ASF1

Summary

Gene Symbol: ASF1
Description: nucleosome assembly factor ASF1
Alias: CIA1, nucleosome assembly factor ASF1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Lin L, Schultz M. Promoter regulation by distinct mechanisms of functional interplay between lysine acetylase Rtt109 and histone chaperone Asf1. Proc Natl Acad Sci U S A. 2011;108:19599-604 pubmed publisher
    ..This modification of H3 is performed by lysine acetylase Rtt109 acting in concert with histone chaperone Asf1. We have examined the contributions of Rtt109, Asf1, and H3 K56 acetylation to nutrient regulation of a well-..
  2. Morillo Huesca M, Maya D, Muñoz Centeno M, Singh R, Oreal V, Reddy G, et al. FACT prevents the accumulation of free histones evicted from transcribed chromatin and a subsequent cell cycle delay in G1. PLoS Genet. 2010;6:e1000964 pubmed publisher
  3. Tyler J, Adams C, Chen S, Kobayashi R, Kamakaka R, Kadonaga J. The RCAF complex mediates chromatin assembly during DNA replication and repair. Nature. 1999;402:555-60 pubmed
    ..RCAF comprises the Drosophila homologue of anti-silencing function 1 protein ASF1 and histones H3 and H4...
  4. English C, Maluf N, Tripet B, Churchill M, Tyler J. ASF1 binds to a heterodimer of histones H3 and H4: a two-step mechanism for the assembly of the H3-H4 heterotetramer on DNA. Biochemistry. 2005;44:13673-82 pubmed
    ..Antisilencing function 1 (ASF1) is a major histone H3-H4 chaperone that deposits histones H3 and H4 onto DNA...
  5. Zabaronick S, Tyler J. The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase. Mol Cell Biol. 2005;25:652-60 pubmed
    ..Deletion of ASF1 or CAF-1 components led to global transcriptional misregulation, both activation and repression, of genes scattered ..
  6. Donham D, Scorgie J, Churchill M. The activity of the histone chaperone yeast Asf1 in the assembly and disassembly of histone H3/H4-DNA complexes. Nucleic Acids Res. 2011;39:5449-58 pubmed publisher
    ..Anti-silencing function 1 (Asf1) is a chaperone of the H3/H4 dimer that functions in both of these processes...
  7. Blackwell J, Wilkinson S, Mosammaparast N, Pemberton L. Mutational analysis of H3 and H4 N termini reveals distinct roles in nuclear import. J Biol Chem. 2007;282:20142-50 pubmed
    ..Acetylation may be important for modulating the interaction with transport factors and may play a role in the release of histones from karyopherins in the nucleus. ..
  8. Han J, Zhou H, Li Z, Xu R, Zhang Z. Acetylation of lysine 56 of histone H3 catalyzed by RTT109 and regulated by ASF1 is required for replisome integrity. J Biol Chem. 2007;282:28587-96 pubmed
    ..histone acetyltransferase (HAT) complex, with Rtt109 being the catalytic subunit, and histone chaperone Asf1 is required for this modification. Cells lacking Rtt109 are susceptible to perturbations in DNA replication...
  9. Singh R, Kabbaj M, Paik J, Gunjan A. Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis. Nat Cell Biol. 2009;11:925-33 pubmed publisher
    ..Regulated histone proteolysis has major implications for the maintenance of epigenetic marks on chromatin, genomic stability and the packaging of sperm DNA. ..

More Information

Publications66

  1. Osada S, Sutton A, Muster N, Brown C, Yates J, Sternglanz R, et al. The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1. Genes Dev. 2001;15:3155-68 pubmed
    ..Furthermore, the SAS complex is found to interact with chromatin assembly factor Asf1p, and asf1 mutants show silencing defects similar to mutants in the SAS complex...
  2. Natsume R, Eitoku M, Akai Y, Sano N, Horikoshi M, Senda T. Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4. Nature. 2007;446:338-41 pubmed
    CIA (CCG1-interacting factor A)/ASF1, which is the most conserved histone chaperone among the eukaryotes, was genetically identified as a factor for an anti-silencing function (Asf1) by yeast genetic screening...
