Gene Symbol: ARF1
Description: Arf family GTPase ARF1
Alias: Arf family GTPase ARF1
Species: Saccharomyces cerevisiae S288c
Products:     ARF1

Top Publications

  1. van der Vaart A, Griffith J, Reggiori F. Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:2270-84 pubmed publisher
    ..Our data show that the Golgi complex plays a crucial role in supplying the lipid bilayers necessary for the biogenesis of double-membrane vesicles possibly through a new class of transport carriers or a new mechanism. ..
  2. Eugster A, Frigerio G, Dale M, Duden R. COP I domains required for coatomer integrity, and novel interactions with ARF and ARF-GAP. EMBO J. 2000;19:3905-17 pubmed
    ..Glo3p also interacts with intact coatomer in vitro. ..
  3. Spang A, Schekman R. Reconstitution of retrograde transport from the Golgi to the ER in vitro. J Cell Biol. 1998;143:589-99 pubmed
    ..Putative retrograde carriers (COPI vesicles) generated from Golgi-enriched membranes contain v-SNAREs as well as Emp47p as cargo...
  4. Chantalat S, Courbeyrette R, Senic Matuglia F, Jackson C, Goud B, Peyroche A. A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p. Mol Biol Cell. 2003;14:2357-71 pubmed
    ..Hence, we propose that Gmh1p acts as a positive Golgi-membrane partner for Gea function. These results are of general interest given the evolutionary conservation of both ARF-GEFs and the Gmh proteins. ..
  5. Lay D, L Grosshans B, Heid H, Gorgas K, Just W. Binding and functions of ADP-ribosylation factor on mammalian and yeast peroxisomes. J Biol Chem. 2005;280:34489-99 pubmed
    ..Although both ARF1 and ARF6 were found on peroxisomes, coatomer recruitment only depended on ARF1-GTP...
  6. Kahn R, Clark J, Rulka C, Stearns T, Zhang C, Randazzo P, et al. Mutational analysis of Saccharomyces cerevisiae ARF1. J Biol Chem. 1995;270:143-50 pubmed
    Wild type and eight point mutants of Saccharomyces cerevisiae ARF1 were expressed in yeast and bacteria to determine the roles of specific residues in in vivo and in vitro activities...
  7. Duden R, Hosobuchi M, Hamamoto S, Winey M, Byers B, Schekman R. Yeast beta- and beta'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic. J Biol Chem. 1994;269:24486-95 pubmed
    ..Genetic interactions connect sec27-1 and sec21-1 (coatomer gamma subunit) with the ARF1 and ARF2 genes and with the SEC22, BET1, and BOS1 genes, which encode membrane proteins involved in ER-to-Golgi ..
  8. Chen C, Graham T. An arf1Delta synthetic lethal screen identifies a new clathrin heavy chain conditional allele that perturbs vacuolar protein transport in Saccharomyces cerevisiae. Genetics. 1998;150:577-89 pubmed
    ..This genetic interaction between arf1 and chc1 provides in vivo evidence for a role for ARF in clathrin coat assembly...
  9. Spang A, Matsuoka K, Hamamoto S, Schekman R, Orci L. Coatomer, Arf1p, and nucleotide are required to bud coat protein complex I-coated vesicles from large synthetic liposomes. Proc Natl Acad Sci U S A. 1998;95:11199-204 pubmed
    ..We conclude that Arf1p-GTP and coatomer comprise the minimum apparatus necessary to create a COPI-coated vesicle...

More Information


  1. Poon P, Cassel D, Spang A, Rotman M, Pick E, Singer R, et al. Retrograde transport from the yeast Golgi is mediated by two ARF GAP proteins with overlapping function. EMBO J. 1999;18:555-64 pubmed
    ..The in vitro GTPase activity of the Saccharomyces cerevisiae ARF proteins Arf1 and Arf2 is stimulated by the yeast Gcs1 protein, and in vivo genetic interactions between arf and gcs1 mutations ..
