Gene Symbol: ARF3
Description: Arf family GTPase ARF3
Alias: ARL2, Arf family GTPase ARF3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Huang C, Liu Y, Tung L, Lin C, Lee F. Role for Arf3p in development of polarity, but not endocytosis, in Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:3834-47 pubmed
    ..C-Terminally green fluorescent protein-tagged Arf3, expressed from the endogenous promoter, exhibited a polarized localization to the cell periphery and buds, in a ..
  2. Tsai P, Lee S, Liu Y, Chu C, Chen K, Ho J, et al. Afi1p functions as an Arf3p polarization-specific docking factor for development of polarity. J Biol Chem. 2008;283:16915-27 pubmed publisher
    ..However, Afi1p is not required for targeting of Arf3p or Yel1p to the plasma membrane. Like arf3 mutant yeast, afi1 mutant yeast exhibited an abnormal budding pattern and partially delayed actin patch ..
  3. Lambert A, Perron M, Lavoie E, Pallotta D. The Saccharomyces cerevisiae Arf3 protein is involved in actin cable and cortical patch formation. FEMS Yeast Res. 2007;7:782-95 pubmed
    ..Profilin-deficient cells (pfy1Delta) have severe growth defects and lack actin cables. Overexpression of ARF3 restores actin cables and corrects growth defects in these cells...
  4. Zakrzewska E, Perron M, Laroche A, Pallotta D. A role for GEA1 and GEA2 in the organization of the actin cytoskeleton in Saccharomyces cerevisiae. Genetics. 2003;165:985-95 pubmed
    ..The ADP-ribosylation factor Arf3p may be a target of Gea1p/Gea2p, since overexpression of ARF3 partially suppressed the profilin-deficient phenotype and a deletion of ARF3 exacerbated the phenotype of a pfy1-..
  5. Costa R, Warren D, Ayscough K. Lsb5p interacts with actin regulators Sla1p and Las17p, ubiquitin and Arf3p to couple actin dynamics to membrane trafficking processes. Biochem J. 2005;387:649-58 pubmed
    ..We also demonstrate hitherto unknown interactions between Lsb5p and the active form of the yeast Arf3 protein, and with ubiquitin...
  6. Gillingham A, Munro S. Identification of a guanine nucleotide exchange factor for Arf3, the yeast orthologue of mammalian Arf6. PLoS ONE. 2007;2:e842 pubmed
    ..In this paper we describe the characterisation of a GEF for the yeast orthologue of Arf6, Arf3, which we have named Yel1 (yeast EFA6-like-1) using yeast genetics, fluorescence microscopy and in vitro nucleotide ..
  7. Smaczynska de Rooij I, Costa R, Ayscough K. Yeast Arf3p modulates plasma membrane PtdIns(4,5)P2 levels to facilitate endocytosis. Traffic. 2008;9:559-73 pubmed publisher
    ..Significantly, deletion of arf3 causes a reduction in PtdIns(4,5)P2 at the plasma membrane, while increased levels of active Arf3p, caused by ..
  8. Costa R, Ayscough K. Interactions between Sla1p, Lsb5p and Arf3p in yeast endocytosis. Biochem Soc Trans. 2005;33:1273-5 pubmed
    ..We show that Lsb5p can interact with yeast Arf3p (orthologous with mammalian Arf6) and we demonstrate a requirement for Arf3p expression in order to localize Lsb5p to the cell cortex. ..
  9. Hsu J, Lee F. Arf3p GTPase is a key regulator of Bud2p activation for invasive growth in Saccharomyces cerevisiae. Mol Biol Cell. 2013;24:2328-39 pubmed publisher
    ..Genetic analysis shows that deletion of BUD1 suppresses the defect of invasive growth in arf3? or bud2? cells. Lack of Arf3p, like that of Bud2p, causes the intracellular accumulation of Bud1p-GTP...

More Information


  1. Hsu J, Chen K, Lee F. Snf1/AMP-activated protein kinase activates Arf3p to promote invasive yeast growth via a non-canonical GEF domain. Nat Commun. 2015;6:7840 pubmed publisher
    ..Thus, our study reveals a novel mechanism for regulating cellular responses to energy deprivation, in particular invasive cell growth, through direct Arf activation by Snf1/AMPK. ..
  2. Lay D, L Grosshans B, Heid H, Gorgas K, Just W. Binding and functions of ADP-ribosylation factor on mammalian and yeast peroxisomes. J Biol Chem. 2005;280:34489-99 pubmed
    ..ScARF1 regulated this process in a positive manner, and ScARF3 regulated it in a negative manner. ..
  3. Lee F, Stevens L, Kao Y, Moss J, Vaughan M. Characterization of a glucose-repressible ADP-ribosylation factor 3 (ARF3) from Saccharomyces cerevisiae. J Biol Chem. 1994;269:20931-7 pubmed
    ..As yARF3 was not essential for cell viability and was not required for endoplasmic reticulum to Golgi protein transport, it may provide an opportunity to define an ARF function in another kind of vesicular trafficking. ..
  4. Tsvetanova N, Klass D, Salzman J, Brown P. Proteome-wide search reveals unexpected RNA-binding proteins in Saccharomyces cerevisiae. PLoS ONE. 2010;5: pubmed publisher
    ..Our results suggest that many more RBPs still remain to be identified and provide a set of candidates for further investigation. ..
  5. Gillingham A, Tong A, Boone C, Munro S. The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi. J Cell Biol. 2004;167:281-92 pubmed
    ..Tassin, C. Fedriani, and M. Bornens. 2004. Cell. 118:323-335). In contrast, we find that this region binds to the Golgi in a GRAB domain-dependent manner, suggesting that GMAP-210 may not link the Golgi to gamma-tubulin and centrosomes. ..
  6. Brett C, Plemel R, Lobingier B, Lobinger B, Vignali M, Fields S, et al. Efficient termination of vacuolar Rab GTPase signaling requires coordinated action by a GAP and a protein kinase. J Cell Biol. 2008;182:1141-51 pubmed publisher
    ..We propose that signal propagation through the Ypt7 pathway is controlled by integrated feedback and feed-forward loops. In this model, Yck3 enforces a requirement for the activated Rab in docking and fusion. ..
  7. Hanzal Bayer M, Linari M, Wittinghofer A. Properties of the interaction of Arf-like protein 2 with PDEdelta. J Mol Biol. 2005;350:1074-82 pubmed
    ..Here, we show by a variety of spectroscopic techniques that Arl2, unlike most other Ras-related proteins, has micromolar rather than picomolar affinity for nucleotides...
  8. Nithianantham S, Le S, Seto E, Jia W, Leary J, Corbett K, et al. Tubulin cofactors and Arl2 are cage-like chaperones that regulate the soluble αβ-tubulin pool for microtubule dynamics. elife. 2015;4: pubmed publisher
    ..Five conserved tubulin cofactors/chaperones and the Arl2 GTPase regulate α- and β-tubulin assembly into heterodimers and maintain the soluble tubulin pool in the ..
  9. Parrish W, Stefan C, Emr S. PtdIns(3)P accumulation in triple lipid-phosphatase-deletion mutants triggers lethal hyperactivation of the Rho1p/Pkc1p cell-integrity MAP kinase pathway. J Cell Sci. 2005;118:5589-601 pubmed
    ..Taken together, the results of this study indicated that aberrant Rho1p/Pkc1p signaling contributes to the lethal effects of PtdIns(3)P accumulation in cells deficient in PI 3-phosphatase activity. ..