of two ubiquitin-like systems essential for autophagy. ..
  19. Hong S, Kim B, Lee K, Kim S, Jeon H, Kim J, et al. Insights into noncanonical E1 enzyme activation from the structure of autophagic E1 Atg7 with Atg8. Nat Struct Mol Biol. 2011;18:1323-30 pubmed publisher
    ..The autophagic ubiquitin-like (Ubl) molecule Atg8 is activated by the E1-like enzyme Atg7...
  20. Oh oka K, Nakatogawa H, Ohsumi Y. Physiological pH and acidic phospholipids contribute to substrate specificity in lipidation of Atg8. J Biol Chem. 2008;283:21847-52 pubmed publisher
    Yeast Atg8 and its mammalian homolog LC3 are ubiquitin-like proteins involved in autophagy, a primary pathway for degradation of cytosolic constituents in vacuoles/lysosomes...
  21. Lipatova Z, Segev N. A Role for Macro-ER-Phagy in ER Quality Control. PLoS Genet. 2015;11:e1005390 pubmed publisher
  22. Kakuta S, Yamamoto H, Negishi L, Kondo Kakuta C, Hayashi N, Ohsumi Y. Atg9 vesicles recruit vesicle-tethering proteins Trs85 and Ypt1 to the autophagosome formation site. J Biol Chem. 2012;287:44261-9 pubmed publisher
    ..Our data suggest that Atg9 vesicles recruit the TRAPPIII complex and Ypt1 to the preautophagosomal structure. The vesicle-tethering machinery consequently acts in the process of autophagosome formation. ..
  23. Krick R, Bremer S, Welter E, Schlotterhose P, Muehe Y, Eskelinen E, et al. Cdc48/p97 and Shp1/p47 regulate autophagosome biogenesis in concert with ubiquitin-like Atg8. J Cell Biol. 2010;190:965-73 pubmed publisher
    ..system for autophagosome biogenesis but detect interaction of Shp1 with the ubiquitin-fold autophagy protein Atg8. Atg8 coupled to phosphatidylethanolamine (PE) is crucial for autophagosome elongation and, in vitro, mediates ..
  24. Yamazaki Sato H, Tanida I, Ueno T, Kominami E. The carboxyl terminal 17 amino acids within Apg7 are essential for Apg8 lipidation, but not for Apg12 conjugation. FEBS Lett. 2003;551:71-7 pubmed
    In the yeast, Saccharomyces cerevisiae, two ubiquitin-like modifications, Apg12 conjugation with Apg5 and Apg8 lipidation with phosphatidylethanolamine, are essential for autophagy and the cytoplasm-to-vacuole transport of aminopeptidase ..
  25. Lu K, Psakhye I, Jentsch S. Autophagic clearance of polyQ proteins mediated by ubiquitin-Atg8 adaptors of the conserved CUET protein family. Cell. 2014;158:549-63 pubmed publisher
    ..autophagosomes involving adaptors like human p62 that bind ubiquitin and the autophagosomal ubiquitin-like protein Atg8/LC3; however, whether similar pathways exist in lower eukaryotes remained unclear...
  26. Eiyama A, Okamoto K. Protein N-terminal Acetylation by the NatA Complex Is Critical for Selective Mitochondrial Degradation. J Biol Chem. 2015;290:25034-44 pubmed publisher
    ..Together, our data implicate NatA-mediated protein modification as an early regulatory step crucial for efficient mitophagy. ..
  27. Yu Z, Ni T, Hong B, Wang H, Jiang F, Zou S, et al. Dual roles of Atg8-PE deconjugation by Atg4 in autophagy. Autophagy. 2012;8:883-92 pubmed publisher
    ..Little is known, however, about the physiological function of the reverse reaction, deconjugation. Atg8 is a unique Ubl protein whose conjugation target is the lipid phosphatidylethanolamine (PE)...
  28. Shen Z, Li Y, Gasparski A, Abeliovich H, Greenberg M. Cardiolipin Regulates Mitophagy through the Protein Kinase C Pathway. J Biol Chem. 2017;292:2916-2923 pubmed publisher
    ..Mutants of autophagy/mitophagy genes ATG8, ATG18, and ATG32 synthetically interact with CL synthase mutant crd1Δ...