  2. Walch Solimena C, Novick P. The yeast phosphatidylinositol-4-OH kinase pik1 regulates secretion at the Golgi. Nat Cell Biol. 1999;1:523-5 pubmed
  3. Stearns T, Willingham M, Botstein D, Kahn R. ADP-ribosylation factor is functionally and physically associated with the Golgi complex. Proc Natl Acad Sci U S A. 1990;87:1238-42 pubmed
    ..Yeast cells bearing an arf1 null mutation display a number of phenotypes suggesting a defect in the secretory pathway...
  4. Sanchatjate S, Schekman R. Chs5/6 complex: a multiprotein complex that interacts with and conveys chitin synthase III from the trans-Golgi network to the cell surface. Mol Biol Cell. 2006;17:4157-66 pubmed
    ..Our results suggest a model of protein sorting at the TGN that involves a peripheral, possibly coat, complex that includes multiple related copies of a specificity determining subunit. ..
  5. Valdivia R, Baggott D, Chuang J, Schekman R. The yeast clathrin adaptor protein complex 1 is required for the efficient retention of a subset of late Golgi membrane proteins. Dev Cell. 2002;2:283-94 pubmed
    ..Similarly, in AP-1 deficient cells, the resident TGN/early endosome syntaxin, Tlg1p, is missorted. We propose that clathrin and AP-1 act to recycle Chs3p and Tlg1p from the early endosome to the TGN. ..
  6. Stearns T, Kahn R, Botstein D, Hoyt M. ADP ribosylation factor is an essential protein in Saccharomyces cerevisiae and is encoded by two genes. Mol Cell Biol. 1990;10:6690-9 pubmed
    ..A gene (ARF1) encoding a protein homologous to mammalian ARF was recently cloned from Saccharomyces cerevisiae (Sewell and Kahn, ..
  7. Trautwein M, Dengjel J, Schirle M, Spang A. Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:5021-37 pubmed
    ..Hereby acts the SHE machinery in long-range mRNA transport, whereas COPI vesicles could act as short-range and localization vehicles. The endoplasmic reticulum (ER)-Golgi shuttle might be involved in concentrating mRNA at the ER. ..
  8. Trautwein M, Schindler C, Gauss R, Dengjel J, Hartmann E, Spang A. Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi. EMBO J. 2006;25:943-54 pubmed
    ..In addition, they are all at least partially localized to the TGN in a Chs5p-dependent manner. Most importantly, several ChAPs can interact physically with Chs3p. We propose that the ChAPs facilitate export of cargo out of the Golgi...
  9. Poon P, Wang X, Rotman M, Huber I, Cukierman E, Cassel D, et al. Saccharomyces cerevisiae Gcs1 is an ADP-ribosylation factor GTPase-activating protein. Proc Natl Acad Sci U S A. 1996;93:10074-7 pubmed
    ..The in vivo role of Gcs1 as an ARF GAP is substantiated by genetic interactions between mutations in the ARF1/ARF2 redundant pair of yeast ARF genes and a gcs1-null mutation; cells lacking both Gcs1 and Arf1 proteins are ..
  10. Zhang C, Cavenagh M, Kahn R. A family of Arf effectors defined as suppressors of the loss of Arf function in the yeast Saccharomyces cerevisiae. J Biol Chem. 1998;273:19792-6 pubmed
    ..a family of four genes (Suppressors of Arf ts, SAT) as high copy suppressors of a loss of function allele of ARF1 (arf1-3)...
  11. Yu X, Breitman M, Goldberg J. A structure-based mechanism for Arf1-dependent recruitment of coatomer to membranes. Cell. 2012;148:530-42 pubmed publisher
    ..structural basis for Arf-dependent recruitment of a vesicular coat to the membrane, we determined the structure of Arf1 bound to the ??-COP subcomplex of coatomer...
