Gene Symbol: ASC1
Description: guanine nucleotide-binding protein subunit beta
Alias: ASU9, CPC2, NAD1, guanine nucleotide-binding protein subunit beta
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Zhang L, Schroeder S, Fong N, Bentley D. Altered nucleosome occupancy and histone H3K4 methylation in response to 'transcriptional stress'. EMBO J. 2005;24:2379-90 pubmed
    ..This response identifies a new role for H3K4 trimethylation at the 3' end of the gene, as a chromatin mark associated with impaired pol II transcription. ..
  2. Hoffmann B, Mösch H, Sattlegger E, Barthelmess I, Hinnebusch A, Braus G. The WD protein Cpc2p is required for repression of Gcn4 protein activity in yeast in the absence of amino-acid starvation. Mol Microbiol. 1999;31:807-22 pubmed
    The CPC2 gene of the budding yeast Saccharomyces cerevisiae encodes a G beta-like WD protein which is involved in regulating the activity of the general control activator Gcn4p...
  3. Inada T, Winstall E, Tarun S, Yates J, Schieltz D, Sachs A. One-step affinity purification of the yeast ribosome and its associated proteins and mRNAs. RNA. 2002;8:948-58 pubmed
  4. Gerbasi V, Weaver C, Hill S, Friedman D, Link A. Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression. Mol Cell Biol. 2004;24:8276-87 pubmed
    ..Our data suggest that one of Asc1p's or RACK1's functions is to repress gene expression. ..
  5. Coyle S, Gilbert W, Doudna J. Direct link between RACK1 function and localization at the ribosome in vivo. Mol Cell Biol. 2009;29:1626-34 pubmed publisher
    ..These results provide the first direct evidence that RACK1 functions from the ribosome, implying a physical link between the eukaryotic ribosome and cell signaling pathways in vivo. ..
  6. Rachfall N, Schmitt K, Bandau S, Smolinski N, Ehrenreich A, Valerius O, et al. RACK1/Asc1p, a ribosomal node in cellular signaling. Mol Cell Proteomics. 2013;12:87-105 pubmed publisher
    ..This study reveals the major cellular processes affected in a Saccharomyces cerevisiae ?asc1 deletion background via de novo proteome and transcriptome analysis, as well as subsequent phenotypical ..
  7. Kouba T, Rutkai E, Karásková M, Valášek L. The eIF3c/NIP1 PCI domain interacts with RNA and RACK1/ASC1 and promotes assembly of translation preinitiation complexes. Nucleic Acids Res. 2012;40:2683-99 pubmed publisher
    ..The extreme C-terminus directly interacts with blades 1-3 of the small ribosomal protein RACK1/ASC1, which is a part of the 40S head, and, consistently, deletion of the ASC1 coding region likewise affects eIF3 ..
  8. Zeller C, Parnell S, Dohlman H. The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast. J Biol Chem. 2007;282:25168-76 pubmed
    ..Here we show that the yeast ortholog of RACK1 (receptor for activated protein kinase C1) Asc1 functions as the Gbeta for Gpa2...
  9. Melamed D, Bar Ziv L, Truzman Y, Arava Y. Asc1 supports cell-wall integrity near bud sites by a Pkc1 independent mechanism. PLoS ONE. 2010;5:e11389 pubmed publisher
    The yeast ribosomal protein Asc1 is a WD-protein family member...

More Information


  1. Chiabudini M, Tais A, Zhang Y, Hayashi S, Wölfle T, Fitzke E, et al. Release factor eRF3 mediates premature translation termination on polylysine-stalled ribosomes in Saccharomyces cerevisiae. Mol Cell Biol. 2014;34:4062-76 pubmed publisher
    ..polylysine synthesis was abolished when ribosome stalling was prevented due to the absence of the ribosomal protein Asc1. In contrast, premature termination was enhanced, when the general rate of translation elongation was lowered...
