ACT1

Summary

Gene Symbol: ACT1
Description: actin
Alias: ABY1, END7, actin
Species: Saccharomyces cerevisiae S288c
Products:     ACT1

Top Publications

  1. Galarneau L, Nourani A, Boudreault A, Zhang Y, Heliot L, Allard S, et al. Multiple links between the NuA4 histone acetyltransferase complex and epigenetic control of transcription. Mol Cell. 2000;5:927-37 pubmed
    ..We have identified three novel NuA4 subunits: Act3/Arp4, an actin-related protein implicated in epigenetic control of transcription, Act1, and Epl1, a protein homologous to ..
  2. Munn A, Stevenson B, Geli M, Riezman H. end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae. Mol Biol Cell. 1995;6:1721-42 pubmed
    ..Interestingly, localization of actin patches on the plasma membrane is affected in each of the mutants...
  3. Nourani A, Doyon Y, Utley R, Allard S, Lane W, Cote J. Role of an ING1 growth regulator in transcriptional activation and targeted histone acetylation by the NuA4 complex. Mol Cell Biol. 2001;21:7629-40 pubmed
    ..They also suggest that regulation of mammalian cell proliferation by p53-dependent transcriptional activation functions through recruitment of an ING1-containing histone acetyltransferase complex. ..
  4. Li R. Bee1, a yeast protein with homology to Wiscott-Aldrich syndrome protein, is critical for the assembly of cortical actin cytoskeleton. J Cell Biol. 1997;136:649-58 pubmed
    ..homology to Wiskott-Aldrich syndrome protein (WASP), a human protein that may link signaling pathways to the actin cytoskeleton...
  5. Goode B, Wong J, Butty A, Peter M, McCormack A, Yates J, et al. Coronin promotes the rapid assembly and cross-linking of actin filaments and may link the actin and microtubule cytoskeletons in yeast. J Cell Biol. 1999;144:83-98 pubmed
    Coronin is a highly conserved actin-associated protein that until now has had unknown biochemical activities...
  6. Pruyne D, Bretscher A. Polarization of cell growth in yeast. J Cell Sci. 2000;113 ( Pt 4):571-85 pubmed
    The actin cytoskeleton provides the structural basis for cell polarity in Saccharomyces cerevisiae as well as most other eukaryotes...
  7. Sandrock T, O Dell J, Adams A. Allele-specific suppression by formation of new protein-protein interactions in yeast. Genetics. 1997;147:1635-42 pubmed
    Yeast fimbrin is encoded by the SAC6 gene, mutations of which suppress temperature-sensitive mutations in the actin gene (ACT1)...
  8. Mattila P, Quintero Monzon O, Kugler J, Moseley J, Almo S, Lappalainen P, et al. A high-affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of Srv2/cyclase-associated protein. Mol Biol Cell. 2004;15:5158-71 pubmed
    Cyclase-associated protein (CAP), also called Srv2 in Saccharomyces cerevisiae, is a conserved actin monomer-binding protein that promotes cofilin-dependent actin turnover in vitro and in vivo...
  9. Pring M, Evangelista M, Boone C, Yang C, Zigmond S. Mechanism of formin-induced nucleation of actin filaments. Biochemistry. 2003;42:486-96 pubmed
    ..of the yeast formin Bni1 containing the FH1FH2 domains increases the rate of filament nucleation from pure G-actin [Pruyne et al. (2002) Science 297, 612-615]...

More Information

Publications63

  1. Honts J, Sandrock T, Brower S, O Dell J, Adams A. Actin mutations that show suppression with fimbrin mutations identify a likely fimbrin-binding site on actin. J Cell Biol. 1994;126:413-22 pubmed
    b>Actin interacts with a large number of different proteins that modulate its assembly and mediate its functions. One such protein is the yeast actin-binding protein Sac6p, which is homologous to vertebrate fimbrin (Adams, A. E. M., D...
  2. Drubin D, Miller K, Botstein D. Yeast actin-binding proteins: evidence for a role in morphogenesis. J Cell Biol. 1988;107:2551-61 pubmed
    Three yeast actin-binding proteins were identified using yeast actin filaments as an affinity matrix...
  3. Pruyne D, Evangelista M, Yang C, Bi E, Zigmond S, Bretscher A, et al. Role of formins in actin assembly: nucleation and barbed-end association. Science. 2002;297:612-5 pubmed
    Nucleation of branched actin filaments by the Arp2/3 complex is a conserved process in eukaryotic cells, yet the source of unbranched actin filaments has remained obscure...