  29. Amar N, Lustig G, Ichimura Y, Ohsumi Y, Elazar Z. Two newly identified sites in the ubiquitin-like protein Atg8 are essential for autophagy. EMBO Rep. 2006;7:635-42 pubmed
    b>Atg8, a member of a novel ubiquitin-like protein family, is an essential component of the autophagic machinery in yeast...
  30. Sánchez Wandelmer J, Kriegenburg F, Rohringer S, Schuschnig M, Gómez Sánchez R, Zens B, et al. Atg4 proteolytic activity can be inhibited by Atg1 phosphorylation. Nat Commun. 2017;8:295 pubmed publisher
    The biogenesis of autophagosomes depends on the conjugation of Atg8-like proteins with phosphatidylethanolamine...
  31. Sakoh Nakatogawa M, Kirisako H, Nakatogawa H, Ohsumi Y. Localization of Atg3 to autophagy-related membranes and its enhancement by the Atg8-family interacting motif to promote expansion of the membranes. FEBS Lett. 2015;589:744-9 pubmed publisher
    The E2 enzyme Atg3 conjugates the ubiquitin-like protein Atg8 to phosphatidylethanolamine (PE) to drive autophagosome formation in Saccharomyces cerevisiae...
  32. Marshall R, McLoughlin F, Vierstra R. Autophagic Turnover of Inactive 26S Proteasomes in Yeast Is Directed by the Ubiquitin Receptor Cue5 and the Hsp42 Chaperone. Cell Rep. 2016;16:1717-1732 pubmed publisher
    ..Whereas the core autophagic machineries required for Atg8 lipidation and vesiculation are essential for both routes, the upstream Atg1 kinase participates only in starvation-..
  33. Abert C, Kontaxis G, Martens S. Accessory Interaction Motifs in the Atg19 Cargo Receptor Enable Strong Binding to the Clustered Ubiquitin-related Atg8 Protein. J Biol Chem. 2016;291:18799-808 pubmed publisher
    ..link the cargo to autophagosomal membranes because of their ability to simultaneously interact with the cargo and Atg8 proteins that coat the membrane...
  34. Suzuki K, Akioka M, Kondo Kakuta C, Yamamoto H, Ohsumi Y. Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae. J Cell Sci. 2013;126:2534-44 pubmed publisher
    ..By contrast, Atg1, Atg8 and the Atg16-Atg12-Atg5 complex were present at both the VICS and the cup-shaped IM...
  35. Fracchiolla D, Sawa Makarska J, Zens B, Ruiter A, Zaffagnini G, Brezovich A, et al. Mechanism of cargo-directed Atg8 conjugation during selective autophagy. elife. 2016;5: pubmed publisher
    ..autophagy is mediated by cargo receptors that link the cargo to the isolation membrane via interactions with Atg8 proteins...
  36. Kraft C, Kijanska M, Kalie E, Siergiejuk E, Lee S, Semplicio G, et al. Binding of the Atg1/ULK1 kinase to the ubiquitin-like protein Atg8 regulates autophagy. EMBO J. 2012;31:3691-703 pubmed publisher
    ..We identify Atg8 as a novel regulator of Atg1/ULK1, which directly binds Atg1/ULK1 in a LC3-interaction region (LIR)-dependent ..
  37. Mochida K, Oikawa Y, Kimura Y, Kirisako H, Hirano H, Ohsumi Y, et al. Receptor-mediated selective autophagy degrades the endoplasmic reticulum and the nucleus. Nature. 2015;522:359-62 pubmed publisher
    ..Our results provide fundamental insight into the pathophysiological roles and mechanisms of 'ER-phagy' and 'nucleophagy' in other organisms. ..
  38. Abreu S, Kriegenburg F, Gómez Sánchez R, Mari M, Sánchez Wandelmer J, Skytte Rasmussen M, et al. Conserved Atg8 recognition sites mediate Atg4 association with autophagosomal membranes and Atg8 deconjugation. EMBO Rep. 2017;18:765-780 pubmed publisher
    Deconjugation of the Atg8/LC3 protein family members from phosphatidylethanolamine (PE) by Atg4 proteases is essential for autophagy progression, but how this event is regulated remains to be understood...
