ATG11

Summary

Gene Symbol: ATG11
Description: autophagy protein ATG11
Alias: CVT3, CVT9, autophagy protein ATG11
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kim J, Kamada Y, Stromhaug P, Guan J, Hefner Gravink A, Baba M, et al. Cvt9/Gsa9 functions in sequestering selective cytosolic cargo destined for the vacuole. J Cell Biol. 2001;153:381-96 pubmed
    ..We have identified Cvt9 and its Pichia pastoris counterpart Gsa9. In S...
  2. Yorimitsu T, Klionsky D. Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway. Mol Biol Cell. 2005;16:1593-605 pubmed
    ..to the pre-autophagosomal structure (PAS), the putative site of Cvt vesicle formation, in a process dependent on Atg11. Here, we show that this interaction occurs through the Atg11 C terminus; subsequent recruitment of the Cvt ..
  3. Aoki Y, Kanki T, Hirota Y, Kurihara Y, Saigusa T, Uchiumi T, et al. Phosphorylation of Serine 114 on Atg32 mediates mitophagy. Mol Biol Cell. 2011;22:3206-17 pubmed publisher
    ..When mitophagy is induced in yeast, mitochondrial residential protein Atg32 binds Atg11, an adaptor protein for selective types of autophagy, and it is recruited into the vacuole along with mitochondria...
  4. Kamada Y, Funakoshi T, Shintani T, Nagano K, Ohsumi M, Ohsumi Y. Tor-mediated induction of autophagy via an Apg1 protein kinase complex. J Cell Biol. 2000;150:1507-13 pubmed
    ..Finally, other Apg1-associating proteins, such as Apg17 and Cvt9, are shown to function specifically in autophagy or the Cvt pathway, respectively, suggesting that the Apg1 complex ..
  5. Kondo Okamoto N, Noda N, Suzuki S, Nakatogawa H, Takahashi I, Matsunami M, et al. Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy. J Biol Chem. 2012;287:10631-8 pubmed publisher
    ..Atg32 interacts with Atg8, a ubiquitin-like protein localized to the autophagosome, and Atg11, a scaffold protein required for selective autophagy-related pathways, although the significance of these ..
  6. Kawamata T, Kamada Y, Kabeya Y, Sekito T, Ohsumi Y. Organization of the pre-autophagosomal structure responsible for autophagosome formation. Mol Biol Cell. 2008;19:2039-50 pubmed publisher
    ..Our results suggest that Atg1 complex and the autophagy-unique Atg proteins cooperatively organize the PAS in response to starvation signals. ..
  7. Kanki T, Klionsky D. Mitophagy in yeast occurs through a selective mechanism. J Biol Chem. 2008;283:32386-93 pubmed publisher
    ..Using this method, we screened several atg mutants and found that ATG11, a gene that is essential only for selective autophagy, is also essential for mitophagy...
  8. Suzuki K, Kubota Y, Sekito T, Ohsumi Y. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes Cells. 2007;12:209-18 pubmed
  9. Suzuki K, Kondo C, Morimoto M, Ohsumi Y. Selective transport of alpha-mannosidase by autophagic pathways: identification of a novel receptor, Atg34p. J Biol Chem. 2010;285:30019-25 pubmed publisher
    ..Atg34p and Atg19p have similar domain structures and are important for Ams1p transport during autophagy. ..

More Information

Publications43

  1. Kanki T, Wang K, Baba M, Bartholomew C, Lynch Day M, Du Z, et al. A genomic screen for yeast mutants defective in selective mitochondria autophagy. Mol Biol Cell. 2009;20:4730-8 pubmed publisher
    ..Accordingly, we have named this gene ATG33. The new mutants identified in this analysis will provide a useful foundation for researchers interested in the study of mitochondrial homeostasis and quality control. ..
  2. Lipatova Z, Belogortseva N, Zhang X, Kim J, Taussig D, Segev N. Regulation of selective autophagy onset by a Ypt/Rab GTPase module. Proc Natl Acad Sci U S A. 2012;109:6981-6 pubmed publisher
    ..Here, we identify Atg11, the preautophagosomal structure (PAS) organizer, as a downstream effector of Ypt1 and show that the Ypt1-Atg11 ..