  4. Reck Peterson S, Tyska M, Novick P, Mooseker M. The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors. J Cell Biol. 2001;153:1121-6 pubmed
    ..Additional support for the idea that Myo2p is a nonprocessive motor comes from actin cosedimentation assays, which show that Myo2p has a low affinity for F-actin in the presence of ATP and Ca(2+), ..
  5. Wolven A, Belmont L, Mahoney N, Almo S, Drubin D. In vivo importance of actin nucleotide exchange catalyzed by profilin. J Cell Biol. 2000;150:895-904 pubmed
    The actin monomer-binding protein, profilin, influences the dynamics of actin filaments in vitro by suppressing nucleation, enhancing nucleotide exchange on actin, and promoting barbed-end assembly...
  6. Lappalainen P, Fedorov E, Fedorov A, Almo S, Drubin D. Essential functions and actin-binding surfaces of yeast cofilin revealed by systematic mutagenesis. EMBO J. 1997;16:5520-30 pubmed
    Cofilin stimulates actin filament turnover in vivo. The phenotypes of twenty yeast cofilin mutants generated by systematic mutagenesis were determined...
  7. Wendland B, McCaffery J, Xiao Q, Emr S. A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15. J Cell Biol. 1996;135:1485-500 pubmed
    ..Phalloidin labeling of filamentous actin revealed profound defects in the actin cytoskeleton in both dim mutants...
  8. Karpova T, Tatchell K, Cooper J. Actin filaments in yeast are unstable in the absence of capping protein or fimbrin. J Cell Biol. 1995;131:1483-93 pubmed
    Many actin-binding proteins affect filament assembly in vitro and localize with actin in vivo, but how their molecular actions contribute to filament assembly in vivo is not understood well...
  9. Liu H, Bretscher A. Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton. Cell. 1989;57:233-42 pubmed
    ..Indirect immunofluorescence microscopy reveals that tropomyosin is localized with actin cables in wild-type cells...
  10. Shen X, Mizuguchi G, Hamiche A, Wu C. A chromatin remodelling complex involved in transcription and DNA processing. Nature. 2000;406:541-4 pubmed
    ..These results indicate that chromatin remodelling driven by the Ino80 ATPase may be connected to transcription as well as DNA damage repair. ..
  11. Adams A, Botstein D, Drubin D. A yeast actin-binding protein is encoded by SAC6, a gene found by suppression of an actin mutation. Science. 1989;243:231-3 pubmed
    The protein encoded by SAC6, a gene that can mutate to suppress a temperature-sensitive defect in the yeast actin gene, has been identified as a 67-kilodalton actin-binding protein (ABP 67) that associates with all identifiable actin ..
  12. Okada K, Ravi H, Smith E, Goode B. Aip1 and cofilin promote rapid turnover of yeast actin patches and cables: a coordinated mechanism for severing and capping filaments. Mol Biol Cell. 2006;17:2855-68 pubmed
    Rapid turnover of actin structures is required for dynamic remodeling of the cytoskeleton and cell morphogenesis, but the mechanisms driving actin disassembly are poorly defined...
  13. Mulholland J, Preuss D, Moon A, Wong A, Drubin D, Botstein D. Ultrastructure of the yeast actin cytoskeleton and its association with the plasma membrane. J Cell Biol. 1994;125:381-91 pubmed
    We characterized the yeast actin cytoskeleton at the ultrastructural level using immunoelectron microscopy. Anti-actin antibodies primarily labeled dense, patchlike cortical structures and cytoplasmic cables...
  14. Goodman A, Goode B, Matsudaira P, Fink G. The Saccharomyces cerevisiae calponin/transgelin homolog Scp1 functions with fimbrin to regulate stability and organization of the actin cytoskeleton. Mol Biol Cell. 2003;14:2617-29 pubmed
    Calponins and transgelins are members of a conserved family of actin-associated proteins widely expressed from yeast to humans...
  15. Skruzny M, Brach T, Ciuffa R, Rybina S, Wachsmuth M, Kaksonen M. Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis. Proc Natl Acad Sci U S A. 2012;109:E2533-42 pubmed
    Dynamic actin filaments are a crucial component of clathrin-mediated endocytosis when endocytic proteins cannot supply enough energy for vesicle budding...
  16. Gourlay C, Carpp L, Timpson P, Winder S, Ayscough K. A role for the actin cytoskeleton in cell death and aging in yeast. J Cell Biol. 2004;164:803-9 pubmed
    ..Here we report a role for the actin cytoskeleton both in cell death and in promoting longevity...