  12. Wang C, Hamamoto S, Orci L, Schekman R. Exomer: A coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast. J Cell Biol. 2006;174:973-83 pubmed
    ..Unlike other coats (e.g., coat protein complex I, II, and clathrin/adaptor protein complex), the exomer does not form buds or vesicles on liposomes...
  13. Demmel L, Beck M, Klose C, Schlaitz A, Gloor Y, Hsu P, et al. Nucleocytoplasmic shuttling of the Golgi phosphatidylinositol 4-kinase Pik1 is regulated by 14-3-3 proteins and coordinates Golgi function with cell growth. Mol Biol Cell. 2008;19:1046-61 pubmed publisher
    ..These data suggest a role of Pik1p nucleocytoplasmic shuttling in coordination of biosynthetic transport from the Golgi with nutrient signaling. ..
  14. Zhdankina O, Strand N, Redmond J, Boman A. Yeast GGA proteins interact with GTP-bound Arf and facilitate transport through the Golgi. Yeast. 2001;18:1-18 pubmed
    ..However, cells lacking GGA1 and GGA2 exhibit defects in invertase processing and CPY sorting, but not endocytosis. We conclude that yeast Gga proteins are effectors of Arf in yeast that facilitate traffic through the late Golgi. ..
  15. Moss J, Vaughan M. Molecules in the ARF orbit. J Biol Chem. 1998;273:21431-4 pubmed
  16. Richardson B, McDonold C, Fromme J. The Sec7 Arf-GEF is recruited to the trans-Golgi network by positive feedback. Dev Cell. 2012;22:799-810 pubmed publisher
    ..through the stable recruitment of Sec7 to membranes via its HDS1 domain by interaction with its product, activated Arf1. This interaction mediates localization of Sec7 to the TGN, because deletion of the HDS1 domain or mutation of the ..
  17. Liu K, Surendhran K, Nothwehr S, Graham T. P4-ATPase requirement for AP-1/clathrin function in protein transport from the trans-Golgi network and early endosomes. Mol Biol Cell. 2008;19:3526-35 pubmed publisher
    ..These results imply that Drs2p acts independently of coat recruitment to facilitate AP-1/clathrin-coated vesicle budding from the TGN. ..
  18. Rudge S, Cavenagh M, Kamath R, Sciorra V, Morris A, Kahn R, et al. ADP-Ribosylation factors do not activate yeast phospholipase Ds but are required for sporulation. Mol Biol Cell. 1998;9:2025-36 pubmed
    ADP-ribosylation factor (ARF) proteins in Saccharomyces cerevisiae are encoded by two genes, ARF1 and ARF2...
  19. Paczkowski J, Richardson B, Strassner A, Fromme J. The exomer cargo adaptor structure reveals a novel GTPase-binding domain. EMBO J. 2012;31:4191-203 pubmed publisher
    ..Exomer is recruited to the TGN by the Arf1 GTPase, a universally conserved trafficking regulator...
  20. Silva A, Xu X, Kim H, Fillingham J, Kislinger T, Mennella T, et al. The replication-independent histone H3-H4 chaperones HIR, ASF1, and RTT106 co-operate to maintain promoter fidelity. J Biol Chem. 2012;287:1709-18 pubmed publisher
    ..We propose that nucleosome shielding is the primary means to maintain promoter fidelity, and histone replacement is most efficiently mediated in yeast cells by a HIR/Asf1/H3-H4/Rtt106 pathway. ..
  21. Gillingham A, Tong A, Boone C, Munro S. The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi. J Cell Biol. 2004;167:281-92 pubmed
    ..Tassin, C. Fedriani, and M. Bornens. 2004. Cell. 118:323-335). In contrast, we find that this region binds to the Golgi in a GRAB domain-dependent manner, suggesting that GMAP-210 may not link the Golgi to gamma-tubulin and centrosomes. ..
