Gene Symbol: ADH1
Description: alcohol dehydrogenase ADH1
Alias: ADC1, alcohol dehydrogenase ADH1
Species: Saccharomyces cerevisiae S288c
Products:     ADH1

Top Publications

  1. Ida Y, Furusawa C, Hirasawa T, Shimizu H. Stable disruption of ethanol production by deletion of the genes encoding alcohol dehydrogenase isozymes in Saccharomyces cerevisiae. J Biosci Bioeng. 2012;113:192-5 pubmed publisher
    ..The decrease in ethanol production by ADH1 deletion alone could be partially compensated by the upregulation of other isozyme genes, while the deletion of all ..
  2. Williamson V, Bennetzen J, Young E, Nasmyth K, Hall B. Isolation of the structural gene for alcohol dehydrogenase by genetic complementation in yeast. Nature. 1980;283:214-6 pubmed
  3. Gao Q, Madian A, Liu X, Adamec J, Regnier F. Coupling protein complex analysis to peptide based proteomics. J Chromatogr A. 2010;1217:7661-8 pubmed publisher
  4. Huang K, Filarsky M, Padula M, Raftery M, Herbert B, Wilkins M. Micropreparative fractionation of the complexome by blue native continuous elution electrophoresis. Proteomics. 2009;9:2494-502 pubmed publisher
    ..We illustrate the utility of the technique in the analysis of Saccharomyces cerevisiae cellular lysate. ..
  5. Markossian K, Golub N, Khanova H, Levitsky D, Poliansky N, Muranov K, et al. Mechanism of thermal aggregation of yeast alcohol dehydrogenase I: role of intramolecular chaperone. Biochim Biophys Acta. 2008;1784:1286-93 pubmed publisher
    ..Thermodynamic parameters for thermal denaturation of yADH have been calculated from the differential scanning calorimetry data. ..
  6. Men L, Wang Y. The oxidation of yeast alcohol dehydrogenase-1 by hydrogen peroxide in vitro. J Proteome Res. 2007;6:216-25 pubmed
    ..The oxidation of Cys43 might be responsible for the inactivation of the enzyme upon H2O2 treatment. ..
  7. Laadan B, Wallace Salinas V, Carlsson Ã, Almeida J, RÃ¥dström P, Gorwa Grauslund M. Furaldehyde substrate specificity and kinetics of Saccharomyces cerevisiae alcohol dehydrogenase 1 variants. Microb Cell Fact. 2014;13:112 pubmed publisher
    ..In the present study, site-directed mutagenesis of both native and mutated ADH1 genes was performed in order to identify the key amino acids involved in this substrate shift, resulting in Adh1p-..
  8. Bird A, Gordon M, Eide D, Winge D. Repression of ADH1 and ADH3 during zinc deficiency by Zap1-induced intergenic RNA transcripts. EMBO J. 2006;25:5726-34 pubmed
    ..We demonstrate that during zinc starvation, Zap1 is required for the repression of ADH1 expression. ADH1 encodes the major zinc-dependent alcohol dehydrogenase that is utilized during fermentation...
  9. Kaplan Y, Kupiec M. A role for the yeast cell cycle/splicing factor Cdc40 in the G1/S transition. Curr Genet. 2007;51:123-40 pubmed
    ..Finally, we discuss possible mechanisms of suppression by the cDNAs that imply cell cycle regulation by apparently unrelated processes, such as splicing, translation initiation and glycolysis. ..

More Information


  1. Biver S, Portetelle D, Vandenbol M. Multicopy suppression screen in a Saccharomyces cerevisiae strain lacking the Rab GTPase-activating protein Msb3p. Biotechnol Lett. 2011;33:123-9 pubmed publisher
    ..Three genes (ADH1, RNT1, and SUI1) were found to suppress the CdCl(2) sensitivity of the msb3 strain and three others (YAP6, ZEO1, ..
  2. Lahudkar S, Durairaj G, Uprety B, Bhaumik S. A novel role for Cet1p mRNA 5'-triphosphatase in promoter proximal accumulation of RNA polymerase II in Saccharomyces cerevisiase. Genetics. 2014;196:161-76 pubmed publisher
    ..Here, we show that the accumulation of RNA polymerase II at the promoter proximal site of ADH1 is significantly enhanced in the absence of Cet1p. Similar results are also found at other genes...
