Gene Symbol: ADE3
Description: trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3
Alias: trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Koren A, Ben Aroya S, Steinlauf R, Kupiec M. Pitfalls of the synthetic lethality screen in Saccharomyces cerevisiae: an improved design. Curr Genet. 2003;43:62-9 pubmed
    ..Frequently, the plasmid carries the ADE3 gene and mutants are recognized as red colonies that fail to produce sectors...
  2. Joo Y, Kim J, Baek J, Seong K, Han K, Song J, et al. Cooperative regulation of ADE3 transcription by Gcn4p and Bas1p in Saccharomyces cerevisiae. Eukaryot Cell. 2009;8:1268-77 pubmed publisher
    ..The ADE3 gene is known to encode a crucial one-carbon regulon enzyme, tetrahydrofolate synthase, which is involved in the ..
  3. Hürlimann H, Laloo B, Simon Kayser B, Saint Marc C, Coulpier F, Lemoine S, et al. Physiological and toxic effects of purine intermediate 5-amino-4-imidazolecarboxamide ribonucleotide (AICAR) in yeast. J Biol Chem. 2011;286:30994-1002 pubmed publisher
    ..Finally, we show that AICAR/SAICAR concentrations vary under physiological conditions known to modulate transcription of the purine and phosphate pathway genes. ..
  4. Beaufour M, Godin F, Vallee B, Cadene M, Bénédetti H. Interaction proteomics suggests a new role for the Tfs1 protein in yeast. J Proteome Res. 2012;11:3211-8 pubmed publisher
    ..Integration of these results with known functional partners of Tfs1p shows that two subnetworks meet through the Tfs1p node, suggesting that it may act as a bridge between cell signaling and intermediate metabolism in yeast. ..
  5. Blazeck J, Miller J, Pan A, Gengler J, Holden C, Jamoussi M, et al. Metabolic engineering of Saccharomyces cerevisiae for itaconic acid production. Appl Microbiol Biotechnol. 2014;98:8155-64 pubmed publisher
    ..In this manner, we successfully identify three non-obvious genetic targets (?ade3 ?bna2 ?tes1) that successively improve itaconic acid titer...
  6. Saint Marc C, Hürlimann H, Daignan Fornier B, Pinson B. Serine hydroxymethyltransferase: a key player connecting purine, folate and methionine metabolism in Saccharomyces cerevisiae. Curr Genet. 2015;61:633-40 pubmed publisher
    ..In addition, we uncovered a new methionine auxotrophy for the ade3 bas1 double mutant that can be rescued by overexpressing the SHM2 gene...
  7. Lomax C, Gross T, Woods R. New mutant types at the ade3 locus of Saccharomyces cerevisiae. J Bacteriol. 1971;107:1-7 pubmed
    Six mutants, allelic to ade3, were isolated after mutagenic treatment of a prototrophic strain of yeast. All six grow on medium supplemented with adenine alone and four respond to histidine...
  8. Song J, Rabinowitz J. Function of yeast cytoplasmic C1-tetrahydrofolate synthase. Proc Natl Acad Sci U S A. 1993;90:2636-40 pubmed
    The protein product of the ADE3 gene of the yeast Saccharomyces cerevisiae has been identified as the cytoplasmic trifunctional C1-tetrahydrofolate (THF) synthase, which possesses 10-formyl-THF synthetase (EC 6.3.4...
  9. McNeil J, Bognar A, Pearlman R. In vivo analysis of folate coenzymes and their compartmentation in Saccharomyces cerevisiae. Genetics. 1996;142:371-81 pubmed
    ..SHMTm)], SHM2 [cytoplasmic SHMT (SHMTc)], MIS1 [mitochondrial C1-tetrahydrofolate synthase (C1-THFSm)], ADE3 [cytoplasmic C1-THF synthase (C1-THFSc)], GCV1 [glycine cleavage system (GCV) protein T], and the GLY1 (involved in ..

More Information


  1. Nigavekar S, Cannon J. Characterization of genes that are synthetically lethal with ade3 or leu2 in Saccharomyces cerevisiae. Yeast. 2002;19:115-22 pubmed
    ..Frequently, an ade2 ade3 colony-sectoring assay is used to screen for synthetic lethal mutants...
  2. Yang Y, Meier U. Genetic interaction between a chaperone of small nucleolar ribonucleoprotein particles and cytosolic serine hydroxymethyltransferase. J Biol Chem. 2003;278:23553-60 pubmed
    ..mutation of both cytosolic serine hydroxymethyltransferase (SHM2) and one-carbon tetrahydrofolate synthase (ADE3) was required to achieve synthetic lethality with srp40Delta...
  3. Rébora K, Laloo B, Daignan Fornier B. Revisiting purine-histidine cross-pathway regulation in Saccharomyces cerevisiae: a central role for a small molecule. Genetics. 2005;170:61-70 pubmed
    ..Finally, we revisit the histidine auxotrophy of ade3 or ade16 ade17 mutants...
  4. Huang K, Filarsky M, Padula M, Raftery M, Herbert B, Wilkins M. Micropreparative fractionation of the complexome by blue native continuous elution electrophoresis. Proteomics. 2009;9:2494-502 pubmed publisher
    ..We illustrate the utility of the technique in the analysis of Saccharomyces cerevisiae cellular lysate. ..