Gene Symbol: AMS1
Species: Saccharomyces cerevisiae S288c
Yuga M, Gomi K, Klionsky D, Shintani T. Aspartyl aminopeptidase is imported from the cytoplasm to the vacuole by selective autophagy in Saccharomyces cerevisiae. J Biol Chem. 2011;286:13704-13 pubmed publisher
..mechanism for constitutive transport of the specific vacuolar hydrolases aminopeptidase I (Ape1) and ?-mannosidase (Ams1); this process is called the cytoplasm to vacuole targeting (Cvt) pathway...
Watanabe Y, Noda N, Honbou K, Suzuki K, Sakai Y, Ohsumi Y, et al
. Crystallization of Saccharomyces cerevisiae alpha-mannosidase, a cargo protein of the Cvt pathway. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65:571-3 pubmed publisher
Saccharomyces cerevisiae alpha-mannosidase (Ams1) is a cargo protein that is transported to the vacuole by the cytoplasm-to-vacuole targeting (Cvt) pathway during conditions of growth and by autophagy during conditions of starvation...
Yoshihisa T, Ohsumi Y, Anraku Y. Solubilization and purification of alpha-mannosidase, a marker enzyme of vacuolar membranes in Saccharomyces cerevisiae. J Biol Chem. 1988;263:5158-63 pubmed
..It suggested that proteolytic conversion of the 107-kDa polypeptide occurs to form the 73- and 31-kDa polypeptides and leads to formation of isoforms of the enzyme. ..
Chantret I, Frénoy J, Moore S. Free-oligosaccharide control in the yeast Saccharomyces cerevisiae: roles for peptide:N-glycanase (Png1p) and vacuolar mannosidase (Ams1p). Biochem J. 2003;373:901-8 pubmed
..Use of an ams1 Delta strain deficient in the vacuolar alpha-mannosidase revealed this enzyme to be responsible for the slow growth-..
White E, Cowan C, Cande W, Kaback D. In vivo analysis of synaptonemal complex formation during yeast meiosis. Genetics. 2004;167:51-63 pubmed
..ZIP1::GFP also facilitated the enrichment of fluorescent SC and the identification of meiosis-specific proteins by MALDI-TOF mass spectroscopy. ..
Mochida K, Ohsumi Y, Nakatogawa H. Hrr25 phosphorylates the autophagic receptor Atg34 to promote vacuolar transport of Î±-mannosidase under nitrogen starvation conditions. FEBS Lett. 2014;588:3862-9 pubmed publisher
In Saccharomyces cerevisiae, under nitrogen-starvation conditions, the Î±-mannosidase Ams1 is recognized by the autophagic receptor Atg34 and transported into the vacuole, where it functions as an active enzyme...
Shintani T, Huang W, Stromhaug P, Klionsky D. Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway. Dev Cell. 2002;3:825-37 pubmed
Suzuki K, Kondo C, Morimoto M, Ohsumi Y. Selective transport of alpha-mannosidase by autophagic pathways: identification of a novel receptor, Atg34p. J Biol Chem. 2010;285:30019-25 pubmed publisher
..Atg34p interacted with Ams1p, Atg11p, and Atg8p using distinct domains. Homo-oligomerized Ams1p bound to the Ams1-binding domain of Atg34p; this binding was important for the formation of a higher order complex named the Ams1 ..
Yoshihisa T, Anraku Y. Nucleotide sequence of AMS1, the structure gene of vacuolar alpha-mannosidase of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1989;163:908-15 pubmed
b>AMS1, a structure gene of the vacuolar membrane alpha-mannosidase of Saccharomyces cerevisiae, has been characterized and found to encode both constituent polypeptides of the enzyme, a 107 kDa polypeptide and a 73 kDa polypeptide...