  39. Skytte Rasmussen M, Mouilleron S, Kumar Shrestha B, Wirth M, Lee R, Bowitz Larsen K, et al. ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8. Autophagy. 2017;13:834-853 pubmed publisher
    ..residues of the inactive proform of proteins of the ortholog and paralog LC3 and GABARAP subfamilies of yeast Atg8 to expose a C-terminal glycine that is conjugated to phosphatidylethanolamine during autophagosome formation...
  40. Lemus L, Ribas J, Sikorska N, Goder V. An ER-Localized SNARE Protein Is Exported in Specific COPII Vesicles for Autophagosome Biogenesis. Cell Rep. 2016;14:1710-1722 pubmed publisher
    ..Ufe1 is increasingly exported from the ER and targeted to intracellular sites that contain the autophagy markers Atg8 and Atg9. In addition, Ufe1 interacts with non-ER SNARE proteins implicated in autophagosome formation...
  41. Komatsu M, Tanida I, Ueno T, Ohsumi M, Ohsumi Y, Kominami E. The C-terminal region of an Apg7p/Cvt2p is required for homodimerization and is essential for its E1 activity and E1-E2 complex formation. J Biol Chem. 2001;276:9846-54 pubmed
    ..These results suggest that Apg7p forms a homodimer via the C-terminal region and that the C-terminal region is essential for both the activity of the E1 enzyme for Apg12p and Apg8p as well as the formation of an E1-E2 complex for Apg8p. ..
  42. Bicknell A, Tourtellotte J, Niwa M. Late phase of the endoplasmic reticulum stress response pathway is regulated by Hog1 MAP kinase. J Biol Chem. 2010;285:17545-55 pubmed publisher
    ..From its cytoplasmic location, Hog1 contributes to the activation of autophagy by enhancing the stability of Atg8, a critical autophagy protein. Thus, Hog1 coordinates a multifaceted response to persistent ER stress.
  43. Sakakibara K, Eiyama A, Suzuki S, Sakoh Nakatogawa M, Okumura N, Tani M, et al. Phospholipid methylation controls Atg32-mediated mitophagy and Atg8 recycling. EMBO J. 2015;34:2703-19 pubmed publisher
    ..loss of Opi3 results in accumulation of phosphatidylmonomethylethanolamine (PMME) and, surprisingly, generation of Atg8-PMME, a mitophagy-incompetent lipid conjugate of the autophagy-related ubiquitin-like modifier...
  44. Pérez Pérez M, Zaffagnini M, Marchand C, Crespo J, Lemaire S. The yeast autophagy protease Atg4 is regulated by thioredoxin. Autophagy. 2014;10:1953-64 pubmed publisher
    ..regulation of autophagosome biogenesis, since mutation of these cysteines resulted in increased recruitment of Atg8 to the phagophore assembly site...
  45. Maeda Y, Oku M, Sakai Y. A defect of the vacuolar putative lipase Atg15 accelerates degradation of lipid droplets through lipolysis. Autophagy. 2015;11:1247-58 pubmed publisher
    ..Our data provide evidence for a novel link between autophagic flux and LD dynamics integrated with Atg15 activity. ..
  46. Zens B, Sawa Makarska J, Martens S. In vitro systems for Atg8 lipidation. Methods. 2015;75:37-43 pubmed publisher
    ..mediate the conjugation of the ubiquitin-like Atg12 to the Atg5 protein and of the ubiquitin-like protein Atg8 to the headgroup of the membrane lipid phosphatidylethanolamine...
  47. Ho K, Chang H, Huang W. Mutation at the cargo-receptor binding site of Atg8 also affects its general autophagy regulation function. Autophagy. 2009;5:461-71 pubmed
    ..Among those characterized autophagy regulators, the ubiquitin-like protein Atg8 is found to be a membrane modifier that both regulates biogenesis of transport vesicles and interacts with the ..