  3. Lewis L, Karthikeyan G, Cassiano J, Resnick M. Reduction of nucleosome assembly during new DNA synthesis impairs both major pathways of double-strand break repair. Nucleic Acids Res. 2005;33:4928-39 pubmed
    ..These results demonstrate that defective assembly of nucleosomes during new DNA synthesis compromises each of the known pathways of DSB repair and that the effects can be indirect consequences of changes in silenced chromatin structure. ..
  4. Krawitz D, Kama T, Kaufman P. Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing. Mol Cell Biol. 2002;22:614-25 pubmed
    ..We also observed a weak interaction between Asf1p and Cac2p in vitro, and we hypothesize that this interaction underlies the functional synergy between these histone deposition proteins. ..
  5. Franco A, Lam W, Burgers P, Kaufman P. Histone deposition protein Asf1 maintains DNA replisome integrity and interacts with replication factor C. Genes Dev. 2005;19:1365-75 pubmed
    ..Here we demonstrate that chromatin assembly factor Asf1 also affects DNA replication...
  6. Clemente Ruiz M, González Prieto R, Prado F. Histone H3K56 acetylation, CAF1, and Rtt106 coordinate nucleosome assembly and stability of advancing replication forks. PLoS Genet. 2011;7:e1002376 pubmed publisher
  7. Takahata S, Yu Y, Stillman D. FACT and Asf1 regulate nucleosome dynamics and coactivator binding at the HO promoter. Mol Cell. 2009;34:405-15 pubmed publisher
    ..chromatin disassembly at different regions of the promoter, with Swi/Snf, the FACT chromatin reorganizer, and the Asf1 histone chaperone each required for nucleosome eviction at distinct promoter regions...
  8. Rufiange A, Jacques P, Bhat W, Robert F, Nourani A. Genome-wide replication-independent histone H3 exchange occurs predominantly at promoters and implicates H3 K56 acetylation and Asf1. Mol Cell. 2007;27:393-405 pubmed
    ..Finally, we found that transcription-dependent incorporation of H3 at promoters is highly dependent on Asf1. Taken together, our data underline the dynamic nature of replication-independent nucleosome assembly/disassembly, ..
  9. Sutton A, Bucaria J, Osley M, Sternglanz R. Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription. Genetics. 2001;158:587-96 pubmed
    ..b>ASF1 is a highly conserved protein that has been implicated in transcriptional silencing and chromatin assembly...
  10. Gradolatto A, Rogers R, Lavender H, Taverna S, Allis C, Aitchison J, et al. Saccharomyces cerevisiae Yta7 regulates histone gene expression. Genetics. 2008;179:291-304 pubmed publisher
    ..Our work suggests a mechanism in which Yta7 is localized to chromatin to establish regions of transcriptional silencing, and that one facet of this cellular mechanism is to modulate transcription of histone genes. ..
  11. Ge Z, Wang H, Parthun M. Nuclear Hat1p complex (NuB4) components participate in DNA repair-linked chromatin reassembly. J Biol Chem. 2011;286:16790-9 pubmed publisher
    ..14 and 23 to arginine, has a pronounced effect on chromatin reassembly that is similar to that observed in an asf1?. The role of Hat1p and Hif1p is at least partially redundant with the role of Asf1p...
  12. Chimura T, Kuzuhara T, Horikoshi M. Identification and characterization of CIA/ASF1 as an interactor of bromodomains associated with TFIID. Proc Natl Acad Sci U S A. 2002;99:9334-9 pubmed
    ..Furthermore, yeast strain lacking the BDF1 gene shows the Spt phenotype that is shown also by the ASF1 gene disruptant, and a double-knockout strain of both genes shows synthetic lethality, indicating that ASF1 ..
  13. Sharp J, Fouts E, Krawitz D, Kaufman P. Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing. Curr Biol. 2001;11:463-73 pubmed
    ..However, the relationship between the contributions of HIR genes and ASF1 to silencing has not previously been explored...
  14. Campos E, Fillingham J, Li G, Zheng H, Voigt P, Kuo W, et al. The program for processing newly synthesized histones H3.1 and H4. Nat Struct Mol Biol. 2010;17:1343-51 pubmed publisher
    ..We further demonstrate the high degree of conservation for this pathway between higher and lower eukaryotes. ..
