Gene Symbol: AMS1
Description: alpha-mannosidase
Alias: alpha-mannosidase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Yuga M, Gomi K, Klionsky D, Shintani T. Aspartyl aminopeptidase is imported from the cytoplasm to the vacuole by selective autophagy in Saccharomyces cerevisiae. J Biol Chem. 2011;286:13704-13 pubmed publisher
    ..mechanism for constitutive transport of the specific vacuolar hydrolases aminopeptidase I (Ape1) and ?-mannosidase (Ams1); this process is called the cytoplasm to vacuole targeting (Cvt) pathway...
  2. Watanabe Y, Noda N, Honbou K, Suzuki K, Sakai Y, Ohsumi Y, et al. Crystallization of Saccharomyces cerevisiae alpha-mannosidase, a cargo protein of the Cvt pathway. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65:571-3 pubmed publisher
    Saccharomyces cerevisiae alpha-mannosidase (Ams1) is a cargo protein that is transported to the vacuole by the cytoplasm-to-vacuole targeting (Cvt) pathway during conditions of growth and by autophagy during conditions of starvation...
  3. Yoshihisa T, Ohsumi Y, Anraku Y. Solubilization and purification of alpha-mannosidase, a marker enzyme of vacuolar membranes in Saccharomyces cerevisiae. J Biol Chem. 1988;263:5158-63 pubmed
    ..It suggested that proteolytic conversion of the 107-kDa polypeptide occurs to form the 73- and 31-kDa polypeptides and leads to formation of isoforms of the enzyme. ..
  4. Chantret I, Frénoy J, Moore S. Free-oligosaccharide control in the yeast Saccharomyces cerevisiae: roles for peptide:N-glycanase (Png1p) and vacuolar mannosidase (Ams1p). Biochem J. 2003;373:901-8 pubmed
    ..Use of an ams1 Delta strain deficient in the vacuolar alpha-mannosidase revealed this enzyme to be responsible for the slow growth-..
  5. White E, Cowan C, Cande W, Kaback D. In vivo analysis of synaptonemal complex formation during yeast meiosis. Genetics. 2004;167:51-63 pubmed
    ..ZIP1::GFP also facilitated the enrichment of fluorescent SC and the identification of meiosis-specific proteins by MALDI-TOF mass spectroscopy. ..
  6. Mochida K, Ohsumi Y, Nakatogawa H. Hrr25 phosphorylates the autophagic receptor Atg34 to promote vacuolar transport of α-mannosidase under nitrogen starvation conditions. FEBS Lett. 2014;588:3862-9 pubmed publisher
    In Saccharomyces cerevisiae, under nitrogen-starvation conditions, the α-mannosidase Ams1 is recognized by the autophagic receptor Atg34 and transported into the vacuole, where it functions as an active enzyme...
  7. Shintani T, Huang W, Stromhaug P, Klionsky D. Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway. Dev Cell. 2002;3:825-37 pubmed
  8. Suzuki K, Kondo C, Morimoto M, Ohsumi Y. Selective transport of alpha-mannosidase by autophagic pathways: identification of a novel receptor, Atg34p. J Biol Chem. 2010;285:30019-25 pubmed publisher
    ..Atg34p interacted with Ams1p, Atg11p, and Atg8p using distinct domains. Homo-oligomerized Ams1p bound to the Ams1-binding domain of Atg34p; this binding was important for the formation of a higher order complex named the Ams1 ..
  9. Yoshihisa T, Anraku Y. Nucleotide sequence of AMS1, the structure gene of vacuolar alpha-mannosidase of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1989;163:908-15 pubmed
    b>AMS1, a structure gene of the vacuolar membrane alpha-mannosidase of Saccharomyces cerevisiae, has been characterized and found to encode both constituent polypeptides of the enzyme, a 107 kDa polypeptide and a 73 kDa polypeptide...

More Information


  1. Watanabe Y, Noda N, Kumeta H, Suzuki K, Ohsumi Y, Inagaki F. Selective transport of alpha-mannosidase by autophagic pathways: structural basis for cargo recognition by Atg19 and Atg34. J Biol Chem. 2010;285:30026-33 pubmed publisher
    In the yeast Saccharomyces cerevisiae, a precursor form of aminopeptidase I (prApe1) and ?-mannosidase (Ams1) are selectively transported to the vacuole through the cytoplasm-to-vacuole targeting pathway under vegetative conditions and ..
  2. Umekawa M, Ujihara M, Makishima K, Yamamoto S, Takematsu H, Wakayama M. The signaling pathways underlying starvation-induced upregulation of α-mannosidase Ams1 in Saccharomyces cerevisiae. Biochim Biophys Acta. 2016;1860:1192-201 pubmed publisher
    ..In Saccharomyces cerevisiae, α-mannosidase Ams1 is known to play a role in catabolism of N-linked free oligosaccharides in the cytosol...
  3. Bertipaglia C, Schneider S, Jakobi A, Tarafder A, Bykov Y, Picco A, et al. Higher-order assemblies of oligomeric cargo receptor complexes form the membrane scaffold of the Cvt vesicle. EMBO Rep. 2016;17:1044-60 pubmed publisher
    ..8 Å X-ray and negative stain EM structure, as well as the secondary cargo Ams1 with a 6.3 Å cryo-EM structure...