  22. Park S, Hartnell L, Jackson C. Mutations in a highly conserved region of the Arf1p activator GEA2 block anterograde Golgi transport but not COPI recruitment to membranes. Mol Biol Cell. 2005;16:3786-99 pubmed
    We have identified an important functional region of the yeast Arf1 activator Gea2p upstream of the catalytic Sec7 domain and characterized a set of temperature-sensitive (ts) mutants with amino acid substitutions in this region...
  23. Sakane H, Yamamoto T, Tanaka K. The functional relationship between the Cdc50p-Drs2p putative aminophospholipid translocase and the Arf GAP Gcs1p in vesicle formation in the retrieval pathway from yeast early endosomes to the TGN. Cell Struct Funct. 2006;31:87-108 pubmed
    ..subunit of the Cdc50p-Drs2p putative aminophospholipid translocase, has been implicated in conjunction with the Arf1 signaling pathway in the formation of clathrin-coated vesicles (CCVs) from the TGN...
  24. Chen C, Ingram M, Rosal P, Graham T. Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function. J Cell Biol. 1999;147:1223-36 pubmed
    ..These observations indicate a novel role for a P-type ATPase in late Golgi function and suggest a possible link between membrane asymmetry and clathrin function at the Golgi complex. ..
  25. Richardson B, Halaby S, Gustafson M, Fromme J. The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase. elife. 2016;5: pubmed publisher
    ..We find that the DCB/HUS domain amplifies the ability of Sec7 to activate Arf1 on the membrane surface by facilitating membrane insertion of the Arf1 amphipathic helix...
  26. Zhang C, Bowzard J, Greene M, Anido A, Stearns K, Kahn R. Genetic interactions link ARF1, YPT31/32 and TRS130. Yeast. 2002;19:1075-86 pubmed
    A genetic screen for synthetic lethal interactions with arf1(-) identified a recessive mutation in TRS130, one of 10 components in the trafficking protein particle (TRAPP) complex (Sacher et al., 2000)...
  27. Schindler C, Spang A. Interaction of SNAREs with ArfGAPs precedes recruitment of Sec18p/NSF. Mol Biol Cell. 2007;18:2852-63 pubmed
  28. Webster M, McCaffery J, COHEN FIX O. Vesicle trafficking maintains nuclear shape in Saccharomyces cerevisiae during membrane proliferation. J Cell Biol. 2010;191:1079-88 pubmed publisher
    ..Moreover, flares may facilitate maintenance of a constant nuclear/cell volume ratio in the face of altered membrane proliferation. ..
  29. Gall W, Geething N, Hua Z, Ingram M, Liu K, Chen S, et al. Drs2p-dependent formation of exocytic clathrin-coated vesicles in vivo. Curr Biol. 2002;12:1623-7 pubmed
    ..An arf1 synthetic lethal screen identified DRS2/SWA3 along with a clathrin heavy-chain conditional allele (chc1-5/swa5-1) ..
  30. Paczkowski J, Fromme J. Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor. Methods Mol Biol. 2016;1496:41-53 pubmed publisher
    ..The small GTPase Arf1 and related proteins have the ability to remodel membranes by insertion of an amphipathic helix into the membrane...
  31. Moss J, Vaughan M. Activation of toxin ADP-ribosyltransferases by eukaryotic ADP-ribosylation factors. Mol Cell Biochem. 1999;193:153-7 pubmed
    ..There are now five Sec7 domain proteins known to have GEP activity toward class I ARFs. It remains to be determined whether there are other Sec7 domain proteins that are GEPs for ARFs 4, 5, or 6. ..
  32. Li X, Rivas M, Fang M, Marchena J, Mehrotra B, Chaudhary A, et al. Analysis of oxysterol binding protein homologue Kes1p function in regulation of Sec14p-dependent protein transport from the yeast Golgi complex. J Cell Biol. 2002;157:63-77 pubmed
  33. Chen S, Cai H, Park S, Menon S, Jackson C, Ferro Novick S. Trs65p, a subunit of the Ypt1p GEF TRAPPII, interacts with the Arf1p exchange factor Gea2p to facilitate COPI-mediated vesicle traffic. Mol Biol Cell. 2011;22:3634-44 pubmed publisher
    ..In support of this proposal, we show that TRAPPII is more soluble in an arf1? mutant.