  3. Farre J, Burkenroad A, Burnett S, Subramani S. Phosphorylation of mitophagy and pexophagy receptors coordinates their interaction with Atg8 and Atg11. EMBO Rep. 2013;14:441-9 pubmed publisher
    ..receptors Atg19 and Atg32 interact with two proteins of the core autophagic machinery: the scaffold protein Atg11 and the ubiquitin-like protein Atg8...
  4. Suzuki K, Morimoto M, Kondo C, Ohsumi Y. Selective autophagy regulates insertional mutagenesis by the Ty1 retrotransposon in Saccharomyces cerevisiae. Dev Cell. 2011;21:358-65 pubmed publisher
    ..We propose that selective autophagy safeguards genome integrity against excessive insertional mutagenesis caused during nutrient starvation by transposable elements in eukaryotic cells. ..
  5. Cheong H, Nair U, Geng J, Klionsky D. The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae. Mol Biol Cell. 2008;19:668-81 pubmed
    ..Therefore, appropriate recruitment of Atg proteins to this site is critical for their function in autophagy. Atg11 facilitates PAS recruitment for the cytoplasm-to-vacuole targeting pathway, which is a specific, autophagy-like ..
  6. Okamoto K, Kondo Okamoto N, Ohsumi Y. Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy. Dev Cell. 2009;17:87-97 pubmed publisher
    ..In addition, our data suggest that Atg32 interacts with Atg8 and Atg11, autophagy-related proteins critical for recognition of cargo receptors...
  7. Monastyrska I, Shintani T, Klionsky D, Reggiori F. Atg11 directs autophagosome cargoes to the PAS along actin cables. Autophagy. 2006;2:119-21 pubmed
    ..3) In this addendum, we discuss the possible central role of Atg11 as a molecule connecting cargoes, actin and pre-utophagosomal structure (PAS) elements.
  8. Mochida K, Ohsumi Y, Nakatogawa H. Hrr25 phosphorylates the autophagic receptor Atg34 to promote vacuolar transport of α-mannosidase under nitrogen starvation conditions. FEBS Lett. 2014;588:3862-9 pubmed publisher
    ..does not affect the interaction of Atg34 with Ams1, but instead promotes Atg34 binding to the adaptor protein Atg11, which recruits the autophagy machinery to the Ams1-Atg34 complex, resulting in activation of the vacuolar ..
  9. Sawa Makarska J, Abert C, Romanov J, Zens B, Ibiricu I, Martens S. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nat Cell Biol. 2014;16:425-433 pubmed publisher
    ..These properties are essential for the function of Atg19 during selective autophagy in vivo. Our results suggest that cargo receptors contribute to tight membrane bending of the isolation membrane around the cargo. ..
  10. Shin C, Huh W. Bidirectional regulation between TORC1 and autophagy in Saccharomyces cerevisiae. Autophagy. 2011;7:854-62 pubmed
    ..Our data suggest that TORC1 can be substantially, if not fully, reactivated in an autophagy-dependent manner under ongoing starvation, and that partially reactivated TORC1 eventually plays a role in the attenuation of autophagy. ..
  11. Morales Quinones M, Winston J, Stromhaug P. Propeptide of aminopeptidase 1 protein mediates aggregation and vesicle formation in cytoplasm-to-vacuole targeting pathway. J Biol Chem. 2012;287:10121-33 pubmed publisher
    ..We show that Ape1 aggregates bind Atg19 and Atg8 in vitro; this could be used as a scaffold for an in vitro assay of autophagosome formation to elucidate the mechanisms of autophagy. ..
  12. Eiyama A, Okamoto K. Protein N-terminal Acetylation by the NatA Complex Is Critical for Selective Mitochondrial Degradation. J Biol Chem. 2015;290:25034-44 pubmed publisher
    ..Together, our data implicate NatA-mediated protein modification as an early regulatory step crucial for efficient mitophagy. ..
  13. Yen W, Legakis J, Nair U, Klionsky D. Atg27 is required for autophagy-dependent cycling of Atg9. Mol Biol Cell. 2007;18:581-93 pubmed
    ..These data support a hypothesis that multiple membrane sources supply the lipids needed for autophagosome formation. ..
