ARP2

Summary

Gene Symbol: ARP2
Description: actin-related protein 2
Alias: ACT2, actin-related protein 2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Winter D, Choe E, Li R. Genetic dissection of the budding yeast Arp2/3 complex: a comparison of the in vivo and structural roles of individual subunits. Proc Natl Acad Sci U S A. 1999;96:7288-93 pubmed
    In previous work, we identified the yeast Arp2/3 complex, which localizes to cortical actin patches and is required for their motility and integrity in vivo...
  2. Winter D, Podtelejnikov A, Mann M, Li R. The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches. Curr Biol. 1997;7:519-29 pubmed
    ..and biochemical experiments in vitro have implicated a multi-protein complex containing two actin-related proteins, Arp2 and Arp3, as a potential actin-filament nucleation factor...
  3. Pan F, Egile C, Lipkin T, Li R. ARPC1/Arc40 mediates the interaction of the actin-related protein 2 and 3 complex with Wiskott-Aldrich syndrome protein family activators. J Biol Chem. 2004;279:54629-36 pubmed
    The actin-related protein 2 and 3 (Arp2/3) complex is a seven-subunit protein complex that nucleates actin filaments at the cell cortex...
  4. Moreau V, Galan J, Devilliers G, Haguenauer Tsapis R, Winsor B. The yeast actin-related protein Arp2p is required for the internalization step of endocytosis. Mol Biol Cell. 1997;8:1361-75 pubmed
    ..We have tested its potential role in the endocytic and exocytic pathways by using a temperature-sensitive allele, arp2-1...
  5. Madania A, Dumoulin P, Grava S, Kitamoto H, Schärer Brodbeck C, Soulard A, et al. The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex. Mol Biol Cell. 1999;10:3521-38 pubmed
    ..Here we show that Las17p interacts with the Arp2/3 complex...
  6. Smith B, Daugherty Clarke K, Goode B, Gelles J. Pathway of actin filament branch formation by Arp2/3 complex revealed by single-molecule imaging. Proc Natl Acad Sci U S A. 2013;110:1285-90 pubmed publisher
    ..motility and endocytosis, yet key aspects of its mechanism are unknown due to a lack of real-time observations of Arp2/3 complex through the nucleation process...
  7. Volkmann N, Amann K, Stoilova McPhie S, Egile C, Winter D, Hazelwood L, et al. Structure of Arp2/3 complex in its activated state and in actin filament branch junctions. Science. 2001;293:2456-9 pubmed
    The seven-subunit Arp2/3 complex choreographs the formation of branched actin networks at the leading edge of migrating cells...
  8. Kaminska J, Gajewska B, Hopper A, Zoładek T. Rsp5p, a new link between the actin cytoskeleton and endocytosis in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:6946-8 pubmed
    ..rsp5 arp2, rsp5 end3, and rsp5 sla2 double mutants all show synthetic growth defects...
  9. Kadota J, Yamamoto T, Yoshiuchi S, Bi E, Tanaka K. Septin ring assembly requires concerted action of polarisome components, a PAK kinase Cla4p, and the actin cytoskeleton in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:5329-45 pubmed
    ..Our results suggest that polarisome components and Cla4p are required for the initial assembly of the septin ring and that the actin cytoskeleton is involved in this process. ..

More Information

Publications50

  1. Toi H, Fujimura Kamada K, Irie K, Takai Y, Todo S, Tanaka K. She4p/Dim1p interacts with the motor domain of unconventional myosins in the budding yeast, Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:2237-49 pubmed
    ..Our results suggest that She4p/Dim1p is required for structural integrity or regulation of the motor domain of unconventional myosins. ..
  2. Boldogh I, Yang H, Nowakowski W, Karmon S, Hays L, Yates J, et al. Arp2/3 complex and actin dynamics are required for actin-based mitochondrial motility in yeast. Proc Natl Acad Sci U S A. 2001;98:3162-7 pubmed
    The Arp2/3 complex is implicated in actin polymerization-driven movement of Listeria monocytogenes...
  3. Lechler T, Shevchenko A, Li R. Direct involvement of yeast type I myosins in Cdc42-dependent actin polymerization. J Cell Biol. 2000;148:363-73 pubmed
    ..The type I myosins also interact with the Arp2/3 complex through a sequence at the end of the tail domain homologous to the Arp2/3-activating region of WASP-like ..
  4. Winter D, Lechler T, Li R. Activation of the yeast Arp2/3 complex by Bee1p, a WASP-family protein. Curr Biol. 1999;9:501-4 pubmed
    The Arp2/3 complex is a highly conserved cytoskeletal component that has been implicated in the nucleation of actin filament assembly...
