Gene Symbol: APL5
Alias: YKS4, Apl5p
Species: Saccharomyces cerevisiae S288c
Cowles C, Odorizzi G, Payne G, Emr S. The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole. Cell. 1997;91:109-18 pubmed
..This study, therefore, provides evidence that the AP-3 complex functions in cargo-selective protein transport from the Golgi to the vacuole/lysosome. ..
Rehling P, Darsow T, Katzmann D, Emr S. Formation of AP-3 transport intermediates requires Vps41 function. Nat Cell Biol. 1999;1:346-53 pubmed
..Vps41 binds to the AP-3 delta-adaptin subunit, suggesting that they function together in the formation of ALP pathway transport intermediates at the late Golgi. ..
Angers C, Merz A. HOPS interacts with Apl5 at the vacuole membrane and is required for consumption of AP-3 transport vesicles. Mol Biol Cell. 2009;20:4563-74 pubmed publisher
..We now show that although the AP-3 delta subunit, Apl5, binds Vps41 directly, this interaction occurs preferentially within the context of the HOPS docking complex...
Darsow T, Katzmann D, Cowles C, Emr S. Vps41p function in the alkaline phosphatase pathway requires homo-oligomerization and interaction with AP-3 through two distinct domains. Mol Biol Cell. 2001;12:37-51 pubmed
..These data indicate that a homo-oligomeric form of Vps41p is required for the formation of ALP containing vesicles at the Golgi complex via interactions with AP-3. ..
Mahfouz H, Ragnini Wilson A, Venditti R, De Matteis M, Wilson C. Mutational analysis of the yeast TRAPP subunit Trs20p identifies roles in endocytic recycling and sporulation. PLoS ONE. 2012;7:e41408 pubmed publisher
..The phenotypes of different mutants appear to be separable suggesting that the mutations affect the function of Trs20 in different TRAPP complexes. ..
Berger A, Salazar G, Styers M, Newell Litwa K, Werner E, Maue R, et al
. The subcellular localization of the Niemann-Pick Type C proteins depends on the adaptor complex AP-3. J Cell Sci. 2007;120:3640-52 pubmed
..These results provide information about how the NP-C proteins are targeted to their sites of action and illustrate the possibility that defective sorting of the NP-C proteins along the endocytic route can alter cellular cholesterol. ..
Cabrera M, Langemeyer L, Mari M, Rethmeier R, Orban I, Perz A, et al
. Phosphorylation of a membrane curvature-sensing motif switches function of the HOPS subunit Vps41 in membrane tethering. J Cell Biol. 2010;191:845-59 pubmed publisher
..this motif is inserted into the lipid bilayer and masks the binding motif for the ? subunit of the AP-3 complex, Apl5, without affecting the Vps41 function in endosome-vacuole fusion...
Webster M, McCaffery J, COHEN FIX O. Vesicle trafficking maintains nuclear shape in Saccharomyces cerevisiae during membrane proliferation. J Cell Biol. 2010;191:1079-88 pubmed publisher
..Moreover, flares may facilitate maintenance of a constant nuclear/cell volume ratio in the face of altered membrane proliferation. ..
Toshima J, Nishinoaki S, Sato Y, Yamamoto W, Furukawa D, Siekhaus D, et al
. Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. Nat Commun. 2014;5:3498 pubmed publisher
..We propose that the endocytic traffic branches into two routes to reach the vacuole: a Rab5-dependent VPS pathway and a Rab5-independent AP-3-mediated pathway. ..