ATG12

Summary

Gene Symbol: ATG12
Description: Atg12p
Alias: APG12, Atg12p
Species: Saccharomyces cerevisiae S288c
Products:     ATG12

Top Publications

  1. Romanov J, Walczak M, Ibiricu I, Schüchner S, Ogris E, Kraft C, et al. Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation. EMBO J. 2012;31:4304-17 pubmed publisher
    ..The conserved Atg5-Atg12/Atg16 complex is essential for autophagosome formation...
  2. Mizushima N, Noda T, Yoshimori T, Tanaka Y, Ishii T, George M, et al. A protein conjugation system essential for autophagy. Nature. 1998;395:395-8 pubmed
    ..The carboxy-terminal glycine residue of Apg12, a 186-amino-acid protein, is conjugated to a lysine at residue 149 of Apg5, a 294-amino-acid protein...
  3. Tanida I, Mizushima N, Kiyooka M, Ohsumi M, Ueno T, Ohsumi Y, et al. Apg7p/Cvt2p: A novel protein-activating enzyme essential for autophagy. Mol Biol Cell. 1999;10:1367-79 pubmed
    ..These results indicated that Apg7p functions as a novel protein-activating enzyme necessary for Apg12p-Apg5p conjugation. ..
  4. Mizushima N, Noda T, Ohsumi Y. Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway. EMBO J. 1999;18:3888-96 pubmed
    ..These results suggest that the Apg12p-Apg5p conjugate requires Apg16p to accomplish its role in the autophagy pathway, and Apg16p is a key molecule as a linker to form the Apg12p-Apg5p-Apg16p multimer. ..
  5. Shintani T, Mizushima N, Ogawa Y, Matsuura A, Noda T, Ohsumi Y. Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast. EMBO J. 1999;18:5234-41 pubmed
    ..These findings indicate that Apg10p is a new type of protein-conjugating enzyme that functions in the Apg12p-Apg5p conjugation pathway. ..
  6. Nakatogawa H, Ishii J, Asai E, Ohsumi Y. Atg4 recycles inappropriately lipidated Atg8 to promote autophagosome biogenesis. Autophagy. 2012;8:177-86 pubmed publisher
  7. Kim J, Dalton V, Eggerton K, Scott S, Klionsky D. Apg7p/Cvt2p is required for the cytoplasm-to-vacuole targeting, macroautophagy, and peroxisome degradation pathways. Mol Biol Cell. 1999;10:1337-51 pubmed
  8. Kim J, Huang W, Klionsky D. Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex. J Cell Biol. 2001;152:51-64 pubmed
    ..These findings define a series of steps that results in the modification of Aut7p and its subsequent binding to the sequestering transport vesicles of the autophagy and cytoplasm to vacuole targeting pathways. ..
  9. Sakoh Nakatogawa M, Matoba K, Asai E, Kirisako H, Ishii J, Noda N, et al. Atg12-Atg5 conjugate enhances E2 activity of Atg3 by rearranging its catalytic site. Nat Struct Mol Biol. 2013;20:433-9 pubmed publisher
    ..ubiquitin-like protein Atg8 to the lipid phosphatidylethanolamine, and the other ubiquitin-like protein conjugate Atg12-Atg5 promotes that conjugase activity of Atg3...

