APC1

Summary

Gene Symbol: APC1
Description: anaphase promoting complex subunit 1
Alias: anaphase promoting complex subunit 1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Grossberger R, Gieffers C, Zachariae W, Podtelejnikov A, Schleiffer A, Nasmyth K, et al. Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex. J Biol Chem. 1999;274:14500-7 pubmed
    ..DOC domains may therefore be important for reactions catalyzed by several types of ubiquitin-protein ligases. ..
  2. Zachariae W, Shin T, Galova M, Obermaier B, Nasmyth K. Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae. Science. 1996;274:1201-4 pubmed
    ..Two additional subunits of the budding yeast APC were identified: The largest subunit, encoded by the APC1 gene, is conserved between fungi and vertebrates and shows similarity to BIMEp from Aspergillus nidulans...
  3. Benanti J, Matyskiela M, Morgan D, Toczyski D. Functionally distinct isoforms of Cik1 are differentially regulated by APC/C-mediated proteolysis. Mol Cell. 2009;33:581-90 pubmed publisher
    ..These results demonstrate a coupling of transcriptional regulation and APC/C-mediated proteolysis. ..
  4. Passmore L, Booth C, Venien Bryan C, Ludtke S, Fioretto C, Johnson L, et al. Structural analysis of the anaphase-promoting complex reveals multiple active sites and insights into polyubiquitylation. Mol Cell. 2005;20:855-66 pubmed
    ..Our data suggest that multimerisation and/or the presence of multiple active sites facilitates the APC/C's ability to elongate polyubiquitin chains. ..
  5. Carroll C, Enquist Newman M, Morgan D. The APC subunit Doc1 promotes recognition of the substrate destruction box. Curr Biol. 2005;15:11-8 pubmed
    ..Doc1 contributes to recognition of the substrate destruction box by the APC. This function of Doc1 is necessary for efficient substrate proteolysis in vivo. ..
  6. Schwickart M, Havlis J, Habermann B, Bogdanova A, Camasses A, Oelschlaegel T, et al. Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27. Mol Cell Biol. 2004;24:3562-76 pubmed
    ..Accordingly, Swm1/Apc13 is required for ubiquitin ligase activity in vitro and for the timely execution of APC/C-dependent cell cycle events in vivo. ..
  7. Carroll C, Morgan D. The Doc1 subunit is a processivity factor for the anaphase-promoting complex. Nat Cell Biol. 2002;4:880-7 pubmed
    ..Doc1-related domains are found in mechanistically distinct ubiquitin-ligase enzymes and may generally stimulate ubiquitination by contributing to substrate-enzyme affinity. ..
  8. Passmore L, McCormack E, Au S, Paul A, Willison K, Harper J, et al. Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition. EMBO J. 2003;22:786-96 pubmed
    ..These results imply that Doc1p/Apc10 may play a role to regulate the binding of specific substrates, similar to that of the coactivators. ..
  9. Hall M, Torres M, Schroeder G, Borchers C. Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex. J Biol Chem. 2003;278:16698-705 pubmed
    ..Mnd2 interacted strongly with Cdc23, Apc5, and Apc1 when coexpressed in an in vitro transcription/translation reaction...