  48. Yamada Y, Suzuki N, Hanada T, Ichimura Y, Kumeta H, Fujioka Y, et al. The crystal structure of Atg3, an autophagy-related ubiquitin carrier protein (E2) enzyme that mediates Atg8 lipidation. J Biol Chem. 2007;282:8036-43 pubmed
    Atg3 is an E2-like enzyme that catalyzes the conjugation of Atg8 and phosphatidylethanolamine (PE)...
  49. Yi C, Ma M, Ran L, Zheng J, Tong J, Zhu J, et al. Function and molecular mechanism of acetylation in autophagy regulation. Science. 2012;336:474-7 pubmed publisher
    ..Specifically, acetylation of K19 and K48 of Atg3 regulated autophagy by controlling Atg3 and Atg8 interaction and lipidation of Atg8...
  50. Kario E, Amar N, Elazar Z, Navon A. A new autophagy-related checkpoint in the degradation of an ERAD-M target. J Biol Chem. 2011;286:11479-91 pubmed publisher
    ..lesion, associated with the accumulation of the yeast ERAD-M substrate 6Myc-Hmg2p elicits the recruitment of Atg8 and elements of the cytosol to vacuole targeting (CVT) to the membrane, leading to attenuation in the degradation ..
  51. Ghavidel A, Baxi K, Ignatchenko V, Prusinkiewicz M, Arnason T, Kislinger T, et al. A Genome Scale Screen for Mutants with Delayed Exit from Mitosis: Ire1-Independent Induction of Autophagy Integrates ER Homeostasis into Mitotic Lifespan. PLoS Genet. 2015;11:e1005429 pubmed publisher
    ..Our data provide evidence that catabolism of protein aggregates, a natural byproduct of high protein synthesis and turn over in dividing cells, is among the drivers of mitotic longevity in eukaryotes. ..
  52. Legesse Miller A, Sagiv Y, Glozman R, Elazar Z. Aut7p, a soluble autophagic factor, participates in multiple membrane trafficking processes. J Biol Chem. 2000;275:32966-73 pubmed
    ..Aut7p largely substitutes for the activity of GATE-16 in mammalian intra-Golgi transport in vitro. In vivo, AUT7 interacts genetically with endoplasmic reticulum to Golgi SNAREs, specifically with BET1 and SEC22...
  53. Popovic D, Akutsu M, Novak I, Harper J, Behrends C, Dikic I. Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers. Mol Cell Biol. 2012;32:1733-44 pubmed publisher
    ..autophagosomal membranes to act selectively toward specific cargo is dependent on the small ubiquitin-like modifier ATG8/LC3 and the LC3-interacting region (LIR) present in autophagy receptors...
  54. Jotwani A, Richerson D, Motta I, Julca Zevallos O, Melia T. Approaches to the study of Atg8-mediated membrane dynamics in vitro. Methods Cell Biol. 2012;108:93-116 pubmed publisher
    ..One factor implicated in both membrane expansion and membrane fusion is the ubiquitin-like protein, Atg8. During autophagy, Atg8 becomes covalently bound to phosphatidylethanolamine (PE) on the pre-autophagosomal ..
  55. Bartholomew C, Suzuki T, Du Z, Backues S, Jin M, Lynch Day M, et al. Ume6 transcription factor is part of a signaling cascade that regulates autophagy. Proc Natl Acad Sci U S A. 2012;109:11206-10 pubmed publisher
    ..Following autophagy induction, Atg8 shows the greatest change in expression of any of the proteins required for autophagy...
  56. Morales Quinones M, Winston J, Stromhaug P. Propeptide of aminopeptidase 1 protein mediates aggregation and vesicle formation in cytoplasm-to-vacuole targeting pathway. J Biol Chem. 2012;287:10121-33 pubmed publisher
    ..We show that Ape1 aggregates bind Atg19 and Atg8 in vitro; this could be used as a scaffold for an in vitro assay of autophagosome formation to elucidate the ..
  57. Juris L, Montino M, Rube P, Schlotterhose P, Thumm M, Krick R. PI3P binding by Atg21 organises Atg8 lipidation. EMBO J. 2015;34:955-73 pubmed publisher
    Autophagosome biogenesis requires two ubiquitin-like conjugation systems. One couples ubiquitin-like Atg8 to phosphatidylethanolamine, and the other couples ubiquitin-like Atg12 to Atg5. Atg12~Atg5 then forms a heterodimer with Atg16...