  5. Moreau V, Madania A, Martin R, Winson B. The Saccharomyces cerevisiae actin-related protein Arp2 is involved in the actin cytoskeleton. J Cell Biol. 1996;134:117-32 pubmed
    Arp2p is an essential yeast actin-related protein. Disruption of the corresponding ARP2 gene leads to a terminal phenotype characterized by the presence of a single large bud...
  6. Duncan M, Cope M, Goode B, Wendland B, Drubin D. Yeast Eps15-like endocytic protein, Pan1p, activates the Arp2/3 complex. Nat Cell Biol. 2001;3:687-90 pubmed
    ..The yeast Arp2/3 complex is required for the internalization step of endocytosis...
  7. Goode B, Rodal A, Barnes G, Drubin D. Activation of the Arp2/3 complex by the actin filament binding protein Abp1p. J Cell Biol. 2001;153:627-34 pubmed
    ..diverse processes, many proteins are likely to make spatially and temporally regulated interactions with the Arp2/3 complex. We have isolated a new activator, Abp1p, which associates tightly with the yeast Arp2/3 complex...
  8. Fehrenbacher K, Boldogh I, Pon L. A role for Jsn1p in recruiting the Arp2/3 complex to mitochondria in budding yeast. Mol Biol Cell. 2005;16:5094-102 pubmed
    Although the Arp2/3 complex localizes to the leading edge of motile cells, endocytic structures, and mitochondria in budding yeast, the mechanism for targeting the Arp2/3 complex to different regions in the cell is not well understood...
  9. Gilbert C, Kristjuhan A, Winkler G, Svejstrup J. Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation. Mol Cell. 2004;14:457-64 pubmed
    ..These results strongly support a role for Elongator in transcript elongation. ..
  10. Martin A, Xu X, Rouiller I, Kaksonen M, Sun Y, Belmont L, et al. Effects of Arp2 and Arp3 nucleotide-binding pocket mutations on Arp2/3 complex function. J Cell Biol. 2005;168:315-28 pubmed
    Contributions of actin-related proteins (Arp) 2 and 3 nucleotide state to Arp2/3 complex function were tested using nucleotide-binding pocket (NBP) mutants in Saccharomyces cerevisiae...
  11. Daugherty K, Goode B. Functional surfaces on the p35/ARPC2 subunit of Arp2/3 complex required for cell growth, actin nucleation, and endocytosis. J Biol Chem. 2008;283:16950-9 pubmed publisher
    The Arp2/3 complex is comprised of seven evolutionarily conserved subunits and upon activation by WASp or another nucleation promoting factor nucleates the formation of actin filaments...
  12. Ganusova E, Ozolins L, Bhagat S, Newnam G, Wegrzyn R, Sherman M, et al. Modulation of prion formation, aggregation, and toxicity by the actin cytoskeleton in yeast. Mol Cell Biol. 2006;26:617-29 pubmed
    ..These aggregates promote prion formation. Moreover, it appears that the actin cytoskeleton also plays a certain role in counteracting the toxicity of the overproduced potentially aggregating proteins. ..
  13. Tyler J, Allwood E, Ayscough K. WASP family proteins, more than Arp2/3 activators. Biochem Soc Trans. 2016;44:1339-1345 pubmed
    ..characterized as factors that promote the nucleation of actin through the activation of the protein complex Arp2/3...
  14. Doolittle L, Rosen M, Padrick S. Purification of Arp2/3 complex from Saccharomyces cerevisiae. Methods Mol Biol. 2013;1046:251-71 pubmed publisher
    ..The Arp2/3 complex is a principal actin nucleation factor used throughout the eukaryotic family tree...
  15. Jarmoszewicz K, Łukasiak K, Riezman H, Kaminska J. Rsp5 ubiquitin ligase is required for protein trafficking in Saccharomyces cerevisiae COPI mutants. PLoS ONE. 2012;7:e39582 pubmed publisher
    ..Additionally, Rsp5 and Sla1 proteins were found by co-immunoprecipitation in a complex containing COPI subunits. Together, our results show that Rsp5 ligase plays a role in regulating retrograde Golgi-to-ER trafficking. ..
  16. Liu S, Needham K, May J, Nolen B. Mechanism of a concentration-dependent switch between activation and inhibition of Arp2/3 complex by coronin. J Biol Chem. 2011;286:17039-46 pubmed publisher
    b>Arp2/3 complex is a key actin filament nucleator that assembles branched actin networks in response to cellular signals. The activity of Arp2/3 complex is regulated by both activating and inhibitory proteins...
  17. Rodal A, Sokolova O, Robins D, Daugherty K, Hippenmeyer S, Riezman H, et al. Conformational changes in the Arp2/3 complex leading to actin nucleation. Nat Struct Mol Biol. 2005;12:26-31 pubmed
    The two actin-related subunits of the Arp2/3 complex, Arp2 and Arp3, are proposed to form a pseudo actin dimer that nucleates actin polymerization...