  34. Zhang C, Bowzard J, Anido A, Kahn R. Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and distinct functions. Yeast. 2003;20:315-30 pubmed
    ..a conserved, cysteine-rich, ARF GAP domain that share the ability to suppress the conditional growth defect of the arf1-3 mutant...
  35. Liu Y, Kahn R, Prestegard J. Dynamic structure of membrane-anchored Arf*GTP. Nat Struct Mol Biol. 2010;17:876-81 pubmed publisher
    ..We report a high-resolution NMR structure for full-length myristoylated yeast (Saccharomyces cerevisiae) Arf1 in complex with a membrane mimic...
  36. Kilchert C, Weidner J, Prescianotto Baschong C, Spang A. Defects in the secretory pathway and high Ca2+ induce multiple P-bodies. Mol Biol Cell. 2010;21:2624-38 pubmed publisher
    ..Unexpectedly, in Saccharomyces cerevisiae, mutants of the small GTPase Arf1 and various secretory pathway mutants induced a significant increase in PB number, compared with PB induction by ..
  37. Spang A, Herrmann J, Hamamoto S, Schekman R. The ADP ribosylation factor-nucleotide exchange factors Gea1p and Gea2p have overlapping, but not redundant functions in retrograde transport from the Golgi to the endoplasmic reticulum. Mol Biol Cell. 2001;12:1035-45 pubmed
    ..In contrast, Sec7p, which is required for protein transport within the Golgi, is not required for retrograde protein trafficking. ..
  38. Dechant R, Saad S, Ibáñez A, Peter M. Cytosolic pH regulates cell growth through distinct GTPases, Arf1 and Gtr1, to promote Ras/PKA and TORC1 activity. Mol Cell. 2014;55:409-21 pubmed publisher
    ..V-ATPase interacts with two distinct GTPases, Arf1 and Gtr1, which are required for Ras and TORC1 activation, respectively...
  39. Beck R, Sun Z, Adolf F, Rutz C, Bassler J, Wild K, et al. Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proc Natl Acad Sci U S A. 2008;105:11731-6 pubmed publisher
    The GTPase Arf1 is considered as a molecular switch that regulates binding and release of coat proteins that polymerize on membranes to form transport vesicles...
  40. Liu Y, Huang C, Huang K, Lee F. Role for Gcs1p in regulation of Arl1p at trans-Golgi compartments. Mol Biol Cell. 2005;16:4024-33 pubmed
    ..Our data are consistent with the conclusion that Arl1p moves in a dynamic equilibrium between trans-Golgi and cytosol, and the release of Arl1p from membranes in cells requires the hydrolysis of bound GTP, which is accelerated by Gcs1p. ..
  41. Naticchia M, Brown H, Garcia F, Lamade A, Justice S, Herrin R, et al. Bifunctional electrophiles cross-link thioredoxins with redox relay partners in cells. Chem Res Toxicol. 2013;26:490-7 pubmed publisher
    ..Taken together, our results indicate that bifunctional electrophiles potentially disrupt redox homeostasis in yeast and human cells by forming cross-linked complexes between thioredoxins and their redox partners. ..
  42. Ackema K, Hench J, Böckler S, Wang S, Sauder U, Mergentaler H, et al. The small GTPase Arf1 modulates mitochondrial morphology and function. EMBO J. 2014;33:2659-75 pubmed publisher
    The small GTPase Arf1 plays critical roles in membrane traffic by initiating the recruitment of coat proteins and by modulating the activity of lipid-modifying enzymes...