  15. Minard L, Lin L, Schultz M. SWI/SNF and Asf1 independently promote derepression of the DNA damage response genes under conditions of replication stress. PLoS ONE. 2011;6:e21633 pubmed publisher
    The histone chaperone Asf1 and the chromatin remodeler SWI/SNF have been separately implicated in derepression of the DNA damage response (DDR) genes in yeast cells treated with genotoxins that cause replication interference...
  16. Feser J, Truong D, Das C, Carson J, KIEFT J, Harkness T, et al. Elevated histone expression promotes life span extension. Mol Cell. 2010;39:724-35 pubmed publisher
    ..Indeed, yeast lacking the histone chaperone Asf1 or acetylation of histone H3 on lysine 56 are short lived, and this appears to be at least partly due to their ..
  17. Abshiru N, Ippersiel K, Tang Y, Yuan H, Marmorstein R, Verreault A, et al. Chaperone-mediated acetylation of histones by Rtt109 identified by quantitative proteomics. J Proteomics. 2013;81:80-90 pubmed publisher
    Rtt109 is a fungal-specific histone acetyltransferase (HAT) that associates with either Vps75 or Asf1 to acetylate histone H3...
  18. Kats E, Albuquerque C, Zhou H, Kolodner R. Checkpoint functions are required for normal S-phase progression in Saccharomyces cerevisiae RCAF- and CAF-I-defective mutants. Proc Natl Acad Sci U S A. 2006;103:3710-5 pubmed
    ..Here, we have investigated their role in checkpoints and S-phase progression. FACS analysis of mutants lacking Asf1 or Cac1 as well as various checkpoint proteins indicated that normal rates of S-phase progression in asf1 mutants ..
  19. Tang Y, Holbert M, Delgoshaie N, Wurtele H, Guillemette B, Meeth K, et al. Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation. Structure. 2011;19:221-31 pubmed publisher
    ..K9, K27, and K56 of histone H3 through interaction with either of two distinct histone chaperones, Vps75 or Asf1. We report the crystal structure of an Rtt109-AcCoA/Vps75 complex revealing an elongated Vps75 homodimer bound to ..
  20. Yang J, Freudenreich C. The Rtt109 histone acetyltransferase facilitates error-free replication to prevent CAG/CTG repeat contractions. DNA Repair (Amst). 2010;9:414-20 pubmed publisher
    ..These include Rtt109, a histone acetyltransferase, responsible for acetylating H3K56, Asf1, a histone H3/H4 chaperone, and Hst3 and Hst4, histone deacetylases which remove the acetyl group from H3K56...
  21. Tamburini B, Carson J, Linger J, Tyler J. Dominant mutants of the Saccharomyces cerevisiae ASF1 histone chaperone bypass the need for CAF-1 in transcriptional silencing by altering histone and Sir protein recruitment. Genetics. 2006;173:599-610 pubmed
    ..In this work we have identified mutations in the nonconserved C terminus of antisilencing function 1 (Asf1) that result in enhanced silencing of HMR and telomere-proximal reporters, overcoming the requirement for CAF-1 in ..
  22. Green E, Antczak A, Bailey A, Franco A, Wu K, Yates J, et al. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005;15:2044-9 pubmed
    ..The Hir proteins and CAF-1 share a common partner, the highly conserved histone H3/H4 binding protein Asf1, which binds the middle subunit of CAF-1 as well as to Hir proteins ...
  23. Scorgie J, Donham D, Churchill M. Analysis of histone chaperone antisilencing function 1 interactions. Methods Enzymol. 2012;512:223-41 pubmed publisher
    ..approaches for analysis of the interactions of a major chaperone of the H3/H4 dimer, anti-silencing function 1 (Asf1) with histones H3/H4, and DNA...
  24. Jiao Y, Seeger K, Lautrette A, Gaubert A, Mousson F, Guerois R, et al. Surprising complexity of the Asf1 histone chaperone-Rad53 kinase interaction. Proc Natl Acad Sci U S A. 2012;109:2866-71 pubmed publisher
    The histone chaperone Asf1 and the checkpoint kinase Rad53 are found in a complex in budding yeast cells in the absence of genotoxic stress. Our data suggest that this complex involves at least three interaction sites...