  2. Schütz S, Fischer U, Altvater M, Nerurkar P, Peña C, Gerber M, et al. A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly. elife. 2014;3:e03473 pubmed publisher
    ..We anticipate similar carriers-termed here escortins-to securely connect the nuclear import machinery with pathways that deposit r-proteins onto developing pre-ribosomal particles. ..
  3. Yatime L, Hein K, Nilsson J, Nissen P. Structure of the RACK1 dimer from Saccharomyces cerevisiae. J Mol Biol. 2011;411:486-98 pubmed publisher
    ..We discuss the significance of the dimer structure for RACK1 function. ..
  4. Moabbi A, Agarwal N, El Kaderi B, Ansari A. Role for gene looping in intron-mediated enhancement of transcription. Proc Natl Acad Sci U S A. 2012;109:8505-10 pubmed publisher
    ..To address this issue, we inserted an intron in INO1, which is a nonintronic gene, and deleted the intron from ASC1, which contains a natural intron...
  5. Kouba T, Dányi I, Gunišová S, Munzarová V, Vlčková V, Cuchalová L, et al. Small ribosomal protein RPS0 stimulates translation initiation by mediating 40S-binding of eIF3 via its direct contact with the eIF3a/TIF32 subunit. PLoS ONE. 2012;7:e40464 pubmed publisher
    ..Together we conclude that the interaction between the flexible RPS0A-CTT and the residues 200-400 of the a/TIF32-NTD significantly stimulates attachment of eIF3 and its associated eIFs to small ribosomal subunits in vivo. ..
  6. Luz J, Georg R, Gomes C, Machado Santelli G, Oliveira C. Sdo1p, the yeast orthologue of Shwachman-Bodian-Diamond syndrome protein, binds RNA and interacts with nuclear rRNA-processing factors. Yeast. 2009;26:287-98 pubmed publisher
    ..Sdo1p binds RNA directly, suggesting that it may associate with the ribosomal subunits also through RNA interaction. ..
  7. Valerius O, Kleinschmidt M, Rachfall N, Schulze F, López Marín S, Hoppert M, et al. The Saccharomyces homolog of mammalian RACK1, Cpc2/Asc1p, is required for FLO11-dependent adhesive growth and dimorphism. Mol Cell Proteomics. 2007;6:1968-79 pubmed
    ..Deletion of CPC2/ASC1 abolished amino acid starvation-induced adhesive growth and impaired basal expression of FLO11 and its ..
  8. Kleinschmidt M, Schulz R, Braus G. The yeast CPC2/ASC1 gene is regulated by the transcription factors Fhl1p and Ifh1p. Curr Genet. 2006;49:218-28 pubmed
    CPC2/ASC1 is one of the most abundantly transcribed genes in Saccharomyces cerevisiae. It encodes a ribosome-associated Gbeta-like WD protein, which is highly conserved from yeast to man...
  9. Tarnowski K, Fituch K, Szczepanowski R, Dadlez M, Kaus Drobek M. Patterns of structural dynamics in RACK1 protein retained throughout evolution: a hydrogen-deuterium exchange study of three orthologs. Protein Sci. 2014;23:639-51 pubmed publisher
    ..This was exemplified by our additional analysis of yeast RACK1 dimer, which showed stabilization, as well as destabilization, of several interface regions upon dimer formation. ..
  10. Peña C, Schütz S, Fischer U, Chang Y, Panse V. Prefabrication of a ribosomal protein subcomplex essential for eukaryotic ribosome formation. elife. 2016;5: pubmed publisher
    ..Thus, prefabrication of a native-like r-protein subcomplex drives efficient and accurate construction of the eukaryotic ribosome. ..
  11. Lee R, Brunette S, Puente L, Megeney L. Metacaspase Yca1 is required for clearance of insoluble protein aggregates. Proc Natl Acad Sci U S A. 2010;107:13348-53 pubmed publisher
    ..Together, our results show that Yca1 contributes to the fitness and adaptability of growing yeast through an aggregate remodeling activity. ..