  17. Shen X. Preparation and analysis of the INO80 complex. Methods Enzymol. 2004;377:401-12 pubmed
  18. Amberg D, Zahner J, Mulholland J, Pringle J, Botstein D. Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites. Mol Biol Cell. 1997;8:729-53 pubmed
    A search for Saccharomyces cerevisiae proteins that interact with actin in the two-hybrid system and a screen for mutants that affect the bipolar budding pattern identified the same gene, AIP3/BUD6...
  19. Freeman N, Chen Z, Horenstein J, Weber A, Field J. An actin monomer binding activity localizes to the carboxyl-terminal half of the Saccharomyces cerevisiae cyclase-associated protein. J Biol Chem. 1995;270:5680-5 pubmed
    ..We present evidence showing that CAP sequesters monomeric actin (Kd in the range of 0.5-5 microM), decreasing actin incorporation into actin filaments...
  20. Rodal A, Tetreault J, Lappalainen P, Drubin D, Amberg D. Aip1p interacts with cofilin to disassemble actin filaments. J Cell Biol. 1999;145:1251-64 pubmed
    b>Actin interacting protein 1 (Aip1) is a conserved component of the actin cytoskeleton first identified in a two-hybrid screen against yeast actin...
  21. Karpova T, Reck Peterson S, Elkind N, Mooseker M, Novick P, Cooper J. Role of actin and Myo2p in polarized secretion and growth of Saccharomyces cerevisiae. Mol Biol Cell. 2000;11:1727-37 pubmed
    We examined the role of the actin cytoskeleton in secretion in Saccharomyces cerevisiae with the use of several quantitative assays, including time-lapse video microscopy of cell surface growth in individual living cells...
  22. Chernova T, Romanyuk A, Karpova T, Shanks J, Ali M, Moffatt N, et al. Prion induction by the short-lived, stress-induced protein Lsb2 is regulated by ubiquitination and association with the actin cytoskeleton. Mol Cell. 2011;43:242-52 pubmed publisher
    ..with Lsb2 ubiquitination increase prion induction, while a mutation eliminating association of Lsb2 with the actin cytoskeleton blocks its aggregation and prion-inducing ability...
  23. Suarez C, Roland J, Boujemaa Paterski R, Kang H, McCullough B, Reymann A, et al. Cofilin tunes the nucleotide state of actin filaments and severs at bare and decorated segment boundaries. Curr Biol. 2011;21:862-8 pubmed publisher
    b>Actin-based motility demands the spatial and temporal coordination of numerous regulatory actin-binding proteins (ABPs), many of which bind with affinities that depend on the nucleotide state of actin filament...
  24. Shen X, Ranallo R, Choi E, Wu C. Involvement of actin-related proteins in ATP-dependent chromatin remodeling. Mol Cell. 2003;12:147-55 pubmed
    b>Actin-related proteins (Arps) and conventional actin are enigmatic components of many chromatin-remodeling enzyme complexes...
  25. Moon A, Janmey P, Louie K, Drubin D. Cofilin is an essential component of the yeast cortical cytoskeleton. J Cell Biol. 1993;120:421-35 pubmed
    We have biochemically identified the Saccharomyces cerevisiae homologue of the mammalian actin binding protein cofilin...
  26. Fenn S, Breitsprecher D, Gerhold C, Witte G, Faix J, Hopfner K. Structural biochemistry of nuclear actin-related proteins 4 and 8 reveals their interaction with actin. EMBO J. 2011;30:2153-66 pubmed publisher
    Nuclear actin and actin-related proteins (Arps) are integral components of various chromatin-remodelling complexes...
  27. Adams A, Botstein D, Drubin D. Requirement of yeast fimbrin for actin organization and morphogenesis in vivo. Nature. 1991;354:404-8 pubmed
    The SAC6 gene was found by suppression of a yeast actin mutation. Its protein product, Sac6p (previously referred to as ABP67), was independently isolated by actin-filament affinity chromatography and colocalizes with actin in vivo...
  28. Eads J, Mahoney N, Vorobiev S, Bresnick A, Wen K, Rubenstein P, et al. Structure determination and characterization of Saccharomyces cerevisiae profilin. Biochemistry. 1998;37:11171-81 pubmed
    ..The interactions of yeast and human platelet profilins with rabbit skeletal muscle actin were characterized by titration microcalorimetry, fluorescence titrations, and nucleotide exchange kinetics...