  43. Chen K, Tsai P, Hsu J, Hsu H, Fang C, Chang L, et al. Syt1p promotes activation of Arl1p at the late Golgi to recruit Imh1p. J Cell Sci. 2010;123:3478-89 pubmed publisher
    ..These findings reveal that activation of Arl1p is regulated in part by Syt1p, and imply that Arl1p activation, by using more than one GEF, exerts distinct biological activities at the Golgi compartment. ..
  44. Singer Krüger B, Lasić M, Bürger A, Hausser A, Pipkorn R, Wang Y. Yeast and human Ysl2p/hMon2 interact with Gga adaptors and mediate their subcellular distribution. EMBO J. 2008;27:1423-35 pubmed publisher
    ..Because activated Arl1p is part of the network that binds Gga2p, Arf and Arf-like GTPases may interact in a regulatory cascade. ..
  45. Rein U, Andag U, Duden R, Schmitt H, Spang A. ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat. J Cell Biol. 2002;157:395-404 pubmed
    ..The mechanisms by which v-SNAREs interact with COPI and COPII coat proteins seem to be different and may play a key role in determining specificity in vesicle budding...
  46. Gall W, Higginbotham M, Chen C, Ingram M, Cyr D, Graham T. The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast. Curr Biol. 2000;10:1349-58 pubmed
    ..The Saccharomyces cerevisiae SWA2 gene, previously identified in a synthetic lethal screen with arf1, was cloned and found to encode a protein with a carboxy-terminal DnaJ domain which is homologous to that of ..
  47. Sewell J, Kahn R. Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins. Proc Natl Acad Sci U S A. 1988;85:4620-4 pubmed
    ..These results demonstrate that ARF is a GTP-binding protein with structural features of both the ras and the trimeric GTP-binding protein families. ..
  48. Kim D, Massey T, Sacher M, Pypaert M, Ferro Novick S. Sgf1p, a new component of the Sec34p/Sec35p complex. Traffic. 2001;2:820-30 pubmed
    ..Although an earlier study suggested that Sec34p (Grd20p) is not required for protein secretion, we show here that the sec34-2 and sec35-1 mutations lead to a pleiotropic block in the secretion of all proteins into the growth medium. ..
  49. Hsu J, Chang L, Jang L, Huang C, Lee F. The N-terminus of Vps74p is essential for the retention of glycosyltransferases in the Golgi but not for the modulation of apical polarized growth in Saccharomyces cerevisiae. PLoS ONE. 2013;8:e74715 pubmed publisher
    ..Thus, we propose that Vps74p may use different domains to interact with specific effectors thereby differentially modulating a variety of cellular functions. ..
  50. Boman A, Salo P, Hauglund M, Strand N, Rensink S, Zhdankina O. ADP-ribosylation factor (ARF) interaction is not sufficient for yeast GGA protein function or localization. Mol Biol Cell. 2002;13:3078-95 pubmed
    ..Whereas mammalian GGAs must interact with ARF to localize to and function at the Golgi, interaction between yeast Ggas and Arf plays a minor role in Gga localization and function. ..
  51. Yahara N, Sato K, Nakano A. The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J Cell Sci. 2006;119:2604-12 pubmed
    ..We previously isolated eight temperature-sensitive (ts) mutants of the yeast ARF1 gene, which showed allele-specific defects in protein transport, and classified them into three groups of ..
  52. McDonold C, Fromme J. Four GTPases differentially regulate the Sec7 Arf-GEF to direct traffic at the trans-golgi network. Dev Cell. 2014;30:759-67 pubmed publisher
    ..The Golgi Arf-GEF Sec7 and the homologous BIG1/2 proteins are effectors of the Arf1 and Arl1 GTPases...
  53. Thomas L, Fromme J. GTPase cross talk regulates TRAPPII activation of Rab11 homologues during vesicle biogenesis. J Cell Biol. 2016;215:499-513 pubmed
    ..We also uncover a direct role for Arf1, a distinct GTPase, in recruiting TRAPPII to anionic membranes...