  3. Raj S, Ramaswamy S, Plapp B. Yeast alcohol dehydrogenase structure and catalysis. Biochemistry. 2014;53:5791-803 pubmed publisher
    Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (ADH1) is the constitutive enzyme that reduces acetaldehyde to ethanol during the fermentation of glucose. ADH1 is a homotetramer of subunits with 347 amino acid residues...
  4. Thomson J, Gaucher E, Burgan M, De Kee D, Li T, Aris J, et al. Resurrecting ancestral alcohol dehydrogenases from yeast. Nat Genet. 2005;37:630-5 pubmed
    ..Pyruvate loses carbon dioxide to produce acetaldehyde, which is reduced by alcohol dehydrogenase 1 (Adh1) to ethanol, which accumulates...
  5. Tramonti A, Saliola M. Glucose 6-phosphate and alcohol dehydrogenase activities are components of dynamic macromolecular depots structures. Biochim Biophys Acta. 2015;1850:1120-30 pubmed publisher
    ..belonging to glycolysis, pentose phosphate, folding and stress response pathways, were associated with G6PDH and Adh1 putative complexes and that a number of these proteins were identical in either network in both yeasts...
  6. Smith M, Des Etages S, Snyder M. Microbial synergy via an ethanol-triggered pathway. Mol Cell Biol. 2004;24:3874-84 pubmed
    ..was due to a diffusible factor that was shown to be ethanol by chemical assays and evaluation of strains lacking ADH1, ADH3, and ADH5, as all three genes are involved in ethanol production by yeast...
  7. Chen C, Porubleva L, Shearer G, Svrakic M, Holden L, Dover J, et al. Associating protein activities with their genes: rapid identification of a gene encoding a methylglyoxal reductase in the yeast Saccharomyces cerevisiae. Yeast. 2003;20:545-54 pubmed
    ..Our approach should be useful for identifying many of the genes that encode the metabolic pathway enzymes that have not been associated with a gene (about 275 in S. cerevisiae, by our estimate). ..
  8. van Loon A, Young E. Intracellular sorting of alcohol dehydrogenase isoenzymes in yeast: a cytosolic location reflects absence of an amino-terminal targeting sequence for the mitochondrion. EMBO J. 1986;5:161-5 pubmed
    ..We conclude that ADHII is a cytosolic protein because it lacks an amino-terminal targeting sequence for the mitochondrion and that ADHIII is a mitochondrial protein because it contains a mitochondrial targeting sequence. ..
  9. Reverter Branchat G, Cabiscol E, Tamarit J, Sorolla M, Angeles de la Torre M, Ros J. Chronological and replicative life-span extension in Saccharomyces cerevisiae by increased dosage of alcohol dehydrogenase 1. Microbiology. 2007;153:3667-76 pubmed
    Alcohol dehydrogenase 1 (Adh1)p catalyses the conversion of acetaldehyde to ethanol, regenerating NAD+. In Saccharomyces cerevisiae, Adh1p is oxidatively modified during ageing and, consequently, its activity becomes reduced...
  10. Calvo O, Manley J. The transcriptional coactivator PC4/Sub1 has multiple functions in RNA polymerase II transcription. EMBO J. 2005;24:1009-20 pubmed
    ..Our data provide evidence that Rna15 and Sub1 are present along the length of several genes and that Sub1 facilitates elongation by influencing enzymes that modify RNAP II. ..
  11. Jayakody L, Horie K, Hayashi N, Kitagaki H. Engineering redox cofactor utilization for detoxification of glycolaldehyde, a key inhibitor of bioethanol production, in yeast Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2013;97:6589-600 pubmed publisher
    ..Glycolaldehyde is one of the strongest fermentation inhibitors and we developed a tolerant strain by overexpressing ADH1 encoding an NADH-dependent reductase; however, its recovery was partial...
