Gene Symbol: ARP1
Description: actin-related protein 1
Alias: ACT5, actin-related protein 1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Muhua L, Karpova T, Cooper J. A yeast actin-related protein homologous to that in vertebrate dynactin complex is important for spindle orientation and nuclear migration. Cell. 1994;78:669-79 pubmed
    ..We have identified in S. cerevisiae a novel actin-related gene, ACT5, in the Arp1 family...
  2. Miller R, Rose M. Kar9p is a novel cortical protein required for cytoplasmic microtubule orientation in yeast. J Cell Biol. 1998;140:377-90 pubmed
    ..The kar9 mitotic defect was similar to mutations in dhc1/dyn1 (dynein heavy chain gene), jnm1, and act5. kar9Delta dhc1Delta, kar9Delta jnm1Delta, and kar9Delta act5Delta double mutants were synthetically lethal, ..
  3. Fujiwara T, Tanaka K, Inoue E, Kikyo M, Takai Y. Bni1p regulates microtubule-dependent nuclear migration through the actin cytoskeleton in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:8016-27 pubmed
    ..The arp1 bni1 mutant showed a synthetic lethal phenotype while the cin8 bni1 mutant did not, suggesting that Bni1p functions ..
  4. Woodruff J, Drubin D, Barnes G. Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment. Mol Biol Cell. 2009;20:3003-11 pubmed publisher
    ..We propose that She1p restricts dynein-dependent spindle positioning to anaphase by inhibiting the association of dynein with the complete dynactin complex. ..
  5. Kahana J, Schlenstedt G, Evanchuk D, Geiser J, Hoyt M, Silver P. The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B. Mol Biol Cell. 1998;9:1741-56 pubmed
    ..Taken together, these results suggest that the yeast dynactin complex and cytoplasmic dynein together define a physiological pathway that is responsible for spindle translocation late in anaphase. ..
  6. Maekawa H, Schiebel E. Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex. Genes Dev. 2004;18:1709-24 pubmed
    ..This transition prevents the preanaphase spindle from becoming prematurely pulled into the bud. Thus, Cdc28-Clb4 facilitates spindle alignment by regulating the interaction of astral microtubules with subdomains of the bud cortex. ..
  7. Clark S, Rose M. Arp10p is a pointed-end-associated component of yeast dynactin. Mol Biol Cell. 2006;17:738-48 pubmed
    ..Based on the hRAS-Arp1p assay, loss of Arp10p enhances the apparent association of dynactin with the plasma membrane and suppresses the loss of signaling conferred by cell wall damage. ..
  8. Cuschieri L, Miller R, Vogel J. Gamma-tubulin is required for proper recruitment and assembly of Kar9-Bim1 complexes in budding yeast. Mol Biol Cell. 2006;17:4420-34 pubmed
    ..We propose that SPBs, via the tail of Tub4, promote the assembly of functional +TIP complexes before their deployment to microtubule +ends. ..
  9. Clark S, Rose M. Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued. Mol Biol Cell. 2005;16:3999-4012 pubmed
    ..Unlike the actin mutations, none of the ARP1 alleles disrupt filament formation; however, one pointed-end allele delayed the elution of Arp1p on gel filtration, ..

More Information


  1. Bergman Z, Xia X, Amaro I, Huffaker T. Constitutive dynein activity in She1 mutants reveals differences in microtubule attachment at the yeast spindle pole body. Mol Biol Cell. 2012;23:2319-26 pubmed publisher
    ..Overall, these results indicate that inhibition of dynein activity by She1 is important to prevent excessive detachment of cytoplasmic microtubules, particularly in G1 cells. ..
  2. Liu W, Santiago Tirado F, Bretscher A. Yeast formin Bni1p has multiple localization regions that function in polarized growth and spindle orientation. Mol Biol Cell. 2012;23:412-22 pubmed publisher
    ..These results define an unexpected complexity in the mechanism of formin localization and function. ..
  3. Wu W, Alami S, Luk E, Wu C, Sen S, Mizuguchi G, et al. Swc2 is a widely conserved H2AZ-binding module essential for ATP-dependent histone exchange. Nat Struct Mol Biol. 2005;12:1064-71 pubmed
    ..Finally, the C-terminal alpha-helix of H2AZ is crucial for its recognition by SWR1. These findings provide insight on the initial events of histone exchange. ..
  4. Moore J, Li J, Cooper J. Dynactin function in mitotic spindle positioning. Traffic. 2008;9:510-27 pubmed publisher
    ..Interactions among the subunits support a molecular model that resembles the current model for brain dynactin in many respects; however, three subunits at the pointed end of brain dynactin appear to be absent from yeast. ..
  5. Kardon J, Reck Peterson S, Vale R. Regulation of the processivity and intracellular localization of Saccharomyces cerevisiae dynein by dynactin. Proc Natl Acad Sci U S A. 2009;106:5669-74 pubmed publisher
    ..Instead, a segment of the coiled-coil of Nip100 is required for these activities. Our results directly demonstrate that dynactin increases the processivity of dynein through a mechanism independent of microtubule tethering. ..
  6. Scarfone I, Venturetti M, Hotz M, Lengefeld J, Barral Y, Piatti S. Asymmetry of the budding yeast Tem1 GTPase at spindle poles is required for spindle positioning but not for mitotic exit. PLoS Genet. 2015;11:e1004938 pubmed publisher
    ..Thus, asymmetry of the Bub2/Bfa1/Tem1 complex is crucial to control Kar9 distribution and spindle positioning during mitosis. ..
  7. Chao J, Wong A, Tavassoli S, Young B, Chruscicki A, Fang N, et al. Polarization of the endoplasmic reticulum by ER-septin tethering. Cell. 2014;158:620-32 pubmed publisher
    ..ER-septin tethering polarizes the ER into separate mother and bud domains, one function of which is to position the spindle in the mother until M phase by confining the spindle capture protein Num1 to the mother ER. ..
  8. Haarer B, Helfant A, Nelson S, Cooper J, Amberg D. Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck. Eukaryot Cell. 2007;6:797-807 pubmed
  9. Igarashi R, Suzuki M, Nogami S, Ohya Y. Molecular dissection of ARP1 regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae. Cell Struct Funct. 2005;30:57-67 pubmed
    ..The core of the dynactin complex is a backbone filament composed of monomers of an actin-related protein, Arp1, which is also involved in nuclear migration...
  10. Adames N, Oberle J, Cooper J. The surveillance mechanism of the spindle position checkpoint in yeast. J Cell Biol. 2001;153:159-68 pubmed
    ..In both wild-type and mutant cells, mitotic exit was preceded by loss of cytoplasmic microtubules from the neck. Thus, the spindle position checkpoint may monitor such interactions. ..
  11. Wang Y, Hu F, Elledge S. The Bfa1/Bub2 GAP complex comprises a universal checkpoint required to prevent mitotic exit. Curr Biol. 2000;10:1379-82 pubmed
    ..We propose that Bfa1/Bub2 checkpoint pathway functions as a universal checkpoint in G2/M that prevents CDK inactivation in response to cell-cycle delay in G2/M. ..