  29. Winder S, Jess T, Ayscough K. SCP1 encodes an actin-bundling protein in yeast. Biochem J. 2003;375:287-95 pubmed
    The association of F-actin (filamentous actin) with a large number of binding proteins is essential for cellular function...
  30. Mizuguchi G, Shen X, Landry J, Wu W, Sen S, Wu C. ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex. Science. 2004;303:343-8 pubmed
    ..These findings define a previously unknown role for the adenosine triphosphate-dependent chromatin remodeling machinery. ..
  31. Zoladek T, Vaduva G, Hunter L, Boguta M, Go B, Martin N, et al. Mutations altering the mitochondrial-cytoplasmic distribution of Mod5p implicate the actin cytoskeleton and mRNA 3' ends and/or protein synthesis in mitochondrial delivery. Mol Cell Biol. 1995;15:6884-94 pubmed
    ..mdp2 can be suppressed by ACT1, the actin-encoding gene. The actin cytoskeleton organization is also aberrant in mdp2 cells. MDP2 is the same as VRP1 (S. F...
  32. Hill K, Catlett N, Weisman L. Actin and myosin function in directed vacuole movement during cell division in Saccharomyces cerevisiae. J Cell Biol. 1996;135:1535-49 pubmed
    ..cytoskeletal components that function in this process, vacuole inheritance was examined in a collection of actin mutants. Six strains were identified as being defective in vacuole inheritance...
  33. Novick P, Botstein D. Phenotypic analysis of temperature-sensitive yeast actin mutants. Cell. 1985;40:405-16 pubmed
    The consequences of two different mutations in the single essential actin structural gene of yeast (Saccharomyces cerevisiae) were studied...
  34. Belmont L, Drubin D. The yeast V159N actin mutant reveals roles for actin dynamics in vivo. J Cell Biol. 1998;142:1289-99 pubmed
    b>Actin with a Val 159 to Asn mutation (V159N) forms actin filaments that depolymerize slowly because of a failure to undergo a conformational change after inorganic phosphate release...
  35. Goode B, Rodal A, Barnes G, Drubin D. Activation of the Arp2/3 complex by the actin filament binding protein Abp1p. J Cell Biol. 2001;153:627-34 pubmed
    The actin-related protein (Arp) 2/3 complex plays a central role in assembly of actin networks...
  36. Goode B, Drubin D, Lappalainen P. Regulation of the cortical actin cytoskeleton in budding yeast by twinfilin, a ubiquitous actin monomer-sequestering protein. J Cell Biol. 1998;142:723-33 pubmed
    Here we describe the identification of a novel 37-kD actin monomer binding protein in budding yeast. This protein, which we named twinfilin, is composed of two cofilin-like regions...
  37. Evangelista M, Blundell K, Longtine M, Chow C, Adames N, Pringle J, et al. Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis. Science. 1997;276:118-22 pubmed
    ..response, bni1 mutants showed defects both in polarized morphogenesis and in reorganization of the underlying actin cytoskeleton...
  38. Kilmartin J, Adams A. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces. J Cell Biol. 1984;98:922-33 pubmed
    The distribution of actin and tubulin during the cell cycle of the budding yeast Saccharomyces was mapped by immunofluorescence using fixed cells from which the walls had been removed by digestion...
  39. Madania A, Dumoulin P, Grava S, Kitamoto H, Schärer Brodbeck C, Soulard A, et al. The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex. Mol Biol Cell. 1999;10:3521-38 pubmed
    ..Las17p/Bee1p has been shown to be important for actin patch assembly and actin polymerization. Here we show that Las17p interacts with the Arp2/3 complex...
  40. McCullough B, Grintsevich E, Chen C, Kang H, Hutchison A, Henn A, et al. Cofilin-linked changes in actin filament flexibility promote severing. Biophys J. 2011;101:151-9 pubmed publisher
    The actin regulatory protein, cofilin, increases the bending and twisting elasticity of actin filaments and severs them...
  41. Haarer B, Petzold A, Brown S. Mutational analysis of yeast profilin. Mol Cell Biol. 1993;13:7864-73 pubmed
    We have mutated two regions within the yeast profilin gene in an effort to functionally dissect the roles of actin and phosphatidylinositol 4,5-bisphosphate (PIP2) binding in profilin function...
  42. Gandhi M, Achard V, Blanchoin L, Goode B. Coronin switches roles in actin disassembly depending on the nucleotide state of actin. Mol Cell. 2009;34:364-74 pubmed publisher
    Rapid and polarized turnover of actin networks is essential for motility, endocytosis, cytokinesis, and other cellular processes...