  18. Yan C, Leibowitz N, Melese T. A role for the divergent actin gene, ACT2, in nuclear pore structure and function. EMBO J. 1997;16:3572-86 pubmed
    We have identified a temperature-sensitive allele of the yeast divergent actin gene ACT2, act2-1, which displays defects in nuclear pore complex (NPC) structure and nuclear import at the restrictive temperature...
  19. Toshima J, Toshima J, Martin A, Drubin D. Phosphoregulation of Arp2/3-dependent actin assembly during receptor-mediated endocytosis. Nat Cell Biol. 2005;7:246-54 pubmed
    ..Pan1p is an Arp2/3 activator and here we show that this activity is dependent on F-actin binding...
  20. Boettner D, D Agostino J, Torres O, Daugherty Clarke K, Uygur A, Reider A, et al. The F-BAR protein Syp1 negatively regulates WASp-Arp2/3 complex activity during endocytic patch formation. Curr Biol. 2009;19:1979-87 pubmed publisher
    Actin polymerization by Arp2/3 complex must be tightly regulated to promote clathrin-mediated endocytosis. Although many Arp2/3 complex activators have been identified, mechanisms for its negative regulation have remained more elusive...
  21. Colomina N, Ferrezuelo F, Vergés E, Aldea M, Garí E. Whi3 regulates morphogenesis in budding yeast by enhancing Cdk functions in apical growth. Cell Cycle. 2009;8:1912-20 pubmed
    ..We propose that Whi3 is required for the apical localization of Cdc28-Cln1,2 complexes during bud growth and thereby, to promote the activation of Cdc42 and its effectors in the bud apex. ..
  22. Tomala K, Korona R. Alleviation of deleterious effects of protein mutation through inactivation of molecular chaperones. Mol Genet Genomics. 2008;280:409-17 pubmed publisher
    ..We suggest that under non-stressful conditions, molecular chaperones are tuned to support folding of native proteins, but not that of mutated ones. ..
  23. Martin A, Welch M, Drubin D. Arp2/3 ATP hydrolysis-catalysed branch dissociation is critical for endocytic force generation. Nat Cell Biol. 2006;8:826-33 pubmed
    The Arp2/3 complex, which is crucial for actin-based motility, nucleates actin filaments and organizes them into y-branched networks...
  24. Wen K, Rubenstein P. Acceleration of yeast actin polymerization by yeast Arp2/3 complex does not require an Arp2/3-activating protein. J Biol Chem. 2005;280:24168-74 pubmed
    The Arp2/3 complex creates filament branches leading to an enhancement in the rate of actin polymerization...
  25. Jin M, Cai M. A novel function of Arp2p in mediating Prk1p-specific regulation of actin and endocytosis in yeast. Mol Biol Cell. 2008;19:297-307 pubmed
    ..its dual function in endocytic complex assembly and activation of the actin polymerization initiation complex Arp2/3p...
  26. Taxis C, Maeder C, Reber S, Rathfelder N, Miura K, Greger K, et al. Dynamic organization of the actin cytoskeleton during meiosis and spore formation in budding yeast. Traffic. 2006;7:1628-42 pubmed
    ..However, actin is essential for spore wall formation. This requires the function of the Arp2/3p complex and involves large carbohydrate-rich compartments, which may be chitosome analogous structures.
  27. Monastyrska I, He C, Geng J, Hoppe A, Li Z, Klionsky D. Arp2 links autophagic machinery with the actin cytoskeleton. Mol Biol Cell. 2008;19:1962-75 pubmed publisher
    ..Our results reveal that an actin-related protein, Arp2, briefly colocalizes with Atg9 and directly regulates the dynamics of Atg9 movement...
  28. Rodnick Smith M, Luan Q, Liu S, Nolen B. Role and structural mechanism of WASP-triggered conformational changes in branched actin filament nucleation by Arp2/3 complex. Proc Natl Acad Sci U S A. 2016;113:E3834-43 pubmed publisher
    The Arp2/3 (Actin-related proteins 2/3) complex is activated by WASP (Wiskott-Aldrich syndrome protein) family proteins to nucleate branched actin filaments that are important for cellular motility...
  29. Yamamoto T, Mochida J, Kadota J, Takeda M, Bi E, Tanaka K. Initial polarized bud growth by endocytic recycling in the absence of actin cable-dependent vesicle transport in yeast. Mol Biol Cell. 2010;21:1237-52 pubmed publisher
    ..Additional mutations in components for cortical actin patches, which are assembled by the Arp2/3 complex to play a pivotal role in endocytic vesicle formation, inhibited this budding...