More Information

Publications24

  1. Passmore L, Barford D, Harper J. Purification and assay of the budding yeast anaphase-promoting complex. Methods Enzymol. 2005;398:195-219 pubmed
    ..Activity assays toward several endogenous substrates, including Clb2 and Pds1, are described. In addition, methods for observation of APC-coactivator and APC-substrate complexes by native gel electrophoresis are described. ..
  2. Thornton B, Ng T, Matyskiela M, Carroll C, Morgan D, Toczyski D. An architectural map of the anaphase-promoting complex. Genes Dev. 2006;20:449-60 pubmed
    ..We found that the largest subunit, Apc1, serves as a scaffold that associates independently with two separable subcomplexes, one that contains Apc2 (Cullin)..
  3. Hein J, Nilsson J. Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint. EMBO Rep. 2014;15:264-72 pubmed publisher
    ..We thus show that stable MCC association with the APC/C is critical for a functional SAC. ..
  4. Oelschlaegel T, Schwickart M, Matos J, Bogdanova A, Camasses A, Havlis J, et al. The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. Cell. 2005;120:773-88 pubmed
    ..In vitro, Mnd2 inhibits ubiquitination of Pds1 by APC/C-Ama1 but not by other APC/C holo-enzymes. We conclude that chromosome segregation in meiosis depends on the selective inhibition of a meiosis-specific form of the APC/C. ..
  5. Nougarede R, Della Seta F, Zarzov P, Schwob E. Hierarchy of S-phase-promoting factors: yeast Dbf4-Cdc7 kinase requires prior S-phase cyclin-dependent kinase activation. Mol Cell Biol. 2000;20:3795-806 pubmed
    ..Linking the origin-bound DDK to the tightly regulated S-CDK in a dependent sequence of events may ensure that DNA replication initiates only at the right time and place. ..
  6. Ostapenko D, Solomon M. Anaphase promoting complex-dependent degradation of transcriptional repressors Nrm1 and Yhp1 in Saccharomyces cerevisiae. Mol Biol Cell. 2011;22:2175-84 pubmed publisher
    ..Therefore, in addition to its known functions, APC-mediated targeting of Nrm1 and Yhp1 coordinates transcription of multiple genes in G1 with other cell cycle events. ..
  7. Buschhorn B, Petzold G, Galova M, Dube P, Kraft C, Herzog F, et al. Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1. Nat Struct Mol Biol. 2011;18:6-13 pubmed publisher
    ..We show that Doc1 interacts with Cdc27, Cdc16 and Apc1 and is located in the vicinity of the cullin-RING module Apc2-Apc11 in the inner cavity of the APC/C...
  8. da Fonseca P, Kong E, Zhang Z, Schreiber A, Williams M, Morris E, et al. Structures of APC/C(Cdh1) with substrates identify Cdh1 and Apc10 as the D-box co-receptor. Nature. 2011;470:274-8 pubmed publisher
    ..Our results rationalize the contribution of both co-activator and core APC/C subunits to D-box recognition and provide a structural framework for understanding mechanisms of substrate recognition and catalysis by the APC/C. ..
  9. Rossio V, Michimoto T, Sasaki T, Ohbayashi I, Kikuchi Y, Yoshida S. Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint. J Cell Sci. 2013;126:4396-405 pubmed publisher
    ..Because the Cdc55-binding proteins Zds1 and Zds2 inhibit both nuclear accumulation of Cdc55 and SAC activity, we propose that spatial control of PP2A by Zds1 family proteins is important for tight control of SAC and mitotic progression. ..
  10. Qin L, GuimarĂ£es D, Melesse M, Hall M. Substrate Recognition by the Cdh1 Destruction Box Receptor Is a General Requirement for APC/CCdh1-mediated Proteolysis. J Biol Chem. 2016;291:15564-74 pubmed publisher
    ..Our results suggest that binding to the D-box receptor may be a shared requirement for recognition and processing of all Cdh1 substrates. ..
  11. Torres M, Borchers C. Mitotic phosphorylation of the anaphase-promoting complex inhibitory subunit Mnd2 is necessary for efficient progression through meiosis i. J Biol Chem. 2007;282:17351-62 pubmed
    ..Taken together, these data suggest that mitotic phosphorylation of Mnd2 is necessary for APC-mediated progression beyond the first meiotic nuclear division. ..
  12. Blethrow J, Tang C, Deng C, Krutchinsky A. Modular mass spectrometric tool for analysis of composition and phosphorylation of protein complexes. PLoS ONE. 2007;2:e358 pubmed
  13. Page A, Aneliunas V, Lamb J, Hieter P. In vivo characterization of the nonessential budding yeast anaphase-promoting complex/cyclosome components Swm1p, Mnd2p and Apc9p. Genetics. 2005;170:1045-62 pubmed
    ..Furthermore, the role of Apc9p in APC/C-dependent ubiquitination may be limited to the proteolysis of a select number of substrates. ..
  14. Camasses A, Bogdanova A, Shevchenko A, Zachariae W. The CCT chaperonin promotes activation of the anaphase-promoting complex through the generation of functional Cdc20. Mol Cell. 2003;12:87-100 pubmed
    ..We propose that folding of the Cdc20 family of APC/C activators is an essential and evolutionary conserved function of the CCT chaperonin. ..
  15. Zachariae W, Shevchenko A, Andrews P, Ciosk R, Galova M, Stark M, et al. Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science. 1998;279:1216-9 pubmed
    ..Apc2p is similar to the cullin Cdc53p, which is a subunit of the ubiquitin-protein ligase complex SCFCdc4 required for the initiation of DNA replication. ..