  14. Mao K, Wang K, Liu X, Klionsky D. The scaffold protein Atg11 recruits fission machinery to drive selective mitochondria degradation by autophagy. Dev Cell. 2013;26:9-18 pubmed publisher
    ..is induced, the fission complex is recruited to the degrading mitochondria through an interaction between Atg11 and Dnm1; interfering with this interaction severely blocks mitophagy...
  15. Yokota H, Gomi K, Shintani T. Induction of autophagy by phosphate starvation in an Atg11-dependent manner in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2017;483:522-527 pubmed publisher
    ..Phosphate starvation-induced autophagy requires Atg11, an adaptor protein for selective autophagy, but not its cargo recognition domain...
  16. Mao K, Liu X, Feng Y, Klionsky D. The progression of peroxisomal degradation through autophagy requires peroxisomal division. Autophagy. 2014;10:652-61 pubmed publisher
    ..When pexophagy is induced, both Dnm1 and Vps1 are recruited to the degrading peroxisomes through interactions with Atg11 and Atg36...
  17. Kanki T, Kurihara Y, Jin X, Goda T, Ono Y, Aihara M, et al. Casein kinase 2 is essential for mitophagy. EMBO Rep. 2013;14:788-94 pubmed publisher
    ..in yeast, the mitochondrial outer membrane protein Atg32 is phosphorylated, interacts with the adaptor protein Atg11 and is recruited into the vacuole with mitochondria...
  18. Mao K, Chew L, Inoue Aono Y, Cheong H, Nair U, Popelka H, et al. Atg29 phosphorylation regulates coordination of the Atg17-Atg31-Atg29 complex with the Atg11 scaffold during autophagy initiation. Proc Natl Acad Sci U S A. 2013;110:E2875-84 pubmed publisher
    ..its function; alanine substitution at the phosphorylation sites blocks its interaction with the scaffold protein Atg11 and its ability to facilitate assembly of the PAS...
  19. Lipatova Z, Segev N. A Role for Macro-ER-Phagy in ER Quality Control. PLoS Genet. 2015;11:e1005390 pubmed publisher
  20. Mochida K, Oikawa Y, Kimura Y, Kirisako H, Hirano H, Ohsumi Y, et al. Receptor-mediated selective autophagy degrades the endoplasmic reticulum and the nucleus. Nature. 2015;522:359-62 pubmed publisher
    ..Our results provide fundamental insight into the pathophysiological roles and mechanisms of 'ER-phagy' and 'nucleophagy' in other organisms. ..
  21. Lipatova Z, Shah A, Kim J, Mulholland J, Segev N. Regulation of ER-phagy by a Ypt/Rab GTPase module. Mol Biol Cell. 2013;24:3133-44 pubmed publisher
    ..We therefore propose that individual Ypt/Rabs, in the context of distinct modules, can coordinate alternative trafficking steps from one cellular compartment to different destinations...
  22. He C, Baba M, Cao Y, Klionsky D. Self-interaction is critical for Atg9 transport and function at the phagophore assembly site during autophagy. Mol Biol Cell. 2008;19:5506-16 pubmed publisher
    ..Our results support a model in which Atg9 multimerization facilitates membrane flow to the PAS for phagophore formation. ..
  23. Kamber R, Shoemaker C, Denic V. Receptor-Bound Targets of Selective Autophagy Use a Scaffold Protein to Activate the Atg1 Kinase. Mol Cell. 2015;59:372-81 pubmed publisher
    ..multisubunit complex comprising constitutive protein aggregates, their autophagy receptor, and a scaffold protein, Atg11. Development of a cell-free assay for Atg1-mediated phosphorylation enabled us to activate Atg1 with purified ..
  24. Liu X, Mao K, Yu A, Omairi Nasser A, Austin J, Glick B, et al. The Atg17-Atg31-Atg29 Complex Coordinates with Atg11 to Recruit the Vam7 SNARE and Mediate Autophagosome-Vacuole Fusion. Curr Biol. 2016;26:150-160 pubmed publisher
    ..Here, we identified a novel role of this complex in a late stage of autophagy where it coordinates with Atg11 to regulate autophagy-specific fusion with the vacuole...