  25. Schwabish M, Struhl K. Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II. Mol Cell. 2006;22:415-22 pubmed
    ..Here, we show that the histone chaperone Asf1 associates with promoters and coding regions of transcriptionally active genes...
  26. Meijsing S, Ehrenhofer Murray A. The silencing complex SAS-I links histone acetylation to the assembly of repressed chromatin by CAF-I and Asf1 in Saccharomyces cerevisiae. Genes Dev. 2001;15:3169-82 pubmed
    ..the largest subunit of Saccharomyces cerevisiae chromatin assembly factor-I (CAF-I), and with the nucleosome assembly factor Asf1. The deletions of CAC1 (cac1Delta), ASF1 (asf1Delta), and SAS2 (sas2Delta) had similar effects on ..
  27. Minard L, Williams J, Walker A, Schultz M. Transcriptional regulation by Asf1: new mechanistic insights from studies of the DNA damage response to replication stress. J Biol Chem. 2011;286:7082-92 pubmed publisher
    b>Asf1 is a conserved histone H3/H4 chaperone. We find that Asf1 in budding yeast promotes an essential cellular response to replication stress caused by the ribonucleotide reductase inhibitor hydroxyurea...
  28. Kim H, Seol J, Han J, Youn H, Cho E. Histone chaperones regulate histone exchange during transcription. EMBO J. 2007;26:4467-74 pubmed
    ..This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, using a ..
  29. Kim J, Haber J. Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete. Proc Natl Acad Sci U S A. 2009;106:1151-6 pubmed publisher
    ..Here, we show the involvement of two chromatin assembly factors (CAFs), Asf1 and CAF-1, in turning off the DNA damage checkpoint in budding yeast...
  30. Wang A, Aristizabal M, Ryan C, Krogan N, Kobor M. Key functional regions in the histone variant H2A.Z C-terminal docking domain. Mol Cell Biol. 2011;31:3871-84 pubmed publisher
    ..Z deposition complex SWR1-C, the histone chaperone Chz1, and histone H2B. These data are consistent with a model in which retaining the variant in chromatin after its deposition by SWR1-C is a crucial determinant of its function. ..
  31. Reis C, Campbell J. Contribution of Trf4/5 and the nuclear exosome to genome stability through regulation of histone mRNA levels in Saccharomyces cerevisiae. Genetics. 2007;175:993-1010 pubmed
    ..These results identify TRF4, TRF5, and RRP6 as new players in the regulation of histone mRNA levels in yeast. To our knowledge, the histone transcripts are the first mRNAs that are upregulated in Trf mutants. ..
  32. Adkins M, Carson J, English C, Ramey C, Tyler J. The histone chaperone anti-silencing function 1 stimulates the acetylation of newly synthesized histone H3 in S-phase. J Biol Chem. 2007;282:1334-40 pubmed
    Anti-silencing function 1 (Asf1) is a highly conserved chaperone of histones H3/H4 that assembles or disassembles chromatin during transcription, replication, and repair...
  33. Emili A, Schieltz D, Yates J, Hartwell L. Dynamic interaction of DNA damage checkpoint protein Rad53 with chromatin assembly factor Asf1. Mol Cell. 2001;7:13-20 pubmed
    ..Here we report that Rad53 interacts in a dynamic functional manner with Asf1, a chromatin assembly factor recently shown to mediate deposition of acetylated histones H3 and H4 onto newly ..
  34. Keck K, Pemberton L. Interaction with the histone chaperone Vps75 promotes nuclear localization and HAT activity of Rtt109 in vivo. Traffic. 2011;12:826-39 pubmed publisher
    ..In summary, our data suggest that Vps75 influences chromatin structure by regulating histone modification and through its histone chaperone functions. ..