  30. Carabetta V, Li T, Shakya A, Greco T, Cristea I. Integrating Lys-N proteolysis and N-terminal guanidination for improved fragmentation and relative quantification of singly-charged ions. J Am Soc Mass Spectrom. 2010;21:1050-60 pubmed publisher
    ..investigated the suitability of Lys-N proteolysis for use with MALDI mass spectrometry to characterize the yeast Arp2 complex and E. coli PAP I protein interactions...
  31. Liu S, May J, Helgeson L, Nolen B. Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex. J Biol Chem. 2013;288:487-97 pubmed publisher
    The Arp2/3 (actin-related protein 2/3) complex nucleates branched actin filaments involved in multiple cellular functions, including endocytosis and cellular motility...
  32. Humphries C, Balcer H, D Agostino J, Winsor B, Drubin D, Barnes G, et al. Direct regulation of Arp2/3 complex activity and function by the actin binding protein coronin. J Cell Biol. 2002;159:993-1004 pubmed
    Mechanisms for activating the actin-related protein 2/3 (Arp2/3) complex have been the focus of many recent studies...
  33. Idrissi F, Wolf B, Geli M. Cofilin, but not profilin, is required for myosin-I-induced actin polymerization and the endocytic uptake in yeast. Mol Biol Cell. 2002;13:4074-87 pubmed
    ..Robust evidence also indicates that these proteins induce Arp2/3-dependent actin polymerization...
  34. Ydenberg C, Padrick S, Sweeney M, Gandhi M, Sokolova O, Goode B. GMF severs actin-Arp2/3 complex branch junctions by a cofilin-like mechanism. Curr Biol. 2013;23:1037-45 pubmed publisher
    ..filament networks driving cell motility, endocytosis, and intracellular transport are assembled in seconds by the Arp2/3 complex and must be equally rapidly debranched and turned over...
  35. Rajmohan R, Wong M, Meng L, Munn A, Thanabalu T. Las17p-Vrp1p but not Las17p-Arp2/3 interaction is important for actin patch polarization in yeast. Biochim Biophys Acta. 2009;1793:825-35 pubmed publisher
    ..Both yeast and human WASP bind to and stimulate the Arp2/3 complex which in turn nucleates assembly of actin monomers into filaments at polarized sites at the cortex...
  36. García Rodríguez L, Gay A, Pon L. Puf3p, a Pumilio family RNA binding protein, localizes to mitochondria and regulates mitochondrial biogenesis and motility in budding yeast. J Cell Biol. 2007;176:197-207 pubmed
    ..In addition, Puf3p links the mitochore, a complex required for mitochondrial-cytoskeletal interactions, to the Arp2/3 complex, the force generator for actin-dependent, bud-directed mitochondrial movement...
  37. Galletta B, Carlsson A, Cooper J. Molecular analysis of Arp2/3 complex activation in cells. Biophys J. 2012;103:2145-56 pubmed publisher
    ..In the dendritic nucleation model, Arp2/3 complex is critical, binding to the side of an existing mother filament, nucleating a new daughter filament, and ..
  38. Balcer H, Daugherty Clarke K, Goode B. The p40/ARPC1 subunit of Arp2/3 complex performs multiple essential roles in WASp-regulated actin nucleation. J Biol Chem. 2010;285:8481-91 pubmed publisher
    The Arp2/3 complex is a conserved seven-subunit actin-nucleating machine activated by WASp (Wiskott Aldrich syndrome protein)...
  39. Qureshi M, Sheikh Q, Hill R, Brown P, Dickman M, Tzokov S, et al. Affinity filtration coupled with capillary-based affinity purification for the isolation of protein complexes. Anal Biochem. 2013;439:47-9 pubmed publisher
    ..The application of the method is demonstrated for the yeast Arp2/3 heptameric protein complex involved in mediating reorganization of the actin cytoskeleton.
  40. Egile C, Rouiller I, Xu X, Volkmann N, Li R, Hanein D. Mechanism of filament nucleation and branch stability revealed by the structure of the Arp2/3 complex at actin branch junctions. PLoS Biol. 2005;3:e383 pubmed
    ..Assembly of actin branch junctions requires the Arp2/3 complex, upon activation, to initiate a new actin (daughter) filament branch from the side of an existing (mother)..
  41. D Agostino J, Goode B. Dissection of Arp2/3 complex actin nucleation mechanism and distinct roles for its nucleation-promoting factors in Saccharomyces cerevisiae. Genetics. 2005;171:35-47 pubmed
    Actin nucleation by the Arp2/3 complex is under tight control, remaining inactive until stimulation by nucleation-promoting factors (NPFs)...