  58. Nemec A, Howell L, Peterson A, Murray M, Tomko R. Autophagic clearance of proteasomes in yeast requires the conserved sorting nexin Snx4. J Biol Chem. 2017;292:21466-21480 pubmed publisher
  59. Lang T, Schaeffeler E, Bernreuther D, Bredschneider M, Wolf D, Thumm M. Aut2p and Aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole. EMBO J. 1998;17:3597-607 pubmed
    AUT2 and AUT7, two novel genes essential for autophagocytosis in the yeast Saccharomyces cerevisiae were isolated. AUT7 was identified as a low copy suppressor of autophagic defects in aut2-1 cells...
  60. Nakatogawa H, Ohbayashi S, Sakoh Nakatogawa M, Kakuta S, Suzuki S, Kirisako H, et al. The autophagy-related protein kinase Atg1 interacts with the ubiquitin-like protein Atg8 via the Atg8 family interacting motif to facilitate autophagosome formation. J Biol Chem. 2012;287:28503-7 pubmed publisher
    ..The formation of the autophagosome requires autophagy-related (Atg) proteins. Atg8 is a ubiquitin-like protein that localizes to the isolation membrane; a subpopulation of this protein remains ..
  61. Gao J, Langemeyer L, Kümmel D, Reggiori F, Ungermann C. Molecular mechanism to target the endosomal Mon1-Ccz1 GEF complex to the pre-autophagosomal structure. elife. 2018;7: pubmed publisher
    ..We find that Mon1-Ccz1 directly binds to Atg8, the yeast homolog of the members of the mammalian LC3 protein family...
  62. Cao Y, Klionsky D. New insights into autophagy using a multiple knockout strain. Autophagy. 2008;4:1073-5 pubmed
    ..In this addendum, we briefly discuss our findings and consider fields where this multiple knockout strain can be of potential use. ..
  63. Maeda Y, Oku M, Sakai Y. Autophagy-independent function of Atg8 in lipid droplet dynamics in yeast. J Biochem. 2017;161:339-348 pubmed publisher
    ..b>Atg8 protein in the yeast Saccharomyces cerevisiae is recruited to membrane structures synthesized during autophagy via ..
  64. Hirata E, Ohya Y, Suzuki K. Atg4 plays an important role in efficient expansion of autophagic isolation membranes by cleaving lipidated Atg8 in Saccharomyces cerevisiae. PLoS ONE. 2017;12:e0181047 pubmed publisher
    ..In S. cerevisiae, the ubiquitin-like protein Atg8 is C-terminally conjugated to the phospholipid phosphatidylethanolamine (PE) to generate Atg8-PE...
  65. Shpilka T, Welter E, Borovsky N, Amar N, Shimron F, Peleg Y, et al. Fatty acid synthase is preferentially degraded by autophagy upon nitrogen starvation in yeast. Proc Natl Acad Sci U S A. 2015;112:1434-9 pubmed publisher
    ..Here we describe a novel interaction between autophagy-related protein 8 (Atg8) and fatty acid synthase (FAS), a pivotal enzymatic complex responsible for the entire synthesis of C16- and C18-..
  66. Mijaljica D, Prescott M, Devenish R. A late form of nucleophagy in Saccharomyces cerevisiae. PLoS ONE. 2012;7:e40013 pubmed publisher
    ..Moreover, the inhibition of LN in some mutants is accompanied by alterations in nuclear morphology. ..
  67. Meiling Wesse K, Epple U, Krick R, Barth H, Appelles A, Voss C, et al. Trs85 (Gsg1), a component of the TRAPP complexes, is required for the organization of the preautophagosomal structure during selective autophagy via the Cvt pathway. J Biol Chem. 2005;280:33669-78 pubmed
    ..I is normally recruited to the preautophagosomal structure, the recruitment of green fluorescent protein-Atg8 depends on Trs85. Autophagy proceeds in the absence of Trs85, albeit at a reduced rate...