  35. Fillingham J, Kainth P, Lambert J, van Bakel H, Tsui K, Pena Castillo L, et al. Two-color cell array screen reveals interdependent roles for histone chaperones and a chromatin boundary regulator in histone gene repression. Mol Cell. 2009;35:340-51 pubmed publisher
    ..We discovered that the histone chaperone Rtt106 functions in a pathway with two other chaperones, Asf1 and the HIR complex, to create a repressive chromatin structure at core histone promoters...
  36. Tsubota T, Berndsen C, Erkmann J, Smith C, Yang L, Freitas M, et al. Histone H3-K56 acetylation is catalyzed by histone chaperone-dependent complexes. Mol Cell. 2007;25:703-12 pubmed
    ..homology to known acetyltransferases, forms an active complex with either of two histone binding proteins, Asf1 or Vps75...
  37. Burgess R, Zhou H, Han J, Zhang Z. A role for Gcn5 in replication-coupled nucleosome assembly. Mol Cell. 2010;37:469-80 pubmed publisher
    ..These results demonstrate that Gcn5 regulates RC nucleosome assembly, in part, by promoting H3 association with CAF-1 via H3 acetylation. ..
  38. Lin L, Minard L, Johnston G, Singer R, Schultz M. Asf1 can promote trimethylation of H3 K36 by Set2. Mol Cell Biol. 2010;30:1116-29 pubmed publisher
    b>Asf1 is a conserved histone H3/H4 chaperone that can assemble and disassemble nucleosomes and promote histone acetylation. Set2 is an H3 K36 methyltransferase...
  39. Agez M, Chen J, Guerois R, van Heijenoort C, Thuret J, Mann C, et al. Structure of the histone chaperone ASF1 bound to the histone H3 C-terminal helix and functional insights. Structure. 2007;15:191-9 pubmed
    b>Asf1 is a histone chaperone that favors histone H3/H4 assembly and disassembly...
  40. Poveda A, Sendra R. Site specificity of yeast histone acetyltransferase B complex in vivo. FEBS J. 2008;275:2122-36 pubmed publisher
    ..Finally, the exchange of Lys for Arg at position 12 of histone H4 did not interfere with histone H4 association with the complex, but prevented acetylation on Lys5 by the HAT-B enzyme, in vivo as well as in vitro. ..
  41. Prado F, Cortes Ledesma F, Aguilera A. The absence of the yeast chromatin assembly factor Asf1 increases genomic instability and sister chromatid exchange. EMBO Rep. 2004;5:497-502 pubmed
    Histone chaperone Asf1 participates in heterochromatin silencing, DNA repair and regulation of gene expression, and promotes the assembly of DNA into chromatin in vitro...
  42. Sharp J, Rizki G, Kaufman P. Regulation of histone deposition proteins Asf1/Hir1 by multiple DNA damage checkpoint kinases in Saccharomyces cerevisiae. Genetics. 2005;171:885-99 pubmed
    CAF-1, Hir proteins, and Asf1 are histone H3/H4 binding proteins important for chromatin-mediated transcriptional silencing...
  43. Korber P, Barbaric S, Luckenbach T, Schmid A, Schermer U, Blaschke D, et al. The histone chaperone Asf1 increases the rate of histone eviction at the yeast PHO5 and PHO8 promoters. J Biol Chem. 2006;281:5539-45 pubmed
    ..In this study we have shown that the histone chaperone Asf1 increases the rate of histone eviction at the PHO5 promoter...
  44. English C, Adkins M, Carson J, Churchill M, Tyler J. Structural basis for the histone chaperone activity of Asf1. Cell. 2006;127:495-508 pubmed
    Anti-silencing function 1 (Asf1) is a highly conserved chaperone of histones H3/H4 that assembles or disassembles chromatin during transcription, replication, and repair...
  45. Hu F, Alcasabas A, Elledge S. Asf1 links Rad53 to control of chromatin assembly. Genes Dev. 2001;15:1061-6 pubmed
    ..Here we report that overproduction of the chromatin assembly factor Asf1 can suppress the Ts phenotype of mrc1rad53 double mutants and the HU sensitivity of rad53 mutants...
  46. Gkikopoulos T, Havas K, Dewar H, Owen Hughes T. SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter. Mol Cell Biol. 2009;29:4057-66 pubmed publisher
    ..We also present evidence indicating that the histone chaperone Asf1 assists in this...