  43. Szerlong H, Hinata K, Viswanathan R, Erdjument Bromage H, Tempst P, Cairns B. The HSA domain binds nuclear actin-related proteins to regulate chromatin-remodeling ATPases. Nat Struct Mol Biol. 2008;15:469-76 pubmed publisher
    We identify the helicase-SANT-associated (HSA) domain as the primary binding platform for nuclear actin-related proteins (ARPs) and actin...
  44. Auger A, Galarneau L, Altaf M, Nourani A, Doyon Y, Utley R, et al. Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants. Mol Cell Biol. 2008;28:2257-70 pubmed publisher
    ..Our results identified the key central subunit for the structure and functions of the NuA4 histone acetyltransferase complex and functionally linked this activity with the histone variant H2AZ from yeast to human cells. ..
  45. Robertson A, Allwood E, Smith A, Gardiner F, Costa R, Winder S, et al. The WASP homologue Las17 activates the novel actin-regulatory activity of Ysc84 to promote endocytosis in yeast. Mol Biol Cell. 2009;20:1618-28 pubmed publisher
    b>Actin plays an essential role in many eukaryotic cellular processes, including motility, generation of polarity, and membrane trafficking...
  46. Farah M, Amberg D. Conserved actin cysteine residues are oxidative stress sensors that can regulate cell death in yeast. Mol Biol Cell. 2007;18:1359-65 pubmed
    b>Actin's functional complexity makes it a likely target of oxidative stress but also places it in a prime position to coordinate the response to oxidative stress...
  47. Holtzman D, Wertman K, Drubin D. Mapping actin surfaces required for functional interactions in vivo. J Cell Biol. 1994;126:423-32 pubmed
    An in vivo strategy to identify amino acids of actin required for functional interactions with actin-binding proteins was developed...
  48. Quintero Monzon O, Jonasson E, Bertling E, Talarico L, Chaudhry F, Sihvo M, et al. Reconstitution and dissection of the 600-kDa Srv2/CAP complex: roles for oligomerization and cofilin-actin binding in driving actin turnover. J Biol Chem. 2009;284:10923-34 pubmed publisher
    ..protein is expressed in virtually all plant, animal, and fungal organisms and has a conserved role in promoting actin depolymerizing factor/cofilin-mediated actin turnover...
  49. Pruyne D, Schott D, Bretscher A. Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast. J Cell Biol. 1998;143:1931-45 pubmed
    The actin cytoskeleton in budding yeast consists of cortical patches and cables, both of which polarize toward regions of cell growth. Tropomyosin localizes specifically to actin cables and not cortical patches...
  50. Wang T, Bretscher A. The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast. Mol Biol Cell. 1995;6:1011-24 pubmed
    Microfilaments are required for polarized growth and morphogenesis in Saccharomyces cerevisiae. To accomplish this, actin cables and patches are redistributed during the cell cycle to direct secretory components to appropriate sites for ..
  51. Geli M, Lombardi R, Schmelzl B, Riezman H. An intact SH3 domain is required for myosin I-induced actin polymerization. EMBO J. 2000;19:4281-91 pubmed
    The yeast type I myosins (MYO3 and MYO5) are involved in endocytosis and in the polarization of the actin cytoskeleton. The tail of these proteins contains a Tail Homology 2 (TH2) domain that constitutes a putative actin-binding site...
  52. Iida K, Yahara I. Cooperation of two actin-binding proteins, cofilin and Aip1, in Saccharomyces cerevisiae. Genes Cells. 1999;4:21-32 pubmed
    Cofilin is a low-molecular weight actin-modulating protein, and is structurally and functionally conserved among eukaryotes. Cofilin is encoded by COF1 in Saccharomyces cerevisiae, and is essential for cell viability...
  53. Malloy L, Wen K, Pierick A, Wedemeyer E, Bergeron S, Vanderpool N, et al. Thoracic aortic aneurysm (TAAD)-causing mutation in actin affects formin regulation of polymerization. J Biol Chem. 2012;287:28398-408 pubmed publisher
    More than 30 mutations in ACTA2, which encodes ?-smooth muscle actin, have been identified to cause autosomal dominant thoracic aortic aneurysm and dissection...
  54. Wen K, Rubenstein P, DeMali K. Vinculin nucleates actin polymerization and modifies actin filament structure. J Biol Chem. 2009;284:30463-73 pubmed publisher
    Vinculin links integrins to the actin cytoskeleton by binding F-actin. Little is known with respect to how this interaction occurs or affects actin dynamics...