More Information

Publications24

  1. Hanada T, Noda N, Satomi Y, Ichimura Y, Fujioka Y, Takao T, et al. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J Biol Chem. 2007;282:37298-302 pubmed
    ..Autophagosome formation requires two ubiquitin-like conjugation systems, the Atg12 and Atg8 systems, which are tightly associated with expansion of autophagosomal membrane...
  2. Kaufmann A, Beier V, Franquelim H, Wollert T. Molecular mechanism of autophagic membrane-scaffold assembly and disassembly. Cell. 2014;156:469-81 pubmed publisher
    ..Atg8 to phosphatidylethanolamine (Atg8-PE) in autophagic membranes by a complex consisting of Atg16 and the Atg12-Atg5 conjugate...
  3. Yen W, Shintani T, Nair U, Cao Y, Richardson B, Li Z, et al. The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy. J Cell Biol. 2010;188:101-14 pubmed publisher
    ..In addition, mutations in the COG genes resulted in the mislocalization of Atg8 and Atg9, which are critical components involved in autophagosome formation...
  4. Komatsu M, Tanida I, Ueno T, Ohsumi M, Ohsumi Y, Kominami E. The C-terminal region of an Apg7p/Cvt2p is required for homodimerization and is essential for its E1 activity and E1-E2 complex formation. J Biol Chem. 2001;276:9846-54 pubmed
    ..These results suggest that Apg7p forms a homodimer via the C-terminal region and that the C-terminal region is essential for both the activity of the E1 enzyme for Apg12p and Apg8p as well as the formation of an E1-E2 complex for Apg8p. ..
  5. Kanki T, Wang K, Baba M, Bartholomew C, Lynch Day M, Du Z, et al. A genomic screen for yeast mutants defective in selective mitochondria autophagy. Mol Biol Cell. 2009;20:4730-8 pubmed publisher
    ..Accordingly, we have named this gene ATG33. The new mutants identified in this analysis will provide a useful foundation for researchers interested in the study of mitochondrial homeostasis and quality control. ..
  6. Hanada T, Ohsumi Y. Structure-function relationship of Atg12, a ubiquitin-like modifier essential for autophagy. Autophagy. 2005;1:110-8 pubmed
    b>Atg12, a post-translational modifier, is activated and conjugated to Atg5 by a ubiquitin-like conjugation system, though it has no obvious sequence homology to ubiquitin...
  7. Yamazaki Sato H, Tanida I, Ueno T, Kominami E. The carboxyl terminal 17 amino acids within Apg7 are essential for Apg8 lipidation, but not for Apg12 conjugation. FEBS Lett. 2003;551:71-7 pubmed
    In the yeast, Saccharomyces cerevisiae, two ubiquitin-like modifications, Apg12 conjugation with Apg5 and Apg8 lipidation with phosphatidylethanolamine, are essential for autophagy and the cytoplasm-to-vacuole transport of aminopeptidase ..
  8. Suzuki K, Akioka M, Kondo Kakuta C, Yamamoto H, Ohsumi Y. Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae. J Cell Sci. 2013;126:2534-44 pubmed publisher
    ..By contrast, Atg1, Atg8 and the Atg16-Atg12-Atg5 complex were present at both the VICS and the cup-shaped IM. We designate this localization the 'IM' pattern...
  9. Maeda Y, Oku M, Sakai Y. A defect of the vacuolar putative lipase Atg15 accelerates degradation of lipid droplets through lipolysis. Autophagy. 2015;11:1247-58 pubmed publisher
    ..Our data provide evidence for a novel link between autophagic flux and LD dynamics integrated with Atg15 activity. ..
  10. Mijaljica D, Prescott M, Devenish R. A late form of nucleophagy in Saccharomyces cerevisiae. PLoS ONE. 2012;7:e40013 pubmed publisher
    ..Moreover, the inhibition of LN in some mutants is accompanied by alterations in nuclear morphology. ..
  11. Noda N, Fujioka Y, Hanada T, Ohsumi Y, Inagaki F. Structure of the Atg12-Atg5 conjugate reveals a platform for stimulating Atg8-PE conjugation. EMBO Rep. 2013;14:206-11 pubmed publisher
    b>Atg12 is conjugated to Atg5 through enzymatic reactions similar to ubiquitination...
  12. Millson S, van Oosten Hawle P, Alkuriji M, Truman A, Siderius M, Piper P. Cdc37 engages in stable, S14A mutation-reinforced association with the most atypical member of the yeast kinome, Cdk-activating kinase (Cak1). Cell Stress Chaperones. 2014;19:695-703 pubmed publisher
    ..Thus, this is the first Cdc37 client kinase found to be excluded from S14 phosphorylation-dependent interaction. The unusual stability of this Cak1:Cdc37 association may partly reflect unique structural features of the fungal Cak1. ..
  13. Zens B, Sawa Makarska J, Martens S. In vitro systems for Atg8 lipidation. Methods. 2015;75:37-43 pubmed publisher
    ..of two ubiquitin-like conjugation reactions that collectively mediate the conjugation of the ubiquitin-like Atg12 to the Atg5 protein and of the ubiquitin-like protein Atg8 to the headgroup of the membrane lipid ..
  14. Fracchiolla D, Sawa Makarska J, Zens B, Ruiter A, Zaffagnini G, Brezovich A, et al. Mechanism of cargo-directed Atg8 conjugation during selective autophagy. elife. 2016;5: pubmed publisher
    ..Atg19, Atg34 and the human p62, Optineurin and NDP52 cargo receptors interact with the E3-like enzyme Atg12~Atg5-Atg16, which stimulates Atg8 conjugation...
  15. Kuma A, Mizushima N, Ishihara N, Ohsumi Y. Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast. J Biol Chem. 2002;277:18619-25 pubmed
    ..This process requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg5...