  47. Li Q, Zhou H, Wurtele H, Davies B, Horazdovsky B, Verreault A, et al. Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assembly. Cell. 2008;134:244-55 pubmed publisher
    ..These results reveal a mechanism by which H3K56Ac regulates replication-coupled nucleosome assembly mediated by CAF-1 and Rtt106. ..
  48. Linger J, Tyler J. The yeast histone chaperone chromatin assembly factor 1 protects against double-strand DNA-damaging agents. Genetics. 2005;171:1513-22 pubmed
    ..during double-strand repair is distinct from that of another histone H3-H4 chaperone, anti-silencing function 1 (ASF1)...
  49. Ramey C, Howar S, Adkins M, Linger J, Spicer J, Tyler J. Activation of the DNA damage checkpoint in yeast lacking the histone chaperone anti-silencing function 1. Mol Cell Biol. 2004;24:10313-27 pubmed
    ..onto newly synthesized DNA by the action of chromatin assembly factors, including anti-silencing function 1 (ASF1)...
  50. Recht J, Tsubota T, Tanny J, Diaz R, Berger J, Zhang X, et al. Histone chaperone Asf1 is required for histone H3 lysine 56 acetylation, a modification associated with S phase in mitosis and meiosis. Proc Natl Acad Sci U S A. 2006;103:6988-93 pubmed
    ..Furthermore, the H3 K56ac modification is not observed in the absence of the histone chaperone Asf1. asf1delta and H3 K56R mutants exhibit similar sensitivity to DNA damaging agents...
  51. Mousson F, Lautrette A, Thuret J, Agez M, Courbeyrette R, Amigues B, et al. Structural basis for the interaction of Asf1 with histone H3 and its functional implications. Proc Natl Acad Sci U S A. 2005;102:5975-80 pubmed
    b>Asf1 is a conserved histone chaperone implicated in nucleosome assembly, transcriptional silencing, and the cellular response to DNA damage...
  52. Myung K, Pennaneach V, Kats E, Kolodner R. Saccharomyces cerevisiae chromatin-assembly factors that act during DNA replication function in the maintenance of genome stability. Proc Natl Acad Sci U S A. 2003;100:6640-5 pubmed
    ..These results indicate that coupling of chromatin assembly to DNA replication and DNA repair is critical to maintaining genome stability. ..
  53. Williams S, Truong D, Tyler J. Acetylation in the globular core of histone H3 on lysine-56 promotes chromatin disassembly during transcriptional activation. Proc Natl Acad Sci U S A. 2008;105:9000-5 pubmed publisher
    ..Indeed, promoter chromatin disassembly is driven by Rtt109 and Asf1-dependent acetylation of H3 K56...
  54. Park Y, Sudhoff K, Andrews A, Stargell L, Luger K. Histone chaperone specificity in Rtt109 activation. Nat Struct Mol Biol. 2008;15:957-64 pubmed
    ..Nap1 and Vps75 interact with histones and Rtt109 with comparable affinities. However, only Vps75 stimulates Rtt109 enzymatic activity. Our data highlight the functional specificity of Vps75 in Rtt109 activation. ..
  55. Adkins M, Tyler J. The histone chaperone Asf1p mediates global chromatin disassembly in vivo. J Biol Chem. 2004;279:52069-74 pubmed
    ..By contrast, asf1 mutants globally over-assemble their genome into chromatin, as suggested by decreased accessibility of their ..
  56. Klopf E, Paskova L, Sole C, Mas G, Petryshyn A, Posas F, et al. Cooperation between the INO80 complex and histone chaperones determines adaptation of stress gene transcription in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 2009;29:4994-5007 pubmed publisher
    ..A mutant lacking the Asf1-associated H3K56 acetyltransferase Rtt109 or Asf1 itself also showed enhanced stress-induced transcript levels...
  57. Antczak A, Tsubota T, Kaufman P, Berger J. Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics. BMC Struct Biol. 2006;6:26 pubmed
    The histone H3/H4 chaperone Asf1 (anti-silencing function 1) is required for the establishment and maintenance of proper chromatin structure, as well as for genome stability in eukaryotes...