  12. DERMODY J, Buratowski S. Leo1 subunit of the yeast paf1 complex binds RNA and contributes to complex recruitment. J Biol Chem. 2010;285:33671-9 pubmed publisher
    ..Together, these results suggest that association of Paf1C with RNA stabilizes its localization at actively transcribed regions where it influences chromatin structure. ..
  13. Ganzhorn A, Green D, Hershey A, Gould R, Plapp B. Kinetic characterization of yeast alcohol dehydrogenases. Amino acid residue 294 and substrate specificity. J Biol Chem. 1987;262:3754-61 pubmed
    ..The higher activity is due to tighter binding of the longer chain alcohols and to more rapid hydrogen transfer. ..
  14. Kusano M, Sakai Y, Kato N, Yoshimoto H, Sone H, Tamai Y. Hemiacetal dehydrogenation activity of alcohol dehydrogenases in Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 1998;62:1956-61 pubmed
    ..Both ADH and methyl formate synthesizing activities were sharply reduced in the delta adh1 delta adh2 mutant. The ADH1 and ADH2 genes encode the major Adh proteins in S. cerevisiae...
  15. Plapp B, Charlier H, Ramaswamy S. Mechanistic implications from structures of yeast alcohol dehydrogenase complexed with coenzyme and an alcohol. Arch Biochem Biophys. 2016;591:35-42 pubmed publisher
    ..5 Ã… from the zinc. Subunit B may represent an intermediate in the mechanism after coenzyme and alcohol bind and before the conformation changes to the closed form and the alcohol oxygen binds to the zinc and displaces Glu-67. ..
  16. Yu K, Jung J, Ramzi A, Kim S, Park C, Han S. Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae. Appl Biochem Biotechnol. 2012;166:856-65 pubmed publisher
    ..cerevisiae was successfully accomplished. The overexpression of an alcohol dehydrogenase (adh1) and a pyruvate decarboxylase (pdc1) caused an increase in growth rate and glycerol consumption under fermentative ..
  17. Tokuhiro K, Ishida N, Nagamori E, Saitoh S, Onishi T, Kondo A, et al. Double mutation of the PDC1 and ADH1 genes improves lactate production in the yeast Saccharomyces cerevisiae expressing the bovine lactate dehydrogenase gene. Appl Microbiol Biotechnol. 2009;82:883-90 pubmed publisher
    ..We conducted double disruption of the pyruvate decarboxylase 1 (PDC1) and alcohol dehydrogenase 1 (ADH1) genes in a S. cerevisiae strain by replacing them with the bovine L: -ldh gene...
  18. Hou Z, Danzer J, Mendoza L, Bose M, Muller U, Williams B, et al. Phylogenetic conservation and homology modeling help reveal a novel domain within the budding yeast heterochromatin protein Sir1. Mol Cell Biol. 2009;29:687-702 pubmed publisher
    ..Thus, the Sir1 N terminus exhibited both positive and negative roles in the formation of a Sir1-ORC silencing complex. This functional duality might contribute to Sir1's selectivity for silencer-bound ORCs in vivo. ..
  19. Laadan B, Almeida J, Radstrom P, Hahn Hägerdal B, Gorwa Grauslund M. Identification of an NADH-dependent 5-hydroxymethylfurfural-reducing alcohol dehydrogenase in Saccharomyces cerevisiae. Yeast. 2008;25:191-8 pubmed publisher
    ..Increased biomass production and HMF conversion rate were achieved in a CEN.PK S. cerevisiae strain overexpressing the mutated ADH1 gene.
  20. Sagot I, Schaeffer J, Daignan Fornier B. Guanylic nucleotide starvation affects Saccharomyces cerevisiae mother-daughter separation and may be a signal for entry into quiescence. BMC Cell Biol. 2005;6:24 pubmed
  21. Muratani M, Kung C, Shokat K, Tansey W. The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing. Cell. 2005;120:887-99 pubmed
    ..We propose that Gal4 ubiquitylation and destruction are required for initiation-competent transcription complexes to transition to fully mature elongating complexes capable of appropriate mRNA processing. ..
  22. Dickinson J, Salgado L, Hewlins M. The catabolism of amino acids to long chain and complex alcohols in Saccharomyces cerevisiae. J Biol Chem. 2003;278:8028-34 pubmed