  12. Chantrel Y, Gaisne M, Lions C, Verdiére J. The transcriptional regulator Hap1p (Cyp1p) is essential for anaerobic or heme-deficient growth of Saccharomyces cerevisiae: Genetic and molecular characterization of an extragenic suppressor that encodes a WD repeat protein. Genetics. 1998;148:559-69 pubmed
    ..extragenic suppressors of a hap1-hem1- strain allowed the identification, cloning, and molecular analysis of ASC1 (Cyp1 Absence of growth Supressor)...
  13. Phan V, Ding V, Li F, Chalkley R, Burlingame A, McCormick F. The RasGAP proteins Ira2 and neurofibromin are negatively regulated by Gpb1 in yeast and ETEA in humans. Mol Cell Biol. 2010;30:2264-79 pubmed publisher
    ..These findings provide evidence for conserved ubiquitination pathways regulating the RasGAP proteins Ira2 (in yeast) and neurofibromin (in humans). ..
  14. Galvão F, Rossi D, Silveira W, Valentini S, Zanelli C. The deoxyhypusine synthase mutant dys1-1 reveals the association of eIF5A and Asc1 with cell wall integrity. PLoS ONE. 2013;8:e60140 pubmed publisher
    ..We observed that the dys1-1 genetically interacts with the sole yeast protein kinase C (Pkc1) and Asc1, a component of the 40S ribosomal subunit...
  15. Sezen B, Seedorf M, Schiebel E. The SESA network links duplication of the yeast centrosome with the protein translation machinery. Genes Dev. 2009;23:1559-70 pubmed publisher
    ..comprising the GYF domain protein Smy2, the translation inhibitor Eap1, the mRNA-binding protein Scp160 and the Asc1 protein, specifically inhibits initiation of translation of POM34 mRNA that encodes an integral membrane protein of ..
  16. Basmaji F, Martin Yken H, Durand F, Dagkessamanskaia A, Pichereaux C, Rossignol M, et al. The 'interactome' of the Knr4/Smi1, a protein implicated in coordinating cell wall synthesis with bud emergence in Saccharomyces cerevisiae. Mol Genet Genomics. 2006;275:217-30 pubmed
    ..The last group contained four proteins (Asc1, Ubc1, Hsc82 and Gvp36) that probably deal with the stability/degradation of proteins...
  17. Wolf A, Grayhack E. Asc1, homolog of human RACK1, prevents frameshifting in yeast by ribosomes stalled at CGA codon repeats. RNA. 2015;21:935-45 pubmed publisher
    ..The conserved eukaryotic Asc1 protein limits translation through internal Arg CGA codon repeats...
  18. Schmitt K, Smolinski N, Neumann P, Schmaul S, Hofer Pretz V, Braus G, et al. Asc1p/RACK1 Connects Ribosomes to Eukaryotic Phosphosignaling. Mol Cell Biol. 2017;37: pubmed publisher
    ..of phosphosite T143 to a dephosphorylation-mimicking alanine, probably through proteasomal degradation, leading to asc1- phenotypes...
  19. Li J, Lu N, Huang B. [Roles of G-protein beta and gamma subunits in the interaction of G beta gamma with adenylyl cyclases II]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001;23:115-8 pubmed
  20. Sitron C, Park J, Brandman O. Asc1, Hel2, and Slh1 couple translation arrest to nascent chain degradation. RNA. 2017;23:798-810 pubmed publisher
    ..on two poorly understood 80S ribosome-binding proteins previously implicated in the response to failed translation, Asc1 and Hel2, as well as a new component of the pathway, Slh1, that we identified here...
  21. Limoncelli K, Merrikh C, Moore M. ASC1 and RPS3: new actors in 18S nonfunctional rRNA decay. RNA. 2017;23:1946-1960 pubmed publisher
    ..Complete stabilization of mutant 18S rRNA in dom34Δ;asc1Δ and hbs1Δ;asc1Δ strains indicates the existence of two genetically separable 18S NRD ..