  25. Wang K, Jin M, Liu X, Klionsky D. Proteolytic processing of Atg32 by the mitochondrial i-AAA protease Yme1 regulates mitophagy. Autophagy. 2013;9:1828-36 pubmed publisher
    ..selectivity for mitochondria sequestration as a cargo by the autophagic machinery through its interaction with Atg11, a scaffold protein for selective types of autophagy...
  26. Teixeira V, Medeiros T, Vilaça R, Pereira A, Chaves S, Côrte Real M, et al. Ceramide signalling impinges on Sit4p and Hog1p to promote mitochondrial fission and mitophagy in Isc1p-deficient cells. Cell Signal. 2015;27:1840-9 pubmed publisher
  27. Backues S, Orban D, Bernard A, Singh K, Cao Y, Klionsky D. Atg23 and Atg27 act at the early stages of Atg9 trafficking in S. cerevisiae. Traffic. 2015;16:172-90 pubmed publisher
    ..We used 'in vivo reconstitution' of this process in a multiple-knockout strain to define four proteins, Atg11, Atg19, Atg23 and Atg27, as the core minimal machinery necessary and sufficient for the trafficking of Atg9 to the ..
  28. He C, Song H, Yorimitsu T, Monastyrska I, Yen W, Legakis J, et al. Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast. J Cell Biol. 2006;175:925-35 pubmed
    ..in Atg9 cycling, we performed a yeast two-hybrid-based screen and identified a peripheral membrane protein, Atg11, that interacts with Atg9. We show that Atg11 governs Atg9 cycling through the PAS during specific autophagy...
  29. Pfaffenwimmer T, Reiter W, Brach T, Nogellova V, Papinski D, Schuschnig M, et al. Hrr25 kinase promotes selective autophagy by phosphorylating the cargo receptor Atg19. EMBO Rep. 2014;15:862-70 pubmed publisher
    ..Selective autophagy is mediated by cargo receptors, which link the cargo to the scaffold protein Atg11 and to Atg8 family proteins on the forming autophagosomal membrane...
  30. Motley A, Nuttall J, Hettema E. Pex3-anchored Atg36 tags peroxisomes for degradation in Saccharomyces cerevisiae. EMBO J. 2012;31:2852-68 pubmed publisher
    ..Furthermore, Atg36 binds Atg8 and the adaptor Atg11 that links receptors for selective types of autophagy to the core autophagy machinery...
  31. Chapa y Lazo B, Ayscough K. Apm4, the mu subunit of yeast AP-2 interacts with Pkc1, and mutation of the Pkc1 consensus phosphorylation site Thr176 inhibits AP-2 recruitment to endocytic sites. Commun Integr Biol. 2014;7:e28522 pubmed publisher
  32. Tanaka C, Tan L, Mochida K, Kirisako H, Koizumi M, Asai E, et al. Hrr25 triggers selective autophagy-related pathways by phosphorylating receptor proteins. J Cell Biol. 2014;207:91-105 pubmed publisher
    ..Hrr25-mediated phosphorylation enhances the interactions of these receptors with the common adaptor Atg11, which recruits the core autophagy-related proteins that mediate the formation of the autophagosomal membrane...
  33. Cao Y, Cheong H, Song H, Klionsky D. In vivo reconstitution of autophagy in Saccharomyces cerevisiae. J Cell Biol. 2008;182:703-13 pubmed publisher
    ..In vivo reconstitution enables the study of autophagy within the context of the complex regulatory networks that control this process, an analysis that is not possible with an in vitro system. ..
  34. Cao Y, Nair U, Yasumura Yorimitsu K, Klionsky D. A multiple ATG gene knockout strain for yeast two-hybrid analysis. Autophagy. 2009;5:699-705 pubmed
    ..We also probed a new interaction using the MKO Y2H strain, and our results suggest that Atg29 and Atg31 interact independently of other known Atg proteins, and this interaction may mediate the interaction between Atg17 and Atg29. ..